Become An Expert; Hints For Successful Use Of The Capillaries - Agilent Technologies 1100 Series User Manual

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Become an expert

Hints for Successful Use of the Capillaries

• When connecting the capillary to a fitting or the column push the capillary
• When configuring and installing FS tubing, be careful to avoid shutting
• When installing for the first time, or tubing replacement, clean both fitting
• Tighten fittings until snug, but not overtight so as to cause the fused silica
• Should fused silica tubing/fitting be suspected of leaking, set column flow
• Inspect fused silica capillaries under a microscope. If at the surface it looks
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into the fitting firmly and smoothly to avoid gaps. Incorrect setting will
result in sample dispersion which can cause peak dispersion and reduce
the chromatographic fidelity.
doors or inserting cover panels onto the interconnecting FS tubing. Should
a door or panel cause a kink or sharp bend in the tubing, set the pump to
zero, remove the tubing at once and replace to avoid distributing silica
particles through-out the flow circuit.
and fused silica tube with isopropanol or acetone in small amount to
remove particles before connecting to fittings.
capillaries to be crushed. When bending the fused silica tubing, do not bend
or coil into a diameter smaller than 40 mm, to avoid breaking the tubing.
40 mm
to zero, loosen the fitting, reinsert the fused silica tube and retighten the
fitting. Tighten the fitting without re-seating the fused silica tube may allow
a gap to remain between the fused silica and the fitting, resulting in peak
dispersion and poor chromatographic fidelity. Avoid over-tighten of fittings
to avoid crushing the fused silica tubing.
milky, the capillary must be replaced. Crushed fused silica tubing will
distribute small particles throughout the down stream flow path and
require complete flushing of all fittings and valves from the point of the
damaged tube.
1100 Series Nano Pump User Manual