GE AKTA avant User Manual page 506

9 Reference information
9.12 Delay volumes
Step
3
Note:
Set the delay volume in UNICORN
Follow the instructions to set the delay volume between the UV monitor and the Outlet
valve and between the UV monitor and the Fraction collector.
Step
1
2
Note:
506
Action
To obtain the total delay volume, sum up half of the flow cell volume of the
monitor used (that is, the UV or UV/Vis monitor) with all volumes of tubing
and modules that are located after the monitor in the flow path.
For pH-valve V9-pH and V9H-pH always use the volume for the valve in by-
pass position. The system automatically adds the volumes for the flow restrictor
and the pH flow cell when if they are part of the system.
Action
In the System Control module, on the System menu, click Settings.
Result: The System Settings dialog box opens.
Click Fraction collection:Delay volumes
•
Enter the delay volume between the UV monitor and the Fraction collec-
•
tor in the Detector-Frac field
Enter the delay volume between the UV monitor and the Outlet valve in
•
the Detector - Outlet valve field
and click OK.
•
The system will use the delay volume appropriate to the configuration used
and ignore other settings (e.g. the value for Detector-Outlet valve will be ig-
nored if you are using a fraction collector). It is however recommended to set
all delay volumes so that the volumes remain correct if you change fractiona-
tion method.
ÄKTA avant User Manual 29035184 AE