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Magus POL 890 User Manual

Polarizing microscope
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M AG US POL 8 9 0
PO LAR I Z IN G M IC RO SC O P E
U S E R M A N U A L

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Summary of Contents for Magus POL 890

  • Page 1 M AG US POL 8 9 0 PO LAR I Z IN G M IC RO SC O P E U S E R M A N U A L...
  • Page 2 928 E 124th Ave. Ste D, Tampa, FL 33612, USA +1 813 468-3001 contact_us@levenhuk.com Levenhuk Optics s.r.o. (Europe) V Chotejně 700/7, 102 00 Prague 102, Czech Republic +420 737 004-919 sales-info@levenhuk.cz Magus® is a registered trademark of Levenhuk, Inc. © 2006–2024 Levenhuk, Inc. All rights reserved. www.levenhuk.com 20241217...

  • Page 3: Safety Precautions

    Before using the microscope, please read this user manual carefully to study the instrument design, operation modes and procedures, operational limitations, and safety precautions. Due to the continuous improvements in the microscope design, this manual may not reflect minor design changes that do not affect the microscope performance and operation procedures.
  • Page 4 Bulb installation: – Do not touch the glass surface of the bulb with your bare hands. When installing the bulb, wear gloves or wrap the bulb with a cotton cloth. – Use a clean cotton cloth moistened with alcohol-based disinfectant to wipe dirt off the surface of the bulb.

  • Page 5: Table Of Contents

    Using a calibration slide with a camera 6 TROUBLESHOOTING 7 SCOPE OF DELIVERY 8 CARE AND MAINTENANCE Replacing the fuse in the stand Replacing the halogen bulb of the transmitted light illuminator Replacing the halogen bulb of the reflected light illuminator Maintenance 9 MAGUS WARRANTY...

  • Page 6: Description

    MAGUS Pol 890 Polarizing Microscope has been designed and tested in accordance with the international safety standards. If properly used, the microscope is safe for the customer’s health, life, property, and the environment. Proper maintenance of the microscope is a prerequisite for its reliable and safe operation.
  • Page 7 Ø190mm, 360° rotatable; centerable, fixable, 1° graduation of the rotation angle vernier scale for measuring angles with an accuracy of 0.1°; 45° click-stop Stage mechanical stage attachment moving range: 30×30mm Coaxial coarse & fine focusing knobs on both sides Focusing range: 35mm Coarse focusing travel: 18.85mm/circle Focusing mechanism Fine focusing scale value: 1μm...

  • Page 8: Microscope Kit

    M ICROS COPE K IT The microscope kit includes the following main components: – stand with built-in power source, focusing mechanism, and eco-mode function – transmitted light lamphouse (with halogen bulb or LED) – reflected light illuminator with turret – reflected light lamphouse (with halogen bulb or LED) –...
  • Page 9 Fig. 1. MAGUS Pol 890 Polarizing Microscope. View from the right 1. Diopter adjustment ring 10. Front lens control knob 19. Condenser centering screw 2. Eyepieces 11. Transmitted light 20. Condenser locking screw polarizer rotation ring 3. Mechanism for adjusting the 21.
  • Page 10 Fig. 2. MAGUS Pol 890 Polarizing Microscope. View from the left 1. Interpupillary distance 10. Stage rotation locking screw 19. Pins of transmitted adjustment scale light color filters 11. Condenser 2. Eyecups 20. Transmitted light lamphouse 12. Screw for locking the rotation 3.
  • Page 11 Fig. 3. MAGUS Pol 890 Polarizing Microscope. Rear view 1. Allen wrench 2. ON/OFF switch 3. Power cord connector 4. Fuse block 5. Power connector for transmitted light illuminator 6. Power connector for reflected light illuminator...

  • Page 12: Components

    COMPONENTS STAND The stand 23 (Fig.2) is a one-piece structure with the base. The base has Y-shaped stable ergonomic design. There is a revolving nosepiece 8 (Fig. 2) with objectives 9 (Fig. 2) and a collector in a frame with field diaphragm 12 (Fig.

  • Page 13: Microscope Head

    M ICROS COPE H EA D The microscope head 27 (Fig. 2) is trinocular (binocular with a trinocular tube). The head provides the visual observation of the specimen image. It is installed in the mounting hole of the intermediate attachment 5 (Fig. 1) and secured with the screw. When installing the microscope head, turn the eyepieces towards the stage.

  • Page 14: Condenser

    Each objective has the following inscriptions: "Plan S-Apo" or "Plan Apo" correction type, linear magnification, numerical aperture, "∞" tube length, "0" or "–" coverslip thickness, magnification color code according to the international standard. Objectives with the "∞/0" inscription may be used with specimens without coverslips. Objectives with the "∞/-"...

  • Page 15: Stage

    The iris aperture diaphragm is adjusted (opened/closed) by the ring 3 (Fig. 4). For best image quality, the aperture diaphragm of the brightfield condenser should be closed to approximately 1/3 of the objective exit pupil diameter. To do this, align the mark on the condenser ring with the aperture number of the objective introduced in the optical path. The aperture marking is engraved on the condenser.

  • Page 16: Transmitted Light Illumination

    T RANS M ITTED LI GHT I LLUM INATO R The microscope’s illumination system is essential for obtaining a contrast evenly illuminated image. The transmitted light illumination system includes a transmitted light lamphouse 20 (Fig. 2) with a bulb, a filter system 19 (Fig. 2), a collector in a frame 12 (Fig.
  • Page 17 The lever 3 (Fig. 6) opens the field diaphragm based on the field of view of the objective being used. The field diaphragm limits the image field in the optical system. Excessive opening of the diaphragm illuminates unwanted details of the observed specimen.

  • Page 18: Intermediate Attachment

    IN TE RME DI ATE AT TACH M EN T The intermediate attachment is installed in the slot on top of the reflected light illuminator and secured with a screw on the right using an Allen wrench. A cover is installed in the intermediate attachment during transportation. The general view of the intermediate attachment is given in Fig.

  • Page 19: Unpacking And Assembling

    A quarter-wave plate (λ/4) introduces a relative phase shift of 90° between the orthogonal wavefronts (ordinary and exceptional) when linearly polarized light passes through. It converts linearly polarized light into elliptical or circularly polarized light. The λ/4 plate is used to enhance the contrast of objects with weak birefringence, to determine the birefringence sign in conoscopy, to allow for the quality analysis of conoscopic and orthoscopic images, and to evaluate optical path differences in birefringent samples.
  • Page 20 1. Rotate the coarse focusing knob so that the dovetail of the focusing mechanism is in the upper position and its end part is revealed, as shown in Fig. 10.1. Mount the stage holder with a condenser mount as shown in Fig.
  • Page 21 3. Installing the mechanical attachment on the stage. Insert the two positioning pins on the bottom of the stage attachment into the two positioning holes 3 on the stage as shown in Fig. 10.5. Tighten the screw using a universal Allen wrench. Fig.
  • Page 22 6. Installing the microscope head. Insert the microscope head into the dovetail mount of the intermediate attachment as shown in Fig. 10.8. Tighten the screw V using a universal Allen wrench. While installing the microscope head, always hold it with one hand to prevent it from falling and breaking.
  • Page 23 10. Installing the transmitted light lamphouse. Install the lamphouse at the back of the stand, as shown in Fig. 10.11. Tighten the screw VII using a universal Allen wrench. Fig. 10.11 11. Installing the reflected light lamphouse. Install the lamphouse on the reflected light illuminator, as shown in Fig.
  • Page 24 14. Connect the power cable of the transmitted light illuminator to the right connector on the back of the stand marked with the icon Connect the power cable of the reflected light illuminator to the left connector on the back of the stand marked with the icon When connecting the cables, pay attention to red dots on the plug and socket.

  • Page 25: Transmitted Light Observations

    TRANSMITTED LIGHT OBSERVATIONS SWITCHING ON T H E I LLU MI N ATI O N Before switching on the ON/OFF switch, make sure that the input voltage of the microscope power supply matches the local mains voltage. If not, do not switch on the microscope. Improper input voltage may result in a short circuit or fire. Make sure that the power cord is plugged into the connector on the back of the microscope stand.

  • Page 26: Position Of The Beam Splitter Lever In The Microscope Head

    POSITION OF THE BEAM SPLITTER LEVER IN THE MICROSCOPE HEAD Use lever 1 to select the desired position of the light path. The splitting ratio is shown in Figures 2 on either side of the microscope head. Fig. 14 Light path splitting ratio,% Designation in figure Process eyepieces/trinocular tube...

  • Page 27: Adjusting The Eyepiece Tubes

    The fine focusing knobs are secured on both sides by powerful magnets. The flat and volumetric knobs may be interchanged as necessary. As shown by the arrow in Fig. 16, press one end of the knob until the other end is lifted. Then remove the knob carefully.

  • Page 28: Setting Up Köhler Illumination In Transmitted Light

    The eyepiece tubes may be lowered/lifted by 35° to change the eyepoint height by 100mm for the users of different heights. Fig. 19. Changing the eyepoint height for the users of different heights SE TTI N G U P KÖ H L ER IL LU MI NAT IO N I N T R A N S M IT T ED L I G HT In the light optical microscope, the image quality depends equally on the optics and on the illumination system, so adjusting the illumination is an important preparatory step.

  • Page 29: Centering The Stage And Objectives

    – Remove the eyepiece from the tube and, while observing the objective exit pupil, increase the opening of the aperture diaphragm to 2/3 of the objective exit pupil. This value will be slightly less than the objective aperture. Insert the eyepiece into the tube.

  • Page 30: Transmitted Light Observations With A Polarizer

    Find a target point in the observed image of the object, any noticeable detail of small size (for example, a dark point), and, moving the object on the stage using the knobs 5 of the stage attachment, bring it to the center of the crosshairs 1.

  • Page 31: Transmitted Light Observations With A Polarizer And An Analyzer

    TRANSMITTED LIGHT OBSERVATIONS WITH A POLARIZER AND AN ANALYZER You can observe an object with the polarizer and analyzer both crossed and parallel positions of the polarizing devices. Introduce the analyzer into the optical path using the knob 4 (Fig. 2). Use the scale of the analyzer and polarizer to set the desired crossed or parallel position of the polarizing devices.

  • Page 32: Reflected Light Observations

    REF LECTE D LIGH T O B SE RVAT I O NS The reflected light illumination system allows for Köhler illumination with adjustable field and aperture iris diaphragms. 1. Shutter 2. Field diaphragm centering tools 3. Field diaphragm adjustment knob 4.

  • Page 33: Reflected Polarized Light Observations

    REF L ECT ED PO LA RI ZE D LI G H T O B SE RVAT I ON S Introduce the polarizer 25 (Fig. 1) and analyzer 4 (Fig. 2) into the optical path. Turn the wheel of the microscopy technique module turret to the "R1"...

  • Page 34: Phase-Contrast Device

    When using the darkfield technique with the immersion objective having a high aperture, the objective captures not only the light scattered by the specimen particles, but also the direct rays that create a light background and deteriorate the image contrast. Therefore, all unwanted light should be removed from the room, if possible. When finished, remove the immersion oil with a clean cloth or cotton wool.

  • Page 35: Digital Camera

    Using these data to determine the actual linear size of the specimen, you just need to count the number of divisions of the eyepiece scale aligned with the area of the specimen being measured, and multiply this number by the scale division value specified in this table.

  • Page 36: Troubleshooting

    6 TROUBLESHOOTING Potential problems and remedies (Table 4): Problem Cause Remedy ELECT RI CAL C OMPONENTS The ON/OFF switch is off Switch on the ON/OFF switch The fuse has blown Replace the fuse No illumination in the field of view The circuit board connector has poor Have the connector repaired by a qualified contact...

  • Page 37: Scope Of Delivery

    MECH ANI CAL C OMPONENTS When switching from the low The specimen slide is mounted Mount the slide with the specimen magnification objective to the upside down (coverslip) facing up high magnification objective, the The coverslip is too thick Use the coverslip of the standard thickness objective touches the slide The specimen image when The eyepiece tubes of the microscope...

  • Page 38: Accessories And Spare Parts

    Infinity plan apochromatic objective: Plan Apo 50х BD Optional Infinity plan apochromatic objective: Plan Apo 100х BD Optional Differential Interference Contrast (DIC) device Optional Calibration slide Optional MAGUS digital camera Optional MAGUS monitor Optional C-mount camera adapter Optional ACCESSORIES AND SPARE PARTS Locking screws...

  • Page 39: Care And Maintenance

    CARE AND MAINTENANCE REPL ACING T HE FU SE I N TH E STA N D Before replacing the fuse, turn the ON/OFF switch to "0" position (off). Unplug the power cord from the AC outlet and remove it from the connector on the back panel of the stand.

  • Page 40: Maintenance

    6. Periodic inspection: the microscope should be regularly inspected and serviced to maintain its performance. MAGUS WARRANTY MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with terms and conditions of transport, storage, and operation of the product.
  • Page 42 www.magusmicro.com...

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