Adjustment For The Diascopic Illumination (Focusing And Centering The Condenser And Adjusting The Field Diaphragm And Aperture Diaphragm) - Nikon Eclipse LV100DA-U Instructions Manual

13
Adjustment for the Diascopic Illumination
(Focusing and Centering the Condenser and
Adjusting the Field Diaphragm and Aperture Diaphragm)
Focusing and centering the condenser
When this microscope is used for the first time or after the condenser lens is replaced, focus and
center the condenser so that the light through the condenser is focused on the correct position of the
specimen surface (at the center of the optical path).
1 Follow the procedure described in Pages 26 and
27 for the bright-field microscopy under the
diascopic illumination, and focus on the
specimen with the 10x objective.
2 Turn the field diaphragm control on the
microscope base to reduce the opening of the
field diaphragm.
3 Turn the condenser focus knob to form the field
diaphragm image on the specimen surface.
4 Turn the condenser centering screws on both
sides so that the field diaphragm image is
positioned in the center of the field of view.
5 Locate the 50x objective into the optical path.
Turn the fine focus knob to focus on the specimen.
6 Turn the condenser focus knob to form the field diaphragm image on the specimen surface.
7 Adjust the field diaphragm control and the condenser centering screws so that the field
diaphragm image inscribes the field of view.
8 To observe the specimen, turn the field diaphragm control so that the field diaphragm image
circumscribes the field of view. (Adjust the diaphragm image every time when objectives are
changed.)
Image of the field diaphragm
Field of view of the eyepiece
III. Operation of Each Part
Condenser
Condenser
focus knob centering screws
0. 9
= 0. 2
Ach r 0.3
0.4
0.7 0.6 0.5
0.8
EP I
EP I
OB J.
DIA
CU BE
A.S .
DIA
Field diaphragm
control
F .S.
53