Prepare The Flow Path - GE AKTA avant User Manual

5.2

Prepare the flow path

Introduction
This section describes the preparation of inlet tubing, outlet tubing, waste tubing and
mixer. For an illustration of the standard typical flow path, see Section 2.4 Liquid flow
path, on page 35.
Select Mixer chamber
To obtain a homogeneous buffer composition, it is important to use a Mixer chamber
suitable for the flow rate of the method. The tables below show what Mixer chambers
to use in ÄKTA avant 25 and ÄKTA avant 150 at different flow rates.
For information on how to install a Mixer chamber, refer to Section 7.8.2 Replace the
Mixer, on page 338.
Note:
ÄKTA avant 25
Three different Mixer chambers are delivered with ÄKTA avant 25. Their volumes are: 0.6
ml, 1.4 ml (mounted at delivery) and 5 ml.
Mixer chamber
volume (ml)
0.6
1.4
5
1
ÄKTA avant User Manual 29035184 AE
If the liquids are difficult to mix, use a larger Mixer chamber to achieve optimal
mixing. However, note that a larger Mixer chamber distorts and delays the
gradient.
Flow rate (ml/min),
Binary gradient
0.25 to 5
0.5 to 15
2 to 25
Technically it is possible to run BufferPro and Quaternary gradients below 1 ml/min, but gradient
formation starts to become slow.
CAUTION
Risk of explosion. Do not use Mixer chamber 15 ml with an ÄKTA
avant 25 system configuration. The maximum pressure for Mixer
chamber 15 ml is 5 MPa (50 bar).
5 Operation

5.2 Prepare the flow path

Flow rate (ml/min),
Quaternary and BufferPro
gradients
1
1 to 2
1 to 6
6 to 25
157