EXFO Burleigh PCS-4100 Series Operating Manual
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EXFO Burleigh PCS-4100 Series Operating Manual

EXFO Burleigh PCS-4100 Series Operating Manual

Motorized patch-clamp micromanipulator
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OPERATING MANUAL
PCS-4100 Series
Motorized Patch-Clamp Micromanipulator
11026-M-00 Rev C

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Summary of Contents for EXFO Burleigh PCS-4100 Series

  • Page 1 OPERATING MANUAL PCS-4100 Series Motorized Patch-Clamp Micromanipulator 11026-M-00 Rev C...
  • Page 2 EXFO Photonic Solutions Inc. (EXFO). Information provided by EXFO is believed to be accurate and reliable. However, no responsibility is assumed by EXFO for its use or for any infringements of patents or other rights of third parties that may result from its use.

  • Page 3: Table Of Contents

    CONTENT 1. Introduction ....................... 1-1 Getting Started ....................1-1 Major Components ..................1-1 Mechanisms of Motion..................1-3 Configurations ....................1-3 Glossary of Symbols ..................1-5 Safety Warnings ..................... 1-6 2. Description....................2-1 Control Unit....................2-1 Operator Pendant.................... 2-2 3. Installation and Setup................... 3-1 Unpacking the Unit ..................

  • Page 5: Introduction

    1 Introduction 1. INTRODUCTION Getting Started Thank you for purchasing a Burleigh PCS-4100 series motorized micromanipulator. This manual is intended to provide in-depth information on the operation and use of the Burleigh PCS-4100. Burleigh PCS-4100 series patch-clamp micromanipulators (Figure 1-1) from EXFO Life Sciences &...
  • Page 6 Burleigh PCS-4100 Series Operating Manual 1 Introduction Figure 1-2: Burleigh PCS-4100 micromanipulator (head stage not included) Operator Pendant The operator pendant (Figure 1-3) is a remote control for positioning the motorized vertical axis. It provides the ability to control both the speed and direction of travel as well as to set and return to two memorized locations.

  • Page 7: Mechanisms Of Motion

    The other is mounted to the moving portion of the vertical axis and allows for an angle adjustment of the pipette holder. EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)
  • Page 8 Burleigh PCS-4100 Series Operating Manual 1 Introduction Figure 1-6: Rotary pivot stage with adjustable stop mechanism Life Sciences & Industrial Division (11026-M-00 Rev. C)

  • Page 9: Glossary Of Symbols

    Do not proceed unless you understand and meet the required conditions. Chassis Ground Motor Direction Switch Input/ Output Control Signals Fuses- User Replaceable Safety Ground EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)

  • Page 10: Safety Warnings

    Burleigh PCS-4100 Series Operating Manual 1 Introduction Safety Warnings WARNING Should this equipment be used in a manner not specified by EXFO Life Sciences & Industrial Division, the protection provided by the equipment may be impaired. Always follow the operating instructions as indicated! WARNING Refer servicing to qualified personnel.

  • Page 11: Description

    The Move indicator will be lit only when motor is being commanded to move. CAUTION Never connect or disconnect the cables when the unit is on. This could cause an arc and damage to the control unit. EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)

  • Page 12: Rear Panel

    Operator Pendant Motor A and Motor B Operator Pendants A and B plug into their respective connections on the back of the unit. Operator Pendant Speed Selector Switch Rotate to select between LOW, MEDIUM and MAX ranges. EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)
  • Page 13 The setting is lost upon power off. To move the vertical stage to the value stored in memory, press and quickly release (hold less than two seconds). EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)

  • Page 15: Installation And Setup

    Figure 3-1: Location of first fine-adjust screw 2. Bring the tip of the adjustment screw into contact with the thrust washer (Figure 3-2). bring into contact Figure 3-2: Location of second fine-adjust screw EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)
  • Page 16 BOTH motor direction switches (see Figure 2-1). Store the bracket and the four screws in the tool kit. Figure 3-6: Location of remaining screws EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)
  • Page 17 Figure 3-9: Location of locking pins. 10. Turn off the control unit. Unplug the AC line cord, the operator pendant and the motor to prepare for mounting on the microscope. EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)

  • Page 18: Mounting The Manipulator

    IMPORTANT Be sure there is enough slack in the cables to prevent tension on the stages when the stages move or the manipulator rotates. EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)

  • Page 19: Mounting A Headstage And Pipette

    HEKA EPC-9 headstage. Additional holes may be added for other headstages. A separate mounting plate is available for the mounting of HEKA EPC10. Contact EXFO Life Sciences & Industrial Division for more information 1.

  • Page 21: Operation

    1. Use the operator control to adjust the vertical axes to the center of travel. 2. Use the manual adjusting knob to center the manual stages. 3. Affix the micromanipulator to the mounting surface. EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)

  • Page 22: Setting The Adjustable Stop

    With the headstage pivot stage facing you, the pin should be resting below the brass block. Tighten the visible set screw with the .050” Allen wrench. The other set screw is covered by the brass block. This is normal. EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)

  • Page 23: Operational Hints

    Loose mechanical components also can cause problems. For more information refer to the EXFO Application Note: Pipette Drift Sources and Solutions for Patch Clamp Recording Experiments. Warm up If it is required to maintain stability of 1 µm per hour, be sure that the...

  • Page 25: Maintenance

    3. Drive the vertical stage to the highest position possible to spread the lubricant along the screw and to lubricate the internal mechanism of the motor. Fig 5-1: Lubricating the motor lead screw EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)

  • Page 26: Electronic Maintenance

    To replace the fuse(s): 1. Turn off the unit and disconnect from the power source. 2. Remove the fuse holder by prying up as shown in Figure 5-3. EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)

  • Page 27: Troubleshooting

    Problem: Excess motion of pipette tip during manual adjustment Typical symptoms Excess or erratic motion of pipette tip during manual adjustment. Pipette sensitive to vibration. "Losing" cells after making a seal. Also refer to Drift later in this section. EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)

  • Page 28: Probable Causes

    Motor may be running but end of travel has been reached and motor is in Stall condition. End of travel may have been reached and motor is stuck. Incorrect connection of the motor cables. EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)
  • Page 29 (Max) positions. Verify that the orange Move light becomes lighted when Up or Down is pressed. Listen closely to the motor to hear any noise. If the motor is running, a noise should be heard. EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)

  • Page 31: Specifications

    7 " x 8.5" x 7.5" 12" x 10" x 8.5" Weight 5 lbs. 6 lbs. Power Requirements Voltage and Frequency 100 to 240 V 50/60 Hz, 1.0A Fuse T4A, 250V, 0.25" x 1.25" [5x20mm] EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)

  • Page 32: Electromagnetic Compatibility

    This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference and (2) this device must accept any interference received, including interference that may cause undesired operation. EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)

  • Page 33: Directive Weee/ (2002/96/Ec)

    Das oben gezeigte, durchkreuzte Mülltonnensymbol soll Sie einladen, diese Systeme zu benutzen. • Sollten Sie weitere Informationen über Systeme zur Sammlung, Wiederverwendung und Wiederverwertung haben, so setzen Sie sich bitte mit ihrer zuständigen Müllentsorgung in Verbindung. EXFO Life Sciences & Industrial Division (11026-M-00 Rev. C)

  • Page 35: Warranty

    EXFO Life Sciences & Industrial Division personnel. The liability of EXFO Life Sciences & Industrial Division (except as to title) arising out of supplying of said product, or its use, whether under the foregoing warranty, a claim of negligence, or otherwise, shall not in any case exceed the cost of correcting defects in the products as herein provided.

  • Page 36: Index

    Index Adjustments Maintenance........5-1 Approach axis angle..... 4-1 Electronics ......... 5-2 Setting the adjustable stop ... 4-2 Lubricating manual adjusting screws 5- Approach axis angle......4-1 Configurations ......... 1-3 Lubricating motor shafts ....5-1 Left-hand ........1-3 Replacing the fuse....... 5-2 Right-hand .........
  • Page 38 EXFO Life Sciences & Industrial Division 2260 Argentia Rd Mississauga, Ontario L5N 6H7 Canada Tel: 1.905.821.2600 | Fax: 1.905.821.2055 Toll free: 1.800.668.8752 (USA & Canada) www.exfo-lifesciences.com burleigh@exfo.com EXFO Electro-Optical Engineering Inc 400 Godin Ave Quebec City, Quebec G1M 2K2 Canada Tel: 1.418.683.0211 | Fax: 1.418.683.2170...

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