Hints For Successful Use Of Solvents And Mobile Phase - Agilent Technologies 1200 Series User Manual

3 Becoming an expert

Hints for Successful use of Solvents and Mobile Phase

Always filter solvents through 0.4 µm filters, small particles can permanently
block the capillaries, the valves and damage or cause corrosion of parts.
• Avoid the use of alkaline solutions (pH > 8.5) which can attack the fused
• Avoid exposure of the solvent bottles to direct sunlight.
• Avoid the use of the following steel-corrosive solvents:
• Mobile phase for Nanoflow and proteomics, as a starting point should
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silica from the capillaries.
• Solutions of alkali halides and their respective acids (for example,
lithium iodide, potassium chloride, and so on).
• High concentrations of inorganic acids like sulfuric and nitric acid,
especially at higher temperatures (replace, if your chromatography
method allows, by phosphoric acid or phosphate buffer which are less
corrosive against stainless steel).
• Halogenated solvents or mixtures which form radicals and/or acids, for
example:
• 2CHCl + O & 2COCl
3 2
• This reaction, in which stainless steel probably acts as a catalyst, occurs
quickly with dried chloroform if the drying process removes the
stabilizing alcohol.
• Chromatographic grade ethers, which can contain peroxides (for
example, THF, dioxane, di-isopropylether) such ethers should be filtered
through dry aluminium oxide which adsorbs the peroxides.
• Solvents containing strong complexing agents (e.g. EDTA).
• Mixtures of carbon tetrachloride and 2-propanol dissolve stainless steel.
• Mixture of THF and 2-Propanol, can dissolve stainless steel.
consist of a mobile phase A,B and a needle wash solvent. The composition
would be as follows:
• A = 0.1% Formic acid
• B = 0.1% Formic acid
• Needle wash = 15% Methanol, 84.9 %Water, 0.1 % Formic acid.
+ 2HCl
2
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