CONTENTS CONTENTS 1. Operation Notice 2. Instrument Components 3. Assembly Objective Assembly Stage Plate Assembly Eyepiece Assembly Phase-contrast Slider Assembly Color Filter Assembly (For Transmitted Illumination) Stage Extension and Mechanica Replacing the LED 4. Operation Power Cord Set Illumination Focus Adjustment Focus Tension Adjustment Specimen Placement Mechanical Stage (Optional)
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1. Operation Notice 1. Operation Notice 1. The microscope is a precision instrument, please operate carefully: avoid vibrating or jolting the microscope during operation. 2. Do not operate the microscope under conditions of direct sunlight, high temperature, high humidity, dust, or close to sources of vibration. Ensure the work surface is flat.
3. 3. Assembly Assembly Objective Assembly (Fig 6,7) Objective Assembly (Fig 6,7) 1. Rotate the coarse focus knob (1), until the objective nosepiece is at the lowest position. (Fig 6) 2. Install the objectives into the microscope nosepiece from the lowest magnification to the highest in a clockwise direction from the back of the microscope.
3. 3. Assembly Assembly Eyepiece Assembly (Fig 9) Eyepiece Assembly (Fig 9) 1. Remove the eyetube cover (1). 2. Insert the eyepiece (2) into the eyetube. Fig. 9 Phase-contrast Slider Assembly (Fig 10) Phase-contrast Slider Assembly (Fig 10) 1. Place phase-contrast slider (1) letter side up, into the holder from the right to the left.
3. Assembly 3. Assembly Stage Extension and Mechanical Stage Stage Extension and Mechanical Stage Assembly Optional (Fig 12) Assembly Optional (Fig 12) The stage extension can be attached on both the left and right side of the stage to increase the stage size. The stage extension and the attachable mechanical stage both cannot be attached on the same side of the stage.
4. Operation 4. Operation Power Cord (Fig 14) Power Cord (Fig 14) 1. Turn the power switch to the “O” OFF position before connecting the power cord. 2. Insert the plug into the outlet on the back of the microscope. 3.
4. Operation 4. Operation Specimen Placement (Fig 17) Specimen Placement (Fig 17) Several vessel holders are available for use with or without the mechanical stage. Refer to the Parts and Accessories section of this guide, for ordering information. 1. Place vessel holder on stage (2) or in mechanical stage clip.
4. Operation Diopter Adjustment (Fig 19) Diopter Adjustment (Fig 19) 1. Look through the right eyepiece with your right eye and focus on a specimen using the coarse and fine focus adjustment knobs. 2. Once the image is clear in the right eyepiece, look through the left eyepiece with your left eye, turn the diopter adjustment ring (1) until the image is clear.
4. Operation 4. Operation Color Filters (Fig 22) Color Filters (Fig 22) Color filters are used to enhance the viewing of a specimen and in photomicrography. Color filters (2) can overlay in the filter holder (1); ensure that they lay flat in the filter holder and the thickness doesn’t exceed 11mm.
4. Operation 4. Operation Aperture Diaphragm Adjustment (Fig 24) Aperture Diaphragm Adjustment (Fig 24) 1. The aperture diaphragm determines the numerical aperture of the illumination in brightfield observation. If the N.A. of illumination matches the N.A. of the objective, you can obtain better resolution and contrast, and increase the depth of field.
4. Operation 4. Operation Centering the Phase Contrast Annuli Centering the Phase Contrast Annuli (Fig 27 & 28) (Fig 27 & 28) 1. Place specimen on the stage and focus it. 2. Make sure the matched phase ring (in the phase-contrast objective) and the light ring (in the phase-contrast slider) are in the light path.
4. Operation 4. Operation C-mount Assembly (Fig 29) C-mount Assembly (Fig 29) 1. Loosen the lock-screw (1) on the trinocular head and remove the dust-cover (2). 2. Remove the dust-cover caps of the c-mount adapter (3) and the camera. Insert the screw thread end of c-mount adapter onto the camera, and then install the c-mount into the camera port.
6. Troubleshooting 6. Troubleshooting SYMPTOMS POTENTIAL CAUSE SOLUTION LED is bright but the light in the The LED has burned out Replace LED field of view is not bright enough Dimmer is turned down Turn up the dimmer Side of the field of view is dark or The nosepiece is not in the correct Reposition nosepiece not even...
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6. Troubleshooting 6. Troubleshooting SYMPTOMS POTENTIAL CAUSE SOLUTION Eyes tire easily seeing two fields Interpupillary distance is wrong Adjust the interpupillary distance of view Diopter adjustment is wrong Adjust the diopter Eye not accustomed to binocular See the entire view-field with observation steady observation MECHANICS...
3. Returned Goods Policy for Repair or Replacement Parts 4. To return goods for repair or replacement, please contact Laxco Customer Service at 425-686-3081. Please be prepared to supply the following information: •...
8. REFERENCE 8. REFERENCE Glossary of Microscopy Terms Glossary of Microscopy Terms Abbe Condenser: Abbe Condenser: A simple condenser comprised of two lenses; corrects for chromatic aberration. Aberration: Aberration: Term used to describe any inaccuracy in focusing of light; derived from physical limitations of lenses and optics.
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Glossary of Microscopy Terms Glossary of Microscopy Terms Eyepiece: Eyepiece: The optical component that provides the second step in magnification. Eyepoint: Eyepoint: The back focal plane of the eyepiece; the location at which the cornea of the eye is placed so that the information from the microscope can be imaged on the retina of your eye.
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Glossary of Microscopy Terms Glossary of Microscopy Terms High Eyepoint: High Eyepoint: A design characteristic of eyepieces in which the back focal plane of the eyepiece is raised about 18 mm above the top of the eyepiece to accommodate microscopists who wear glasses. Illumination, Axial: Illumination, Axial: A contrast-enhancement technique for improving edge contrast.
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Glossary of Microscopy Terms Glossary of Microscopy Terms Koehler Illumination: Koehler Illumination: An approach to microscope alignment that separates the illuminating set planes from an imaging set. A major goal of this approach is to illuminate evenly the back focal plane of the objective for maximum resolution and evenly illuminated background.
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Glossary of Microscopy Terms Glossary of Microscopy Terms Phase: Phase: A property of light in which waves are “in step” with each other. See also “In phase.” Phase Contrast: Phase Contrast: A contrast-enhancement technique that detects phase objects. It uses a special ring, placed in the condenser to control location of the undiffracted light, and a matching phase plate, placed in the back focal plane of the objective.
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Glossary of Microscopy Terms Glossary of Microscopy Terms Telan Lens: Telan Lens: An auxiliary lens used in conjunction with an infinity corrected objective to bring light to a proper focus. In some systems, the telan lens will also correct the objective’s residual chromatic aberration.
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