Shimadzu Nexera X2 Instruction Manual
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Shimadzu Nexera X2 Instruction Manual

Shimadzu Nexera X2 Instruction Manual

Photodiode array uv-vis detector for ultra high performance liquid chromatograph
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228-91112C
Apr. 2015
PHOTODIODE ARRAY UV-VIS DETECTOR
FOR SHIMADZU ULTRA HIGH PERFORMANCE LIQUID CHROMATOGRAPH
Nexera X2 SPD-M30A
Instruction Manual
Read this manual thoroughly before you use the product.
Keep this manual for future reference.

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  • Page 1 228-91112C Apr. 2015 PHOTODIODE ARRAY UV-VIS DETECTOR FOR SHIMADZU ULTRA HIGH PERFORMANCE LIQUID CHROMATOGRAPH Nexera X2 SPD-M30A Instruction Manual Read this manual thoroughly before you use the product. Keep this manual for future reference.
  • Page 2 This page is intentionally left blank.
  • Page 3 1 Nexera X2 SPD-M30A Instruction Manual (S228-91112) 2 Nexera X2 Safety Guideline (S228-91060) Before using the product, be sure to read the separate manual "Nexera X2 Safety Guideline", which describes product warranty, after-sales service, and precaution items for safety use. For installation procedure, hardware validation, etc. for the Nexera X2 series involving this product, refer to the "Nexera X2 System Guide", provided with the...
  • Page 4 â companies. The TM and symbols are omitted in this manual. • Nexera is a registered trademark of Shimadzu Corporation in the United States and Europe (Office for Harmonization in the Internal Market). Nexera X2 SPD-M30A...
  • Page 5 The following symbols are used in this manual: Indication Meaning Indicates an action that must not be performed. Indicates an action that must be performed. Indicates information provided to improve product performance. Indicates the location of related reference information. Nexera X2 SPD-M30A...

  • Page 6: Table Of Contents

    Configuring Network Settings (Web Browser) ......3.3.3 Configuring User IDs and Passwords ......... 3.3.4 Exiting System Configuration Basic Operation Setting Parameters ..........Analog Output Usage and Settings ............4.2.1 Analog Output Overview ....4.2.2 Configuring Analog Output Using LabSolutions Nexera X2 SPD-M30A...
  • Page 7 Prior to Inspection and Maintenance ....7.1.2 Periodic Inspection and Maintenance List ....7.1.3 Check After Inspection and Maintenance Cleaning the Flow Cell ................7.2.1 Removing the Flow Cell ............7.2.2 Cleaning ........7.2.3 Installing the Flow Cell Nexera X2 SPD-M30A...
  • Page 8 External Input/Output Terminals Software Installation ........Specifications ..................8.3.1 Performance Specifications .......... 8.3.2 Standard Flow Cell ..........8.3.3 Detector Unit Replacement Parts ..................8.4.1 Consumable Parts ..........8.4.2 Necessary Parts Technical Information Regarding Networks ..Index ............Nexera X2 SPD-M30A...

  • Page 9: Overview And Configuration

    Overview and Configuration Overview The SPD-M30A is a photodiode array, UV-Vis detector designed for use with a Shimadzu ultra high performance liquid chromatograph system (HPLC), and was developed to satisfy demands for greater accuracy and sensitivity in analysis. Other components that comprise a complete HPLC system, including the solvent delivery module, autosampler, column oven, system controller, and LC workstation software are to be purchased separately.
  • Page 10 If using a LAN environment, a remote PC can be used to configure instrument settings and monitor instrument operation. Connecting to this instrument using a Utilty software (SPD-M30A Utility) allows remote setting of analysis conditions and starting of analysis. Nexera X2 SPD-M30A...

  • Page 11: Component Parts

    Power cord (for 230 V) S071-60825-51 -48, -58 only Power cord (for 120 V) S071-60821-08 -42 only SPD-M30A Instruction S228-91112 manual (English version) Nexera X2 Safety S228-91060 Guideline (English version) Syringe needle S228-57027 Syringe, 1ML S228-57028 Coupling 1.6-0.8C S228-40998-10 Male nuts 0.8MN-PEEK...

  • Page 12: Items Required For Operation

    Must satisfy the recommended PC PC system specifications in following table *1 For details on hubs and Ethernet cables that can be used, contact your Shimadzu representative. n Recommended PC Specifications A PC and optional equipment with the following specifications are required.

  • Page 13: Optional Parts

    1.5 Optional Parts Optional Parts Optional units available for this instrument are listed below. For information about other optional units not listed below, contact your Shimadzu representative. n Option オプション名 部品番号 備考 Low-pressure Hg S207-21594-41 (mercury) lamp set For inspection of the wavelength accuracy...

  • Page 14: Part Names And Functions

    "7.9 Replacing the Dust Filter" P.95 Open this cover to install or perform maintenance on the Front cover flow cell. Power switch Switches power ON/OFF. Left-side cover Open this cover to replace the lamp. Nexera X2 SPD-M30A...

  • Page 15: Behind The Front Cover And Left-Side Cover

    Coupling holder The retaining plate and screw secure the coupling in place. Installation is performed using the coupling. • "2.3.5 Plumbing for High Pressure Flow Line (Gradient System)" in the Nexera X2 System Guide for the tubing procedure. Flow cell •...

  • Page 16: Base Panel

    "2.3.1 Connecting Leakage Drain Tubing" in the Nexera X2 System Guide Used to protect the internal measurement components from vibrations during transport. It is removed after installation. Shipping screw "2.1.8 Installation of SPD-M30A" in the Nexera X2 System Guide Nexera X2 SPD-M30A...

  • Page 17: Back

    Fuse holders Fuses are set inside. Used for connection of the power cable. Power cord connector "2.2.1 Power Supply Connection" in the Nexera X2 System Guide Fan for internal cooling Cooling fan (Keep a minimum of 100 mm between the rear of the instrument and the wall.)

  • Page 18: Names And Functions Of Displays

    The LED blinks while the lamp is warming up. Illuminates when the instrument is connected to the system CONNECT controller and PC. Illuminates green when the power is ON. Status indicator Illuminates red when an error occurs. Illuminates orange during sleep mode. Nexera X2 SPD-M30A...

  • Page 19: Precautions On Operation

    • Lamp light intensity check • Wavelength accuracy check "7 Maintenance" P.73 For items that should be checked periodically to ensure performance, refer to "3.10 Validation of the SPD-M30A UV-VIS Photodiode Array Detector" in the Nexera X2 System Guide. Nexera X2 SPD-M30A...

  • Page 20: Preparation

    In addition, if a buffer solution is used as the mobile phase, purge the buffer solution with water after the completion of analysis. Crystals that form from the buffer solution drying can clog the flow cell lines. Nexera X2 SPD-M30A...

  • Page 21: Turning The Power On/Off

    • Whether the D2 lamp is turned on or not is determined by the setting at the time of the last instrument shutdown. • If an error occurs, the status indicator LED will illuminate red. Nexera X2 SPD-M30A...

  • Page 22: Configuring The System

    The user ID and password above are for accessing the SPD-M30A from a web browser and for launching SPD-M30A Utility from a PC without LabSolutions installed. To define user ID and password for launching SPD-M30A Utility from a PC with LabSolutions installed, perform the setting on LabSolutions. "5.3.1 Launching SPD-M30A Utility" P.38 Nexera X2 SPD-M30A...

  • Page 23: Configuring Network Settings (Web Browser)

    Turn ON the PC used for controlling the instrument. Launch the web browser on the PC. Enter the IP address of the instrument in the [Address] field of the browser. (http://<IP address>/) Default IP address: 192.168.200.98 "5.2 Initializing Network Settings, User IDs, and Passwords" P.37 Nexera X2 SPD-M30A...
  • Page 24 Version display Displays the firmware version. Enter the user ID for connection. [User ID] "3.3.1 User Rights" P.14 Enter the corresponding password. [Password] "3.3.1 User Rights" P.14 [Login] button Executes user login. The administrator screen is displayed. Nexera X2 SPD-M30A...
  • Page 25 [Logout] button Log out of the administrator screen. Configure network information. (This tab is selected when [Configuration] tab a user logs in.) [User Management] tab Configure user information. [Log] tab Display log information stored on the instrument. Nexera X2 SPD-M30A...
  • Page 26 IP address into the web browser and log in again. Name Function Set the IP address. [IP Address] (Default: 192.168.200.98) Set the subnet mask. [Subnet Mask] (Default: 255.255.255.0) Set the default router. [Default Gateway] (Default: 0.0.0.0) Set the Ethernet communication speed. [Ethernet Mode] (Default: "Auto Detect") Nexera X2 SPD-M30A...
  • Page 27 Mode Name Description Auto Detect Automatic speed setting (default) 10Mbps/Half Duplex Half duplex at 10 Mbps 10Mbps/Full Duplex Full duplex at 10 Mbps 100Mbps/Half Duplex Half duplex at 100 Mbps 100Mbps/Full Duplex Full duplex at 100 Mbps Nexera X2 SPD-M30A...

  • Page 28: Configuring User Ids And Passwords

    To define user ID and password for launching SPD-M30A Utility from a PC with LabSolutions installed, perform the setting on LabSolutions. • "3.3.1 User Rights" P.14 • "Launching SPD-M30A Utility (PC with LabSolutions Installed)" P.38 • "Launching SPD-M30A Utility (PC without LabSolutions Installed)" P.41 Nexera X2 SPD-M30A...
  • Page 29 Set the user information for operating the instrument using SPD-M30A Utility. Name Function [Operator ID] Set the operator ID. (Default: Operator) [New Password] Set the password. (Default: None) Enter the same password as [New Password] for [Password (Confirmation)] confirmation. Nexera X2 SPD-M30A...

  • Page 30: Exiting System Configuration

    3 Preparation 3.3.4 Exiting System Configuration Click [Logout] at the top of the screen to log out. Exit the web browser. Nexera X2 SPD-M30A...

  • Page 31: Basic Operation

    5, 10, 25, 80, Noise decreases. 160, 240, Time constant * If overly increased, 320, 640, Switch 640 ms Sensitivity (response) sharp peaks flatten 1280, 2000 out. ms, 0 (OFF) Lamp ON/OFF D2, OFF Switch Polarity +, - Switch Nexera X2 SPD-M30A...
  • Page 32 This instrument is equipped with 4 channels of analog output terminals, which enables it to be used as a multi channel UV detector. For details on setting the wavelength, see "4.2 Analog Output Usage and Settings" P.27. Nexera X2 SPD-M30A...
  • Page 33 This is the cycle at which data is output. The output data is the average of data within 1 sampling cycle. Example 1: Exposure time: 20 ms, sampling time: 2000 ms  Data collected every 20 ms is recorded every 2000 ms after signal processing is applied. Nexera X2 SPD-M30A...
  • Page 34 A data volume limit of 128 MB, measurement wavelength range of 200 nm to 350 nm, sampling time of 10 ms, and spectral resolution of 1024 will allow data collection for a maximum of approx. 18 minutes. Nexera X2 SPD-M30A...

  • Page 35: Analog Output Usage And Settings

    "5.3.6 Setting Analysis Parameters" P.54 "5.3.7 Executing Analog Output of Absorbance" P.57 Analog Output Specifications Anlog output 0.5, 1, 1.25, 2, 2.5, 4 AU/V (selectable and configurable) range: Polarity: +, - (selectable and configurable) Voltage output -0.1 to +1.0 (V) range: Nexera X2 SPD-M30A...
  • Page 36 SOR.Lv (Limit Detectable Level Value)" in the FRC-10 instruction manual. • If the sampling time is 25 ms or lower, absorbance change may not be output accurately through the analog output. In this case, use the digital signal output on the [ETHERNET] connector. Nexera X2 SPD-M30A...

  • Page 37: Configuring Analog Output Using Labsolutions

    4.2 Analog Output Usage and Settings 4.2.2 Configuring Analog Output Using LabSolutions Launch LabSolutions. For details on LabSolutions operation, refer to the LabSolutions instruction manual. Start the [Instrument Parameters View] and select [Advanced]. Nexera X2 SPD-M30A...
  • Page 38 The analog output signal is the average of absorbance data  the [Bandwidth] configured bandwidth around the set wavelength. [Output Range] Set the range of the analog signal output voltage. Set the polarity (positive/reversed voltage) that increases the [Polarity] absorbance level. Nexera X2 SPD-M30A...

  • Page 39: Application Operation

    Displaying various instrument logs Displaying system check reports Displaying the condition of consumable parts 5.1.1 Displaying the Error Log Launch the web browser and log onto the instrument. "Launching the Web Browser and Connecting to the Instrument" P.15 Nexera X2 SPD-M30A...
  • Page 40 Description Remarks Displays the number of the entry in the error [No.] log. Displays the date and time that each error Date and time display/power [Date] occurred. ON time display [Code] / Displays the error message. [Message] Nexera X2 SPD-M30A...

  • Page 41: Displaying The Operation Log

    5.1.2 Displaying the Operation Log Launch the web browser and log onto the instrument. "Launching the Web Browser and Connecting to the Instrument" P.15 Click the [Log] tab after logging in. The options on the [Log] tab are displayed. Nexera X2 SPD-M30A...

  • Page 42: Displaying The Maintenance Log

    5.1.3 Displaying the Maintenance Log Launch the web browser and log onto the instrument. "Launching the Web Browser and Connecting to the Instrument" P.15 Click the [Log] tab after logging in. The options on the [Log] tab are displayed. Nexera X2 SPD-M30A...

  • Page 43: Displaying The System Check Report

    LC workstation LabSolutions. Launch the web browser and log onto the instrument. "Launching the Web Browser and Connecting to the Instrument" P.15 Click the [Log] tab after logging in. The options on the [Log] tab are displayed. Nexera X2 SPD-M30A...
  • Page 44 Displays settings, such as the leak sensor threshold value and the D2 lamp Values (1) illumination time, and values that represent the state of the instrument. Displays the system check judgment Values (2) criterion or values related to Values (1). Nexera X2 SPD-M30A...

  • Page 45: Displaying The Condition Of Consumable Parts

    [System] been supplied to the instrument Initializing Network Settings, User IDs, and Passwords If the instrument's network settings, administrator ID or password, operator ID or password are forgotten, initialize the instrument's parameters according to the following procedure. Nexera X2 SPD-M30A...

  • Page 46: Operation Using Spd-M30A Utility

    • Enabling/disabling M20A compatibility mode 5.3.1 Launching SPD-M30A Utility This section explains how to use SPD-M30A Utility. n Launching SPD-M30A Utility (PC with LabSolutions Installed) Exit LabSolutions analysis. If the LabSolutions analysis program is running, exit the analysis program. Nexera X2 SPD-M30A...
  • Page 47 Select the desired options in the [Tool Launcher] window. Select [SPD-M30A Utility] for [Application]. Select the analysis instrument that is connected to SPD-M30A for maintenance for [Detector Name]. Click [Run]. Select a username in the [User ID], enter the password, and click [OK]. Nexera X2 SPD-M30A...
  • Page 48 Users without the "instrument adjustment" privilege cannot launch SPD-M30A Utility. LabSolutions User Right Instrument User Right Modification of (Operation Rights for SPD-M30A System Check Instrument System Check Utility) Execution Adjustment Settings  Operator rights    Administrator rights Nexera X2 SPD-M30A...
  • Page 49 By double clicking on the SPD-M30A Utility shortcut located in the provided CD, the SPD- M30A Utility application "SPDM30AUtility.exe" is launched. After the application is launched, the [SPD-M30A User Authentication] window is displayed for selecting the instrument to connect. Nexera X2 SPD-M30A...
  • Page 50 Only SPD-M30A instruments are displayed in the instrument list. M10Avp and M20A instruments are not displayed. Note that SPD-M30A instruments operating in M20A compatibility mode are displayed in the instrument list and "SPD-M20A" appears in the server type information field. Nexera X2 SPD-M30A...
  • Page 51 Check the user ID and password. Try connecting again or changing Failed to authenticate the instrument for connection by User ID authentication failed for SPD-M30A. selecting [Select Instrument to Connect] on the [Instrument] menu in the launcher window. Nexera X2 SPD-M30A...
  • Page 52 SPD-M30A Utility [Function] - help file. Normally, however, [Absorbance Linearity [F4] check absorbance linearity by Check] the procedure described in the Nexera X2 System Guide "3.10 Validation: Photodiode array detector". [Function] - Perform a temperature check of [F5] P.58 [Temperature Check] the lamp compartment.

  • Page 53: Measuring Light Intensity

    Measure and display the light energy received by the PDA elements. n Procedure for Light Intensity Measurement Flush the flow cell with water. Confirm that there is no leakage. Launch SPD-M30A Utility. "5.3.1 Launching SPD-M30A Utility" P.38 Nexera X2 SPD-M30A...
  • Page 54 Measurement is performed with the elements shielded from light (with the optical path shutter closed). The measurement result is saved as the dark current value. Dark current measurement is only performed once after connecting to the instrument. It does not need to be performed before every measurement. Nexera X2 SPD-M30A...
  • Page 55 Displays a wavelength scale or element scale depending on the X axis X axis scale mode. Displays the light intensity value scale. An automatic scale or Y axis fixed scale is displayed depending on the Y axis scale mode. Nexera X2 SPD-M30A...
  • Page 56 [1 nm]: Switch to the 1 nm slit. [Instrument] - [Slit] [8 nm]: Switch to the 8 nm slit. n Measurement Result Display Settings The settings related to the display of measurement results are as follows. Nexera X2 SPD-M30A...
  • Page 57 [Fixed Scale settings when [Scale Mode Y] - Settings] [Scale Mode Y] [Fixed Scale Set the fixed scale is a fixed scale. Settings] absorbance range (in units of mAbs) Absorbance] applied when an absorbance spectrum is displayed. Nexera X2 SPD-M30A...
  • Page 58 Zoom in on any section of the graph by dragging the mouse on the displayed spectrum to select an area for enlargement. Zooming operations including [Undo], [Redo], and [Initialize] are selected from the [View] menu (the same menu can be displayed by right- clicking the mouse on the displayed spectrum). Nexera X2 SPD-M30A...

  • Page 59: Measuring The Absorbance Spectrum

    Measure the sample absorbance. Feed the sample into the cell and then click (absorbance spectrum) or [Absorbance Spectrum] on the [Measure] menu. The absorbance is calculated from the spectrum and background spectrum when [Absorbance Spectrum] is executed and the resulting absorbance spectrum is displayed. Nexera X2 SPD-M30A...

  • Page 60: Checking The Absorbance Accuracy Correction Coefficient

    Launch SPD-M30A Utility. "5.3.1 Launching SPD-M30A Utility" P.38 Click (wavelength check) or (wavelength check/calibration). Click [Show Absorbance Accuracy correction coefficient] on the [Instrument] menu. The absorbance accuracy correction coefficient is displayed. Nexera X2 SPD-M30A...

  • Page 61: Enabling And Disabling The Correction Function For

    Linearity Correction] on the [Instrument] menu in the [Wavelength Check] window (Switching is not possible). Click [Show Linearity Correction] on the [Function] menu. Whether linearity correction is enabled or disabled is displayed. Administrator rights are required to change the [Linearity Correction] setting. Nexera X2 SPD-M30A...

  • Page 62: Setting Analysis Parameters

    Analysis parameters for analog output can be configured using SPD-M30A Utility. These analysis parameters are also applied to light intensity and absorbance measurements in SPD-M30A Utility. Launch SPD-M30A Utility. "5.3.1 Launching SPD-M30A Utility" P.38 Click (wavelength check) or (wavelength check/calibration). The [Wavelength Check] window is displayed. Nexera X2 SPD-M30A...
  • Page 63 5, 10, 25, 80, 160, 240, 320, 640, [Sampling] Set the sampling time. 1280, 2000 (ms) Set the time constant of the 0, 5, 10, 25, 80, 160, 240, 320, 640, [Time Constant] digital filter. 1280, 2000 (ms) Nexera X2 SPD-M30A...
  • Page 64 Enable or disable the [Reference Correction] ON, OFF reference wavelength function. The center of the wavelength [Ref. Wavelength] 190 to 700 (nm) used in reference subtraction. The bandwidth of the [Ref. Bandwidth] 0 to 100 (nm) reference wavelength. Nexera X2 SPD-M30A...

  • Page 65: Executing Analog Output Of Absorbance

    Click [Analog Output] - [Stop] on the [Measure] menu. Analysis will stop. n Zero Correction Click [Analog Output] - [Zero] on the [Measure] menu during analysis. The spectrum becomes the baseline of the analog output value (zero point) when [Zero] is clicked. Nexera X2 SPD-M30A...

  • Page 66: Checking The Instrument Temperature

    The [Temperature Check] window is displayed. Monitoring of the polychromator temperature starts and a graph of polychromator temperature is displayed. Start and stop polychromator temperature monitoring by clicking (polychromator temperature monitor) or [Monitor Polychromator Temperature] on the [Function] menu. Nexera X2 SPD-M30A...

  • Page 67: Configuring Network Settings (Spd-M30A Utility)

    "3.3.2 Configuring Network Settings (Web Browser)" P.15 Configuring the IP Address Launch SPD-M30A Utility. "5.3.1 Launching SPD-M30A Utility" P.38 Click [Network Settings] on the [Instrument] menu. The [Network Settings] window is displayed. Nexera X2 SPD-M30A...
  • Page 68 IP the IP address. address. System Information Settings Click [System Information Settings]. The [System Information Settings] window is displayed. The master server instrument list shows the SPD-M30A instruments on the network and system controllers available for connection. Nexera X2 SPD-M30A...
  • Page 69 [Group Name] field. Double-clicking on the system controller to be included in the parent-child relationship in the master server list will automatically enter the names for [Group Name] and [Master Name]. Nexera X2 SPD-M30A...
  • Page 70 Upgrade the system controller to the version The system controller compatible with the SPD-M30A. Even when is not compatible using this instrument in M20A compatibility with the SPD-M30A. mode, it is necessary to upgrade to the version compatible with the SPD-M30A. Nexera X2 SPD-M30A...

  • Page 71: Enabling/Disabling M20A Compatibility Mode

    Time constant 640, 1280, 640, 1280, in M20A compatibility mode. 2000 ms, 0 2000 ms, 0 (OFF) (OFF) Do not use M20A compatibility mode when using this instrument with LabSolutions. Launch SPD-M30A Utility. "5.3.1 Launching SPD-M30A Utility" P.38 Nexera X2 SPD-M30A...
  • Page 72 M20A compatibility mode. In this case, launching SPD-M30A Utility and connecting to an instrument in M20A compatibility mode temporarily disables M20A compatibility mode and adjustment and checking is performed in M30A mode. Exiting SPD- M30A Utility then returns the instrument to M20A compatibility mode. Nexera X2 SPD-M30A...

  • Page 73: Troubleshooting And Corrective Action

    • Check the error code number and Error indicator or liquid leak has error message and perform the P.70 lights up. indicated measures. occurred. *1 Refer to "2.2.1 Power Supply Connection" in the Nexera X2 System Guide. Nexera X2 SPD-M30A...
  • Page 74 • If bubbles are flowing through the cell and then flowing suddenly cell, perform corrective action for out. changing to bubbles*1. downward direction.) Nexera X2 SPD-M30A...
  • Page 75 • Use an appropriate barrier to block direct exposure of the instrument A strong draft from to drafts. an air conditioner, • Change the installation site. etc., is being drawn into the instrument. • Attach the provided insulation tube to the inlet plumbing. Nexera X2 SPD-M30A...
  • Page 76 In such a case, attach the provided insulation tube (S228-56760) to the inlet plumbing. This will insulate the plumbing between the oven and detector, reducing the baseline fluctuation due to ambient temperature change. Nexera X2 SPD-M30A...
  • Page 77 The exposure time • Perform automatic setting of the setting is not P.88 exposure time. appropriate. *1 Refer to "3.10.5 Light Source Usage Time Check" in the Nexera X2 Syatem Guide. Wavelength-Related Symptom Probable Cause Corrective Action Wavelength Wavelength is calibration has not Perform wavelength calibration.

  • Page 78: Error Message List

    D2 lamp is over 0x89C2 Lighting circuit current. malfunction There is a D2 lamp malfunction Replace the D2 lamp. P.82 problem with the D2 lamp. 0x89C3 Check the lamp Lighting circuit connections and malfunction the lamp for defects. Nexera X2 SPD-M30A...
  • Page 79 Malfunction inside the 0x8979 open. instrument A device with the same Change the IP address IP resource IP address as the of the instrument or 0x8956 contention. instrument exists on the device to resolve the same network. collision. Nexera X2 SPD-M30A...
  • Page 80 Replace the D2 lamp. P.82 D2 lamp 0x89D4 Malfunction inside the overvoltage. instrument D2 lamp malfunction Replace the D2 lamp. P.82 D2 lamp 0x89D5 Malfunction inside the overvoltage. instrument *1 Turn the power OFF and contact your Shimadzu representative. Nexera X2 SPD-M30A...

  • Page 81: Periodic Inspection And Maintenance

    Periodic Inspection and Maintenance Periodic inspections of this instrument must be performed to ensure safety during use. It is possible to have these periodic inspections performed by Shimadzu service personnel on a contractual basis. For information regarding a maintenance inspection contract, contact your Shimadzu representative.

  • Page 82: Periodic Inspection And Maintenance List

    Replace at about 2000 D2 lamp  hours of total P.82 replacement illumination time. 7.1.3 Check After Inspection and Maintenance After inspection and maintenance, check for any leakage during pumping. "6.1 Troubleshooting and Corrective Action" P.65 Nexera X2 SPD-M30A...

  • Page 83: Cleaning The Flow Cell

    Replacement part S228-56433-41 7.2.1 Removing the Flow Cell Do NOT disassemble the flow cell. The flow cell may be damaged and become unusable. Open the front cover. Loosen the coupling mounting screw and remove the coupling. Disconnect the connector. Nexera X2 SPD-M30A...

  • Page 84: Cleaning

    In such a case, use 2% formic acid solution for rinsing solution. Necessary Parts Part Name Type Part No. Syringe, 1ML Standard accessory S228-57028 Syringe needle Standard accessory S228-57027 Coupling 1.6-0.8C Standard accessory S228-40998-10 Male nuts 0.8MN-PEEK Standard accessory S228-46363 Male nuts ETFE Standard accessory S228-56759 Nexera X2 SPD-M30A...
  • Page 85 If an organic solvent is mixed with a buffer solution, crystals will form and clog the tubing. Insert the syringe needle on the tip of the syringe and turn it clockwise to fix it in place. Remove the tubing on the cell outlet tubing (with a coupling) end. Nexera X2 SPD-M30A...
  • Page 86 Gently push in the syringe's plunger. The 2-propanol will be injected into the cell interior and clean the cell. Flush the flow cell with distilled water or deionized water using a syringe to purge the rinsing solution. Nexera X2 SPD-M30A...

  • Page 87: Installing The Flow Cell

    Affix the coupling to the instrument using the coupling mounting screw. Connect the cell inlet tubing to the column and the cell outlet tubing to the waste container. When performing the above operation, do not let any air enter the flow lines. Reattach the front cover. Nexera X2 SPD-M30A...

  • Page 88: Cleaning For Alkaline Substances

    The delivery time of the formic acid depends on the degree of the contamination. In the case of heavy contamination, approx. 5 hours of pumping may be needed Rinse the flow cell with methanol for 2 hours at 1 mL/min. Nexera X2 SPD-M30A...

  • Page 89: Cleaning When The Light Intensity In The Ultraviolet Region Is Less Than 1 V

    When the flow cell should be stored, pump methanol into the flow cell once, either extract the methanol using a syringe and completely dry the cell interior or fill the cell interior with methanol and seal the cell tightly before storing the flow cell. Nexera X2 SPD-M30A...

  • Page 90: Replacing The Lamp

    • Be careful not to break the lamp. • Do not impart shocks or vibrations to the lamp. 7.4.1 Replacing the D2 Lamp Necessary Parts Part Name Type Part No. D2 lamp Consumable part S228-54515 Nexera X2 SPD-M30A...
  • Page 91 Loosen the lamp cover mounting screw and remove the lamp cover. Disconnect the connector. Loosen the two D2 lamp mounting screws and pull the lamp out of the instrument. Release the stopper by pinching the part indicated by arrows in the figure, and pull out the connector. Nexera X2 SPD-M30A...

  • Page 92: Resetting The Lamp Usage Time

    "7.4.2 Resetting the Lamp Usage Time" P.84 "7.6 Setting the Exposure Time" P.88 7.4.2 Resetting the Lamp Usage Time When the lamp is replaced or the exposure time is set, reset lamp usage time. Procedure Launch SPD-M30A Utility. "5.3.1 Launching SPD-M30A Utility" P.38 Nexera X2 SPD-M30A...
  • Page 93 Check the part number in [P/N] and click [OK]. The total lamp illumination time is reset. Part Name Part No. D2 lamp S228-54515 Reconfigure exposure time settings for all registered cells after replacing the lamp. "7.6 Setting the Exposure Time" P.88 Nexera X2 SPD-M30A...

  • Page 94: Preparation Before Using A Non-Standard Accessory Cell (Registration/Calibration)

    Not required value used. If different correction is required for each cell, Shimadzu service personnel will perform calibration. Perform the following tasks to utilize the cell ID function. Register a cell. Calibrate the registered cell.

  • Page 95: Cell Calibration

    Perform cell calibration for each registered cell. Set the exposure time. This eliminates the need to reconfigure exposure time settings every time the cell is replaced. "7.6 Setting the Exposure Time" P.88 Calibrate the wavelength. "7.7 Performing Wavelength Calibration" P.90 Nexera X2 SPD-M30A...

  • Page 96: Setting The Exposure Time

    Change the column to coupling 1.6C (P/N: S228-16004-13) and flush the flow cell with water from the solvent delivery pump. Visually confirm that there is no leakage. Launch SPD-M30A Utility. "5.3.1 Launching SPD-M30A Utility" P.38 Click (wavelength check/calibration). The [Wavelength Check/Calibration] window is displayed. Nexera X2 SPD-M30A...
  • Page 97 Clicking [Show Exposure Time] on the [Instrument] menu allows you to check the currently configured exposure time. Perform a lamp light intensity check. Refer to "3.10.7 Lamp Intensity Check" in the Nexera X2 System Guide. "5.3.2 Measuring Light Intensity" P.45 in this manual.

  • Page 98: Performing Wavelength Calibration

    Confirm that there is no leakage and that the output signal from the instrument is stable. If there are bubbles in the cell, the output signal will become unstable and calibration cannot be configured correctly. Launch SPD-M30A Utility. "5.3.1 Launching SPD-M30A Utility" P.38 Nexera X2 SPD-M30A...
  • Page 99 7.7 Performing Wavelength Calibration Click (wavelength check/Calibration). The [Wavelength Check/Calibration] window is displayed. Click (wavelength calibration) or [Wavelength Calibration] - [Execute] on the [Function] menu. Automatic wavelength calibration is executed and the calibration results are displayed. Nexera X2 SPD-M30A...

  • Page 100: Adjusting The Leak Sensor

    (wavelength check) or [Wavelength Check] - [Execute] on the [Function] menu. Judgment criterion: Error within 1 nm "3.10.6 Wavelength Accuracy Check" in the Nexera X2 System Guide Adjusting the Leak Sensor Perform leak sensor adjustment and check. During a certain period after turning ON power to the instrument (about 3 minutes), sensor output values cannot be obtained ("0"...
  • Page 101 Check that the area around the leak sensor is dry and then click [Calibrate to Zero]. The current sensor output value is calibrated to "100". After clicking [Calibrate to Zero], check that the fluctuation of [Leak Sensor Level (Current)] is in the range of 95 to 105. Nexera X2 SPD-M30A...
  • Page 102 If the [Leak Sensor Level (Current)] is significantly outside the range of 95 to 105 in the dry state, adjust the leak sensor. "7.8 Adjusting the Leak Sensor" P.92 Click [Start Check] in the [Leak Sensor Check/Calibrate] window to start the sensor check. Nexera X2 SPD-M30A...

  • Page 103: Replacing The Dust Filter

    If the dust filter becomes discolored from its original white, replace it with a new one. Be sure to turn OFF power to the instrument before replacing the filter. Nexera X2 SPD-M30A...

  • Page 104: Cleaning The Exterior

    Do NOT allow water to remain on the instrument surface and do NOT use alcohol or thinner-type solvents to clean the surfaces. These solvents can cause rusting and discoloration. Nexera X2 SPD-M30A...

  • Page 105: Technical Information

    Failure to do so may cause the instrument to ignite and cause a fire. Before performing wiring, turn OFF all components and unplug the power cables. Failure to do so may cause the instrument to ignite and cause a fire. Nexera X2 SPD-M30A...
  • Page 106 "8.1.2 External Input/Output Terminals" P.101 These are analog output connectors. [INTEGRATOR] connectors (four channels) "Connecting INTEGRATOR Connectors" P.100 Make connections to the above according to system requirements. The connection method for each connector or terminal is explained on the subsequent pages. Nexera X2 SPD-M30A...
  • Page 107 Acceptable Hub and Ethernet Type Part Name Remarks A 100 M switching hub is recommended. "1.4 Items Required for Operation" P.4 Category 5 UTP Ethernet network cable (straight-through cable) Ethernet cable "1.4 Items Required for Operation" P.4 Nexera X2 SPD-M30A...
  • Page 108 (integrator) and recorder. Wire as shown figure below. Insert the provided signal cable into an [INTEGRATOR] connector and connect the chromatopac as shown in the figure above. "1.5 Optional Parts" P.5 "4.2 Analog Output Usage and Settings" P.27 "5.3.6 Setting Analysis Parameters" P.54 Nexera X2 SPD-M30A...

  • Page 109: External Input/Output Terminals

    Connecting the External Input/Output Terminals External input/output terminals are used to input analysis start and stop signals and to output contact signals when errors are detected. Necessary Parts Part Name Type Part No. Event cable Standard accessory S228-28253-91 Nexera X2 SPD-M30A...

  • Page 110: Software Installation

    SPD-M30A Utility is installed to the PC at the same as LC workstation LabSolutions is installed. To use SPD-M30A Utility without using LabSolutions, insert the CD-ROM provided with the SPD-M30A and execute the "SPDM30Autility.exe" file on the CD-ROM. Nexera X2 SPD-M30A...

  • Page 111: Specifications

    "Precautions After Operation" P.12 I.D. 0.1 mm  length 450 mm Cell inlet tubing diameter I.D. 0.13 mm  length 230 mm Cell outlet tubing diameter Nexera X2 SPD-M30A...

  • Page 112: Detector Unit

    *1 Take note that the ambient temperature and humidity range for the entire system may be limited by the specifications of the PC and printer. *2 Connect the instrument to a power outlet that is equipped with a circuit breaker that shuts off the current at the described value or less. Nexera X2 SPD-M30A...

  • Page 113: Replacement Parts

    10 BASE-T (Full Duplex/Half Duplex) manual configuration speed 100 BASE-TX (Full Duplex/Half Duplex) possible Configuration possible Default IP 192.168.200.98 address using a web browser Port Web browser configuration screen Ports Communications with the PC 5006 (LabSolutions) Communications with the 5007 CBM-20A Nexera X2 SPD-M30A...
  • Page 114 8 Technical Information *1 See the following reference for changes to communication speed and IP address. "3.3 Configuring the System" P.14 Nexera X2 SPD-M30A...
  • Page 115 Cleaning the Flow Cell Grid [CLEAR] button Group Name 20, 61 Communication speed Component Parts Hg (mercury) lamp holder Condition of Consumable Parts [Configuration] tab Configuring Network Settings Connecting INTEGRATOR Connectors Indications Used in This Manual Connecting the Ethernet Connector Nexera X2 SPD-M30A...
  • Page 116 Names and Functions of Displays Replacement Parts Network Settings Replacing the D2 Lamp noise Replacing the Dust Filter Noise level Replacing the Lamp Non-Standard Accessory Cell Resetting the Lamp Usage Time response [RS-232C] connector Operation Log Operator Operator ID Nexera X2 SPD-M30A...
  • Page 117 Temperature fuse Temperature sensor Time constant 23, 55 Troubleshooting Tubing holder Turning the Power ON/OFF User ID 14, 20 [User Management] tab User Rights Utility Version display Voltage output range 27, 104 Wavelength Wavelength (Center) Wavelength (End) Nexera X2 SPD-M30A...
  • Page 118 This page is intentionally left blank.

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