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Adjusting The Köhler Illumination - Nikon TE2000 Manual

Fluorescent microscope
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Adjusting the Köhler illumination
-this will increase image contrast-
C
1.
Decrease the size of the diaphragm (A) so that you can see the
edges.
2.
Bring the edges of the field iris onto focus by raising or lowering
the condensor focusing knob (B).
3.
Senter the image of the field iris by using the condensor sentering
knobs (C).
4.
Open the diaphragm so the edges lie just beyond the field of
view.
A
B
C
1.
2.
3.
4.
or

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