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Summary of Contents for PicoQuant FluoTime 250
- Page 1 FluoTime 250 Automated, Compact, High Performance Fluorescence Lifetime Spectrometer Maintenance Manual Document version 1.0.2...
- Page 3 PicoQuant GmbH FluoTime 250 Maintenance Manual Table of Contents General safety information........................3 1.1. Warning Symbols and Conventions....................3 1.2. Electrical Safety Instructions......................4 1.3. Laser Safety Instructions......................... 4 1.4. Laser Safety Labels......................... 5 1.5. Remote Interlock Connector......................7 1.6.
- Page 4 1. Setup Manual for the FluoTime 250 The printed Setup Manual describes how to unpack and set up the FluoTime 250. Please keep the Setup Manual in a safe place along with the packaging material in case you need to ship the Fluo- Time 250 somewhere.
- Page 5 PicoQuant GmbH FluoTime 250 Maintenance Manual 1. General safety information CAUTION! Before using this device, make sure that you have read and understood the con- tent of this user manual. Store this documentation in a safe and easily accessible place for future reference.
- Page 6 This device contains electrical components that are not user serviceable. Servicing of these internal electrical components is restricted to qualified personnel. The top cover of the FluoTime 250 can be removed to install / replace filters in the filter wheel. Please refer to section 3.3 of this manual.
- Page 7 PicoQuant GmbH FluoTime 250 Maintenance Manual What does the owner / operator have to observe? The owner / operator of this product is responsible for proper and safe operation and for following all • applicable safety regulations. The owner / operator is fully liable for all consequences resulting from the use of the laser for any •...
- Page 8 LDH laser heads. On the FluoTime 250 Several laser safety labels are affixed to the housing of the FluoTime 250, which are highlighted n red (Fig. 2. Fig. 2: Location of the laser safety labels on the FluoTime 250. The system is labeled in 4 positions and the signs indicate that access to a class 3B / IIIB laser beam when:...
- Page 9 PicoQuant does NOT endorse the use of non-approved third-party laser devices with the FluoTime 250 sys- tem. The end-user should not connect non-approved laser devices to or operate the FluoTime 250 with them. PicoQuant declines any responsibility and cannot be held liable for any direct or indirect damages to the users and/or instrumentation resulting from the use of non-approved third-party laser devices by the end- user.
- Page 10 FluoTime 250 Maintenance Manual 2. System Components 2.1. Instrument Overview Fig. 3: Front (top) and back (bottom) view of the FluoTime 250 spectrometer. Note that in its basic configuration the FluoTime 250 is equipped with a PMA detector. Page 8...
- Page 11 Emission polarizer slider Detector assembly Pre-amplifier terminal (optional component) Identification and product labels (including PicoQuant logo, model name, serial number, WEEE and CE symbols). Note that model name and serial number need to be included for any support requests. Grounding pin (refer to the Setup Manual for instructions)
- Page 12 Excitation polarizer (optional component) Attenuator / excitation shutter Excitation lens Detection lens Motorized emission filter wheel. Refer to section 3.3 on how to exchange filters. Variable emission attenuator Emission polarizer Fig. 4: Inside view of the FluoTime 250 spectrometer Page 10...
- Page 13 Removing the top cover WARNING! Turn the FluoTime 250 and all of its components (e.g. laser driver or external TCSPC units) OFF before removing the top cover. Start by loosening the 6 thumb screws (highlighted by solid red circles in Fig. 5), no tool is required •...
- Page 14 PicoQuant GmbH FluoTime 250 Maintenance Manual 3.2. Changing an LDH or PLS Head In order to facilitate light source swapping, the LDH or PLS head is attached to the Laser Coupling Port ( Fig. 3). The head is secured to the port with a single screw (highlighted in red Fig. 9).
- Page 15 5. Detach the laser head from the laser coupling port of the FluoTime 250. Start by releasing the holding screw and then slide the laser head out. 6. Slide the desired laser head into the laser coupling port of the FluoTime 250 and secure it with the holding screw.
- Page 16 (IRF). The filter wheel comes pre-equipped with one or more long-pass filters whose cut-off wavelengths are suited for the lasers shipped with your FluoTime 250. Spectral characteristics of the included filters are provided in this manual’s Appendix.
- Page 17 Use the Filter Configuration dialog to set up the EasyTau2 so that the desired wheel position can be selected by its descriptive name. This procedure is described in the EasyTau2 manual. Fig. 11: Filter wheel with fixing screw (red) indicated by a red arrow (left). Location of the filter wheel in the FluoTime 250 (highlighted in green, right).
- Page 18 There might be loose parts present in the sample mounting unit (cuvettes, samples) 5. Insert the new sample mounting unit by aligning it with the FluoTime 250 sample chamber and slide it gently in until the unit locks into place.
- Page 19 PicoQuant GmbH FluoTime 250 Maintenance Manual Fig. 13: Line connectors for dry gas and water tubes with release ring (1) and glands (2). 2. Repeat step one for the second connector. 3. Push one of the tubes gently into the left line connector labeled ‘Water’...
- Page 20 FluoTime 250 Maintenance Manual 3.5. Changing the Detector Unit Your FluoTime 250 was either shipped with a detector unit from PicoQuant’s PMA Series, PMA Hybrid Series or a NIR-PMT from Hamamatsu (H10330-25 or H10330-45). Only detectors of the PMA and PMA Hybrid Series can be changed by the end-user.
- Page 21 9. Attach the protective cap to the entrance of the detector unit removed in step 7. 10. Attach the new detector unit either directly to the FluoTime 250 or the monochromator. Note that all detector units provided by PicoQuant are delivered with an adapter plate (see Fig. 16).
- Page 22 PicoQuant GmbH FluoTime 250 Maintenance Manual 4. Technical Data / Specifications General Specifications Optical configuration......L-geometry Modes of operation......Time-correlated Single Photon Counting (TCSPC) ............Multichannel Scaling (MCS) Lifetime Range PMA series detector / TCSPC.....50 ps to 10 µs PMA-Hybrid series detector / TCSPC..< 20 ps to 10 µs Excitation Sources Diode lasers.........Picosecond pulsed, with repetition rates up to 80 MHz...
- Page 23 PicoQuant GmbH FluoTime 250 Maintenance Manual Bin width..........4 ps Dead time..........< 95 ns Full scale time range......260 ns – 33 µs Operating Environment Computer system........Windows Power requirements......110 to 230 V; 50 / 60 Hz Max. current draw........5 A at 230 V (depending on accessories used) Max.
- Page 24 PicoQuant GmbH FluoTime 250 Maintenance Manual 5. Support 5.1. Returning Products for Repair Should you encounter problems that require sending the device in for inspection / repair, please contact us first at: https://support.picoquant.com support@picoquant.com and request an RMA number before ship- ping the device.
- Page 25 FluoTime 250 Maintenance Manual 6. Legal Terms 6.1. Copyright Copyright of this manual and on-line documentation belongs to PicoQuant GmbH. No parts of it may be reproduced, translated, or transferred to third parties without written permission of PicoQuant 6.2. Trademarks Other products and corporate names appearing in this manual may or may not be registered trademarks or subject to copyrights of their respective owners.
- Page 26 PicoQuant GmbH FluoTime 250 Maintenance Manual This page was intentionally left blank. Page 24...
- Page 27 PicoQuant GmbH FluoTime 250 Maintenance Manual All information given here is reliable to our best knowledge. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications and external appearances are subject to change without notice. PicoQuant GmbH P +49-(0)30-1208820-0...
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