Table of Contents
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Table of Contents
Related Manuals for Hoefer TE62
Summarization of Contents
Transfer Electrophoresis Unit
Function and Description
Explains the purpose and components of the TE42 and TE62 units.
Unpacking
Instructions for safely unpacking and inspecting the unit and its components.
Operating Instructions
Prepare the Buffer
Steps for preparing the transfer buffer solution required for operation.
Prepare the Unit
Steps for preparing the electrophoresis unit for operation, including cooling.
Transfer
Typical Transfer Parameters
Provides typical parameters for protein and nucleic acid transfers.
Care and Maintenance
Cleaning
Instructions and precautions for cleaning the unit after use.
Troubleshooting
Incomplete Transfer
Solutions for issues related to incomplete transfer and blank areas on membranes.
Molecules Do Not Migrate Out of Gel
Troubleshooting steps for when molecules fail to migrate out of the gel.
Diffuse Band Patterns
Solutions for problems causing diffuse band patterns during transfer.
Electrotransfer Notes
Electrophoretic Transfer Advantages
Discusses the benefits and efficiencies of electrophoretic transfer.
Instrument Guidelines
Cooling
Importance and recommendations for active cooling during the transfer process.
Power Setting
Guidelines for setting constant current or voltage modes for optimal transfer.
Protein Transfers
Study Summaries
Summarizes research findings related to protein transfer efficiency and optimization.
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