Agilent Technologies 1200 Infinity Series User Manual
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Agilent Technologies 1200 Infinity Series User Manual

Diode array detectors
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Diode Array Detectors
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  • Page 1 Agilent 1200 Infinity Series Diode Array Detectors User Manual Agilent Technologies...
  • Page 2 1987) or any equivalent agency regulation or contract clause. Use, duplication or dis- closure of Software is subject to Agilent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will Agilent 1200 Infinity Series DAD User Manual...
  • Page 3 Overview about the troubleshooting and diagnostic features. 7 Error Information This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. Agilent 1200 Infinity Series DAD User Manual...
  • Page 4 This chapter describes the detector in more detail on hardware and electronics. 13 LAN Configuration This chapter provides information on connecting the module to the Agilent ChemStation PC. 14 Appendix This chapter provides addition information on safety, legal and web. Agilent 1200 Infinity Series DAD User Manual...

  • Page 5: Table Of Contents

    Main Screens of the Detector with Agilent Instant Pilot (G4208A) Solvent Information 5 Optimizing the Detector Introduction Optimization Overview Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Optimizing Selectivity Optimizing the Detector Regarding to the System Warm up of the Detector Agilent 1200 Infinity Series DAD User Manual...
  • Page 6 D/A Converter (DAC) Test Dark Current Test 9 Maintenance Warnings and Cautions Introduction to Maintenance Overview of Maintenance Cleaning the Module Replacing the Deuterium Lamp Replacing the Max-Light Cartridge Cell Cleaning of Max-Light Cartridge Cell Agilent 1200 Infinity Series DAD User Manual...
  • Page 7 What You Have to Do First TCP/IP parameter configuration Configuration Switch Initialization mode selection Dynamic Host Configuration Protocol (DHCP) Link configuration selection Automatic Configuration with BootP Manual Configuration PC and Agilent ChemStation Setup Agilent 1200 Infinity Series DAD User Manual...
  • Page 8 Contents 14 Appendix Safety The Waste Electrical and Electronic Equipment Directive Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet Agilent 1200 Infinity Series DAD User Manual...

  • Page 9: Introduction

    Agilent 1200 Infinity Series DAD User Manual Introduction Overview of the Module Optical System Lamp Max-Light Cartridge Flow Cell Slit Assembly Grating and Diode Array Bio-inert Materials This chapter gives an introduction to the detector and an instrument overview. Agilent Technologies...

  • Page 10: Overview Of The Module

    • Additional diagnostic signals for temperature and lamp voltage monitoring. • Easy exchange of flow cell by cartridge design. For specifications, see “Performance Specifications G4212A” on page 24 or “Performance Specifications G4212B” on page 26. Agilent 1200 Infinity Series DAD User Manual...

  • Page 11: Optical System

    [4] through the slit assembly [5] onto a holographic grating [6] light being dispersed onto the diode array [7]. This allows simultaneous access to all wavelength information. Agilent 1200 Infinity Series DAD User Manual...
  • Page 12 The light source for the UV-wavelength range is a long-life UV-lamp with RFID tag. As a result of plasma discharge in low-pressure deuterium gas, the lamp emits light over the 190 nm to approximately 800 nm wavelength range. Figure 2 UV-Lamp Agilent 1200 Infinity Series DAD User Manual...
  • Page 13 Max-Light Cartridge Flow Cell For additional information on the Max-Light Cartridge flow cell refer to “Choosing a Flow N O T E Cell” on page 80 and “Inline Pressure Relief Valve Kit (G4212-68001)” on page 82. Agilent 1200 Infinity Series DAD User Manual...
  • Page 14 The slit width is directly controlled by the micro-processor of the instrument and can be set as method parameter. Figure 4 Slit Assembly The slit width influences the spectral resolution and noise. Agilent 1200 Infinity Series DAD User Manual...
  • Page 15 In March 2011 the optical unit type of the G4212B DAD changed from "Programmable Slit N O T E (like in the G4212A) fixed to 4 nm" to a real "Fixed Slit 4 nm". First serial number was DEAA301100. Agilent 1200 Infinity Series DAD User Manual...
  • Page 16 The diode array is a series of 1024 individual photodiodes and control circuits located on a ceramic carrier. It has a wavelength range from 190 – 640 nm and the sampling interval is ∼0.5 nm. Figure 6 Grating and diode array Agilent 1200 Infinity Series DAD User Manual...

  • Page 17: Bio-Inert Materials

    Bio-inert Materials Bio-inert Materials For the Agilent 1260 Infinity Bio-inert LC system, Agilent Technologies uses highest quality materials in the flow path (also referred to as wetted parts), which are widely accepted by life scientists, as they are known for optimum inertness to biological samples and ensure best compatibility with common samples and solvents over a wide pH range.
  • Page 18 PEEK, fused silica (G5615-60018) and Max-Light Cartridge Cell Bio-inert ( 60 mm, V(σ) 4.0 µL) (G5615-60017) (for Agilent 1200 Infinity Series Diode Array Detectors DAD G4212A/B) Bio-inert flow cell, 8 µL, 20 bar (pH 1–12) includes Capillary Kit PEEK, fused silica, PTFE...

  • Page 19: Site Requirements And Specifications

    Agilent 1200 Infinity Series DAD User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications Specifications Specification Conditions This chapter provides information on environmental requirements, physical and performance specifications. Agilent Technologies...

  • Page 20: Site Requirements

    ➔ Make sure the power connector of the instrument can be easily reached and unplugged. ➔ Provide sufficient space behind the power socket of the instrument to unplug the cable. Agilent 1200 Infinity Series DAD User Manual...
  • Page 21 Never operate your instrumentation from a power outlet that has no ground connection. ➔ Never use a power cord other than the Agilent Technologies power cord designed for your region. Use of unsupplied cables WA R N I N G Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury.
  • Page 22 This module is designed to operate in a typical electromagnetic environment, i.e. where RF N O T E transmitters such as mobile telephones may not be used in close proximity. Agilent 1200 Infinity Series DAD User Manual...

  • Page 23: Physical Specifications

    Up to 2000 m (6562 ft) Non-operating altitude Up to 4600 m (15091 ft) For storing the module Safety standards: Installation category II, Pollution degree 2 For indoor use only. IEC, CSA, UL Agilent 1200 Infinity Series DAD User Manual...

  • Page 24: Performance Specifications

    Programmable in steps of 1 nm bunching Slit width 1, 2, 4, 8 nm Programmable slit Diode width ~ 0.5 nm Signal data rate up to 160 Hz Spectra Data rate up to 160 Hz Agilent 1200 Infinity Series DAD User Manual...
  • Page 25 Maximum Operating Pressure (MOP): The maximum pressure at which the system can operate continuously under normal conditions. Maximum Incidential Pressure (MIP): The maximum pressure which the system can experience during a short time. Agilent 1200 Infinity Series DAD User Manual...
  • Page 26 Wavelength 2 – 400 nm Programmable in steps of 1 nm bunching Slit width 4 nm Fixed slit Diode width ~ 0.5 nm Signal data rate 80 Hz Spectra Data rate 80 Hz Agilent 1200 Infinity Series DAD User Manual...
  • Page 27 Maximum Operating Pressure (MOP): The maximum pressure at which the system can operate continuously under normal conditions. Maximum Incidential Pressure (MIP): The maximum pressure which the system can experience during a short time. Agilent 1200 Infinity Series DAD User Manual...

  • Page 28: Specification Conditions

    Time Constant versus Response Time According to ASTM E1657-98 „Standard Practice of Testing Variable-Wavelength Photometric Detectors Used in Liquid Chromatography” the time constant is converted to response time by multiplying by the factor 2.2. Agilent 1200 Infinity Series DAD User Manual...

  • Page 29: Installing The Module

    Agilent 1200 Infinity Series DAD User Manual Installing the Module Unpacking the Module Damage to the module Delivery Checklist Detector Accessory Kit Contents Optimizing the Stack Configuration One Stack Configuration Two Stack Configuration Installing the Detector Flow Connections to the Detector...

  • Page 30: Unpacking The Module

    Damage to the module Damaged Packaging If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. "Defective on arrival" problems C A U T I O N If there are signs of damage, please do not attempt to install the module.

  • Page 31: Delivery Checklist

    Unpacking the Module Delivery Checklist Ensure all parts and materials have been delivered with the module. The delivery checklist is shown below. Please report missing or damaged parts to your local Agilent Technologies sales and service office. Table 5 Detector Checklist Description...

  • Page 32: Optimizing The Stack Configuration

    System, you can ensure optimum performance by installing the following configurations. These configurations optimize the system flow path, ensuring minimum delay volume. For other possible configurations, please refer to the Agilent 1260 Infinity/1290 Infinity LC System Manual. Agilent 1200 Infinity Series DAD User Manual...

  • Page 33: One Stack Configuration

    33 and Figure 8 on page 34). This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required. Figure 7 Recommended Stack Configuration for 1260 Infinity (Front View) Agilent 1200 Infinity Series DAD User Manual...
  • Page 34 Installing the Module Optimizing the Stack Configuration Figure 8 Recommended Stack Configuration for 1260 Infinity (Rear View) Agilent 1200 Infinity Series DAD User Manual...
  • Page 35 The Agilent 1290 Infinity Binary Pump should always be installed at the bottom of the stack. Figure 9 Recommended stack configuration for 1290 Infinity with binary pump (front view) Agilent 1200 Infinity Series DAD User Manual...
  • Page 36 Installing the Module Optimizing the Stack Configuration Figure 10 Recommended stack configuration 1290 Infinity with binary pump (rear view) Agilent 1200 Infinity Series DAD User Manual...

  • Page 37: Two Stack Configuration

    A slightly longer capillary is required between the pump and autosampler. (See Figure 11 on page 37 and Figure 12 on page 38). Figure 11 Recommended Two Stack Configuration for 1260 Infinity (Front View) Agilent 1200 Infinity Series DAD User Manual...
  • Page 38 Installing the Module Optimizing the Stack Configuration Figure 12 Recommended Two Stack Configuration for 1260 Infinity (Rear View) Agilent 1200 Infinity Series DAD User Manual...
  • Page 39 A slightly longer capillary is required between the pump and autosampler. (See Figure 13 on page 39 and Figure 14 page 40). Figure 13 Recommended two stack configuration for 1290 Infinity with binary pump (front view) Agilent 1200 Infinity Series DAD User Manual...
  • Page 40 Installing the Module Optimizing the Stack Configuration Figure 14 Recommended two stack configuration for 1290 Infinity with binary pump (rear view) Agilent 1200 Infinity Series DAD User Manual...

  • Page 41: Installing The Detector

    Make sure that it is always possible to access the power plug. ➔ Remove the power cable from the instrument before opening the cover. ➔ Do not connect the power cable to the Instrument while the covers are removed. Agilent 1200 Infinity Series DAD User Manual...
  • Page 42 The module is turned on when the line power switch is pressed and the green indicator N O T E lamp is illuminated. The module is turned off when the line power switch is protruding and the green light is off. Agilent 1200 Infinity Series DAD User Manual...
  • Page 43 N O T E optical unit and controlling the temperature. This is described in “Warm up of the Detector” on page 107. Give the optical unit enough time to warm-up and stabilize (> 60 minutes). Agilent 1200 Infinity Series DAD User Manual...

  • Page 44: Flow Connections To The Detector

    “Optimizing the Stack Configuration” on page 32. Remove the front cover. Remove the black hoods from the cell interfaces (in/out) and store them in the plastic case provided with the Max-Light Cartridge Flow Cell. Agilent 1200 Infinity Series DAD User Manual...
  • Page 45 Locate the flow cell cartridge. Unlock the flow cell cartridge by pulling the lever to the front. The lever should be in the final down position. Pull the flow cell cartridge completely out towards the front. Agilent 1200 Infinity Series DAD User Manual...
  • Page 46 Lift the two levers into the upper final position to fix the Remove the plugs from the CELL-IN and CELL-OUT (keep cell. them safe). Agilent 1200 Infinity Series DAD User Manual...
  • Page 47 CELL-IN and CELL-OUT should be closed with the plugs. To protect the flow cell against overpressure please refer to “Inline Pressure Relief Valve Kit N O T E (G4212-68001)” on page 82. Agilent 1200 Infinity Series DAD User Manual...

  • Page 48: Initial Recalibration

    • significant environmental condition changes (temperature, humidity) during transport and storage, • significant environmental condition changes (temperature, humidity) in final location and • variances between factory test cell and the installed flow cell. Agilent 1200 Infinity Series DAD User Manual...

  • Page 49: Using The Module

    Agilent 1200 Infinity Series DAD User Manual Using the Module Preparing the Detector Setting up the Detector with Agilent ChemStation The Detector GUI Control Settings Method Parameter Settings General Method Settings Advanced Method Parameter Settings Spectrum Settings Other Advanced Method Parameter Settings...

  • Page 50: Preparing The Detector

    • Do not work with removed front panels. When the front panel of the G1316C TCC (typically located below the detector) is removed while the TCC is set to high temperatures, the up-streaming air could influence the stability of the detector baseline. Agilent 1200 Infinity Series DAD User Manual...

  • Page 51: Setting Up The Detector With Agilent Chemstation

    ChemStation or other Agilent 1260 Infinity/1290 Infinity Series modules refer to the corresponding documentation or system manual. After successful load of the ChemStation, you should see the module as an active item in the graphical user interface (GUI). Agilent 1200 Infinity Series DAD User Manual...
  • Page 52 Using the Module Setting up the Detector with Agilent ChemStation Figure 16 ChemStation Method & Run Agilent 1200 Infinity Series DAD User Manual...

  • Page 53: The Detector Gui

    • Signal name (A, B, C, ...) • Sample wavelength/bandwidth • Reference wavelength/bandwidth • Absorbance If more signals are activated, the size of the detector GUI will change accordingly. Agilent 1200 Infinity Series DAD User Manual...
  • Page 54 Ready text” or “Error text”. • Error (Red) • Not ready (yellow) • Ready (green) • Pre run, Post run (purple) • Run (blue) • Idle (green) • Offline (dark gray) • Standby (light gray) Agilent 1200 Infinity Series DAD User Manual...
  • Page 55 The information provides flow cell and lamp related information like • Part number • Production date • Serial number and other details. Agilent 1200 Infinity Series DAD User Manual...

  • Page 56: Control Settings

    Non-RFID-tag lamp In case a non-RFID-tag lamp is used, the user interface will show this when selecting a compatible mode. You may operate the detector outside of the guaranteed specification. Agilent 1200 Infinity Series DAD User Manual...

  • Page 57: Method Parameter Settings

    N O T E right mouse click on the detector GUI. Switches to additional instrument signals for troubleshooting Toggles to Advanced Settings (actual window) Toggles to Timetable Settings Opens the Time table graph Agilent 1200 Infinity Series DAD User Manual...

  • Page 58: General Method Settings

    < 0.0025 min/ < 0.15 sec). N O T E The 1290 Infinity DAD (G4212A) has a data rate of up to 160 Hz. The 1260 Infinity DAD (G4212B) has a data rate of up to 80 Hz. Agilent 1200 Infinity Series DAD User Manual...
  • Page 59 (As Pump/Injector) or the module (if different from system stop time). The data collection is stopped at this time. A posttime period can be used to allow module’s items to equilibrate (e.g. after gradient change or temperature change). Agilent 1200 Infinity Series DAD User Manual...

  • Page 60: Advanced Method Parameter Settings

    Parameter Settings (in case the Time Table Settings are open). This screen shows the default settings. Figure 17 Method Parameter Settings The 1260 Infinity DAD (G4212B) has a fixed slit width of 4 nm. N O T E Agilent 1200 Infinity Series DAD User Manual...

  • Page 61: Spectrum Settings

    Range defines the wavelength range for spectral storage. Limits: 190 to 640 nm in steps of 1 nm for both low and high values. The high value must be greater than the low value by at least 2 nm. Agilent 1200 Infinity Series DAD User Manual...
  • Page 62 The threshold is the height in mAU of the smallest expected peak. The peak detector ignores any peaks which are lower than the threshold value and does not save spectra. Limits: 0.001 to 1000.00 mAU in steps of 0.001 mAU. Agilent 1200 Infinity Series DAD User Manual...

  • Page 63: Other Advanced Method Parameter Settings

    Defines, whether a balance is performed prior to a run and/or after a run has finished. Lamp on required for analysis If unchecked, the lamp will be turned off after the analysis has finished. Agilent 1200 Infinity Series DAD User Manual...

  • Page 64: Time Table Settings

    You may set up time events to change functions with their parameters over the run time. Add lines as required. Limits: 0.00 to 99999.00 minutes in steps of 0.01 min. Parameter Based on the chosen function, a certain parameter can be selected. Agilent 1200 Infinity Series DAD User Manual...
  • Page 65 Using the Module Setting up the Detector with Agilent ChemStation Time Table Graph In this view the activated signals are shown how they change according the time table. Agilent 1200 Infinity Series DAD User Manual...

  • Page 66: Instrument Curves

    These signals can be used in addition to the normal baseline signal to determine whether correlation to temperature or voltage/current of the lamp. These signals are available via the Agilent ChemStation Online Plot/Data Signal and/or Agilent Lab Advisor Software. Agilent 1200 Infinity Series DAD User Manual...

  • Page 67: Instrument Configuration

    Setting up the Detector with Agilent ChemStation Instrument Configuration These settings are available via menu Instrument – Instrument Configuration. Figure 18 Menu Instrument Configuration Via the Instrument Configuration screen additional modules can be added to a system. Agilent 1200 Infinity Series DAD User Manual...
  • Page 68 This results in a change of features and functions. Serial number: based on the module. Firmware revision: based on the module. Options: lists installed options. Agilent 1200 Infinity Series DAD User Manual...

  • Page 69: Main Screens Of The Detector With Agilent Instant Pilot (G4208A)

    Below the main screens for the use of the detector are shown. The Welcome screen shows all modules of the system. The Control screen allows • Lamp On/Off • Get Ready • Reset of Errors • Balance • Take Spectrum Agilent 1200 Infinity Series DAD User Manual...
  • Page 70 The Configuration screen allows to configure • Symbolic name of module • Temperature control • Lamp and cell RFID tag use • Analog Output range • UV lamp at power on • LAN settings Agilent 1200 Infinity Series DAD User Manual...
  • Page 71 Using the Module Main Screens of the Detector with Agilent Instant Pilot (G4208A) The Method screen lists all method parameters of the detector. These can be edited. Agilent 1200 Infinity Series DAD User Manual...
  • Page 72 • logging of maintenance activities • module identification (blinking LED) Firmware Updates can be done via the System Maintenance screen. The Diagnose screen provides access to module specific tests • Lamp Intensity • Calibration • Cell Agilent 1200 Infinity Series DAD User Manual...

  • Page 73: Solvent Information

    Solvent information for parts of the 1260 Infinity Bio-inert LC system For the Agilent 1260 Infinity Bio-inert LC system, Agilent Technologies uses highest quality materials (see “Bio-inert Materials” on page 17) in the flow...
  • Page 74 It is corroded by strong bases and should not be used above pH 12 at room temperature. The corrosion of flow cell windows can negatively affect measurement results. For a pH greater than 12, the use of flow cells with sapphire windows is recommended. Agilent 1200 Infinity Series DAD User Manual...
  • Page 75 Most data available refers to room temperature (typically 20 – 25 °C, 68 – 77 °F). If corrosion is possible, it usually increases at higher temperatures. If in doubt, consult additional resources. Agilent 1200 Infinity Series DAD User Manual...
  • Page 76 Using the Module Solvent Information Agilent 1200 Infinity Series DAD User Manual...

  • Page 77: Optimizing The Detector

    Agilent 1200 Infinity Series DAD User Manual Optimizing the Detector Introduction Optimization Overview Choosing a Flow Cell Inline Pressure Relief Valve Kit (G4212-68001) Application Information Special Information of 60 mm Cartridge Flow Cell Special Information for Bio-Inert Cartridge Flow Cells...

  • Page 78: Introduction

    How to Get the Best Detector Performance The information below will guide you on how to get the best detector performance. Follow these rules as a start for new applications. It gives rules-of-thumb for optimizing detector parameters. Agilent 1200 Infinity Series DAD User Manual...

  • Page 79: Optimization Overview

    • Set the spectral wavelength range (for colorless samples 190...400 nm is sufficient). • Set step to 4 nm for normal use; set small step (and slit width) if high resolution of spectra with fine structure is wanted. Agilent 1200 Infinity Series DAD User Manual...

  • Page 80: Choosing A Flow Cell

    If higher sensitivity is necessary, the Max-Light Cartridge Cell ( 60 mm, V(σ) 4.0 μL) (G4212-60007) can be used. This cell enhances the detector by lowering the limit of detection (LOD) by a factor of about 3 (depending on the application). Agilent 1200 Infinity Series DAD User Manual...
  • Page 81 The use of Peek-FS capillaries is not recommended. In combination with the SST zero dead volume fitting (e.g. at the inlet) the capillary could break and the glass particles could block/damage the flow cell. Agilent 1200 Infinity Series DAD User Manual...

  • Page 82: Inline Pressure Relief Valve Kit (G4212-68001)

    For applications with mobile phases of a pH above 12.5 use an Agilent Diode Array Detector G1315C/D and the respective bio-inert flow cell. Agilent 1200 Infinity Series DAD User Manual...

  • Page 83: Special Information Of 60 Mm Cartridge Flow Cell

    (digital and analog) is normalized to 1 cm. This means the peak height would be the same as on the 10 mm Cartridge Flow Cell, the noise is reduced by a factor of 6 and the linearity limit would be 333 mAU/cm. Agilent 1200 Infinity Series DAD User Manual...

  • Page 84: Special Information For Bio-Inert Cartridge Flow Cells

    For applications with mobile phases of a pH above 12.5 use an Agilent 1260 Infinity Diode Array Detector G1315C/D and the respective bio-inert flow cell. Agilent 1200 Infinity Series DAD User Manual...

  • Page 85: Optimizing For Sensitivity, Selectivity, Linearity And Dispersion

    When increasing the path length, the cell volume could increase. Depending on the peak volume, this could cause more peak dispersion. Agilent 1200 Infinity Series DAD User Manual...
  • Page 86 Agilent detector it is necessary to have more precise information on path lengths of the detector flow cells. σ Part Number Path Length Cell Volume ( G4212-60008/G5615-60018 1.0 cm 1.0 µL G4212-60007/G5615-60017 6.0 cm 4.0 µL Agilent 1200 Infinity Series DAD User Manual...

  • Page 87: Peak Width (Response Time)

    (response time) by factor of two from the recommended setting (over-filtering) will reduce peak height by about 20 % and reduce baseline noise by a factor of 1.4. This gives you the best possible signal-to-noise ratio, but may affect peak resolution. Agilent 1200 Infinity Series DAD User Manual...
  • Page 88 The maximum spectra scan rate depends on the data points per scan, see Table 8 N O T E page 88. Running at 160 Hz, the spectra scan data rate is reduced automatically if the spectra scan data rate is more than 251 points/scan. Agilent 1200 Infinity Series DAD User Manual...

  • Page 89: Sample And Reference Wavelength And Bandwidth

    360,100 is adequate. Anisic acid does not absorb in this range. If you work with high concentrations, you may get better linearity above 1.5 AU by setting the sample wavelength to a valley in the spectrum, like 225 nm for anisic acid. Agilent 1200 Infinity Series DAD User Manual...
  • Page 90 2.5, whereas the signal is about 75 % of a 4 nm wide band. The signal-to-noise ratio for a 30 nm bandwidth is twice that for a 4 nm bandwidth in our example. Figure 21 Influence of Bandwidth on Signal and Noise Agilent 1200 Infinity Series DAD User Manual...
  • Page 91 With this technique, PTH-amino acids can be quantified in the low picomole range even in a gradient analysis. Figure 22 Gradient Analysis of PTH-Amino Acids (1 pmol each), with and without Reference Agilent 1200 Infinity Series DAD User Manual...

  • Page 92: Slit Width (G4212A)

    Benzene at 1 and 4 nm slit width (principle) A wide slit uses more of the light shining through the flow cell. This gives lower baseline noise as shown in Figure 24 on page 93. Agilent 1200 Infinity Series DAD User Manual...
  • Page 93 Use a wide (8 nm) slit when your sample contains very small concentrations. Always use signals with bandwidth at least as wide as the slit width. Agilent 1200 Infinity Series DAD User Manual...

  • Page 94: Optimizing Spectral Acquisition

    4 nm would be sufficient. However a step of 2 nm gives a more optimal display of the spectrum. Threshold Sets the peak detector. Only spectra from peaks higher than threshold will be stored when a peak-controlled storage mode is selected. Agilent 1200 Infinity Series DAD User Manual...

  • Page 95: Margin For Negative Absorbance

    (about -100 mAU). In some special case, for example, when gradient with absorbing solvents are used, the baseline may drift to more negative values. Only for such cases, increase the margin for negative absorbance to avoid overflow of the analog-to-digital converter. Agilent 1200 Infinity Series DAD User Manual...

  • Page 96: Optimizing Selectivity

    96 shows, hydrochlorothiazide is best detected at 222 nm, where caffeine also shows significant absorbance. It would therefore be impossible, with a conventional variable wavelength detector, to detect hydrochlorothiazide quantitatively when caffeine is present. Figure 25 Wavelength Selection for Peak Suppression Agilent 1200 Infinity Series DAD User Manual...
  • Page 97 The trade-off for this procedure is a loss in sensitivity. The sample signal decreases by the absorbance at the reference wavelength relative to the signal wavelength. Sensitivity may be decreased by as much as 10–30 %. Figure 26 Peak Suppression Using Reference Wavelength Agilent 1200 Infinity Series DAD User Manual...

  • Page 98: Ratio Qualifiers For Selective Detection Of Compound Classes

    An example is shown in Figure 27 on page 98 and Figure 28 on page 99. Figure 27 Wavelength Selection for Ratio Qualifiers Agilent 1200 Infinity Series DAD User Manual...
  • Page 99 249 and 224 nm was within this range, is the signal plotted. Of all four peaks, only the third fulfilled the criterion (Figure 28 on page 99). The others were not plotted. Agilent 1200 Infinity Series DAD User Manual...

  • Page 100: Optimizing The Detector Regarding To The System

    60 mm Max-Light high sensitivity cell is used to get higher sensitivity the cell volume is optimized for the use with 3 mm and 4.6 mm inner diameter columns. Agilent 1200 Infinity Series DAD User Manual...

  • Page 101: How To Achieve Higher Sensitivity

    The Max-Light high sensitivity cell has a path length of 60 mm and this will give between three and five times increase in signal-to-noise values depending on the application conditions. The dispersion volume is fractionally increased compared to the standard cell. Agilent 1200 Infinity Series DAD User Manual...
  • Page 102 12 nm generally gives good sensitivity and is specific for absorbance in a narrow range. The narrow band can be optimized for sensitivity for a specific molecule. As the bandwidth is increased the signal is reduced but so is the noise and there Agilent 1200 Infinity Series DAD User Manual...
  • Page 103 If there is no part of the spectrum with zero absorbance then it will be better to have the reference signal turned off. Agilent 1200 Infinity Series DAD User Manual...
  • Page 104 Optimizing the Detector Optimizing the Detector Regarding to the System Figure 29 Spectrum of Anisic Acid Agilent 1200 Infinity Series DAD User Manual...
  • Page 105 The full spectra is not available under all conditions. N O T E Based on the data points, the scan data rate is reduced, see Table 9 on page 106. Agilent 1200 Infinity Series DAD User Manual...
  • Page 106 The maximum spectra scan rate depends on the data points per scan, see Table 9 N O T E page 106. Running at 160 Hz, the spectra scan data rate is reduced automatically if the spectra scan data rate is more than 251 points/scan. Agilent 1200 Infinity Series DAD User Manual...

  • Page 107: Warm Up Of The Detector

    The example below was done under stable environmental conditions. The figures below show the first two hours of a detector warm-up phase. The lamp was turned on immediately after turn on of the detector. Agilent 1200 Infinity Series DAD User Manual...
  • Page 108 Optimizing the Detector Warm up of the Detector Figure 30 Detector Warm-up – 1 hour Figure 31 Detector Warm-up – 2 hour Agilent 1200 Infinity Series DAD User Manual...

  • Page 109: Troubleshooting And Diagnostics

    Agilent 1200 Infinity Series DAD User Manual Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Status Indicators Power Supply Indicator Module Status Indicator Available Tests vs User Interfaces Agilent Lab Advisor Software Intermittent Issues Loose Optical Cable Board Type does not match when replacing the main board Overview about the troubleshooting and diagnostic features.

  • Page 110: Overview Of The Module's Indicators And Test Functions

    The module has several signals (internal temperatures, voltages and currents of lamps) that can be used for diagnosing baseline problems. These can be added like normal signals in the Agilent ChemStation software. For details see “Instrument Curves” on page 66. Agilent 1200 Infinity Series DAD User Manual...

  • Page 111: Status Indicators

    Figure 32 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch. When the indicator is illuminated (green) the power is ON. Agilent 1200 Infinity Series DAD User Manual...

  • Page 112: Module Status Indicator

    In such a case try to re-boot the module or try a cold-start (see “Special Settings” on page 219). Then try a firmware update (see “Replacing Module Firmware” on page 183). If this does not help, a main board replacement is required. Agilent 1200 Infinity Series DAD User Manual...

  • Page 113: Available Tests Vs User Interfaces

    “Agilent Lab Advisor Software” on page 114. • The Agilent ChemStation B.04.02 and above may not include any maintenance/test functions. • Screenshots used within these procedures are based on the Agilent Lab Advisor software. Agilent 1200 Infinity Series DAD User Manual...

  • Page 114: Agilent Lab Advisor Software

    Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules. This includes diagnostic capabilities, calibration procedures and maintenance routines for all the maintenance routines. The Agilent Lab Advisor software also allows users to monitor the status of their LC instruments.

  • Page 115: Intermittent Issues

    115) and the main board. If this is loose, intermittent or constant effects can be seen. Do not start replacements (optical or main board) before above check. N O T E Figure 33 Location of Cover at Optical Unit Agilent 1200 Infinity Series DAD User Manual...

  • Page 116: Board Type Does Not Match When Replacing The Main Board

    • During boot of the DAD it retrieves the module type (G4212A or G4212B DAD) from the installed optical unit and keeps it. • Via Board Check and Change add the serial number. • Reboot the DAD (if not done automatically). Agilent 1200 Infinity Series DAD User Manual...

  • Page 117: Error Information

    Agilent 1200 Infinity Series DAD User Manual Error Information What Are Error Messages General Error Messages Timeout Shutdown Remote Timeout Lost CAN Partner Leak Sensor Short Leak Sensor Open Compensation Sensor Open Compensation Sensor Short Fan Failed Leak Open Cover...

  • Page 118: What Are Error Messages

    In all cases, error propagation is done via the CAN bus or via an APG remote cable (see documentation for the APG interface). Agilent 1200 Infinity Series DAD User Manual...

  • Page 119: General Error Messages

    Check the logbook for the occurrence and A not-ready condition was present during a source of a not-ready condition. Restart the sequence or multiple-injection run for a analysis where required. period longer than the timeout threshold. Agilent 1200 Infinity Series DAD User Manual...

  • Page 120: Shutdown

    Exchange the remote cable. Defective remote cable. Check the instrument for defects (refer to the Defective components in the instrument instrument’s documentation). showing the not-ready condition. Agilent 1200 Infinity Series DAD User Manual...

  • Page 121: Lost Can Partner

    • Please contact your Agilent service Leak sensor incorrectly routed, being representative. pinched by a metal component. • Correct the routing of the cable. • If cable defective, exchange the leak sensor. Agilent 1200 Infinity Series DAD User Manual...

  • Page 122: Leak Sensor Open

    If the resistance across the sensor increases above the upper limit, the error message is generated. Probable cause Suggested actions Please contact your Agilent service Defective main board. representative. Agilent 1200 Infinity Series DAD User Manual...

  • Page 123: Compensation Sensor Short

    Probable cause Suggested actions Please contact your Agilent service Fan cable disconnected. representative. Please contact your Agilent service Defective fan. representative. Please contact your Agilent service Defective main board. representative. Agilent 1200 Infinity Series DAD User Manual...

  • Page 124: Leak

    Error ID: 0205 The top foam has been removed. Probable cause Suggested actions Please contact your Agilent service Foam not activating the sensor. representative. Please contact your Agilent service Defective sensor or main board. representative. Agilent 1200 Infinity Series DAD User Manual...

  • Page 125: Cover Violation

    Probable cause Suggested actions Please contact your Agilent service The top foam was removed during representative. operation. Please contact your Agilent service Foam not activating the sensor. representative. Agilent 1200 Infinity Series DAD User Manual...

  • Page 126: Detector Error Messages

    Ensure the UV lamp connector is seated firmly. Lamp disconnected. Exchange the UV lamp. Defective UV lamp or non-Agilent lamp. Please contact your Agilent service representative. Defective detector main board. Please contact your Agilent service representative. Defective power supply. Agilent 1200 Infinity Series DAD User Manual...

  • Page 127: Uv Lamp Voltage

    Ensure the lamp is connected. Lamp disconnected. Exchange the UV lamp. Defective UV lamp or non-Agilent lamp. Please contact your Agilent service representative. Defective detector main board. Please contact your Agilent service representative. Defective power supply. Agilent 1200 Infinity Series DAD User Manual...

  • Page 128: Uv Heater Current

    ROM, the error message is generated. Probable cause Suggested actions Please contact your Agilent service Defective connector or cable. representative. Please contact your Agilent service Defective PDA/optical unit. representative. Agilent 1200 Infinity Series DAD User Manual...

  • Page 129: Wavelength Recalibration Lost

    Probable cause Suggested actions Please contact your Agilent service Defective sensor or main board. representative. Verify that the ambient conditions are within Detector is exposed to illegal ambient the allowed range. conditions. Agilent 1200 Infinity Series DAD User Manual...

  • Page 130: Illegal Temperature Value From Sensor At Fan Assembly

    If the test fails an error event is created. As a result the temperature control is switched off. Probable cause Suggested actions Please contact your Agilent service Defective connector or cable. representative. Please contact your Agilent service Defective heater. representative. Agilent 1200 Infinity Series DAD User Manual...

  • Page 131: Heater Power At Limit

    0 means upper power limit hit (excessive ambient temperature drop). 1 means lower power limit hit (excessive ambient temperature increase). Probable cause Suggested actions Wait until temperature control equilibrates. Excessive ambient temperature change. Agilent 1200 Infinity Series DAD User Manual...
  • Page 132 Error Information Detector Error Messages Agilent 1200 Infinity Series DAD User Manual...

  • Page 133: Test Functions And Calibration

    Agilent 1200 Infinity Series DAD User Manual Test Functions and Calibration Introduction Use of Max-Light Cartridge Test Cell Conditions of Detector Failing a Test Self-Test Intensity Test Test Failed Cell Test Test Failed (low ratio value) Quick Noise Test Test Failed...

  • Page 134: Introduction

    • Lamp anode voltage signals possible Agilent Instant Pilot Some tests are available • Intensity • WL Calibration • Cell For details on the use of the interface refer to the interface documentation. Agilent 1200 Infinity Series DAD User Manual...

  • Page 135: Use Of Max-Light Cartridge Test Cell

    Max-Light Cartridge Cell Bio-inert 60 mmV( ) = 4 µL σ G4212-60032 100 % Max-Light Cartridge Cell HDR (3.7 mm, V( ) 0.4 µL) σ G4212-60017 100 % Max-Light Cartridge Cell ULD (10 mm, V( ) 0.6 µL) Agilent 1200 Infinity Series DAD User Manual...

  • Page 136: Conditions Of Detector

    (not active during the first 30 minutes after turn on). If the detector is on, tests can be performed usually 10 minutes after the UV-lamp has been turned on. Agilent 1200 Infinity Series DAD User Manual...

  • Page 137: Failing A Test

    If a test fails with the Max-Light Cartridge Cell repeat the test with the Max-Light Cartridge Test Cell and compare. If the test fails also, then start with proposed actions mentioned in the details of the tests. Agilent 1200 Infinity Series DAD User Manual...

  • Page 138: Self-Test

    • Lamp must be on for at least 10 minutes. • For noise test a longer warm-up time may be required (> 2 hours). • When using a Max-Light Cartridge Cell a flow rate of 0.5 mL/min with water is required. Agilent 1200 Infinity Series DAD User Manual...
  • Page 139 Test Functions and Calibration Self-Test 1 Run the Self-Test with the recommended user interface (for further information see Online-Help of user interface). Figure 34 Self-Test – Results Agilent 1200 Infinity Series DAD User Manual...

  • Page 140: Intensity Test

    Therefore, intensity spectra will differ slightly between instruments. When In case of UV-lamp problem (drift, noise). Parts required Description Max-Light Cartridge Cell (filled with water) Max-Light Cartridge Test Cell Preparations Lamp must be on for at least 10 minutes. Agilent 1200 Infinity Series DAD User Manual...
  • Page 141 Test Functions and Calibration Intensity Test 1 Run the Intensity-Test with the recommended user interface (for further information see Online-Help of user interface). Figure 35 Intensity Test – Results Figure 36 Intensity Test – Signals Agilent 1200 Infinity Series DAD User Manual...

  • Page 142: Test Failed

    Cell and new UV-lamp, please contact your Agilent service representative. If only one range fails and the application does not require this range, the lamp may not be N O T E changed. Agilent 1200 Infinity Series DAD User Manual...

  • Page 143: Cell Test

    • When using a Max-Light Cartridge Cell a flow rate of 0.5 mL/min with water is required. 1 Run the Cell-Test with the recommended user interface (for further information see Online-Help of user interface). Figure 37 Cell Test – Results Agilent 1200 Infinity Series DAD User Manual...

  • Page 144: Test Failed (Low Ratio Value)

    Ensure the flow cell is filled with water, and free Absorbing solvent or air bubble in flow cell. from air bubbles. Clean the flow cell as described in “Cleaning of Dirty or contaminated flow cell. Max-Light Cartridge Cell” on page 178. Agilent 1200 Infinity Series DAD User Manual...

  • Page 145: Quick Noise Test

    • Detector and UV-lamp must be on for at least 2 hours. • ASTM measurements based on specifications may require longer stabilization times. • When using a Max-Light Cartridge Cell a flow rate of 0.5 mL/min with water is required. Agilent 1200 Infinity Series DAD User Manual...
  • Page 146 1 Run the Quick Noise Test with the recommended user interface (for further information see Online-Help of user interface). Figure 39 Quick Noise Test – Results Figure 40 Quick Noise Test — Signals Agilent 1200 Infinity Series DAD User Manual...

  • Page 147: Test Failed

    Absorbing solvent or air bubble in flow cell. from air bubbles. • Flush flow cell. Dirty or contaminated flow cell. • Clean the flow cell as described in “Cleaning of Max-Light Cartridge Cell” page 178. Exchange the UV-lamp. Old UV-lamp. Agilent 1200 Infinity Series DAD User Manual...

  • Page 148: Astm Drift And Noise Test

    • Detector and UV-lamp must be on for at least 2 hours. • ASTM measurements based on specifications may require longer stabilization times. • When using a Max-Light Cartridge Cell a flow rate of 0.5 mL/min with water is required. Agilent 1200 Infinity Series DAD User Manual...
  • Page 149 1 Run the ASTM Drift and Noise Test with the recommended user interface (for further information see Online-Help of user interface). Figure 41 ASTM Drift and Noise Test – Results Figure 42 ASTM Drift and Noise Test – Signals Agilent 1200 Infinity Series DAD User Manual...

  • Page 150: Test Failed

    • Flush flow cell. Dirty or contaminated flow cell. • Clean the flow cell as described in “Cleaning of Max-Light Cartridge Cell” page 178. Exchange the UV-lamp. Old UV-lamp. Improve environment. Environment not according to specifications. Agilent 1200 Infinity Series DAD User Manual...

  • Page 151: Slit Test

    • When using a Max-Light Cartridge Cell a flow rate of 0.5 mL/min with water is required. 1 Run the Slit Test with the recommended user interface (for further information see Online-Help of user interface). Figure 43 Slit Test – Results Agilent 1200 Infinity Series DAD User Manual...

  • Page 152: Test Failed

    Run the “Intensity Test”. Exchange the lamp if old Old lamp. or defective. Please contact your Agilent service representative. Defective slit assembly. Please contact your Agilent service representative. Defective detector main board. Please contact your Agilent service representative. Defective optical unit. Agilent 1200 Infinity Series DAD User Manual...

  • Page 153: Slit Test (G4212B)

    There is no dedicated slit test for the G4212B DAD. To verify the proper function perform the following tests: • Intensity Test (tests the normal position) • Dark Current Test (tests the dark position) Agilent 1200 Infinity Series DAD User Manual...

  • Page 154: Wavelength Verification Test

    Max-Light Cartridge Test Cell or Max-Light Cartridge Cell Preparations • Lamp must be on for at least 10 minutes. • When using a Max-Light Cartridge Cell a flow rate of 0.5 mL/min with water is required. Agilent 1200 Infinity Series DAD User Manual...
  • Page 155 Test Functions and Calibration Wavelength Verification Test 1 Run the Wavelength Verification Test with the recommended user interface (for further information see Online-Help of user interface). Figure 45 Wavelength Verification - Results Agilent 1200 Infinity Series DAD User Manual...

  • Page 156: Wavelength Calibration

    Max-Light Cartridge Test Cell or Max-Light Cartridge Cell Preparations • Detector/lamp must be on for more than 1 hour. • When using a Max-Light Cartridge Cell a flow rate of 0.5 mL/min with water is required. Agilent 1200 Infinity Series DAD User Manual...
  • Page 157 10 minutes after lamp on. A final wavelength calibration should be repeated after complete warm-up of the detector. 1 Run the Wavelength Calibration with the recommended user interface (for further information see Online-Help of user interface). Figure 46 Wavelength Calibration – Results Agilent 1200 Infinity Series DAD User Manual...

  • Page 158: Wavelength Recalibration Fails

    See also “Cleaning of Max-Light Cartridge Cell” on page 178. If the test fails with Max-Light Cartridge Test Cell and new UV-lamp, the optical unit must N O T E be replaced. Agilent 1200 Infinity Series DAD User Manual...

  • Page 159: D/A Converter (Dac) Test

    Lamp must be on for at least 10 minutes. Connect integrator, chart recorder or data system to the detector analog output. Running the test with Agilent LabAdvisor 1 Run the D/A Converter (DAC) Test (for further information see Online-Help of user interface). Figure 47 D/A Converter (DAC) Test – Results Agilent 1200 Infinity Series DAD User Manual...

  • Page 160: Test Evaluation

    The noise on the step should be less than 3 μV. Probable cause Suggested actions Check or replace the cable. Bad cable or grounding problem between detector and external device. Please contact your Agilent service Defective detector main board. representative. Agilent 1200 Infinity Series DAD User Manual...

  • Page 161: Dark Current Test

    (represented in counts) for each diode should fall within the limits. When In case of problem. 1 Run the Dark Current Test with the recommended user interface (for further information see Online-Help of user interface). Figure 49 Dark Current Test – Results Agilent 1200 Infinity Series DAD User Manual...

  • Page 162: Test Failed

    151 (part Defective slit assembly (stray light). of the “Self-Test” on page 138). Please contact your Agilent service Defective detector main board. representative. Please contact your Agilent service Defective PDA/optical unit. representative. Agilent 1200 Infinity Series DAD User Manual...

  • Page 163: Maintenance

    Agilent 1200 Infinity Series DAD User Manual Maintenance Warnings and Cautions Introduction to Maintenance Overview of Maintenance Cleaning the Module Replacing the Deuterium Lamp Replacing the Max-Light Cartridge Cell Cleaning of Max-Light Cartridge Cell Storage of Max-Light Cartridge Cell Drying the Leak Sensor...

  • Page 164: Warnings And Cautions

    Repair work at the module can lead to personal injuries, e.g. shock hazard, when the cover is opened. ➔ Do not remove the cover of the module. ➔ Only certified persons are authorized to carry out repairs inside the module. Agilent 1200 Infinity Series DAD User Manual...
  • Page 165 C A U T I O N ➔ If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment. Agilent 1200 Infinity Series DAD User Manual...

  • Page 166: Introduction To Maintenance

    The module is designed for easy maintenance. Maintenance can be done from the front with module in place in the system stack. There are no serviceable parts inside. N O T E Do not open the module. Agilent 1200 Infinity Series DAD User Manual...

  • Page 167: Overview Of Maintenance

    If leaking or if intensity drops due to contaminated flow A wavelength calibration test should be cell. performed after replacement. Leak sensor drying If leak has occurred. Check for leaks. Leak handling System If broken or corroded. Check for leaks. replacement Agilent 1200 Infinity Series DAD User Manual...

  • Page 168: Cleaning The Module

    WA R N I N G hazard and damage the module ➔ Do not use an excessively damp cloth during cleaning. ➔ Drain all solvent lines before opening any connections in the flow path. Agilent 1200 Infinity Series DAD User Manual...

  • Page 169: Replacing The Deuterium Lamp

    WA R N I N G If the detector has been in use, the lamp may be hot. ➔ If so, wait for lamp to cool down. Remove the front cover. Locate the lamp area. Agilent 1200 Infinity Series DAD User Manual...
  • Page 170 Disconnect the lamp connector and remove the lamp. Place the lamp on a clean place. N O T E Do not touch the glass bulb with your fingers. It may reduce the light output. Agilent 1200 Infinity Series DAD User Manual...
  • Page 171 Locate the two screws and fix the lamp. Fit the lamp wires in the lamp house cover so that they Replace the lamp housing cover and fix the 3 screws. are not scratched by the cover. Agilent 1200 Infinity Series DAD User Manual...
  • Page 172 Maintenance Replacing the Deuterium Lamp Close the front cover. Perform a Wavelength Re-calibration after lamp warm-up. Agilent 1200 Infinity Series DAD User Manual...

  • Page 173: Replacing The Max-Light Cartridge Cell

    The flow cell is shipped with a filling of isopropanol. This is to avoid breakage due to N O T E subambient conditions. In case the flow cell is not used for some time (stored), then flush the flow cell with iso-propanol. Agilent 1200 Infinity Series DAD User Manual...
  • Page 174 Remove the front cover. Locate the cell area. Disconnect the inlet capillary to CELL-IN (left) and the Unlock the cell cartridge holder by pulling the lever to the waste tubing to CELL-OUT (right). front. Agilent 1200 Infinity Series DAD User Manual...
  • Page 175 For longer storage, the flow cell should be flushed and filled with isopropanol to prevent the grow of algae. Store it in the plastic case provided with the Max-Light Cartridge Flow Cell. Agilent 1200 Infinity Series DAD User Manual...
  • Page 176 Lift the two levers into the upper final position to fix the Remove the plugs from the CELL-IN and CELL-OUT (keep cell. them safe). Agilent 1200 Infinity Series DAD User Manual...
  • Page 177 Maintenance Replacing the Max-Light Cartridge Cell Connect the inlet capillary to CELL-IN (left) and the waste Close the front cover. tubing to CELL-OUT (right). Agilent 1200 Infinity Series DAD User Manual...

  • Page 178: Cleaning Of Max-Light Cartridge Cell

    5 If tests fail again, the flow cell might be replaced if the chromatographic performace cannot be accepted. If the cleaning with the alcohol did not improve, you may use Cell cleaning fluid, 1 L N O T E (5062-8529). Agilent 1200 Infinity Series DAD User Manual...

  • Page 179: Storage Of Max-Light Cartridge Cell

    2 Remove the Max-Light Cartridge Cell from the cartridge holder of the detector. 3 Replace the black hoods, that secure the cell light inlet and outlet. 4 Store the Max-Light Cartridge Cell in plastic case provided with the Max-Light Cartridge Flow Cell. Agilent 1200 Infinity Series DAD User Manual...

  • Page 180: Drying The Leak Sensor

    Dry the leak sensor and the area around. Check loose Close the front cover. fittings at the flow cell. Note that the leak sensor does not touch the panel (clearance of about 1 mm). Agilent 1200 Infinity Series DAD User Manual...

  • Page 181: Replacing Leak Handling System Parts

    5061-3388 Leak funnel 5041-8389 Leak funnel holder 5062-2463 Corrugated tubing, PP, 6.5 mm id, 5 m G4212-40027 Leak downpipe Preparations Turn the pump off. Remove the front cover. Locate the leak interface area. Agilent 1200 Infinity Series DAD User Manual...
  • Page 182 Pull the leak funnel out of the leak funnel holder and slide Insert the leak interface system parts. Ensure the tubing the Leak downpipe up for removal. is fitted correctly in the bottom. Close the front cover. Agilent 1200 Infinity Series DAD User Manual...

  • Page 183: Replacing Module Firmware

    1 Download the required module firmware, the latest LAN/RS-232 FW Update Tool and the documentation from the Agilent web. http://www.chem.agilent.com/_layouts/agilent/downloadFirmware.aspx?whid=69761 • 2 For loading the firmware into the module follow the instructions in the documentation. Agilent 1200 Infinity Series DAD User Manual...
  • Page 184 Introduced 07/2012. Firmware B.06.51, B.06.43 or B.06.26 or later (depends on the used firmware Optical set). Earlier revisions are not compatible with the VSA Optical. These revisions are the required versions for the new VSA Optical Unit and Main Boards. Conversion to / emulation Agilent 1200 Infinity Series DAD User Manual...

  • Page 185: Information From Module's Assemblies

    N O T E e.g. PSI. Serial Number and Firmware Revision The user interface provides module specific information that is stored in the main board. These are for example the serial number, firmware revision. Agilent 1200 Infinity Series DAD User Manual...
  • Page 186 Maintenance Information from Module’s Assemblies Agilent 1200 Infinity Series DAD User Manual...

  • Page 187: Parts And Materials For Maintenance

    Agilent 1200 Infinity Series DAD User Manual Parts and Materials for Maintenance Overview of Maintenance Parts Kits Accessory Kit Inline Pressure Relief Valve Kit (G4212-68001) This chapter provides information on parts for maintenance. Agilent Technologies...

  • Page 188: Overview Of Maintenance Parts Kits

    Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste) G4212-40027 Leak downpipe G4208A Instant Pilot Instant Pilot G4208A (requires firmware B.02.11 or above). N O T E For cables refer to “Cable Overview” on page 192. Agilent 1200 Infinity Series DAD User Manual...
  • Page 189 Parts and Materials for Maintenance Overview of Maintenance Parts Agilent 1200 Infinity Series DAD User Manual...
  • Page 190 CAN cable, Agilent module to module, 0.5 m Inline Pressure Relief Valve Kit (G4212-68001) To protect the flow cell against overpressure please refer to “Inline Pressure Relief Valve Kit N O T E (G4212-68001)” on page 82. Agilent 1200 Infinity Series DAD User Manual...

  • Page 191: Identifying Cables

    Agilent 1200 Infinity Series DAD User Manual Identifying Cables Cable Overview Analog Cables Remote Cables BCD Cables CAN/LAN Cables RS-232 Cables This chapter provides information on cables used with the Agilent 1260 Infinity/1290 Infinity LC modules. Agilent Technologies...

  • Page 192: Cable Overview

    Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent module to 3394/6 integrators...
  • Page 193 It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m Agilent 1200 Infinity Series DAD User Manual...

  • Page 194: Analog Cables

    Signal Name module Not connected Shield Analog - Center Analog + Agilent Module to BNC Connector p/n 8120-1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent 1200 Infinity Series DAD User Manual...
  • Page 195 Identifying Cables Analog Cables Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog + Agilent 1200 Infinity Series DAD User Manual...

  • Page 196: Remote Cables

    Identifying Cables Remote Cables Remote Cables One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Agilent Module to 3396A Integrators...
  • Page 197 6 - Yellow 6 - Yellow Power on High 7 - Red 7 - Red Ready High 8 - Green 8 - Green Stop 9 - Black 9 - Black Start request Agilent 1200 Infinity Series DAD User Manual...
  • Page 198 Wire Color Pin Agilent Signal Name Active module (TTL) White Digital ground Brown Prepare run Gray Start Blue Shut down Pink connected Yellow Power on High Ready High Green Stop Black Start request Agilent 1200 Infinity Series DAD User Manual...

  • Page 199: Bcd Cables

    BCD 0 Orange BCD 3 BCD 2 Brown BCD 1 Gray Digital ground Gray Gray/pink BCD 11 Red/blue BCD 10 White/green BCD 9 Brown/green BCD 8 not connected not connected + 5 V Agilent 1200 Infinity Series DAD User Manual...
  • Page 200 Agilent Module to 3396 Integrators p/n 03396-60560 Pin 3396 Pin Agilent Signal Name BCD Digit module BCD 5 BCD 7 BCD 6 BCD 4 BCD0 BCD 3 BCD 2 BCD 1 Digital ground + 5 V Agilent 1200 Infinity Series DAD User Manual...

  • Page 201: Can/Lan Cables

    CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) Agilent 1200 Infinity Series DAD User Manual...

  • Page 202: Cables

    It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m Agilent 1200 Infinity Series DAD User Manual...

  • Page 203: Hardware Information

    Agilent 1200 Infinity Series DAD User Manual Hardware Information Firmware Description Electrical Connections Rear view of the module Information on Instrument Serial Number Interfaces Overview Interfaces Setting the 8-bit Configuration Switch Special Settings Instrument Layout Early Maintenance Feedback This chapter describes the detector in more detail on hardware and electronics.

  • Page 204: Firmware Description

    • run synchronization through APG remote, • error handling, • diagnostic functions, • or module specific functions like internal events such as lamp control, filter movements, • raw data collection and conversion to absorbance. • Agilent 1200 Infinity Series DAD User Manual...
  • Page 205 Update of main system can be done in the resident system only. Update of the resident N O T E system can be done in the main system only. Main and resident firmware must be from the same set. Figure 51 Firmware Update Mechanism Agilent 1200 Infinity Series DAD User Manual...
  • Page 206 All these specific informations are described in the documentation provided with the firmware update tools. The firmware update tools, firmware and documentation are available from the Agilent web. http://www.chem.agilent.com/_layouts/agilent/downloadFirmware.aspx?whid=69761 • Agilent 1200 Infinity Series DAD User Manual...

  • Page 207: Electrical Connections

    There are no externally accessible fuses, because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.

  • Page 208: Rear View Of The Module

    Rear view of the module Figure 52 Rear View of Detector – Electrical Connections and Label The CompactFlash Card Slot is not active yet. It may be used for future enhancements. N O T E Agilent 1200 Infinity Series DAD User Manual...

  • Page 209: Information On Instrument Serial Number

    • CN = China Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module) 00000 Serial number Agilent 1200 Infinity Series DAD User Manual...

  • Page 210: Interfaces

    Hardware Information Interfaces Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces: Table 15 Agilent 1200 Infinity Series Interfaces Module LAN/BCD RS-232 Analog Special (optional) (on-board) Remote Pumps G1310B Iso Pump G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump K1312B Bin Pump Clinical Ed.
  • Page 211 K1321B FLD Clinical Ed. G1362A RID G4280A ELSD EXT Contact AUTOZERO Others G1170A Valve Drive G1316A/C TCC K1316C TCC Clinical Ed. G1322A DEG K1322A DEG Clinical Ed. G1379B DEG G4225A DEG K4225A DEG Clinical Ed. Agilent 1200 Infinity Series DAD User Manual...
  • Page 212 • CAN connectors as interface to other modules • LAN connector as interface to the control software • RS-232C as interface to a computer • REMOTE connector as interface to other Agilent products • Analog output connector(s) for signal output Agilent 1200 Infinity Series DAD User Manual...

  • Page 213: Overview Interfaces

    There is no configuration possible on main boards with on-board LAN. These are N O T E pre-configured for • 19200 baud, • 8 data bit with no parity and • one start bit and one stop bit are always used (not selectable). Agilent 1200 Infinity Series DAD User Manual...
  • Page 214 RS-232C Connection Table Direction Function Ground Figure 53 RS-232 Cable Analog Signal Output The analog signal output can be distributed to a recording device. For details refer to the description of the module’s main board. Agilent 1200 Infinity Series DAD User Manual...
  • Page 215 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
  • Page 216 START REQUEST (L) Request to start injection cycle (for example, by start key on any module). Receiver is the autosampler. Special Interfaces There is no special interface for this module. Agilent 1200 Infinity Series DAD User Manual...

  • Page 217: Setting The 8-Bit Configuration Switch

    • For boot/test modes switches 1+2 must be UP plus required mode. For normal operation use the default (best) settings. N O T E Figure 54 Location of Configuration Switch (example shows a G4212A DAD) Agilent 1200 Infinity Series DAD User Manual...
  • Page 218 When selecting the mode TEST, the LAN settings are: Auto-Negotiation & Using Stored. N O T E For explanation of "Boot Resident System" and "Revert to Default Data (Coldstart)" refer to N O T E “Special Settings” on page 219. Agilent 1200 Infinity Series DAD User Manual...

  • Page 219: Special Settings

    Save your methods and data before executing a forced cold start. If you use the following switch settings and power the instrument up again, a forced cold start has been completed. Table 20 Forced Cold Start Settings (On-board LAN) Mode Select TEST/BOOT Agilent 1200 Infinity Series DAD User Manual...

  • Page 220: Instrument Layout

    • the plastic layers help cushion the electronic and mechanical parts from physical shock, and • the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself. Agilent 1200 Infinity Series DAD User Manual...

  • Page 221: Early Maintenance Feedback

    EMF limits, and then reset the EMF counters to zero. The next time the EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. Agilent 1200 Infinity Series DAD User Manual...
  • Page 222 Hardware Information Early Maintenance Feedback Agilent 1200 Infinity Series DAD User Manual...

  • Page 223: Lan Configuration

    Agilent 1200 Infinity Series DAD User Manual LAN Configuration What You Have to Do First TCP/IP parameter configuration Configuration Switch Initialization mode selection Dynamic Host Configuration Protocol (DHCP) General Information (DHCP) Setup (DHCP) Link configuration selection Automatic Configuration with BootP...

  • Page 224: What You Have To Do First

    224) to the PC network card using a crossover network cable (point-to-point) or • a hub or switch using a standard LAN cable. • Figure 56 Location of LAN interfaces and MAC label Agilent 1200 Infinity Series DAD User Manual...

  • Page 225: Tcp/Ip Parameter Configuration

    TCP/IP parameters after power-on. The parameters may be derived from a Bootp cycle, non-volatile memory or initialized with known default values. The initialization mode is selected by the configuration switch, see Table 22 on page 227. Agilent 1200 Infinity Series DAD User Manual...

  • Page 226: Configuration Switch

    Initialization (‘Init’) Mode Bootp, all switches down. For details see “Initialization mode selection” on page 227 Link Configuration speed and duplex mode determined by auto-negotiation, for details see “Link configuration selection” on page 234 Agilent 1200 Infinity Series DAD User Manual...

  • Page 227: Initialization Mode Selection

    Bootp Server. The parameters obtained become the active parameters immediately. They are not stored to the non-volatile memory of the module. Therefore, the parameters are lost with the next power cycle of the module. Figure 58 Bootp (Principle) Agilent 1200 Infinity Series DAD User Manual...
  • Page 228 Use the initialization mode Bootp & Store carefully, because writing to the non-volatile N O T E memory takes time. Therefore, when the module shall obtain its parameters from a Bootp Server every time it is powered on, the recommended initialization mode is Bootp! Agilent 1200 Infinity Series DAD User Manual...
  • Page 229 Using the default address in your local area network may result in network problems. Take N O T E care and change it to a valid address immediately. Table 23 Using Default Parameters IP address: 192.168.254.11 Subnet Mask: 255.255.255.0 Default Gateway not specified Agilent 1200 Infinity Series DAD User Manual...
  • Page 230 In the Using Default mode, the parameters stored in the memory of the module are not N O T E cleared automatically. If not changed by the user, they are still available, when switching back to the mode Using Stored. Agilent 1200 Infinity Series DAD User Manual...

  • Page 231: Dynamic Host Configuration Protocol (Dhcp)

    2 It may be necessary to fully qualify the hostname with the DNS suffix, e.g. 0030d3177321.country.company.com. 3 The DHCP server may reject the hostname proposed by the card and assign a name following local naming conventions. Agilent 1200 Infinity Series DAD User Manual...

  • Page 232: Setup (Dhcp)

    Card or the main board of above mentioned modules. Table 24 G1369C LAN Interface Card (configuration switch on the card) SW 4 SW 5 SW 6 SW 7 SW 8 Initialization Mode DHCP Agilent 1200 Infinity Series DAD User Manual...
  • Page 233 4 Configure your Control Software (e.g. Agilent ChemStation, LabAdvisor, Firmware Update Tool) and use MAC address as host name, e.g. 0030d3177321. The LC system should become visible in the control software (see Note in section “General Information (DHCP)” on page 231). Agilent 1200 Infinity Series DAD User Manual...

  • Page 234: Link Configuration Selection

    Link Configuration speed and duplex mode determined by auto-negotiation manually set to 10 Mbps, half-duplex manually set to 10 Mbps, full-duplex manually set to 100 Mbps, half-duplex manually set to 100 Mbps, full-duplex Agilent 1200 Infinity Series DAD User Manual...

  • Page 235: Automatic Configuration With Bootp

    BootP Service is installed on a server or PC on the LAN to provide central administration of IP addresses for Agilent instruments on a LAN. The BootP service must be running TCP/IP network protocol and cannot run a DHCP server. Agilent 1200 Infinity Series DAD User Manual...

  • Page 236: How Bootp Service Works

    • Does the Firewall block the BootP service? Add the BootP service as an exception. • Is the LAN Interface using the BootP-mode instead of "Using Stored" or "Using Default" modes? Agilent 1200 Infinity Series DAD User Manual...

  • Page 237: Installation Of Bootp Service

    9 The Destination Folder selection screen appears. Install BootP to the default folder or click Browse to choose another location. Click Next. The default location for installation is: C:\Program Files\Agilent\BootPService\ 10 Click Install to begin installation. Agilent 1200 Infinity Series DAD User Manual...
  • Page 238 16 Start BootP Service in the Windows® services: On the Windows® desktop click right on Computer icon, select Manage > Services and Applications > Services. Select the Agilent BootP Service and click Start. Agilent 1200 Infinity Series DAD User Manual...

  • Page 239: Two Methods To Determine The Mac Address

    243 or “Restarting the Agilent BootP Service” on page 244. 5 Uncheck the Do you want to log BootP requests? box after configuring instruments; otherwise, the log file will quickly fill up disk space. Agilent 1200 Infinity Series DAD User Manual...

  • Page 240: Assigning Ip Addresses Using The Agilent Bootp Service

    Status: BootP Request received from hardware address: 0010835675AC Error: Hardware address not found in BootPTAB: 0010835675AC Status: BootP Request finished processing at outermost layer 3 Record the hardware (MAC) address (for example, 0010835675AC). Agilent 1200 Infinity Series DAD User Manual...
  • Page 241 3 Click Edit BootP Addresses... The Edit BootP Addresses screen appears. 4 Click Add... The Add BootP Entry screen appears. Figure 66 Enable BootP logging Agilent 1200 Infinity Series DAD User Manual...
  • Page 242 11 Use the PING utility to verify connectivity by opening a command window and typing: Ping 192.168.254.11 for example. The Tab File is located at C:\Documents and Settings\All Users\Application Data\Agilent\BootP\TabFile Agilent 1200 Infinity Series DAD User Manual...

  • Page 243: Changing The Ip Address Of An Instrument Using The Agilent Bootp Service

    1 Select Start > All Programs > Agilent BootP Service and select Edit BootP Settings. The BootP Settings screen appears. 2 When the BootP Settings screen is first opened, it shows the default settings from installation. Agilent 1200 Infinity Series DAD User Manual...
  • Page 244 1 In the Windows control panel, select Administrative Tools > Services. The Services screen appears, see Figure 67 on page 243. 2 Right-click Agilent BootP Service and select Start. 3 Close the Services and Administrative Tools screens. Agilent 1200 Infinity Series DAD User Manual...

  • Page 245: Manual Configuration

    Therefore, manual configuration can be done at any time. A power cycle is mandatory to make the stored parameters become the active parameters, given that the initialization mode selection switches are allowing Figure 69 Manual Configuration (Principle) Agilent 1200 Infinity Series DAD User Manual...

  • Page 246: With Telnet

    When the connection was established successfully, the module responds with the following: Figure 71 A connection to the module is made 3 Type ? and press enter to see the available commands. Figure 72 Telnet Commands Agilent 1200 Infinity Series DAD User Manual...
  • Page 247 Then press [Enter], where parameter refers to the configuration parameter you are defining, and value refers to the definitions you are assigning to that parameter. Each parameter entry is followed by a carriage return. Agilent 1200 Infinity Series DAD User Manual...
  • Page 248 Initialization mode is Using Stored active TCP/IP settings stored TCP/IP settings in non-volatile memory connected to PC with controller software (e.g. Agilent ChemStation), here not connected Figure 74 Telnet - Change IP settings Agilent 1200 Infinity Series DAD User Manual...
  • Page 249 If the Initialization Mode Switch is changed now to “Using Stored” mode, the instrument N O T E will take the stored settings when the module is re-booted. In the example above it would be 192.168.254.12. Agilent 1200 Infinity Series DAD User Manual...

  • Page 250: With The Instant Pilot (G4208A)

    Instant Pilot - LAN Configuration (Edit mode) 5 Press the Edit button (only visible if not in Edit mode), perform the required changes and press the Done button. 6 Leave the screen by clicking Exit. Agilent 1200 Infinity Series DAD User Manual...

  • Page 251: Pc And Agilent Chemstation Setup

    This procedure describes the change of the TCP/IP settings on your PC to match the module’s default parameters in a local configuration (see Table 23 on page 229). 1 Open the Local Area Connection Properties and select Internet Protocol (TCP/IP). Then click on Properties. Agilent 1200 Infinity Series DAD User Manual...
  • Page 252 LAN Configuration PC and Agilent ChemStation Setup 2 You may enter here the fixed IP address of the module or use the Alternative Configuration. Agilent 1200 Infinity Series DAD User Manual...
  • Page 253 LAN Configuration PC and Agilent ChemStation Setup 3 We will use the direct LAN access via Cross-over LAN cable with the module’s IP address. 4 Click on OK to save the configuration. Agilent 1200 Infinity Series DAD User Manual...

  • Page 254: Agilent Chemstation Setup

    The LAN must be connected to detector due to high data load on communication to Control N O T E Software. 1 Open the ChemStation Configuration Editor. 2 Select from the menu Configure - Instruments. 3 Select Modular 3D LC System. 4 Give the Instrument a name. Agilent 1200 Infinity Series DAD User Manual...
  • Page 255 LAN Configuration PC and Agilent ChemStation Setup 5 Click on OK. 6 Select LC System Access — Access Point and click on Add. Agilent 1200 Infinity Series DAD User Manual...
  • Page 256 9 Save the current configuration via File – Save. 10 Exit the Configuration Editor. 11 Start the Agilent ChemStation. During first startup or when the system configuration has changed, a notification shows up. Agilent 1200 Infinity Series DAD User Manual...
  • Page 257 13 Enter the IP address or the Hostname of the module with the LAN-access. 14 Click on OK. The selected module is shown now in the right window (with serial number). In addition all other modules connected via CAN to the detector are shown as well. Agilent 1200 Infinity Series DAD User Manual...
  • Page 258 Under Connection Settings you may change the IP/Hostname of the module (may require a re-start of the ChemStation). After successful load of the ChemStation, you should see the module(s) as active item in the graphical user interface (GUI). Agilent 1200 Infinity Series DAD User Manual...
  • Page 259 LAN Configuration PC and Agilent ChemStation Setup Figure 77 Screen After Successful Load of ChemStation Agilent 1200 Infinity Series DAD User Manual...
  • Page 260 LAN Configuration PC and Agilent ChemStation Setup Agilent 1200 Infinity Series DAD User Manual...

  • Page 261: Appendix

    Agilent 1200 Infinity Series DAD User Manual Appendix Safety The Waste Electrical and Electronic Equipment Directive Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet This chapter provides addition information on safety, legal and web. Agilent Technologies...

  • Page 262: Safety

    C A U T I O N alerts you to situations that could cause loss of data, or damage of equipment. ➔ Do not proceed beyond a caution until you have fully understood and met the indicated conditions. Agilent 1200 Infinity Series DAD User Manual...
  • Page 263 The operator of this instrument is advised to use the equipment in a manner as specified in this manual. Safety Standards This is a Safety Class I instrument (provided with terminal for protective earthing) and has been manufactured and tested according to international safety standards. Agilent 1200 Infinity Series DAD User Manual...
  • Page 264 Do not operate the instrument in the presence of flammable gases or fumes. Operation of any electrical instrument in such an environment constitutes a definite safety hazard. Do not install substitute parts or make any unauthorized modification to the instrument. Agilent 1200 Infinity Series DAD User Manual...
  • Page 265 When working with solvents, observe appropriate safety procedures (for example, goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet by the solvent vendor, especially when toxic or hazardous solvents are used. Agilent 1200 Infinity Series DAD User Manual...

  • Page 266: The Waste Electrical And Electronic Equipment Directive

    Monitoring and Control Instrumentation product. Do not dispose off in domestic household waste N O T E To return unwanted products, contact your local Agilent office, or see www.agilent.com more information. Agilent 1200 Infinity Series DAD User Manual...

  • Page 267: Radio Interference

    Appendix Radio Interference Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or...

  • Page 268: Sound Emission

    This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) Agilent 1200 Infinity Series DAD User Manual...

  • Page 269: Solvent Information

    THF, dioxane, di-isopropylether) such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides, Solvents containing strong complexing agents (e.g. EDTA), • Mixtures of carbon tetrachloride with 2-propanol or THF. • Agilent 1200 Infinity Series DAD User Manual...

  • Page 270: Agilent Technologies On Internet

    Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Agilent 1200 Infinity Series DAD User Manual...
  • Page 271 ASTM remote environmental conditions RS-232 automatic configuration with Bootp cables D/A converter analog Agilent LabAdvisor bandwidth Instant Pilot dark current cable overview Agilent 1200 Infinity Series DAD User Manual...
  • Page 272 PC setup general error messages at fan assembly general method settings illegal value from sensor on main automatic configuration with board using Bootp leak sensor open GLP features Agilent 1200 Infinity Series DAD User Manual...
  • Page 273 RFID tag operating Altitude photometric accuracy overview operating temperature replacing firmware physical specifications optical Agilent 1200 Infinity Series DAD User Manual...
  • Page 274 209, spectrum settings using setup using timeout ChemStation stack configuration troubleshooting Agilent 1200 Infinity Series DAD User Manual...
  • Page 275 Agilent 1200 Infinity Series DAD User Manual...
  • Page 276 (G4212B). • introduction and specifications, • installation, • using and optimizing, • troubleshooting and diagnose, • maintenance, • parts identification, • safety and related information. © Agilent Technologies 2010-2011, 2012, 2013 Printed in Germany 02/2013 *G4212-90013* *G4212-90013* G4212-90013 Agilent Technologies...

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