GE AKTA avant User Manual page 141

Using two UV monitors
It is possible to use two UV monitors in ÄKTA avant. UV monitor U9-M will be the first UV
monitor and UV monitor U9-L will be the second.
Note:
Note:
System properties
Follow the instruction to update the system properties.
Step
1
2
3
ÄKTA avant User Manual 29035184 AE
NOTICE
UV and conductivity flow cells on the high pressure side. When
placing UV and/or conductivity flow cells on the high pressure side
of the column, the UV flow cell has a maximum pressure limit of 2
MPa (20 bar) and the conductivity flow cell has a maximum pressure
limit of 5 MPa (50 bar).
When using two UV monitors, the signal from the first UV monitor is by default
used for peak fractionation. This can be changed by editing the text instruction
Fraction Collection:Peak fractionation parameters:Signal source and
choosing UV 2nd as Signal source.
When using two UV monitors with different cell lengths to increase the UV
absorption dynamic range, the signal from UV monitor U9-L comes from the
real cell length and has to be calibrated for exact calculations. The signal from
UV monitor U9-M is automatically calibrated to nominal cell length.
Action
Open the system properties Edit dialog box.
In the Component types list, click Monitors and sensors.
Select the UV monitor 2nd (U9-L) check box in the Component selection
list. Then click OK.
4 Optional instrument modules
4.11 Second UV monitor
141