Related Manuals for Magus LUM VD500L
Summary of Contents for Magus LUM VD500L
- Page 1 M AG US LUM V D 5 00 L F LU ORESC EN C E I N VERT ED D I G I TA L M I C RO S C OP E U S E R M A N U A L...
- Page 2 928 E 124th Ave. Ste D, Tampa, FL 33612, USA +1 813 468-3001 contact_us@levenhuk.com Levenhuk Optics s.r.o. (Europe) V Chotejně 700/7, 102 00 Prague 102, Czech Republic +420 737 004-919 sales-info@levenhuk.cz Magus® is a registered trademark of Levenhuk, Inc. © 2006–2024 Levenhuk, Inc. All rights reserved. www.levenhuk.com 20241107...
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Page 3: Safety Precautions
Before using the microscope, please read this user manual carefully to study the instrument design, operation modes and procedures, operational limitations, and safety precautions. Due to the continuous improvements in the microscope design, this manual may not reflect minor design changes that do not affect the microscope performance and operation procedures. - Page 4 11. Bulb installation. This microscope employs LED bulbs as a light source. The bulbs should be replaced by the equipment vendor or in a qualified service center. If you replace the LED yourself, the illumination function may be impaired. Camera 1.
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Page 5: Table Of Contents
6 FLUORESCENCE OBSERVATIONS 7 USING OPTIONAL EQUIPMENT Using the eyepiece with a scale Using the calibration slide with a camera 8 TROUBLESHOOTING 9 SCOPE OF DELIVERY 10 CARE AND MAINTENANCE Replacing the bulb Replacing the fuse Maintenance 11 MAGUS WARRANTY... -
Page 6: Description Of The Microscope
MAGUS Lum VD500L Fluorescence Inverted Digital Microscope has been designed and tested in accordance with the international safety standards. If properly used, the microscope is safe for the customer’s health, life, property, and the environment. Proper maintenance of the microscope is a prerequisite for its reliable and safe operation. - Page 7 Optical design Infinity plan achromatic, parfocal distance: 45mm; long focal length PL L 10х/0.25/4.3; PL L 20x/0.40/8.0; PL L 40x/0.60/3.5 (spring-loaded); Objectives phase-contrast: PL L 10x/0.25/4.3 PHP2; PL L 20x/0.40/8.0 PHP2; magnification, x/aperture/ PL L 40x/0.60/3.5 PHP2 (spring-loaded); working distance, mm PL 4x/0.10* Fixed stage Stage size: 227mm×208mm...
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Page 8: Microscope Kit
Windows 8/10/11 (32bit and 64 bit), Mac OS X, Linux, up to 2.8GHz Intel Core 2 System requirements or higher, minimum 2GB RAM, USB 3.0 port, CD-ROM, 17” or larger display Software MAGUS View Mount type C-mount Body material metal DC 5V, from the USB port of the computer;... - Page 9 Fig. 1. MAGUS Lum VD500L. View from the left 1. Eyecups 10. Fluorescence filter assembly 2. Eyepieces 11. Revolving nosepiece 3. Stand 12. Objectives 4. Transmitted/reflected light illuminator switch 13. Stage 5. ON/OFF switch 14. Condenser mount 6. Coarse focusing tension adjusting ring 15.
- Page 10 Fig. 2. MAGUS Lum VD500L. View from the right 1. Interpupillary distance adjustment ring 10. Reflected light illuminator cable 2. Eyepiece tubes 11. Object moving knob 3. Knob for switching the light path to the trinocular tube 12. Dish holder 4.
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Page 11: Microscope Parts
2 MICROSCOPE PARTS STAND The stand has stable ergonomic design. Parts attached to the microscope stand 3 (Fig. 1): – revolving nosepiece 11 (Fig. 1) with objectives – stage 13 (Fig. 1) – condenser mount 14 (Fig. 1) – transmitted light illuminator 15 (Fig. 1) –... -
Page 12: Microscope Head
M ICROS COPE H EA D The trinocular head provides the visual observation of the specimen image. The microscope head is pre-installed on the stand 3 (Fig. 1). The eyepiece tube assembly is inserted into the head socket and secured with a screw. When installing the eyepiece tubes 2 (Fig. -
Page 13: Transmitted Light Illuminator
The 40x objectives have a spring-loaded mount to prevent mechanical damage to the front lens and the object. If objectives are damaged, we recommend repairing them in the service center. The objectives are intended to image the specimens through air. Do not use immersion oil. T RANS M ITTED LI GHT I LLUM INATO R The microscope illuminator allows for setting up Köhler illumination. -
Page 14: Camera
The microscope employs narrow-band color filters. The excitation occurs in a selected wavelength range, and the emission filter allows the narrow spectrum of the specific wavelength to pass through. For example, the "DAPI" position means that the fluorescence filter introduced in the optical path emits monochromatic radiation of the ultraviolet spectrum range of 348-382nm. -
Page 15: Brightfield Observation Procedure
1. Unpack the microscope and check the scope of delivery using Section 8 of the User Manual. 2. Take out the stand 1 and place it on a stable work table, remove protective packaging and dust cover. 3. Take out the eyepiece tube assembly 2, remove the dust cover. Insert the assembly into the socket 3. Move the eyepiece tubes upwards or downwards to adjust them to the observer’s height. -
Page 16: Using The Beam Splitter Lever
Turn the ON/OFF switch 3 to "–" position. Set switch 2 to the "II" position, which corresponds to the transmitted light observations. Adjust the brightness using the ring 1 so that the light brightness is comfortable for your eyes. Do not keep the brightness adjustment ring in the maximum brightness position for a long period. -
Page 17: Adjusting The Diopter
AD JUST I N G T HE D IOPT E R One of the eyepieces has a diopter adjustment 1 to compensate for the difference in vision between the user’s eyes. Place the 40х objective into the optical path. While looking through the eyepiece without diopter adjustment (with the other eye closed), bring the specimen into focus. -
Page 18: Setting Up Köhler Illumination
Fig. 12. Focusing on the specimen When using high magnification objectives, raise the objective all the way up by rotating the coarse focusing knob and enable the coarse focusing lock knob. After that, focus on the specimen using the fine focusing knob. Adjust the coarse focusing tension. -
Page 19: Observing Specimens In Tall Glassware
– If the light spot is offset from the center of the field of view, as shown in Fig. 15а, adjust the position of the aperture diaphragm using the centering screws 3 so that the center of the aperture diaphragm aligns with the center of the field of view, as shown in Fig. -
Page 20: Calculating The Total Magnification
CALCU LATI N G T HE TOTAL M AGN IF I CAT I ON The total magnification is the eyepiece power multiplied by the objective power. For example, if the eyepiece is 10х/22mm, and the objective is 40х/0.60, the total magnification of the microscope is 10 х... -
Page 21: Phase-Contrast Technique
Using the trinocular tube, you can view the image both in the eyepieces and on the screen. Otherwise, it functions in a similar way: – Loosen the attachment screw 1 and remove the dust cap 2. – Connect the camera 4 to the C-mount adapter. –... -
Page 22: Fluorescence Observations
When using other phase-contrast objectives, place the corresponding phase-contrast annulus into the optical path. 2. Mount the specimen on the stage, focus the microscope. 3. Remove the eyepiece from the tube and insert the centering telescope 2 instead. You will see a dark ring 5 and a light ring 6 in the field of view. -
Page 23: Using Optional Equipment
7 USING OPTIONAL EQUIPMENT U SING THE EYEP I ECE W I TH A SCALE The eyepiece with a scale or reticle can be used to make comparative analysis of the linear dimensions of the individual components of an object. The scale is installed in the plane of the field diaphragm of the 10x eyepiece. -
Page 24: Troubleshooting
8 TROUBLESHOOTING Potential problems and remedies (Table 4): Problem Cause Remedy ELECTRI CAL COMP ONENTS The ON/OFF switch is off Switch on the ON/OFF switch The fuse has blown Replace the fuse No illumination in the field Poor electrical contact Check all the connectors. -
Page 25: Mechanical Components
MECHANI CAL C OMPONENTS The image does not remain The coarse focusing tension adjusting Adjust the coarse focusing tension adjusting sharp during observation knob is loosened, causing the stage knob to lower spontaneously The coarse focusing knob is too The coarse tension adjusting knob is Loosen the tension of the coarse focusing tight to rotate overtightened... -
Page 26: Replaceable Parts
REPLACEABLE PARTS Infinity plan achromatic objective: PL 4x/0.10 WD 21.0mm Optional Infinity plan achromatic objective: PL L PHP2 10х/0.25 phase-contrast WD 4.3mm Infinity plan achromatic objective: PL L 10x/0.25 WD 4.3mm Infinity plan achromatic objective: PL L PHP2 20х/0.40 phase-contrast WD 8.0mm Infinity plan achromatic objective: PL L 20х/0.40 WD 8.0mm Infinity plan achromatic objective: PL L PHP2 40x/0.60 phase-contrast... -
Page 27: Care And Maintenance
10 CARE AND MAINTENANCE R E PL AC IN G T HE BULB This microscope employs LED bulbs as a light source. The bulbs should be replaced by the equipment vendor or in a qualified service center. If you replace the LED yourself, the illumination function may be impaired. -
Page 28: Magus Warranty
7. Periodic inspection: the microscope should be regularly inspected and serviced to maintain its performance. MAGUS WARRANTY MAGUS provides a 5-year international warranty from date of purchase (valid for the entire life of the instrument). The Levenhuk company warrants the product to be free from defects in materials and workmanship. The Seller warrants that the MAGUS product you have purchased meets specification requirements, provided that the Buyer complies with terms and conditions of transport, storage, and operation of the product. - Page 30 www.magusmicro.com...
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