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Laxco SeBa2 User Manual

Upright digital microscope systems
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SeBa2 Upright Digital
Microscope Systems
User's Manual
Version 4.0

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Summary of Contents for Laxco SeBa2

  • Page 1 SeBa2 Upright Digital Microscope Systems User’s Manual Version 4.0...

  • Page 2: Table Of Contents

    Contents 1. Introduction 2. Operation Notice 3. Installation Included Items Initial Installation 4. Instrument Components 5. SeBa Software Touchscreen Interface 6. Operation Basic Operation SeBaAccess Login Capture Image Record Video Output to HDMI Display 7. Advanced Software Settings and Controls WiFi Network Connection Save Settings: Destination Save/Preview Settings: Resolution and File Type...
  • Page 3 Gallery View Captured Images Upload to SeBaShare Return to Live Image SeBaShare Upload SeBaShare Website Initial Login / Account Setup Changing User Name and Password 8. Microscope Controls Illumination Adjustment Placing Slide on Stage Focus Adjustment Focus Tension Adjustment Centering the Condenser Adjusting the Aperture Diaphragm Adjusting the Field Diaphragm Using an Oil Objective (100X)

  • Page 4: Introduction

    Information provided by Laxco Inc. is believed to be accurate and reliable. However, no responsibility is assumed by Laxco Inc. for its use. The information contained in this pub- lication is subject to change without notice. Words that Laxco Inc. considers trademarks have been identified as such.

  • Page 5: Operation Notice

    2. Operation Notice The microscope is a precision instrument, please operate carefully: avoid vibrating or jolting the microscope during operation. 2. Do not operate the microscope under conditions of direct sunlight, high temperature, high humidity, dust, or close to sources of vibration. Ensure the work surface is flat. 3.

  • Page 6: Installation

    SeBa2 Digital Imaging System is shipped with the objectives, condenser and stage pre-installed and aligned requiring only minimal assembly. 1. Remove the SeBa2 from its packaging and place it on a solid flat surface. *NOTE: Keep all packaging 2. Ensure power switch is in the “0” (OFF) position.

  • Page 7: Instrument Components

    On/Off Switch Tablet (Small button on top left) Power On 1. Turn on tablet power switch which is located on the top left of the SeBa2 tablet. 2. Turn on microscope power switch which is located on the right side of the SeBa2 system.
  • Page 8 . Instrument Components Touchscreen Display Objective Turret X/Y Mech. Stage Condenser Field Diaphragm Coarse/Fine Focus Knobs...

  • Page 9: Seba Software Touchscreen Interface

    5. SeBa2 Software Touchscreen Interface Capture Record Live User Effects Measure Gallery Still Video Settings Menu Menu Menu *Note: To return to full screen mode and “hide” the Effect, Mea- sure or Playback menu simply touch the icon which corre-...
  • Page 10 5. SeBa2 Software Touchscreen Interface Expanded Menu Details Measure Menu Gallery Menu Effects Menu Adjust exposure, Perform measurements Review captured im- white balance, color, on image. ages and upload to SeBaShare. brightness, contrast of live image.

  • Page 11: Operation

    Software controls can be operated via touch or mouse. Turn on the power to the SeBa2 Imaging System and allow the SeBa Software Interface to auto boot. 2. Place a microscope slide (coverslip up) into the slide insert on the microscope stage.

  • Page 12: Capture Image

    Output to HDMI Display 1. To display image on an external HDMI monitor/display, plug in HDMI cable to the port located on the back of the SeBa2 display and then plug HDMI cable into monitor. *Note: HDMI cable not supplied with SeBa2 Digital...

  • Page 13: Advanced Software Settings And Controls

    7. Advanced Software Settings and Controls WiFi Network Connection The SeBa2 can connect to a wireless network using the built in WiFi capabilities. This enables the use of the SeBaShare cloud for image storage and sharing capabilities. Refer to the SeBaShare section for additional details.

  • Page 14: Save Settings: Destination

    7. Advanced Software Settings and Controls Save Settings: Destination The SeBa2 can save images to the internal storage or a USB Drive. This setting must be made prior to saving an image to ensure the image is saved in the desired destination.

  • Page 15: Save/Preview Settings: Resolution And File Type

    7. Advanced Software Settings and Controls Save/Preview Settings: Resolution and File Type The SeBa2 can has separate resolution values for the Preview (live) image and save images. Captured images can be saved as .jpg, .png and .tiff. This must be set prior to capturing and image. For a full resolution, non-compressed image .tiff is the recommended file type.

  • Page 16: Image Orientation

    Advanced Software Settings and Controls Image Orientation The Image Orientation is factory set and does not need to be changed unless you want the image displayed in an alternative orientation. 1. Click on the gear icon at the bottom of the screen to open the User Settings window.

  • Page 17: Bluetooth Settings

    7. Advanced Software Settings and Controls Bluetooth Settings The SeBa2 can be connected to a Bluetooth device such as a keyboard an mouse. *Note: This does not permit saving images to a Bluetooth device. 1. Click on the gear icon at the bottom of the screen to open the User Settings window.

  • Page 18: Camera Settings

    7. Advanced Software Settings and Controls Camera Settings 1. Exposure • Auto Exposure: Click box to enable Auto Exposure. • AE Adjustment: When AE is active this slider will increase or decrease the AE target brightness. • Exposure: When in manual exposure mode adjust slider until desired exposure is achieved.

  • Page 19: Restore Defaults

    7. Advanced Software Settings and Controls Camera Settings (Cont.) 6. Restore Defaults • To restore the settings in the Effects window to defaults click the “Restore Defaults” button. • Note: This only applies to WB, Color Temperature, Contrast, Saturation, Sharpness, Gamma, Color Mode, and Power Frequency.

  • Page 20: Measurments

    . Advanced Software Settings and Controls Measurements 1. Calibration The Seba2 will include basic calibrations prior to shipment. To add calibrations or re-calibrate the system follow the steps below. *Note: Requires stage micrometer to complete process. Laxco PN: MP-SM100. •...

  • Page 21: Text Annotation

    . Advanced Software Settings and Controls Calibrated Measurement (Cont.) • The measurement will be placed in the upper left hand corner of the display. • Move the measurement to desired location by clicking and holding the left mouse button or press and hold if using touchscreen. •...

  • Page 22: Measurement/Annotation Settings

    7. Advanced Software Settings and Controls 5. Measurement/Annotation Settings • Select the Settings icon to open up the Annotations Setting menu. *Note: These settings will change all annotations and measurements. • Adjust Line Color, Line Width, Text Color and Text size and select “OK. •...

  • Page 23: Gallery

    7. Advanced Software Settings and Controls Gallery View Captured Images • Select the Gallery icon at the bottom right corner of the screen to open the Gallery menu. • Images that are currently saved in the directory selected in “User Settings” (Pg.16) will be displayed in the Gallery menu.

  • Page 24: Sebashare Upload

    7. Advanced Software Settings and Controls 3. Return to Live Image • When a captured image from the gallery is displayed the “capture” button changes to a “LIVE” button. • Selecting the “LIVE” button return the display to the live image. 4.

  • Page 25: Sebashare Website

    . Advanced Software Settings and Controls SeBaShare Website The SeBa2 Digital Imaging System includes access to the SeBaShare Cloud for file storage and file sharing. To connect the SeBa2 system to your SebaShare cloud refer to the “SeBaAccess Login” section. 1. Initial Login/Account Setup •...

  • Page 26: Changing Username And Password

    . Advanced Software Settings and Controls 2. Changing Username and Password • To access your personal account settings click on the drop down menu in the upper right hand corner of the SeBaShare homepage and choose “Personal”. • On the “Personal” page enter your email address. This can now be used as your new username for logging in to SeBaShare.

  • Page 27: Microscope Controls

    . Microscope Controls Illumination Adjustment 1. Turn on the microscope. 2. Adjust the light adjustment knob (1) to a desirable level . Rotate the light adjustment knob (1) toward the thick side of the indicator to increase brightness, and towards the thinner side of the indicator to reduce brightness.

  • Page 28: Focus Tension Adjustment

    8. Microscope Controls Focus Tension Adjustment 1. If the coarse focusing knob’s tension is too high; or if the stage moves out of focus, adjust the focus tension (1) according to the indicator on the knob. Centering the Condenser 1. Rotate the condenser knob (2) to raise it to the highest position.

  • Page 29: Adjusting The Aperture Diaphragm

    8. Microscope Controls Adjusting the Aperture Diaphragm 1. The aperture diaphragm controls the numerical aperture (NA) of the illumination. A matching NA between the illumination and the objective will provide better resolution, contrast, and depth of field. 2. If your sample has low contrast, it is advised to adjust the condenser aperture diaphragm to be 70%-80% of the N.A.

  • Page 30: Using And Oil Objective (100X)

    8. Microscope Controls Using an Oil Objective (100X) 1. Use the 4X objective to focus the specimen. 2. Place a drop of oil (1) on the specimen 3. Rotate the nosepiece counterclockwise and rotate the oil objective (100X) to the light path.

  • Page 31: Technical Specifications

    9. Technical Specifications Touchscreen Microsoft Touchscreen Display Camera 16MP Color CMOS Camera Connectivity WiFi Bluetooth 4 USB Ports HDMI Seba Software Capture Still images and video Upload images to SeBashare cloud Save to USB Drive Review, measure and annotate images Optical System Color corrected infinity optical system Eyepiece...

  • Page 32: Customer And Technical Service

    10. Customer and Technical Service Returned Goods Policy for Repair or Replacement Parts To return goods for repair or replacement, please contact Laxco Customer Service at 425-686-3081. Please be prepared to supply the following information: •Your name, return shipping address and telephone number •Catalog/Model number of the item(s)you are returning...

  • Page 33: Reference

    11. Reference KEY CONCEPTS MAGNIFICATION Magnification increases the apparent size of an object you are viewing through the micro- scope. Magnification by itself does not provide more information about an object unless there is also adequate resolution and contrast. The objective lens and oculars (eyepieces) determine magnification.

  • Page 34: Glossary Of Microscopy Terms

    12. Glossary of Microscopy Terms Abbe Condenser: A simple condens- C-mount: A type of camera adapter typi- er comprised of two lenses; corrects for cally used to connect video cameras to a chromatic aberration. microscope. Aberration: Term used to describe any CCD: Type of video camera using electron- inaccuracy in focusing of light;...
  • Page 35 12. Glossary of Microscopy Terms Depth of Field: The vertical distance in the Field Curvature: One of the optical dis- sample through which features are simul- tortions. When the center of the image is taneously in focus. High numerical aperture in focus and the edgesthe field is said to objectives have a “shallow depth of field”...
  • Page 36 12. Glossary of Microscopy Terms Image: The focusing of light in an orga- High Eyepoint: A design characteristic of nized fashion to reproduce information eyepieces in which the back focal plane of collected from the object. The more ac- the eyepiece is raised about 18 mm above curately the light is focused, the more the top of the eyepiece to accommodate accurately the object is represented in the...
  • Page 37 12. Glossary of Microscopy Terms nfinity Corrected Optics: A special opti- Light, Polarized: Light in which the waves cal design involving at least two lenses in vibrate in only one direction, perpendicular which the object is placed at the focal plane to the direction of travel. of the first lens, causing the imaging rays to emerge parallel to the optic axis or some Long Working Distance (LWD): A term...
  • Page 38 12. Glossary of Microscopy Terms Microscope, Stereo: A standard microscope Phase Contrast: A contrast-enhancement configuration based on two independent technique that detects phase objects. It imaging paths, separated by approximately uses a special ring, placed in the condenser 10-12 degrees, resulting in a stereoscopic to control location of the undiffracted light, image characterized by great three-di- and a matching phase plate, placed in the...
  • Page 39 12. Glossary of Microscopy Terms Primary Image: The first magnified image formed in the microscope. Trinocular Port: A special eyepiece, usu- ally narrower in design than conventional Primary Image Plane: The location of the eyepieces, used in the photo tube of the first magnified image formed by the objec- microscope to project a real image to the tive.
  • Page 40 LAXCO, Inc. Contact us: 18303 Bothell-Everett Hwy 425-686-3081 Suite 140 Sales@laxcoinc.com Mill Creek, WA 98012 www.laxcoinc.com © 2023 LAXCO Inc. All rights reserved. Laxco™ is a registered trademark of Laxco Inc., in the U.S. and/or other countries. SEBA-MKT-DOC-1700112(4)

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