9.12 Delay Volumes - GE AKTA avant User Manual

9.12 Delay volumes

Introduction
A number of methods, both theoretical and experimental, exist for determining the delay
volume of a system. The easiest and recommended method is to perform a theoretical
determination. Delay volumes for standard configurations are listed in Standard delay
volumes in ÄKTA avant 25 and ÄKTA avant 150, on page 507.
Explanation of delay volume
The delay volume is the volume between the detector and the fraction collector or outlet
that is used.
The following illustration shows and example of the delay volume between the UV/Vis
monitor and the fraction collector.
The time when a peak is detected
by the UV/Vis detector is called T
Theoretical determination of
delay volumes
A theoretical determination is performed as described in the following steps:
Step
1
2
ÄKTA avant User Manual 29035184 AE
UV/Vis
pH
Conductivity
0
Action
Identify all components in the system flow path that contribute to the delay
volume of interest.
Determine the internal volumes of all hardware modules and tubing, see
Section 9.13 Component volumes, on page 509 for information about theoret-
ical module volumes and Section 9.3 Tubing and connectors, on page 432 for
information about tubing lengths and dimensions.
Valve
Delay volume
9 Reference information
9.12 Delay volumes
Fraction collector
The peak reaches the fraction
collector at time T
1
505