Adjusting Transmitted-Light Phase Contrast Or Transmitted-Light Darkfield - Zeiss Primo Star Operating Manual

Primo Star
3.3.5 Adjusting transmitted-light phase
contrast or transmitted-light
darkfield
• First, adjust the microscope as you do for
brightfield.
• Turn the nosepiece to swing the phase-contrast
objective (40x/Ph 2) into the light path.
• Open the luminous-field diaphragm (Fig. 9/3, if
adjustable) on the stand and open the aperture
diaphragm by means of control lever (Fig. 9/1)
on Abbe condenser (Fig. 9/2).
• If the phase-contrast slider (Fig. 9/6) was not
factory-installed yet, first unscrew its screw
(Fig. 9/7). Then, push the slider from the left
into the Abbe condenser and screw the screw
(Fig. 9/7) back in again.
• Push the slider to the right as far as it will go to
move the phase stop into the light path.
• Move the control lever of the aperture
diaphragm to fully open it on the Full Köhler
model or set it to the PH mark on the Fixed
Köhler model.
• Adjust the illumination intensity as needed.
• Check the centering of the phase stop
according to the drawing in Fig. 10. To this end,
remove one eyepiece and replace it by the
eyepiece telescope.
• Center the phase stop, if necessary, (Fig. 10/A)
by turning the two adjusting screws of the
slider (Fig. 9/5) by means of the two Allen keys
SW 1.5 (Fig. 9/4) until the phase stop image
corresponds to that shown in Fig. 10/B.
• Afterwards, replace the eyepiece telescope by
the eyepiece again.
For darkfield applications, use the darkfield slider in place of the phase-contrast slider.
B 46-0071 v 03/06
Start-Up and Operation
Fig. 9
Fig. 10
Inserting the slider
Centering the phase stop
Carl Zeiss
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