CONTENTS CONTENTS 1. Before Use - Operation Notice 1. Before Use - Operation Notice 2. Assembly 2. Assembly Assembly Scheme Assembly Steps 3. Operation 3. Operation Set Illumination Place Specimen Slide Focusing the Image Adjust Focusing Tension Setting Aperture Diaphragm Use the Oil Objective (100X) Binocular Head/Trinocular Head Mechancial Platforms...
BEFORE USE BEFORE USE OPERATION NOTICE OPERATION NOTICE 1. 1. As the microscope is a high precision instrument, always operate it with care, and avoid physical vibrations. 2. 2. Do not expose the microscope in the sun directly, either not in the high temperature, damp, dust or acute shake.
ASSEMBLING SCHEME ASSEMBLING SCHEME Following is the Assembling Scheme, and the numbers denote the assembling order. • • Before assembling, make sure there is no dust, dirt or other materials which will disturb it. Assemble carefully and do not scrap any part or touch the glass surface.
3. OPERATIONS 3. OPERATIONS 3-1 Set Illumination 3-1 Set Illumination 1. 1. Put through the power and adjust the light adjustment knob 1 until the illumination is comfortable for observation. Rotate the light adjustment knob in clockwise to raise the voltage and brightness.
3-5 Aperture Diaphragm 3-5 Aperture Diaphragm 1. 1. The aperture diaphragm decides the numerical aperture of the illumination system. Only when the N.A. of illumination system is matching with the N.A. of the objective, it can obtain better resolution and contrast, and also increase the depth of field.
3-7 Use binocular head /trinocular head 3-7 Use binocular head /trinocular head (Optional) (Optional) 3-7-1 Adjust the Interpupillary Distance 3-7-1 Adjust the Interpupillary Distance When observe with two eyes, hold the base of the prism and rotate them around the axis until there is only one field of view.
3-8-2 Aperture Diaphragm 3-8-2 Aperture Diaphragm 1. 1. The aperture diaphragm decides the numerical aperture of the illumination system. Only when the N.A. of illumination system is matching with the N.A. of the objective, it can obtain better resolution and contrast, and also increase the depth of field.
4. TROUBLESHOOTING 4. TROUBLESHOOTING As the performance of microscope can’t play fully due to unfamiliar operations, the table below can provide some solutions. PROBLEM PROBLEM CAUSE CAUSE SOLUTION SOLUTION 1. Optical Part 1. Optical Part (1) The LED light is bright, Field diaphragm is not large Enlarge the field diphragm.
PROBLEM PROBLEM CAUSE CAUSE SOLUTION SOLUTION 1. Optical Part 1. Optical Part (6) The eyes feel tired Interpupillary distance is wrong. Adjust the interpupillary distance. easily. The right field of view doesn’t superpose Eyepieces for the left eye and right eye with the left.
5. REFERENCE 5. REFERENCE Glossary of Microscopy Terms Glossary of Microscopy Terms Abbe Condenser: Abbe Condenser: A simple condenser comprised of two lenses; corrects for chromatic aberration. Aberration: Aberration: Term used to describe any inaccuracy in focusing of light; derived from physical limitations of lenses and optics.
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Glossary of Microscopy Terms Glossary of Microscopy Terms Eyepiece: Eyepiece: The optical component that provides the second step in magnification. Eyepoint: Eyepoint: The back focal plane of the eyepiece; the location at which the cornea of the eye is placed so that the information from the microscope can be imaged on the retina of your eye.
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Glossary of Microscopy Terms Glossary of Microscopy Terms High Eyepoint: High Eyepoint: A design characteristic of eyepieces in which the back focal plane of the eyepiece is raised about 18 mm above the top of the eyepiece to accommodate microscopists who wear glasses. Illumination, Axial: Illumination, Axial: A contrast-enhancement technique for improving edge contrast.
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Glossary of Microscopy Terms Glossary of Microscopy Terms Koehler Illumination: Koehler Illumination: An approach to microscope alignment that separates the illuminating set planes from an imaging set. A major goal of this approach is to illuminate evenly the back focal plane of the objective for maximum resolution and evenly illuminated background.
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Glossary of Microscopy Terms Glossary of Microscopy Terms Phase: Phase: A property of light in which waves are “in step” with each other. See also “In phase.” Phase Contrast: Phase Contrast: A contrast-enhancement technique that detects phase objects. It uses a special ring, placed in the condenser to control location of the undiffracted light, and a matching phase plate, placed in the back focal plane of the objective.
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Glossary of Microscopy Terms Glossary of Microscopy Terms Telan Lens: Telan Lens: An auxiliary lens used in conjunction with an infinity corrected objective to bring light to a proper focus. In some systems, the telan lens will also correct the objective’s residual chromatic aberration. See also “Tube lens.”...
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