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Laxco SLi3Pro User Manual

Inverted fluorescence microscope
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SLi3Pro Inverted
Fluorescence Microscope
Version 2.0
User's Manual

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Summary of Contents for Laxco SLi3Pro

  • Page 1 SLi3Pro Inverted Fluorescence Microscope Version 2.0 User’s Manual...

  • Page 2: Table Of Contents

    CONTENTS CONTENTS 1. I ntroduction 4.2.13 Darkroom Light Shield 4.2.14 Installing Fluorescence illum. 18 Disclaimer 4.2.15 Using Floorescence LEDs Safety Conventions Prior to use 5 Camera Installation Installing SeBaCam 2. Components 6. Specification 21, 22 3. Assembly 3.1 Assembly Precautions 7.

  • Page 3: Introduction

    DISCLAIMER DISCLAIMER Information provided by Laxco Inc. is believed to be accurate and reliable. However, no responsibility is assumed by Laxco Inc. for its use. The information contained in this publication is subject to change without notice. Words that Laxco Inc. considers trade- marks have been identified as such.

  • Page 4: Prior To Use

    PRIOR TO USE PRIOR TO USE 1. The microscope is a precision instrument. Please operate properly avoiding vibration or sudden jolting the microscope during operation. 2. Do not operate the microscope in direct sunlight, high temperature, high humidity, dusty environment, or close to sources of vibration.

  • Page 5: Maintenance

    MAINTENANCE MAINTENANCE 1. Utilizing a lens tissue moistened with a small amount of lens cleaning solution, gently wipe the objective lens removing all oil and fingerprints on the objective surfaces. Lens cleaning solution may be flammable. Turning on or off electronic devices (including the microscope) may produce a spark which could ignite the lens cleaning solution.

  • Page 6: Components

    2. COMPONENTS 2. COMPONENTS LED Lamphouse LED Lamphouse Aperture Diaphragm Adjusting Slider Aperture Diaphragm Adjusting Slider Eyepieces Eyepieces Camera Port Camera Port Darkroom Slider Darkroom Slider Fixed Stage Fixed Stage Trinocular Body Trinocular Body Nosepiece Nosepiece Coarse & Fine Focus Knobs Coarse &...

  • Page 7: Assembly

    3. ASSEMBLY 3. ASSEMBLY 3.1 ASSEMBLY PRECAUTIONS 3.1 ASSEMBLY PRECAUTIONS Following are the recommended assembly steps with each step numbered denoting the assembling order. Before assembling, make sure there is no dust, dirt or other materials which will effect the operation. Assemble carefully while avoiding contact with any glass surfaces.

  • Page 8: Objectiveassembly

    3-2-1 OBJECTIVE ASSEMBLY 3-2-1 OBJECTIVE ASSEMBLY Rotate the coarse focus knob, until the objective nosepiece is at the lowest position. 2. Install the objectives into the microscope nosepiece from the lowest magnification to the highest in a clockwise direction from the back of the microscope.

  • Page 9: Eyepiece Assembly

    3-2-3 EYEPIECE ASSEMBLY 3-2-3 EYEPIECE ASSEMBLY Eyepiece Eyepiece Remove the eyetube cover. 2. Insert the eyepiece into the eyetube. 3-2-4 PHASE - CONTRAST SLIDER ASSEMBLY 3-2-4 PHASE - CONTRAST SLIDER ASSEMBLY 1. Place phase-contrast slider letter side up, into the holder from the right to the left.

  • Page 10: Color Filter Assembly

    3-2-5 COLOR FILTER ASSEMBLY 3-2-5 COLOR FILTER ASSEMBLY (FOR TRANSMITTED ILLUMINATION) (FOR TRANSMITTED ILLUMINATION) 1. Turn the microscope off and allow the filter to cool before changing. 2. Slide out the filter holder and place the color filter in to the filter holder. 3.

  • Page 11: Power Cord

    Attach the stage extension or mechanical Attach the stage extension or mechanical stage as follows: stage as follows: 1. Screw the lock-screws (1) into the bottom of the stage extension or mechanical stage. 2. Screw it into the bottom of the stage. 3-2-7 POWER CORD 3-2-7 POWER CORD 1.

  • Page 12: Operation

    4. OPERATION 4. OPERATION 4-2 SPECIMEN PLACEMENT 4-2 SPECIMEN PLACEMENT Plug the microscope into a power outlet and turn the power switch to the ON position. 2. Press the BF/PH button located at the lower left on the membrane control panel on the front of the microscope body.

  • Page 13: Focus Adustment

    Operation of the attachable Operation of the attachable mechanical stage mechanical stage 1. Place the multi-well plate on the me- chanical stage holder when using 96 or 24 well plate. Stage Holder Stage Holder 2. Vessel Holders: Specimen Slide Specimen Slide •...

  • Page 14: Focus Tension Adjustment

    4-4 FOCUS TENSION ADJUSTMENT 4-4 FOCUS TENSION ADJUSTMENT If the coarse focus knob is difficult to rotate or the objective nosepiece “drifts” or loses focus this can typically be corrected by adjusting the focus tension. Rotate the tension adjustment ring according to the arrow direction in the figure to tighten the focus tension;...

  • Page 15: Interpupillary Distance Adjustment

    4-6 INTERPUPILLARY 4-6 INTERPUPILLARY DISTANCE ADJUSTMENT DISTANCE ADJUSTMENT While looking through both eyepieces, move the eyepieces together or apart until the field appears as one circle and viewing is comfortable. 2. The number on the index that lines up with the “.” On the side is the inter-pupillary distance of your eyes.

  • Page 16: Use Of Eyecup

    4-8 USE THE EYE-CAP 4-8 USE THE EYE-CAP 1. If the user wears glasses, turn the eyecup downward to prevent the Eye- Eye- glasses from touching the eyepiece and avoid damage to the glasses and the eyepiece. 2. Raise the eyecup for users without glasses.

  • Page 17: Condenser Lens Removal

    4-10 CONDENSER LENS REMOVAL 4-10 CONDENSER LENS REMOVAL The condenser lens can be removed to view specimens in large vessels, or cell stacks (up to 5 layers). Unscrew the condenser lens to increase the working Condenser Lens distance 4-11 Multi-Illumination 4-11 Multi-Illumination Contrast TM TM (MIC)

  • Page 18: Darkroom Light Shield

    4-12 DARKROOM LIGHT 4-12 DARKROOM LIGHT SHIELD SHIELD The Darkroom Light Shield may be utilized to block extraneous room light while us- ing the SLI3P in Fluorescence Mode. The shield, located behind the condenser may be lowered and rotated, close to the vessel, well plate, or slide to improve the Fluorescence signal to noise ratio.

  • Page 19: Using Floorescence Leds

    4-14 OPERATING THE 4-14 OPERATING THE FLURESCENCE LEDs FLURESCENCE LEDs ON SLI3P CONTROL ON SLI3P CONTROL PANEL PANEL Select the desired LED from the Membrane Touch Panel, and press the button. Once for ON, Once again for OFF. 2. Adjust the intensity using the slider on the right hand side of the panel.

  • Page 20: Camera Installation

    5. CAMERA 5. CAMERA 5.1 CAMERA INSTALLATION 5.1 CAMERA INSTALLATION Select the appropriate c-mount adapter. (Note: c-mounts and cameras are option- al equipment, and not included on ALL SLI3P systems). 1. Remove the dust cover plate from the Trinocular Head Camera Port, by loosening the hex screw on the right side.

  • Page 21: Specification

    6. SPECIFICATION 6. SPECIFICATION SLI3P INVERTED MICROSCOPE SPECIFICATIONS SLI3P INVERTED MICROSCOPE SPECIFICATIONS OPTICAL SYSTEM OPTICAL SYSTEM Infinity corrected HEAD HEAD Trinocular - 45° inclined, rotatable; 360 degrees, Trinocular head, interpupillary distance 50mm-75mm, light splitting ratio 100:100. OBJECTIVE OBJECTIVE Infinity plan positive phase contrast objectives (4x, 10x, 20x 40x, ), optional Infiniity, Plan Fluorite objectives in Brightfield and positive phase contrast EYEPIECE...
  • Page 22 FLUORESCENCE FLUORESCENCE Excitation: ILLUMINATION AND ILLUMINATION AND Variable intensity 10W LED: 410 nm for DAPI excitation FLUORESCENCE FILTERS FLUORESCENCE FILTERS Variable Intensity 10W LED: 480-485nm for TRITC/mRFP excitation Emcitation: • 455nm +/- 25nm for DAPI • 525nm +/- 18nm for FITC/eGFP •...

  • Page 23: Troubleshooting

    7. TROUBLESHOOTING 7. TROUBLESHOOTING As the performance of microscope can’t play fully due to unfamiliar operations, the table below can provide some solutions. PROBLEM PROBLEM CAUSE CAUSE SOLUTION SOLUTION REFERENCE REFERENCE PAGE PAGE The LED light is bright, - The LED is burned out - Replace it with new one but it’s dark in the field of view...

  • Page 24: Optical System

    OPTICAL SYSTEM OPTICAL SYSTEM PROBLEM PROBLEM CAUSE CAUSE SOLUTION SOLUTION REFERENCE PAGE REFERENCE PAGE Some parts of the im- - Objectives is not place - Loosen it a little age is not in the focal in the light path plane - The sample is place on the state incorrectly - The vessel has a curbed...

  • Page 25: Electrical System

    ELECTRICIAL SYSTEM ELECTRICIAL SYSTEM PROBLEM PROBLEM CAUSE CAUSE SOLUTION SOLUTION REFERENCE REFERENCE PAGE PAGE The LED does not - No power supply - Check the connection of work power cord - The LED is burned - Replace it The LED burns out - The wrong LED is - Replace it with the correct quickly...
  • Page 26 PROBLEM PROBLEM CAUSE CAUSE SOLUTION SOLUTION REFERENCE REFERENCE PAGE PAGE The Fluorescence LEDs - The fluorescence LED - Turn on the fluorescence have low intensity module is not turned LEDs with the individual selection buttons on the from membrane panel, - The Multi-Illustration and adjust intensity slider contrast button needs...

  • Page 27: Customer And Technical Service

    IMPORTANT! Improperly packaged will be insurable. Vertify your order requirements • • Distributor information, if applicable before packing and shipping A Laxco representative will issue you a Return Materials Authorization (RMA) number. Please label the outside of your shipping container with the RMA number.

  • Page 28: Reference

    9. REFERENCE 9. REFERENCE KEY CONCEPTS KEY CONCEPTS MAGNIFICATION MAGNIFICATION Magnification increases the apparent size of an object you are viewing through the mi- croscope. Magnification by itself does not provide more information about an object unless there is also adequate resolution and contrast. The objective lens and oculars (eye- pieces) determine magnification.Magnification = eyepiece value X objective lens value.
  • Page 29 Glossary of Microscopy Terms Glossary of Microscopy Terms Abbe Condenser: Abbe Condenser: C-mount: C-mount: A simple condens- A type of camera adapter typ- er comprised of two lenses; corrects for ically used to connect video cameras to a chromatic aberration. microscope.
  • Page 30 Glossary of Microscopy Terms Glossary of Microscopy Terms Field Curvature: Field Curvature: One of the optical Depth of Field: Depth of Field: The vertical distance in distortions. When the center of the image the sample through which features are is in focus and the edgesthe field is said simultaneously in focus.
  • Page 31 Glossary of Microscopy The light passing through a well-behaved phase-altering specimen (such as a cell) High Eyepoint: High Eyepoint: A design characteristic of slows down by a quarter wave on that eyepieces in which the back focal plane of interaction, then another quarter-wave as the eyepiece is raised about 18 mm above it passes through the thickest part of the the top of the eyepiece to accommodate...
  • Page 32 Glossary of Microscopy Terms Glossary of Microscopy Terms Infinity Corrected Optics: Infinity Corrected Optics: A special optical design involving at least two lenses Light, Polarized: Light, Polarized: Light in which the in which the object is placed at the focal waves vibrate in only one direction, per- plane of the first lens, causing the imaging pendicular to the direction of travel.
  • Page 33 Glossary of Microscopy Terms Glossary of Microscopy Terms Microscope, Stereo: Microscope, Stereo: Phase Contrast: Phase Contrast: A standard mi- A contrast-enhance- croscope configuration based on two in- ment technique that detects phase ob- dependent imaging paths, separated by jects. It uses a special ring, placed in the approximately 10-12 degrees, resulting in a condenser to control location of the undif- stereoscopic image characterized by great...
  • Page 34 Glossary of Microscopy Terms Glossary of Microscopy Terms Primary Image: Primary Image: The first magnified im- Trinocular Port: Trinocular Port: A special eyepiece, usu- age formed in the microscope. ally narrower in design than conventional eyepieces, used in the photo tube of the Primary Image Plane: Primary Image Plane: microscope to project a real image to the...
  • Page 35 For service support: 425-686-3081, option 2 Mill Creek, WA 98012 For technical support: 425-686-3081, option 3 www.SeBaMicro.com www.laxcoinc.com SLI3P-SLI-MKT-DOC-1700107(2) © 2023 LAXCO Inc. All rights reserved. Laxco™ is a registered trademark of Laxco Inc., in the U.S. and/or other countries.

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