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Replacing a Specimen Using the
Try focusing on the specimen with 40x or higher magnification objective. You will find the
specimen is brought very near to the objective.
without moving the focus knob. In a case like this, use the refocusing mechanism for easy
specimen replacement.
(1)
Use one hand to gently press down the
stage.
*2
(2)
While holding the stage at that position,
change the specimen.
(3)
Gradually release the stage so that it
rises slowly. The stage will return to the
focal position.
*1:
The distance between the front of the objective and the specimen when the specimen is in focus is
called the "working distance" of the objective. For details, see p. 42.
*2:
When lowering the stage, take great care not to hit the field lens with the condenser and the parts
under the condenser.
IV
Microscopy (Detailed Procedure)
Refocusing Mechanism
*1
It will be very difficult to change the specimen
15
IV
Make sure not
to hit the field
lens.
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