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Detailed view of excitation light balancer
(1)
Ａ
Transmission rate
100%
A
50%
0%
350
The excitation light balancer is set so that the transmission rate remains at about 100% for
typical dark fluorescent FITC.
Position of optical path
(1)
Between (1) and (2)
(2)
Between (2) and (3)
(3)
About objectives
Lamp voltage: If accurate color reproduction is critical, push in the preset switch on the
microscope, set the NCB11 filter into the optical path, and use ND filters to make brightness
adjustments.
Plan Fluor
S Fluor
Plan Apo
Individual Operations
19 Excitation Light Balancer
B
Ｂ
(2)
(3)
A
B
Ｂ
FITC
DAPI
400
450
DAPI
100％
Variable (100% - 50%)
50%
Variable (50% - 0%)
0%
40x/0.75
40x/0.9
40x/0.95
35
Effective diameter on aperture
diaphragm plane
B
A
TRITC
Texas-Red
500
550
600
FITC
100%
100%
100%
100%
100%
40xH/1.3
100xH/1.3
40xH/1.3
100xH/1.3
60xH/1.4
100xH/1.4
650
700
TRITC/Texas-Red
0%
Variable (0% - 50%)
50%
Variable (50% - 100%)
100%
Wavelength
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