Nikon ECLIPSE E200 POL Instructions Manual page 28

14
Circular Graduated Stage for Polarizing Microscope
Loosening the stage rotation clamp screw can
rotate the stage. The angle of rotation can be read
to 0.1 degrees using the vernier.
Centering the stage
(1) Focus on the specimen with 10x objective.
Remove the analyzer from the optical path.
Match the rotating center of the stage and the
intersection point of the eyepiece crosshairs
in the following way.
<1> Move the slide glass on the stage to
move a distinct point on the specimen
(such as a grain) to the center of the
intersection point of the eyepiece
crosshairs.
<2> Loosen the stage rotation clamp screw
and rotate the stage for 180 degrees.
<3> If the distinct point shifts away from the
center, rotate two stage centering
screws so that the intersection point of
the eyepiece crosshairs moves halfway
toward the distinct point.
<4> Repeat steps <2> and <3> several
times.
(2) Bring the next large magnification objective
into the optical path and perform the
centering in the same way. Then bring the
next large magnification objective and do the
same till the centering procedure is performed
on all the objectives in order including the
objective of the highest magnification. (In the
normal set, 40x objective is the next large
objective of the 10x objective. The 100x
objective is available in option.)
(3) From the above procedure, the rotating
center of the stage and the intersection point
of the eyepiece crosshairs coincides when
observed with the highest magnification
objective. (When the objective of other
magnification is used, the rotating center
deviates a little from the intersection point of
the eyepiece crosshairs but stays inside the
viewfield.)
IV Microscopy (Detailed Procedure)
26
After 180°
rotation
Distinct
point
Vernier
Stage rotation
clamp screw
Halfway
position of
the deviation