Table of Contents
Advertisement
Quick Links
Advertisement
Table of Contents
Related Manuals for Laxco SLi6Pro
Summary of Contents for Laxco SLi6Pro
- Page 1 SLi6Pro Inverted Fluorescence Microscope User’s Manual Version 1.0...
-
Page 2: Table Of Contents
CONTENTS 1. I ntroduction 5. Camera Installation 5.1 Installing SeBaCam Disclaimer Safety Conventions 6. Troubleshooting 18, 19 Prior to use Optical System Mechanical System 2. Components Electrical System LED Flourescence Assembly 3. Assembly 7. Care and Maintenance Assembly Precautions Assembly Steps 1. -
Page 3: Introduction
Information provided by Laxco Inc. is believed to be accurate and reliable. However, no responsibility is assumed by Laxco Inc. for its use. The information contained in this publication is subject to change without notice. Words that Laxco Inc. considers trademarks have been identified as such. However, neither the presence nor absence of such identification affects the legal status of any trademark. -
Page 4: Power Cord
Attach the stage extension or mechanical stage as follows: Screw the lock-screws (1) into the bottom of the stage extension or mechanical stage. Screw it into the bottom of the stage. screws 3-2-7 POWER CORD Turn the power switch (1) to the “o” OFF position before connecting the power cord. -
Page 5: Prior To Use
PRIOR TO USE The microscope is a precision instrument. Please operate properly avoiding vibration or sudden jolting the microscope during operation. Do not operate the microscope in direct Microscope sunlight, high temperature, high humidity, dusty environment, or close to sources of vibration. -
Page 6: Maintenance
MAINTENANCE 1. Utilizing a lens tissue moistened with a small amount of lens cleaning solution, gently wipe the eyepieces and condenser removing all oil and fingerprints on the optical surfaces. Lens cleaning solution may be flammable. Turning on or off electronic devices (including the microscope) may produce a spark which could ignite the lens cleaning solution. -
Page 7: Components
2. COMPONENTS LED Lamphouse Aperture Diaphragm Adjusting Slider Eyepieces Camera Port Darkroom Slider Fixed Stage Nosepiece Trinocular Body Coarse & Fine Focus Knobs... -
Page 8: Assembly
ASSEMBLY 3.1 ASSEMBLY PRECAUTIONS Following are the recommended assembly steps with each step numbered denoting the assembling order. Before assembling, make sure there is no dust, dirt or other materials which will effect the operation. Assemble carefully while avoiding contact with any glass surfaces. 3.2 ASSEMBLY STEPS... -
Page 9: Objectiveassembly
3-2-1 OBJECTIVE ASSEMBLY Rotate the coarse focus knob, until the objective nosepiece is at the lowest position. Install the objectives into the microscope nosepiece from the lowest magnification to the highest in a clockwise direction from the back of the microscope. Objectives can also be assembled by removing the metal/glass plate on the stage. -
Page 10: Eyepiece Assembly
3-2-3 EYEPIECE ASSEMBLY Eyepiece Remove the eyetube cover. Insert the eyepiece into the eyetube. 3-2-4 PHASE - CONTRAST SLIDER ASSEMBLY 1. Place phase-contrast slider letter side up, into the holder from the right to the left. Phase-Contrast Slider To change setting, slide the correct phase ring into place. -
Page 11: Color Filter Assembly
3-2-5 COLOR FILTER ASSEMBLY (FOR TRANSMITTED ILLUMINATION) Turn the microscope off and allow the filter to cool before changing. Color Filter Slide out the filter holder and place the color filter in to the filter holder. Ensure that the filter is flat and firmly pressed into the bottom of the filter holder. -
Page 12: Operation
4. OPERATION 4-2 SPECIMEN PLACEMENT illumination slider control Plug the microscope into a power outlet and turn the power switch to the ON position. Press the BF/PH button located at the lower left on the membrane control panel on the front of the microscope body. -
Page 13: Focus Adustment
Operation of the attachable mechanical stage Place the multi-well plate on the mechanical stage holder (4) when using 96 or 24 well plate. Stage Vessel Holders: Holder • Terasaki holder for Terasaki plates. Specimen Slide • φ35mm vessel holder for φ35mm dish. -
Page 14: Focus Tension Adjustment
4-4 FOCUS TENSION ADJUSTMENT If the coarse focus knob is difficult to rotate or the objective nosepiece “drifts” or loses focus this can typically be corrected by adjusting the focus tension. Rotate the tension adjustment ring according to the arrow direction in the figure to tighten the focus tension;... -
Page 15: Interpupillary Distance Adjustment
4-6 INTERPUPILLARY DISTANCE ADJUSTMENT 1. While looking through both eyepieces, move the eyepieces together or apart until the field appears as one circle and viewing is comfortable. 2. The number on the index (2) that lines up with the “.” On the side (1) is the inter-pupillary distance of your eyes. -
Page 16: Color Filter
4-8 USE THE EYE-CAP 1. If the user wears glasses, turn the eyecup downward to prevent the glasses from touching the eyepiece and avoid damage to the glasses and the eyepiece. 2. Raise the eyecup for users without glasses. In this mode, the eyecup can prevent unwanted outside light. -
Page 17: Condenser Lens Removal
4-10 CONDENSER LENS REMOVAL The condenser lens can be removed to view specimens in large vessels, or cell stacks (up to 5 layers). Unscrew the condenser lens (1) to increase the working distance 4-11 Enhanced Darkfield Contrast Illumination The ring of LEDs for Darkfield Contrast are located below the fixed stage aperture, and provide illumination at an oblique angle to help find a focal plane and region of interest before switching... -
Page 18: Darkroom Light Shield
4-13 DARKROOM LIGHT SHIELD The Darkroom Light Shield may be utilized to block extraneous room light while using the SLI6P in Fluorescence Mode. The shield, located behind the condenser may be lowered and rotated close to the vessel, well plate, or slide to improve the Fluorescence signal to noise ratio. - Page 19 4-15 OPERATING THE FLURESCENCE LEDs ON SLI6P CONTROL PANEL Select the desired LED from the Membrane Touch Panel, and press the button. Once for ON, Once again for OFF. Adjust the intensity using the slider on the right hand side of the panel. Add additional Channels by selecting the proper excitation LED(s) For Multi-Illumination Contrast, the...
-
Page 20: Camera Installation
5. CAMERA INSTALLATION 5.1 CAMERA INSTALLATION Select the appropriate c-mount adapter. (Note: c-mounts and cameras are optional equipment, and not included on all SLI6P systems). 1. Remove the dust cover plate (2) from the Trinocular Head Camera Port (1), by loosening the hex screw on the right side. -
Page 21: Specification
5. SPECIFICATION SLI6P INVERTED MICROSCOPE SPECIFICATIONS OPTICAL SYSTEM Infinity corrected HEAD Trinocular - 45° inclined, rotatable; 360 degrees, Trinocular head, interpupillary distance 50mm-75mm, light splitting ratio 100:100. OBJECTIVE Infinity plan positive phase contrast objectives (4x, 10x, 20x 40x, ), optional Infiniity, Plan Fluorite objectives in Brightfield and positive phase contrast EYEPIECE 2each, High eye point wide field plan eyepieces... - Page 22 FLUORESCENCE Excitation: ILLUMINATION AND Variable intensity 10W LED: 410 nm for DAPI excitation FLUORESCENCE FILTERS Variable Intensity 10W LED: 480-485nm for TRITC/mRFP excitation Emcitation: • 455nm +/-25nm for DAPI • 525nm +/- 18nm for FITC/eGFP • 605nm +/- 17.5nm for TRITC/mRFP Dichronic Mirrors Triple bandpass for DAPI, FITC, TRITC TRANSMITTED...
-
Page 23: Troubleshooting
5. TROUBLESHOOTING As the performance of microscope can’t play fully due to unfamiliar operations, the table below can provide some solutions. OPTICAL SYSTEM PROBLEM CAUSE SOLUTION REFERENCE PAGE The LED light is bright, but - The LED is burned out - Replace it with new one it’s dark in the field of view - The light adjusting... - Page 24 OPTICAL SYSTEM PROBLEM CAUSE SOLUTION REFERENCE PAGE Some parts of the image - Objectives is not place - Loosen it a little is not in the focal plane in the light path - The sample is place on the state incorrectly - The vessel has a curbed bottom The eyes feel tired easily.
-
Page 25: Electrical System
ELECTRICIAL SYSTEM PROBLEM CAUSE SOLUTION REFERENCE PAGE The LED does not work - No power supply - Check the connection of power cord - The LED is burned out - Replace it The LED burns out - The wrong LED is used - Replace it with the correct one quickly The field of view is now... -
Page 26: Led Flourescence Assembly
LED FLUORESCENCE ASSEMBLY PROBLEM CAUSE SOLOUTION REFERENCE PAGE The Fluorescence LEDs - The Fluorescence LED - Turn on the Fluorence LEDs with the have low intensity Module is not turned individual selection buttons on the from membrace panel, and ajdust intensity slider - The Multi-Illustration contrast button needs... -
Page 27: Customer And Techical Service
To return goods for repair or replacement, please 3. Wedge a block of foam between the fixed stage contact LAXCO Inc. Customer Service by one of the and the nosepiece / objectives. numbers above. Please be prepared to supply the following information: 4. - Page 28 9. REFERENCE Key Concepts MAGNIFICATION Magnification increases the apparent size of an object you are viewing through the microscope. Magnification by itself does not provide more information about an object unless there is also adequate resolution and contrast. The objective lens and oculars (eyepieces) determine magnification. Magnification = eyepiece value X objective lens value RESOLUTION Resolution is the ability to distinguish small objects that are close together.
- Page 29 Glossary of Microscopy Terms Abbe Condenser: A simple condenser comprised of C-mount: A type of camera adapter typically used two lenses; corrects for chromatic aberration. to connect video cameras to a microscope. Aberration: Term used to describe any CCD: Type of video camera using electronic chips inaccuracy in focusing of light;...
- Page 30 Glossary of Microscopy Terms Field Curvature: One of the optical distortions. Depth of Field: T he vertical distance in the sample When the center of the image is in focus and the through which features are simultaneously in focus. edgesthe field is said to “have curvature.” When High numerical aperture objectives have a “shallow the image is in focus from the center to the depth of field”...
- Page 31 Glossary of Microscopy Terms The light passing through a well-behaved phase-altering specimen (such as a cell) slows High Eyepoint: A design characteristic of eyepieces down by a quarter wave on that interaction, in which the back focal plane of the eyepiece is then another quarter-wave as it passes through raised about 18 mm above the top of the eyepiece the thickest part of the phase plate.
- Page 32 Glossary of Microscopy Terms Infinity Corrected Optics: A special optical design involving at least two lenses in which the object is Light, Polarized: Light in which the waves vibrate placed at the focal plane of the first lens, causing in only one direction, perpendicular to the the imaging rays to emerge parallel to the optic direction of travel.
- Page 33 Glossary of Microscopy Terms Microscope, Stereo: Phase Contrast: A standard microscope A contrast-enhancement configuration based on two independent imaging technique that detects phase objects. It uses a paths, separated by approximately 10-12 degrees, special ring, placed in the condenser to control resulting in a stereoscopic image characterized by location of the undiffracted light, and a matching great three-dimensionality and great depth of field.
- Page 34 Glossary of Microscopy Terms Primary Image: T Trinocular Port: he first magnified image formed A special eyepiece, usually in the microscope. narrower in design than conventional eyepieces, used in the photo tube of the microscope to Primary Image Plane: project a real image to the film plane or detector The location of the first of a camera system.
- Page 35 For service support: 425-686-3081, option 2 www.SeBaMicro.com For technical support: 425-686-3081, option 3 www.laxcoinc.com 1700074(1) - User Manual SLi6 Pro Inverted Fluorescent Microscope © 2021 LAXCO Inc. All rights reserved. Laxco™ is a registered trademark of Laxco Inc., in the U.S. and/or other countries.
Need help?
Do you have a question about the SLi6Pro and is the answer not in the manual?
Questions and answers