GE AKTA avant User Manual page 457

Phase Name
Column CIP
Column Perfor-
mance Test
Column Prepa-
ration
Column Wash
Elution
ÄKTA avant User Manual 29035184 AE
Description
Unit selection for Method base and Flow rate
•
Monitor settings:
•
pH monitor
-
Air sensor alarm settings
-
UV monitor
-
Note:
The first wavelength of U9-M and the fixed wavelength for
U9-L or U9-L 2nd is always turned on. The second and third
wavelengths for UV monitor U9-M can be set on or off.
Settings for Column Logbook
•
Start Protocol
•
Result name and location
•
Note:
Some of these options may not be required by certain methods.
Note:
The Method Settings phase differs for systems that have the Method
settings phase and the User defined phase as the only available
predefined phases.
Cleans the column after purification runs by rinsing the column
with a cleaning solution to remove non-specifically bound proteins.
By adding steps, several cleaning solutions can be used sequen-
tially.
Tests the efficiency of a packed column in terms of height equiva-
lent to a theoretical plate (HETP), and the peak asymmetry factor
(A ).
s
Prepares the column before use by removing the storage solution
and equilibrating the column. By adding steps, several preparation
solutions can be used sequentially.
Washes out unbound sample after sample application or removes
strongly bound proteins after elution.
Elutes the sample from the column. Defines parameters for the
elution and fractionation settings.
9 Reference information
9.6 Predefined methods and phases
457