BIO RAD VERSADOC User Manual
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BIO RAD VERSADOC User Manual

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VersaDoc
Imaging System
User's Manual
for Catalog Numbers
1708010, 1708011, 1708030
1708031, 1708140, 1708141, 1708050, 1708051
Copyright 2001 Bio-Rad Laboratories Inc.
Manual Part Number 4000183 Rev. E

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  • Page 1  VersaDoc Imaging System User’s Manual for Catalog Numbers 1708010, 1708011, 1708030 1708031, 1708140, 1708141, 1708050, 1708051 Copyright 2001 Bio-Rad Laboratories Inc. Manual Part Number 4000183 Rev. E...
  • Page 3 Imaging System and we are sure that it will provide you with many years of high quality imaging. One of the best ways to familiarize yourself with the capabilities of your new VersaDoc system is to read this manual. In it, you will learn how to set up the system and operate all hardware components.

  • Page 5: Table Of Contents

    3.1.3 Host Computer Recommendations 3.2 System Setup 3.2.1 Unpacking 3.2.2 Shipping Check 3.2.3 Coupling the Camera Module to the Enclosure 3.2.4 Installing VersaDoc 1000,3000,4000 3.2.5 Installing VersaDoc 5000 3.2.6 Installing the Epi Illumination Modules 3-12 3.2.7 Electrical and Communication Connections 3-13 3.2.8 Installing Filters...
  • Page 6 5.2.2 Replacing Lamps in the Epi- illumination Module 5.3 Lens and Filter Storage Section 6 Troubleshooting & Technical Information 6.1 Problem Solving Guide 6.2 Technical Service 6.3 VersaDoc System Specifications 6.4 Warranty Information 6.5 Glossary of Terms 6.6 Parts List...

  • Page 7: About This Manual

    General Information 1.1 About this Manual This manual provides instructions for installing, operating and maintaining the VersaDoc Imaging System. This manual uses certain conventions to facilitate understanding of the text material and to assist operators in using the VersaDoc system.

  • Page 8: Safety Information

    Warning: This instrument must be connected to an appropriate AC voltage outlet that is properly grounded. Warning: There are hazardous voltages inside the rear panel of the VersaDoc . Do not remove the cover to the Electronics back panel when the instrument is connected to AC power.

  • Page 9: Power Safety Information

    AC mains from the unit before performing any disassembly or repair to the instrument. The VersaDoc system is designed and certified to meet EN61010 Safety and EN61326 +A1 Electromagnetic Compliance requirements, which are internationally accepted safety standards. Certified products are safe to use when operated in accordance with the instruction manual.
  • Page 10 Warning: Do not remove the rear access panel when power is supplied to the instrument or defeat the UV safety interlock. Attempting to operate the unit with the cover removed may damage the instrument and expose the operator to UV radiation. Warning: Use of controls or adjustments, or performance of procedures other than those specified herein may result in exposure to hazardous UV radiation.

  • Page 11: Section 2 Introduction

    Flexible Design: The VersaDoc Imaging system has a very flexible design. The light tight enclosure can accommodate any of the offered camera modules. The user has the option to upgrade to any of the four standard models. The standard VersaDoc comes with every feature included.
  • Page 12 (3) Interchangeable Lens The VersaDoc is supplied with two standard lenses. One zoom and one 50 mm mono-focal. Depending upon which VersaDoc system has been purchased, either a 20-40 mm or the 28-80 mm zoom lens will be included.

  • Page 13: Mechanical Description

    Quantity One instruction manual for a full description of this software package. 2.3 Mechanical Description The VersaDoc Imaging System is a modular system and it consists of the following main hardware components. See figure 2.2. 1. The light tight enclosure, which integrates the sliding UV transillumination module, two epi-illumination modules, chemiluminescence tray, access to the filter wheel, lenses and all the electronics needed for controlling the lights and filterwheel.

  • Page 14: Overview Of The Imaging Process

    2.4 Overview of the Imaging Process The acquisition and analysis of image data using VersaDoc technology is a simple five-part process. Step 1: Samples to be imaged are placed on the transilluminator module. Step 2: The appropriate imaging method is selected in the VersaDoc acquisition window of Quantity One.

  • Page 15: Section 3 System Installation

    VersaDoc enclosure from the bottom. The VersaDoc should be placed on a level bench top with a minimum depth of 70 cm and a height clearance of 180 cm, where there is adequate ventilation for the system’s cooling fans to operate.

  • Page 16: Host Computer Recommendations

    3.1.3 Host Computer Recommendations The VersaDoc system is capable of producing large image files of high resolution to easily handle such large files. A powerful computer is required. Please refer to your software manual for detailed host computer system and software requirements.

  • Page 17: Unpacking

    3. Slide the outer sleeve off the box vertically. The VersaDoc enclosure can be found within the box. 4. With the assistance of a helper, remove the VersaDoc from the box. Grip the bottom of the enclosure on both sides (do not lift the instrument by the front door or electronics area) and place it on the floor for the next step in the setup procedure.
  • Page 18 The VersaDoc hardware should arrive complete with the following items: Quantity Item Photo VersaDoc light tight enclosure VersaDoc camera module (Shown Model 1000 camera module, model 3000, 4000 and 5000 are shown later in this section) VersaDoc transilluminator module (already...

  • Page 19: Coupling The Camera Module To The Enclosure

    3.2.3 Coupling the Camera Module to the enclosure: This applies to all camera modules (VersaDoc 1000, 3000, 4000 and 5000). Follow the procedure outlined below to mount the camera module to the enclosure. 1. Leave the enclosure on the floor for easy access to the camera mount.

  • Page 20: Installing Versadoc 1000,3000,4000

    DIN for the VersaDoc 3000. See Figure 3.2 NOTE: VersaDoc 3000 systems with Enclosures that are serial number XXXBR 300 or greater come with a separate power supply for the camera . This power supply already includes a power cable for the VersaDoc 3000 camera.
  • Page 21 Figure 3.2 NOTE: The VersaDoc 4000 camera must be connected to the same power port as the VersaDoc 1000 Connecting the AIA CABLE: For VersaDoc 1000 and 3000 and 4000 only: 1.

  • Page 22: Installing Versadoc 5000

    (CEU) Camera Controller Cable Figure 3.6 2. Couple the VersaDoc 5000 camera module to the enclosure as shown in the section 3.2.3 above. See Figure 3.1 for details. 3. Next connect the CEU to the camera. Caution: The default voltage setting on the camera power supply module is 120 VAC.
  • Page 23 Figure 3.7a Figure 3.7b AIA Connector on CEU Camera Controller Cable Connector Figure 3.7c 2. A small white tab on the AC inlet located on the rear panel indicates the voltage setting on your camera controller unit. See Figure 3.8 Insert a small screw driver and pull out the tab White plastic stub shows the current...
  • Page 24 Small PCB in a tiny slot. This side of the PCB has letters on this side Fuse holder. Figure 3.9 5. Using the plastic stub on the PCB select the orientation of the board so that the desired voltage numbers and arrow point towards the socket for the board. The tab on the inside surface of the stub must match with the notch on the tiny PCB See Figure 3.10a and 3.10b: Plastic stub that...
  • Page 25 9. Check again to make sure that the plastic stub indicates the desired voltage selection Connecting the VersaDoc 5000 Camera and CEU Cables 1. Connect the AIA cable to the connector labeled AIA on the CEU connector panel. The other end of this cable will be connected to the PCI digitizing card in your PC.

  • Page 26: Installing The Epi Illumination Modules

    connections will look similar to the ones shown in Figure 3.13 A or B depending on th eserial number of the enclosure you may have. Fig 3.13A Fig 3.13 B Fan Cable Figure 3.13 3.2.6 Installing the Epi modules: 1. Remove the access panel mounted on the ballast panel by unthreading the two thumbscrews.

  • Page 27: Electrical And Communication Connections

    3.2.7 Electrical and Communication Connections Power The power entry module of the VersaDoc system is configured for operation at 100/120 VAC, 60 Hz or 220-260 VAC, 50 Hz. After checking that the system’s power switch is turned off, insert an approved power cord into the power entry module located on the bottom right of rear panel of the unit.
  • Page 28 Upon insertion of the Serial or USB cable into the VersaDoc, the Serial or USB port the PC will automatically select operation and sense the presence of VersaDoc. NOTE: Do not turn ON the power to the VersaDoc (and CEU in case of VersaDoc 5000) yet! User's Manual • 3-14...

  • Page 29: Software Installation

    The PCI Digitizing card must be installed into the PC where the AIA cable is to be connected Software Installation: Please follow the instructions below to install the PC or Mac software for VersaDoc system: NOTE: Do not install the PCI card into the PC yet. First install the drivers as shown below and then install the PCI Card.
  • Page 30 Click on the arrow next to ChemiDoc XRS/VersaDoc drivers to get installer options. See picture below: d. If you are installing the drivers on local drive select appropriate option by scrolling down as shown in the picture below. Click on “NEXT”:...
  • Page 31 PCI Card is installed before or after installing the software and drivers. Mac based VersaDoc systems NOTE: This procedure applies to both Mac OS 9.xx and OS 10.xx based systems. h. Insert “The Discovery Series” software CD into the CD ROM drive of the MAC.
  • Page 32 Choose custom install from the pulldown menu in this window and click on “INSTALL” The following window will open. Depending upon the Imaging device you are installing, click on the desired Imager you want to install and then click on “Install”. The drivers will get loaded and at the completion of the installation the following window will open User's Manual •...
  • Page 33 Please read the “README” file before using the system Installing the PCI digitizing card to the PC: Caution: Ensure that the VersaDoc system is turned off before Installing the digitizing card and AIA cable. 1. Now install the digitizing card into a PCI slot in your computer and secure it with appropriate screw.
  • Page 34 Power Supply Figure 3.18c Figure 3.18d VersaDoc 3000 Systems starting Serial # xxxBR300 VersaDoc 5000 System starting Serial # xxxBR300 AIA Cable Camera Power Cable Serial/USB Figure 3.18e VersaDoc 1000 and 4000 Systems starting Serial # xxxBR300 User's Manual • 3-20...

  • Page 35: Installing Filters

    VersaDoc as a peripheral device. 1. Power up the VersaDoc system first. 2. Also power up the power supply block in case of the VersaDoc 3000 or CEU in case of the VersaDoc 5000 system. 3. Power up the PC/Mac second.
  • Page 36 5. Remove filter #1 from its packaging and check that it is clean, free of dust, fingerprints and scratches. If the filter is dirty it should be cleaned with the materials provided in the lens and filter cleaning kit. 6. Holding the filter by the numbered tab with the number in the correct orientation, carefully slide the filter into the open filter wheel position.

  • Page 37: Installing The Lens

    When performing any fluorescence experiments it is required that the 660 nm infrared cut-off filter that is supplied with the VersaDoc is installed on the front of collecting lens. This filter will block any infrared signal that will be generated by the UV bulbs, substantially reducing image background and improving sensitivity.
  • Page 38 3. Remove the protective cover from the lens mount (rear of lens) and check that the lens is clean. If the lens is dirty it should be cleaned with the materials provided in the lens and filter cleaning kit. 4. Open the door to the main door and position the lens so that the white line on its mount matches the white mark on the base (right hand side) of the camera assembly.
  • Page 39 Figure 3.21. Steps in 50 mm lens installation. 5. The lens in now locked into position and the lens cap and protective platen cover can be removed. Lens Removal To remove the lens from the camera housing follow the procedure outlined below (Figure 3.22): 1.
  • Page 40 Figure 3.22. Steps in lens removal. Proceed to section 4. User's Manual • 3-26...

  • Page 41: Starting The Versadoc

    After this time has elapsed the host computer can be turned on. At this time you can also power up the power supply or CEU if you have a VersaDoc 3000 or 5000 system. Note: If the LED indicator fails to illuminate and the instrument is inoperative, check that all power cables are firmly attached and that power is being supplied to the unit.

  • Page 42: Detailed Operating Procedures

    From the FILE menu select VERSADOC to open the instruments acquisition window. The following window will open on the screen. Note: If the computer cannot establish communications with the VersaDoc a message will indicate this and give the user the option of entering a simulation mode.

  • Page 43: Selecting The Application

    4.3.3 Placing Samples in the Imaging Chamber The VersaDoc can accommodate a variety of sample types and sizes. The sample stage is 25 X 25 cm. This configuration supports the acquisition of smaller areas of interest within very large samples. The imaging area is liquid sealed so wet samples may be placed directly into the imaging chamber on the UV transilluminator platen.

  • Page 44: Lens Selection And Setup

    4.3.4 Lens Selection and Setup The VersaDoc system is supplied with two standard lenses, a flexible zoom lens and a high numerical aperture 50 mm lens with improved collection efficiency. For optimal image acquisition, it is recommended that the zoom lens with 660 nm cut-off filter installed be used for all fluorescence and colorimetric applications.

  • Page 45: Aperture Adjustment

    The zoom setting can be adjusted and viewed in real time by selecting the POSITION function in the VersaDoc acquisition window. The image display window will now show a real-time image of the sample, as it will be captured. This image refreshes every second to help you optimize your zoom settings.

  • Page 46: Focus Adjustment

    0.5-5 sec. Zoom/IR Sample/Chemi Tray Chemifluorescent Blot 0.5-5 sec. Zoom/IR None Colorimetric Gel 0.1-1 sec. Zoom/IR VersaDoc white light conversion screen Colorimetric Blot 0.1 to 1 sec. Zoom/IR Sample/Chemi Tray X-ray film 0.1-1 sec. Zoom/IR VersaDoc white light conversion screen Weak 5-10 min.

  • Page 47: Acquiring The Image

    In this application, the software will first take an initial image and then prompt you to remove the sample. NOTE: Please make sure that the surface of the VersaDoc white light conversion screen is clean after the sample has been removed.

  • Page 48: Care And Maintenance Page

    Never wear powdered gloves when cleaning the VersaDoc. Clean the sample platen with optical cleaning solution and a lint free optical tissue. Cleaning kits are available from Bio-Rad and one is supplied with the instrument.

  • Page 49: Cleaning The Lens

    5.1.3 Cleaning the Lens To optimize image quality it is recommended that any lens used with the VersaDoc be cleaned before it is installed in the system. Both sides of the lens should be cleaned using an optical cleaning solution and optical tissue. Avoid touching the glass surfaces of the lens when installing it into the instrument, as fingerprints will effect image quality.
  • Page 50 the guides rails located in the enclosure and the entire transilluminator module will become free from the enclosure. Release lever for slides Transilluminator Transilluminator without the cover Figure 5.1 6. Using the Phillips screwdriver, remove the cover assembly from the transilluminator by removing four screws.

  • Page 51: Replacing Lamps In The Epi-Illumination Module

    NOTE: The procedure to remove and replace the lamp in the UV or white light epi modules is the same. The procedure outlined below applies to both. 1. Turn off the VersaDoc and disconnect all power, removing the power cable. 2. Open the door to the sample compartment.

  • Page 52: Lens And Filter Storage

    6. This exposes the lamps in the epi module for you to replace. To replace the white light lamp or UV lamp simply grab the two metal ends of the old lamp and carefully rotate to release it from the sockets. See Figure 5.4 Remove lamp by grabbing the two metal ends and turning the lamp to release from its socket Figure 5.4 16.

  • Page 53: Section 6 Troubleshooting & Technical Information

    VersaDoc Door is open Close Door Serial/USB or AIA cable is not Reconnect Serial/USB/AIA cables connected properly and ensure it is seated properly...
  • Page 54 Problem Possible Cause Solution Fluorescent image has Image was taken without being Remove plate from sample. high background removed from glass plate Thin polyacrylamide samples can be transferred to exposed x-ray film and imaged using UV-epi mode 660 NM filter is not in place Install 660 NM filter onto lens Wrong application selected Verify / reselect correct...

  • Page 55: Technical Service

    6.2 Technical Service For technical assistance with the VersaDoc system including all hardware and software, contact your local Bio-Rad office, or in the US call 1 800 424 6723. All spare parts not listed in this document can be ordered by contacting your local Bio-Rad office.

  • Page 56: Versadoc System Specifications

    Appendix 1 6.3 VersaDoc System Specifications System Technical Specifications Specification Light source Transillumination UV (290 - 365 NM) and white light (400 – 750 Epi illumination UV (290 - 365 NM) and white light (400 – 750 Emission filters 8 position filter wheel: 520LP, 530DF70, 610LP...
  • Page 57 VersaDoc 4000 Cooled CCD Specification Imaging array 2184 X 1472 Pixel size 6.8 x 6.8 micron Pixel depth 12bit Detector type Scientific grade, high sensitivity front illuminated CCD with antireflective coating and micro lenses Cooling system Forced air peltier thermoelectric system 10°C ( ±...

  • Page 58: Warranty Information

    For the exact terms of warranty, please see the Instrument Warranty Card. In the event that the VersaDoc must be returned to the factory for repair under warranty, the instrument must be packed and returned in its original shipping container.

  • Page 59: Glossary Of Terms

    6.5 Glossary of Imaging Terms CCD: Charge Coupled Device. CCD Element: Each CCD element or pixel is a single photodetector capable of detecting light and converting and storing the information in an electronic form. CCD Array: A CCD array can be visualized as a periodic grid array of individual CCD elements, (analogous to buckets of water).

  • Page 60: Parts List

    6.6 Spare Parts List: Part Number Description 8000247 Cable, Camera AIA 9210780 Cable, Camera DC Power, VersaDoc 1000 9310070 Cable, Serial PC,DB9M 9310071 Cable, USB, Type A to B 9310072 Cable, Serial, MAC, DIN8 TO DB9M 9000213* Lamp, EPI White Light...
  • Page 61 Bio-Rad Laboratories Life Science Web site www.bio-rad.com Bio-Rad Laboratories Main Office 2000 Alfred Nobel Drive, Hercules, CA 94547, Ph. (510) 741-1000, Fx. (510) 741-5800 Group Also in: Australia Ph. 02 9914 2800, Fx. 02 9914 2889 Austria Ph. (01) 877 89 01, Fx. (01) 876 56 29 Belgium Ph. 09-385 55 11, Fx. 09-385 65 54 Brazil Ph.

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