Chapter 5 Troubleshooting; Problems With The Microscope Image; Common Problems For Each Microscopy - Nikon ECLIPSE Si Instructions Manual

Chapter 5 Troubleshooting
5
Troubleshooting
Misuse of this product may adversely affect performance, even if this product is properly functional. If any of
the following problems occurs, be sure to check the following table for possible causes before requesting
service.
If you detect problems that are not listed below or the problem still persists after measures are taken, unplug
the power cord of the device and contact your nearest Nikon representative.
5.1

Problems With the Microscope Image

5.1.1

Common Problems for Each Microscopy

Dirt or dust in the field of view
Problems
The specimen is dirty if dirt or dust moves when the
specimen is moved on the stage.
Objective is dirty if dirt or dust disappears when the
objective is switched.
The eyepiece is dirty if dirt or dust does not move
when the stage or objective is moved.
Aperture diaphragm is closed too far.
Poor image quality, low resolution, or contrast too low or too high
Problems
No coverglass is attached to the slide.
Coverglass is too thick or too thin.
Slide is upside down.
Lenses or specimen are dirty or dusty.
Aperture diaphragm is opened or closed too far.
Field diaphragm is opened or closed too far.
No immersion oil is applied to the tip of the
oil-immersion objective.
Nikon immersion oil is not used for oil-immersion
observation.
Air bubbles in immersion oil.
Immersion oil adhering to the tip of the dry type
objective.
No coverglass is attached to the slide for observation.
Image too bright
Problems
Setting of the brightness control knob

Chapter 5 Troubleshooting

Check Items
Clean the specimen.

Clean the objective.

Clean the eyepiece.

Open properly. (→ Section 3.5)

Check Items
Attach a coverglass 0.17 mm thick.

Use a coverglass of the specified thickness (0.17 mm).

Turn over the slide so that the cove glass faces up.

Check dirty or dusty parts, and then clean them as

appropriate by reference to the problems described in
"Dirt or dust in the field of view."
Close or open properly.

Close or open properly.

Apply Nikon immersion oil to the objective.

Apply Nikon immersion oil to the objective.

Remove bubbles.

Clean the lens.

Attach a coverglass.

Check Items
Adjust the brightness by rotating the brightness control

knob.
111
Reference
3.3.1
6.1
6.1
3.8
Reference
―
―
3.3.1
―
3.8
3.9
3.4.2
3.4.2
3.4.2
6.1
―
Reference
3.2.1