Accuris Smart-Q 100 Operation Manual
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Accuris Smart-Q 100 Operation Manual

Fluorometer
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Operation Manual
V1.1
This Product is for Research Use Only

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Summary of Contents for Accuris Smart-Q 100

  • Page 1 Operation Manual V1.1 This Product is for Research Use Only...
  • Page 2 Please inspect the instrument as well as all included accessories when you first open the packaging. If you find anything damaged or missing, please contact Benchmark Scientific or your local distributor immediately. BENCHMARK SCIENTIFIC / ACCURIS INSTRUMENTS PO Box 709 Edison, NJ 08818 Phone: 908-769-5555 Website: www.benchmarkscientific.com / www.accuris-usa.com...
  • Page 3 Safety Warning and Guidelines 1. Important information for safe use Users should have a clear understanding of how to use this instrument before operation, please read this manual carefully prior to operation. NOTICE: Please review this manual in its entirety before operating the Smart-Q™...
  • Page 4 Unplug the instrument immediately if any of the following occur, and contact Benchmark Scientific’s Service Department: ⚫ Liquid enters the Instrument. ⚫ The instrument is dropped or the outer shell damaged. ⚫ The functions of the instrument have obviously changed. 3.

  • Page 5: Table Of Contents

    Table of Contents Chapter 1 Introduction ......................1 Chapter 2 Specifications ......................2 1. Required Installation Environment ................2 2. Specifications ....................... 2 Chapter 3 Instrument Overview ..................... 3 Chapter 4 Installation ......................4 Initial Check ......................4 Installation Conditions ..................4 Operating Conditions ...................
  • Page 6 5.9.1 System Settings Interface .................. 18 5.9.2 Upgrade Interface ....................19 5.9.3 Maintenance Interface ..................19 Chapter 6 Troubleshooting ....................20...

  • Page 7: Chapter 1 Introduction

    Thus, unknown samples can be quantitatively analyzed. Reagent kits with fluorescent dye, buffer, and standards are available from Accuris or your local distributor. Key Features and Benefits: ➢...

  • Page 8: Chapter 2 Specifications

    Smart-Q 100 Fluorometer Operation Manual Chapter 2 Specifications Chapter 2 Specifications 1. Required Installation Environment Environmental Temperature: 10°C～40°C Relative Humidity: ≤ 70% Input Voltage: 24 VDC 2A 2. Specifications Model Smart-Q™ 100 Fluorometer Display 7.0-inch, color touch screen Dynamic Range...

  • Page 9: Chapter 3 Instrument Overview

    Smart-Q 100 Fluorometer Operation Manual Chapter 3 Instrument Structure Chapter 3 Instrument Overview This chapter provides an overview of the physical characteristics of the Smart-Q 100. When using the instrument for the first time, please read this chapter carefully to familiarize yourself with all the parts.

  • Page 10: Chapter 4 Installation

    Each Smart-Q 100 has been factory-inspected prior to shipping. Please refer to the included packing list to ensure that all items are included. Contact Accuris or your local distributor if there are any signs of damage to the packaging upon receipt.
  • Page 11 Smart-Q 100 Fluorometer Operation Manual Chapter 4 Installation Fluorescent PCR tube Fig 4.3.01 Instrument Operation Diagram...

  • Page 12: Chapter 5 Software Introduction

    Smart-Q 100 Fluorometer Operation Manual Chapter 5 Software Introduction Chapter 5 Software Introduction 5.1 Instrument Startup Power the instrument on. Upon start-up, the instrument will perform a self-check (Fig. 5.1.01). After the self-check, the Login Interface will be displayed (Fig. 5.1.02).

  • Page 13: Dsdna Interface

    Smart-Q 100 Fluorometer Operation Manual Chapter 5 Software Introduction Table 5-1 Main Menu Interface Operations Name Function Select to manage account settings or to switch between users. Select to view the working solution calculator Select to enter the dsDNA Interface...

  • Page 14: Dsdna Interface

    Smart-Q 100 Fluorometer Operation Manual Chapter 5 Software Introduction Fig 5.3.01 dsDNA test type Fig 5.3.02 dsDNA test 5.3.1 dsDNA Interface Select the test type and click “OK” to enter next interface. The following takes dsDNA: High Sensitivity as an example.

  • Page 15: Calibration Curve Interface

    Smart-Q 100 Fluorometer Operation Manual Chapter 5 Software Introduction 5.3.2 Calibration Curve Interface Prior to sample measurements, a calibration curve must be created using the standards that are typically included with a quantification kit. Only 2 standards are required to form the calibration curve.

  • Page 16: Molarity Calculator

    Smart-Q 100 Fluorometer Operation Manual Chapter 5 Software Introduction Fig 5.3.06 Testing interface Fig 5.3.07 Test Report Fig 5.3.08 Modify sample name : Delete selected test results. : Export all result of current testing. : Print all result of current testing.

  • Page 17: Normalization Calculator

    Smart-Q 100 Fluorometer Operation Manual Chapter 5 Software Introduction Select the conversion units: ng/μL, ng/mL, μg/μL, μg/mL, mg/mL. After selecting the conversion units, select the units: M, mM, μM, nM, pM. Input the molecular weight; the default value for dsDNA is set to 660g/mol.

  • Page 18: Fluorescence Interface

    Smart-Q 100 Fluorometer Operation Manual Chapter 5 Software Introduction concentration after dilution will be displayed (Fig. 5.3.12). The Initial Conc. interface shows the concentration of the original sample (Fig. 5.3.13). Click “Export” to export current data to an inserted USB drive.

  • Page 19: Working Solution Calculation Settings

    Smart-Q 100 Fluorometer Operation Manual Chapter 5 Software Introduction Test Types: Blue 470nm and Red 635nm are single excitation wavelength tests, whereas Blue & Red is a dual-wavelength test (Fig 5.4.03). The Blue 470nm interface is identical to the Red 635nm interface. Click to begin a sample measurement.

  • Page 20: Standard Curve Interface

    Smart-Q 100 Fluorometer Operation Manual Chapter 5 Software Introduction 5.6 Standard Curve Interface From the Main Menu Interface, click to enter the Standard Curve Interface (Fig. 5.6.01). Fig 5.6.01 Standard Curve Interface : Select the sample type (dsDNA, RNA, Oligo, Protein).
  • Page 21 Smart-Q 100 Fluorometer Operation Manual Chapter 5 Software Introduction Fig 5.6.02 New Curve Selection Box Fig 5.6.03 New Standard Curve Interface : Highlight the previous standard. : Highlight the next standard, or to add an additional standard. : View Parameter Settings (EX / EM wavelengths + concentration units).

  • Page 22: Curve Management

    Smart-Q 100 Fluorometer Operation Manual Chapter 5 Software Introduction 5.6.2 Curve Management From the Standard Curve Interface Click to enter Curve Management Interface (Fig. 5.6.04). Fig 5.6.04 Curve Management Interface Fig 5.6.05 Importing Curve(s) : Delete the selected curve. : Export the selected curve(s) to a USB drive (Fig 5.6.04).

  • Page 23: User Management Interface

    Smart-Q 100 Fluorometer Operation Manual Chapter 5 Software Introduction Click on a report to view its saved data (Fig. 5.7.03). Click on the sample from the row to view the detailed sample report (Fig. 5.7.04). Fig 5.7.03 Saved Report Data Fig 5.7.04 Detailed Sample Report...

  • Page 24: Settings Interface

    Smart-Q 100 Fluorometer Operation Manual Chapter 5 Software Introduction 5.9 Settings Interface Click in the main interface to enter the Settings Interface (Fig. 5.9.01). Fig 5.9.01 Settings Interface System Settings Upgrade Maintenance 5.9.1 System Settings Interface Fig 5.9.02 System Settings Click to enter system settings (Fig.

  • Page 25: Upgrade Interface

    Fig 5.9.03 Upgrade interface 5.9.3 Maintenance Interface From the Main Menu Interface, click and input the password to enter the Maintenance Interface. This is only available for authorized service procedures. Contact Accuris Instruments or your local distributor for details.

  • Page 26: Chapter 6 Troubleshooting

    Check the power; Check whether the module is loose, if loose Light source not Light source failure; Contact Accuris visible Module poor contact Instrument or your local distributor Measurement not PCR tube isn’t in Check the PCR tube and...

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F1001

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