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Magus LUM D400 User Manual

Fluorescence digital microscope
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M AG US LUM D 4 00
FLU OR ESC EN C E D I G ITA L MI C RO S C OP E
U S E R M A N U A L

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Summary of Contents for Magus LUM D400

  • Page 1 M AG US LUM D 4 00 FLU OR ESC EN C E D I G ITA L MI C RO S C OP E U S E R M A N U A L...
  • Page 2 928 E 124th Ave. Ste D, Tampa, FL 33612, USA +1 813 468-3001 contact_us@levenhuk.com Levenhuk Optics s.r.o. (Europe) V Chotejně 700/7, 102 00 Prague 102, Czech Republic +420 737 004-919 sales-info@levenhuk.cz Magus® is a registered trademark of Levenhuk, Inc. © 2006–2024 Levenhuk, Inc. All rights reserved. www.levenhuk.com 20241031...
  • Page 3 Before using the microscope, please read this user manual carefully to study the instrument design, operation modes and procedures, operational limitations, and safety precautions. Due to the continuous improvements in the microscope design, this manual may not reflect minor design changes that do not affect the microscope performance and operation procedures.
  • Page 4 11. Do not touch the lens surfaces with your fingers. Use a brush and special lens-cleaning solution to keep the lenses clean. 12. Bulb installation: – Do not touch the glass surface of the bulb with your bare hands. When installing the bulb, wear gloves or wrap the bulb with a cotton cloth.
  • Page 5 7 TROUBLESHOOTING 8 SCOPE OF DELIVERY 9 CARE AND MAINTENANCE Replacing the fuse and the halogen bulb in the microscope stand (for the transmitted light illuminator) Replacing the mercury lamp and fuse (for the reflected light illuminator) Maintenance 10 MAGUS WARRANTY...
  • Page 6 MAGUS Lum D400 Fluorescence Digital Microscope has been designed and tested in accordance with the international safety standards. If properly used, the microscope is safe for the customer’s health, life, property, and the environment. Proper maintenance of the microscope is a prerequisite for its reliable and safe operation.
  • Page 7 Coaxial coarse & fine focusing knobs on both sides Focusing range: 21mm Coarse focusing travel: 39.8mm/circle Focusing mechanism Fine focusing scale value: 2μm Coarse focusing lock knob Coarse focusing tension adjusting knob Transmitted light source 12V/30W halogen bulb with adjustable brightness Reflected light source 100W mercury lamp AC voltage 85–265V, 50/60Hz...
  • Page 8 Windows 8/10/11 (32bit and 64 bit), Mac OS X, Linux, up to 2.8GHz Intel Core 2 System requirements or higher, minimum 2GB RAM, USB 3.0 port, CD-ROM, 17” or larger display Software MAGUS View Mount type C-mount Body material metal...
  • Page 9 Fig. 1. MAGUS Lum D400. View from the right 9. Transmitted light brightness 16. Filter slider (shutter/neutral 1. Eyepieces adjustment ring density filter/free slot) 2. Eyepiece tubes 10. Coarse focusing tension 17. Field aperture centering knob of 3. Reflected light illuminator...
  • Page 10 11 10 Fig. 2. MAGUS Lum D400. View from the left 1. Diopter adjustment ring 8. Stand and base 15. Field aperture centering 21. Mercury lamphouse knob of the reflected power cord 2. Revolving nosepiece 9. Condenser locking screw light illuminator 22.
  • Page 11 MICROSCOPE PARTS STAND The stand 8 (Fig.2) is a one-piece structure with the base. The base has Y-shaped stable ergonomic design. Parts attached to the microscope stand: – revolving nosepiece 2 (Fig. 2) with objectives 3 (Fig. 2) – stage 4 (Fig. 2) –...
  • Page 12 The interpupillary distance is adjusted by rotating the eyepiece tubes 2 (Fig. 1) in the range of 48–75mm. The distance between the eyepieces matching the observer's interpupillary distance is marked on the adjustment scale. For convenience, the microscope head is inclined at 30°. Microscope head magnification: 1x.
  • Page 13 The specifications of the objectives (Table 2): Numerical Working Coverslip, Color Objective identification System Magnification aperture distance, mm marking PL 4х/0.10 ∞/– 4х 0.10 19.8 – PL 10х/0.25 ∞/– 10х 0.25 – yellow PL FL 10х/0.35 ∞/– 10х 0.35 – yellow PL 20х/0.40 ∞/0,17 20х...
  • Page 14 REF LECTE D LIGH T IL LU MI NATO R The general view of the reflected light illuminator is given in Fig. 3. Fig. 3. Reflected light illuminator 1. Illuminator body 5. Field aperture centering knob of 9. Mercury lamphouse the reflected light illuminator 2.
  • Page 15 The turret wheel can be locked in one of the five positions corresponding to one of the four filters mounted on the turret: blue (B), green (G), violet (V), and ultraviolet (UV), or the neutral position (0). The marking of each filter corresponds to the color of the excitation light and its symbol is inscribed on the wheel 2 (Fig.
  • Page 16 Fig. 4. Mercury lamphouse 1 . Lamphouse cover locking screw 6. Mercury lamp 2 . Lamphouse cover 7. Lamp installation rings 3, 4. Lamp adjustment knobs 8. Lamp locking screws 5 . Lamphouse power cord Centering the mercury lamp in the optical path is performed by the knobs 3 and 4 (Fig. 4). The knob 4 moves the lamp holder vertically, and the knob 3 moves it horizontally.
  • Page 17 The mercury lamphouse power cord is plugged into the connector of the power supply 7 (Fig. 5). The power supply is connected to the AC power outlet using an AC power cord. The AC power cord is plugged into the connector 4 (Fig. 5). The mercury lamp power supply is turned on by the switch 1 (Fig.
  • Page 18 Fig. 6b. Installing the mercury lamp Attention! For transportation, a plastic rod is installed in place of the mercury lamp to avoid damage. Use the screwdriver to loosen the screws 1. Open the cover 2. Loosen two screws 5 on the lamp socket 6 and remove the plastic rod.
  • Page 19 Fig. 7. Switching on the illumination and adjusting the brightness Do not keep the brightness adjustment ring in the maximum brightness position for a long period. This may shorten the life of the bulb. Before switching off the microscope, reduce the light intensity to the minimum. PL AC IN G THE SP EC IM E N Place the specimen 1 on the stage.
  • Page 20 Fig. 9. Focusing on the specimen ADJ U STI N G THE E YE P I ECE T UB ES Use the diopter adjustment of the left eyepiece tube to compensate for the observer's ametropia. Start by setting the diopter adjustment to the zero. To do this, rotate the ring 1 to adjust the scale "0" to the indicator 2. While looking through the right eyepiece (with your left eye closed), bring the specimen into focus.
  • Page 21 We recommend memorizing your interpupillary distance for future reference. The eyepiece tubes are 360° rotatable to change the eyepoint height for the users of different heights. With an interpupillary distance being 64mm, a 180° rotation changes the eyepoint height by 50mm. SE TTI N G U P KÖ...
  • Page 22 When you switch to the objectives of other magnifications, do not change the height of the condenser, only adjust the opening of the field and aperture diaphragms. While adjusting the illumination, you should keep in mind that changing the size of the field diaphragm only affects the size of the illuminated field.
  • Page 23 USI N G THE CA ME RA The digital camera is equipped with a 2.3MP sensor producing a realistic image in 1920х1200 pixels. The camera is recommended to be used with 40x, 60x* (optional), and 100x objectives. The camera allows capturing more details with low magnification objectives.
  • Page 24 5 FLUORESCENCE OBSERVATIONS Before starting fluorescence observations, adjust the microscope to work in transmitted light using the brightfield technique, as described above. Placing the specimen, focusing on the specimen and adjusting the microscope head for the fluorescence observations are done in the same way as for the transmitted light observations. During fluorescence observations: –...
  • Page 25 Fig. 15. Switching on the reflected light illuminator 3. Using the filter slider The filter slider 9 has three positions: neutral matt density filter, free slot, and shutter. Once inserted in the illuminator slot, the plate is locked in one of the three positions. Use the "shutter" position when you have completed the observations or during a break in order to avoid excessive excitation light exposure and prevent the specimen from fluorescence fading.
  • Page 26 5. Centering the mercury lamp The optical fluorescence system is pre-centered at the factory. Since the mercury lamp is packaged separately, the center of the mercury lamp may deviate from the light path when it is re-installed. You should center the lamp before operation.
  • Page 27 6 USING OPTIONAL EQUIPMENT DAR KF IE LD CO N D E NSE R The optional darkfield condenser is used in the darkfield microscopy technique. This technique is used to obtain the image of unstained transparent weakly absorbing samples and therefore invisible when observed in the bright field. We recommend setting up the darkfield illumination with the oil condenser as follows: –...
  • Page 28 PO L ARI ZE R/A N ALYZ E R S E T The polarization technique requires using the polarizer/analyzer set which consists of an analyzer and a polarizer. 1. Place the analyzer into the slot above the revolving nosepiece. 2. Place the polarizer on the collector. 3.
  • Page 29 U SING THE CAL I B R ATI O N SLI D E WI T H A CA M ER A The calibration slide (stage micrometer) is used to calibrate the image analysis software for measurements in actual units. In the calibration mode, you should capture an image of the micrometer scale with every objective magnification and indicate the known distance.
  • Page 30 The objective is damaged Have the objective repaired by a qualified technician or replaced Inappropriate coverslip thickness Use the specimen with the coverslip of standard thickness (0.17mm) The specimen is mounted upside Place the specimen with the coverslip down facing up There is immersion oil on the front Remove immersion oil from the front lens lens of the dry objective (most often...
  • Page 31 SCOPE OF DELIVERY The scope of delivery (Table 5) Component Note MIC RO S C OP E MAIN COMPONENTS Stand (with the transmitted light illuminator, power source and focusing mechanism built into the base) Reflected light illuminator Mercury lamphouse ICO Infinitive trinocular microscope head Revolving nosepiece Mounted on the stand Stage...
  • Page 32 3A/250V Fuse Installed in the illumination system Installed in the mercury lamphouse 15A/250V Fuse power supply Bottle of immersion oil AC Power cord Mercury lamphouse power cord Installed on the lamphouse Dust cover User manual D IG ITA L CA ME R A Digital camera USB cable Flash drive with drivers and software...
  • Page 33 REPL ACI N G THE ME RCU RY LAM P A N D F U S E ( FO R T HE R EF L ECT E D L I G H T I L LU MI N ATO R) 1.
  • Page 34 7. Periodic inspection: the microscope should be regularly inspected and serviced to maintain its performance. MAGUS WARRANTY MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with terms and conditions of transport, storage, and operation of the product.
  • Page 36 www.magusmicro.com...