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Summary of Contents for ThermoFisher Scientific Evolution 350
- Page 1 UV Visible Evolution 350 Spectrophotometer User Guide 269-331200 Revision A March 2018...
- Page 2 © 2018 Thermo Fisher Scientific Inc. All rights reserved. Microsoft, Windows and Excel are either trademarks or registered trademarks of Microsoft Corporation in the United States and/or other countries. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
- Page 3 Site Preparation and Safety Before using the system, read the site preparation and safety manual on the documentation media provided. Always follow the safety precautions in that manual and in this document when using the system. Thermo Scientific Evolution 350 Spectrophotometer User Guide...
- Page 4 Lift the black tab at the front of the sample compartment to raise the entire lid. Slide the door in the lid to the back to provide easy access to the sample position to insert or remove a cuvette. Evolution 350 Spectrophotometer User Guide Thermo Scientific...
- Page 5 2. Remove any accessory from the location where you plan to install the new accessory (see the instructions below). Reference position cell holder Pin B Sample position Pin A Pin B Toggle Pin A Finger hole Thermo Scientific Evolution 350 Spectrophotometer User Guide...
- Page 6 Sample connector These accessories are supported in the sample position: • Smart 7-cell rotary changer – Also supports CVC accessory • Smart 8-cell linear changer – Also supports 8-cell Peltier accessory • Smart sipper Evolution 350 Spectrophotometer User Guide Thermo Scientific...
- Page 7 Alignment hole B Alignment hole A Pin B Pin A D-connector (female) c. Press down on the front of the accessory to secure the connection. The software displays a prompt to initialize the accessory. Thermo Scientific Evolution 350 Spectrophotometer User Guide...
- Page 8 These openings are covered by a layer of black foam to exclude light. Slits cut into the foam allow you to create an opening without allowing excess light into the sample compartment. Slits on bottom of panel Evolution 350 Spectrophotometer User Guide Thermo Scientific...
- Page 9 Smart Rotary 7-Cell Changer. It cannot accommodate the Linear 8-Cell Changers or the Smart Sipper. Connections on the Side of the Instrument There are three communications cable connections on the right side panel of the instrument. Thermo Scientific Evolution 350 Spectrophotometer User Guide...
- Page 10 These are available with one click from the help system home screen. The beam direction in the sample compartment is from right to left. The beam direction in the reference compartment is from front to back. Evolution 350 Spectrophotometer User Guide Thermo Scientific...
- Page 11 There is no need to “balance the beams” in an Evolution 350 spectrophotometer. A cuvette in the reference beam is only necessary if: • You expect the matrix (for example, a buffer solution) to change absorbance during the course of the measurement.
- Page 12 Lamp Compartment Access Panel Lamp Compartment Access Panel A mercury lamp accessory is available for the Evolution 350 either as a factory-installed option or as a user (or Field Service Engineer) installed accessory. To access the lamp compartment, turn the locking screw 90 degrees counterclockwise and lift the panel upwards.
- Page 13 The Evolution 350 does not feature a dedicated purge port. Route the tubing for the purge gas into the sample compartment through one of the openings in the baseplate in the well beneath the reference position.
- Page 14 If you wish to monitor live absorbance readings you can do this using the Live Display mode in INSIGHT software. Refer to the INSIGHT software user guide or the Help system within the INSIGHT software for details. Evolution 350 Spectrophotometer User Guide Thermo Scientific...
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