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SECTION 9,
continued
9.2.2 Tips for Color Measurement
9.2.3 Using Transmittance Units (Transmittance 100% Procedure)
9.2.4 Using Absorbance Units (Absorbance Zero Procedure)
•
Reestablish the analytical zero reference point (re-zero) for the best possible
accuracy and reproducibility. Repeat this procedure when a measurement is
not taken for several hours.
•
Best accuracy and reproducibility are achieved using a Flow-Cell Kit (see
SECTION 5 on page 49). A Flow-Cell system is required to meet the stated
instrument specifications. If a Flow Cell is not used, match sample cells using
the %T or Absorbance modes and the procedure described in Section 2.3.4.2
on page 22).
•
Color, %T and Absorbance measurements use the same "Zero" reference
point. The Absorbance, %Transmittance and Color Unit measurement modes
are re-zeroed simultaneously. Color, Absorbance and %Transmittance can be
measured on a single sample after establishing a zero reference in any of the
three modes.
A blank solution (100% transmittance) must be read before the transmittance
reading mode can be used. This normally is DI water. After inserting an
appropriate color filter in the instrument, transmittance is set to 100% by the
following procedure:
1. Select transmittance by pressing the
shows "%T."
2. Press the
CAL/ZERO
3. The main display flashes
blank solution, close the cell cover, and press
display counts down from 30 to 0 while the blank is being read. The display
reads 100 %T after time expires.
4. Install a sample cell filled with the sample to be measured.
The %Transmittance result is shown on the large display.
A blank solution (zero absorbance) must be read before the absorbance reading
mode can be used. This normally is DI water. After inserting an appropriate color
filter in the instrument, absorbance is zeroed by the following procedure.
1. Select absorbance by pressing the
shows
.
A
2. Press the
key to select the zero.
CAL/ZERO
3. The main display flashes
blank solution, close the cell cover, and press
The display counts down from 30 to 0 while the standard is being read. The
display reads
0.000 A
4. Install a sample cell filled with the sample to be measured. The absorbance
result is shown on the large display.
83
UNITS/EXIT
key to select 100 %T.
, and the mode display reads
100%
UNITS/EXIT
, and the mode display reads
ZERO
after time expires.
key until the units display
. Insert the
00
to read the blank. The
ENTER
key until the units display
. Insert the
00
to read the blank.
ENTER
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