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Sartorius Manuals
Laboratory Equipment
Biostat RM 50
Operating instructions manual
Sartorius Biostat RM 50 Operating Instructions Manual
Bioreactor prepared for biobrain
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Contents
Table of Contents
Troubleshooting
Bookmarks
Table of Contents
Table of Contents
About this Document
Scope
Accompanying Documents
Target Groups
Symbols Used
Warnings
Other Symbols
Safety Instructions
Intended Use
Personnel Qualification
General Safety Instructions
Significance of These Instructions
Proper Working Order of the Device
Safety Equipment
Danger Due to Electrical Power
Unsuitable Power Supply Cables
Danger Due to Gasses
Danger of Fire Due to Pure Oxygen (O )
Danger Due to Carbon Dioxide
Accessories, Consumables, and Spare Parts
Emergency Instructions
Personal Protective Equipment
Danger Due to Leaked Substances
Danger of Scalding Due to Defective Components
Danger of Chemical Burns from Contact with Feed and Culture Media
Danger of Contamination from Contact with Feed and Culture Media
Danger Due to Components under Pressure
Danger of Injury from Escaping Substances
Risk of Death from Energy that Is Unexpectedly Released
Danger Due to Bursting of the Culture Bag
Hot Surfaces
Heating Mats
Moving Parts
Unexpected Startup of the Device
Heavy Weight of the Device
Device Description
Device Overview
Connections
Rear Connections
Load Cell Module Connections
Connections on the Left and Right of the Device
Load Cell Module (Optional)
Culture Bag Holder
Hood for Culture Bag Holder 20 | 50
Aeration Module (Optional)
Heating Mats
Temperature Sensor
Filter Heaters
Display
Safety Equipment
Main Switch
Overheating Protection
Symbols on the Device
Temperature Control
Setup
Acclimatization
Conditions at the Place of Installation
Unpacking the Device
Laboratory Energy Sources
Emergency Shut-Off Equipment
Power Supply
Gas Supply
Getting Started
Installation Kit
Installing the Culture Bag Holder
Culture Bag Holder with Heating Mats
Installing the Temperature Sensors
Temperature Sensor in Connection with Heating Mats
Connecting the Compressed Air and CO
Supply
Connecting to the Power Supply
Switching the Device on and off
Connecting the Device to Decentralized Control Systems (DCS)
Options for Connecting the Device to Other Devices
Options for Connecting to Deltav Systems
Changing the Communication Settings
Connecting the Device to Ethernet
Connecting the Device to the Biostat
RM Control Unit
Connecting the Device to Decentralized Control Systems
Connecting the Device to Profibus Networks
Operation
Process Sequence Overview
Operating the Control Elements
Main Screen" Menu
Overview" Menu
Aeration
Aeration with Air ("GASFLOW 1," "GASFLOW 2" Menu)
Aeration with CO ("CO " Menu)
Rocker Speed, Rocker Angle | "SPEED" and "ANGLE" Menus
Temperature | "TEMP 1" and "TEMP 2" Menus
Tray Position | "POSITION" Menu
Weighing Function | "LOADCELL" Menu
Loadcell Zero" Submenu
Loadcell Tare" Submenu
User Management | "User Level" Menu
Alarms | "Alarm" Menu
Settings | "Settings" Menu
Setting the Time and Date
Displaying Service Information
Setting the Culture Bag Size
Selecting the Control Mode
Setting the Controller Behavior after a Restart
Starting the Timer for the Process Sequence
Preparing the Culture Bag
Installing Culture Bags on the Culture Bag Holder
Installing the Filter Heaters
Connecting the Air Supply
Inflating the Culture Bag
Filling the Culture Bag with the Culture Medium
Setting the Cultivation Parameters and Starting Cultivation
Sampling and Inoculation
Preparing Sampling or Inoculation
Inoculation
Sampling
Completing the Process, Cell Harvesting, and Shutting down the Device
Stopping the Rocker Movement
Emptying the Culture Bag and Cell Harvesting
Switching off the Device
Cleaning and Maintenance
Preliminary Steps
Cleaning
Maintenance
Device Servicing by Operating Personnel
Servicing the Device by Authorized Personnel
Faults
Troubleshooting
Hardware-Related Faults
Contamination Troubleshooting Table
Temperature Control" Troubleshooting Table
Aeration and Ventilation" Troubleshooting Table
Alarm Messages
Decommissioning
Decommissioning the Device
Transport
Transporting the Device
Storage and Shipping
Storage
Returning the Device and Parts
Disposal
Information on Decontamination
Disposing of Device and Parts
Information on Disposal
Technical Data
Dimensions
Weights, Materials
Device Components
Heating Mat
Temperature Sensor
Filter Heater
Loadcell RM 20 | 50
Culture Bag Positioning and Operating Volumes
Approved Culture Bags
Culture Bag Properties
Pressures in the Culture Bag
Laboratory Energy Sources
Mains Connection
Aeration and Ventilation
Measurement and Control Ranges
Rocker Function
Aeration and Ventilation
Temperature Control
Ambient Conditions
Acoustic Emissions
Communication Interfaces
D-LINK 1: Ethernet
D-LINK 2: RS-232 Serial Interface
D-LINK 3: Profibus DP
Data Table
Accessories and Consumables
Accessories
Consumables
Sartorius Service
Conformity Documents
Licensing Information
GNU Licensing
Appendix
Aerating the Cell Culture
Schematic Representation of Aeration
Overpressure Control
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Operating Instructions
Translation of the Original Operating Instructions
Biostat
RM 20 | 50
®
Bioreactor prepared for Biobrain
®
2882500-000-01
Table of
Contents
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Table of Contents
Troubleshooting
Faults
65
Hardware-Related Faults
66
Temperature Control" Troubleshooting Table
67
Aeration and Ventilation" Troubleshooting Table
68
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Summarization of Contents
About This Document
Scope of Operating Instructions
Details the device versions and firmware applicable to these operating instructions.
Accompanying Documentation
Lists other essential documents to consult alongside these operating instructions.
Target User Groups
Identifies the intended audience and their required knowledge for operating the device.
Symbols Used in the Manual
Safety Instructions
Intended Use of the Biostat RM
Specifies the device's designated application and limitations for safe operation.
Personnel Qualification for Operation
Outlines the necessary knowledge and training for users operating the device.
General Safety Instructions for Users
Provides fundamental safety guidelines for operating the device.
Significance of Operating Manuals
Ensuring Proper Device Functionality
Device Safety Equipment
Electrical Power Hazards
Risks of Improper Power Cables
Hazards Associated with Gasses
Using Approved Parts
Emergency Procedures
Required Personal Protective Equipment
Hazards from Leaked Substances
Risks from Pressurized Components
Uncontrolled Energy Release Risks
Danger from Hot Surfaces
Heating Mat Safety
Risks from Moving Parts
Device Startup After Power Loss
Safe Handling of Heavy Device
Device Description
Biostat RM 20 | 50 Overview
Identifies key components and their physical layout on the device.
Device Connections Overview
Load Cell Module Connections
Device Side Connections
Optional Load Cell Module
Culture Bag Holder Function
Hood for Culture Bag Holder
Optional Aeration Module
Heating Mats Functionality
Temperature Sensor Installation
Filter Heater Purpose
Device Display Features
Device Safety Features
On-Device Safety Symbols
Temperature Control System
Setup and Installation
Device Acclimatization Procedure
Instructions for allowing the device to adjust to ambient conditions before use.
Installation Site Requirements
Lists conditions for the installation location, including space and environment.
Unpacking the Biostat RM Device
Laboratory Energy Source Requirements
Gas Supply Connections and Safety
Getting Started
Installation Kit Contents
Lists the required connections and accessories included with the device.
Installing the Culture Bag Holder
Step-by-step guide for fitting the culture bag holder and heating mats.
Installing Temperature Sensors
Connecting Compressed Air and CO2
Connecting to Power Supply
Switching the Device On and Off
Connecting to Control Systems (DCS)
Connecting to DeltaV Systems
Configuring Communication Settings
Ethernet Connection
Connecting to Biostat RM Control Unit
Connecting to Control Systems (DCS)
Profibus Network Integration
Operation
Process Sequence Overview
Outlines the steps involved in operating the device from setup to shutdown.
Operating the Control Elements
Introduces the user interface and control elements of the device.
Main Screen Menu Navigation
Overview Menu Display
Aeration with Air Supply
Aeration with CO2 Supply
Rocker Speed and Angle Control
Temperature Control Settings
Tray Positioning for Sampling
Weighing Function Operation
Loadcell Zero Adjustment
Loadcell Tare Function
User Management and Levels
Alarm and Message Handling
Device Settings Menu
Service Information Display
Setting Culture Bag Size
Selecting the Control Mode
Controller Behavior After Restart
Starting the Process Sequence Timer
Preparing for Cultivation
Installing Culture Bags
Step-by-step guide for placing culture bags in the holder securely.
Installing Filter Heaters
Instructions for connecting filter heaters to exhaust filters for proper function.
Connecting Air Supply
Inflating the Culture Bag
Filling Culture Bag with Medium
Setting Cultivation Parameters
Cultivation Procedures
Sampling and Inoculation Preparation
Steps to prepare for sampling or inoculating the culture safely.
Inoculation Process
Sampling the Culture
Stopping Rocker Movement
Emptying Bag and Cell Harvesting
Switching Off the Device
Cleaning and Maintenance
Safety Precautions for Cleaning
Highlights electrical, mechanical, and contamination hazards during cleaning.
Preliminary Steps for Cleaning
Cleaning the Rocker Housing
Cleaning Holder and Hood
Maintenance Tasks
Faults and Troubleshooting
General Troubleshooting Steps
Provides a systematic approach to diagnosing and resolving device faults.
Troubleshooting Contamination Issues
Troubleshooting Temperature Control
Troubleshooting Aeration and Ventilation
Understanding Alarm Messages
Decommissioning and Transport
Device Decommissioning Procedure
Steps to safely prepare the device for removal or storage.
Transporting the Biostat RM Device
Guidelines for safely lifting and moving the device.
Storage and Shipping
Device Storage Guidelines
Instructions for storing the device under appropriate ambient conditions.
Returning Devices and Parts
Procedures for returning defective devices or parts to Sartorius.
Disposal Procedures
Information on Decontamination
Guidance on decontamination requirements before device disposal.
Disposing of Device and Parts
Procedures for proper disposal of the device and its components.
Technical Data
Device Dimensions and Specifications
Provides physical dimensions for the device and its accessories.
Device Weights and Materials
Lists weights and construction materials for the device components.
Device Component Specifications
Culture Bag Positioning and Volumes
Approved Culture Bag Types
Culture Bag Material and Sterilization
Culture Bag Pressure Limits
Mains Connection Specifications
Aeration and Ventilation Parameters
Rocker Function Measurement Ranges
Aeration and Ventilation Ranges
Temperature Control Ranges
Ambient Condition Requirements
Acoustic Emissions Levels
Ethernet Communication Interface
RS-232 Serial Interface (D-LINK 2)
Modbus RTU Communication
MFCS (Omron Host Link) Communication
Profibus DP Interface (D-LINK 3)
Data Table for System Parameters
Process Value Data Table
Error Status and Setpoint Data
Commands and Error Acknowledgment
Accessories and Consumables
Available Accessories
Lists accessories that can be ordered for the device.
Consumables for Biostat RM
Lists culture bags and other consumables available for purchase.
Support and Licensing
Sartorius Service Contact
Information on how to contact Sartorius Service for support.
Conformity Documents Overview
States that attached documents confirm device compliance with directives.
GNU Licensing Information
Details licensing terms for included software code.
Appendix
Aerating the Cell Culture
Schematic diagram illustrating the aeration process.
Overpressure Control Mechanism
Table of Contents
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