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Troubleshooting
1. If NIS elements or the microscope stops working or crashes, try to restart the software.
2. If this doesn't solve the problem, restart all microscope components and the computer.
3. If you are still having difficulties, notify a member of NIS staff immediately. In the event that you cannot find
a member of staff you should send an email describing the problem or use the incident reporting system in
PPMS.
Switching off and tidying.
1. Check the PPMS booking system and if another user is booked within the next hour, leave all the components
on (except for the CO2, if you have used it) and just exit the software.
2. Remember to transfer your data from the datadrive to livecelldata.
3. Remove your sample from the microscope stage.
4. Lower the objective fully using the manual focus wheel and move the stage to the central position.
5. If no user is due on the system after you shut down all components in reverse order to the system start up.
6. Clean oil objectives with the provided lens tissue and ethanol (if you have used oil)
7. Return any oil bottle or slide cleaner used to the image analysis room (shelf above the safe)
8. Dispose of any used tissue in the bins, slides in the sharps bin and clinical waste in the yellow bin in the TC
room.
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Nikon Imaging Centre @ King's College London
Nikon
King's College London
Imaging Centre
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