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Hitachi F-4700 Instruction Manual

Hitachi F-4700 Instruction Manual

Fluorescence spectrophotometer
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INSTRUCTION MANUAL
5J2-9015-000 Ver.1 September. 2017
Fluorescence Spectrophotometer
HITACHI F-4700
FLUORESCENCE
SPECTROPHOTOMETER
(MAINTENANCE
MANUAL)

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  • Page 1 INSTRUCTION MANUAL 5J2-9015-000 Ver.1 September. 2017 Fluorescence Spectrophotometer HITACHI F-4700 FLUORESCENCE SPECTROPHOTOMETER (MAINTENANCE MANUAL)
  • Page 2 Hitachi High-Tech Science Corporation. 3. Hitachi High-Tech Science Corporation assumes no liability for any direct, indirect, or consequential damages arising from use not described in this manual.
  • Page 3 FOREWORD  This instruction manual describes the handling of F-4700 for its users.  The F-4700 fluorescence spectrophotometer is intended for fluorescence analysis, and should not be used for other purposes.  This instrument is aimed for personnel trained in basic chemical analysis.

  • Page 4: Preface

    Note that samples that may have been infected with bacteria or viruses are not applicable to the instrument. The F-4700 is designed for use by personnel trained in and acquainted with chemical analyses. Be aware that appropriate handling of analytical equipment, reagents, or samples is essential for obtaining proper results and preventing safety-hazardous situations.

  • Page 5: Important

    (2) In other Countries outside the EU If you wish to discard this product, please contact your local authorities and ask for the correct method of disposal. Instruction Manual for F-4700 Cautions The following is a statement of notice about EMC for Korea.

  • Page 6: Warranty On Product

    Warranty on Product The Hitachi F-4700 Fluorescence Spectrophotometer is warranted to be free from defects in material or workmanship under normal use within the product specifications indicated in this manual and under conditions given below. This warranty is void if the instrument is not used in accordance with the instruction manual.
  • Page 7 (f) Failure due to improper handling or maintenance by the user. (g) Failure due to maintenance or repair by a service agent not approved or authorized by us. (h) Failure due to relocation or transport after the initial installation. Failure due to disassembly, modification or relocation not approved by Failure due to acts of God, including fire, earthquake, storm, flood, lightning, social disturbance, riot, crime, terrorism, war (declared or undeclared), radioactive pollution, contamination with harmful...

  • Page 8: Service Life Of This Instrument

    (8 h/day, 20 /month)) For using the instrument beyond the useful service life, it shall be checked for safety by Hitachi High-Technologies Corporation sales representative or service office of Hitachi High-Technologies Corporation sales representative. (This safety check will be available on a chargeable basis.) If use of the instrument is continued without receiving the safety check, the instrument might become faulty and cause a danger.

  • Page 9: Other Precautions

    (c) The maintenance and consumables of the instrument can be supplied within the useful service life of the instrument (7 years). Even after the period of useful service life, the parts and units can be supplied (within 10 years after the date of initial use) so far as they are obtainable. However, this measure will not lead to an extension of the 7-year useful service life which is assured by the manufacturer.

  • Page 10: Safety Summary

    SAFETY SUMMARY Precautions on Safety Before using the F-4700 fluorescence spectrophotometer, be sure to read through and understand the safety instructions given below. The hazard warnings that appear on the warning labels on the product or in the manual have one of the following alert headings consisting of an alert symbol and signal word DANGER, WARNING or CAUTION.

  • Page 11: General Safety Guidelines

     Do not perform any operation or action other than described in this manual. When in doubt, please contact your nearest Hitachi High-Technologies Corporation sales representative or service office of Hitachi High-Technologies Corporation sales representative.
  • Page 12 Hitachi High-Technologies Corporation sales representative or service office of Hitachi High-Technologies Corporation sales representative. SAFETY - 3...
  • Page 13 If any components are missing or damaged or if you have any question, consult the installation engineer, or the dealer from whom you purchased the instrument, or your nearest Hitachi High-Technologies Corporation sales representative or service office of Hitachi High-Technologies Corporation sales representative.
  • Page 14 For other than the replacement procedures instructed in this manual, contact your nearest Hitachi High-Technologies Corporation sales representative or service office of Hitachi High-Technologies Corporation sales representative.  For reducing a risk of trouble occurrence due to physical deterioration, it...

  • Page 15: Warning Indications

    SAFETY SUMMARY WARNING Indications Electric Shock upon Contact with Instrument Interior  An electric shock caused by contact with the power supply (30 kV) of the xenon lamp could result in fatal or serious injury. Be sure to turn off the power switch before removing the light source cover for replacing the lamp.
  • Page 16 SAFETY SUMMARY WARNING Indications (Continued) Injury due to Xenon Lamp Explosion [During use] When the cumulative turn-on time of the xenon lamp reaches the limit for use, vapor and dispersed particles from the electrodes will adhere to the lamp wall and darken it, causing a decrease in heat dissipation.
  • Page 17 SAFETY SUMMARY WARNING Indications (Continued) (5) Be sure to wear proper protective gear such as safety goggles, safety mask, thick long sleeves, and gloves when handling the xenon lamp. Do not touch the quartz glass part of the lamp with bare hands.
  • Page 18 SAFETY SUMMARY WARNING Indications (Continued) [At disposal] For disposal of the used xenon lamp, wrap it in a thick cloth (such as triple-folded cotton cloth) completely and crush its glass part with a hammer or the like. Then discard the lamp properly as a hazardous waste item. (Section 4.4) SAFETY - 9...

  • Page 19: Caution Indications

    SAFETY SUMMARY CAUTION Indications Touching Hot Part Could Result in Burns The xenon lamp and its housing become very hot during operation and remain hot for a while after operation. Touching the lamp or housing in this condition could result in severe burns. Before proceeding to lamp replacement, turn off the power switch and wait for at least one hour until the lamp and its housing cool down sufficiently.
  • Page 20 SAFETY SUMMARY CAUTION Indications (Continued) Prevent Tipover of Instrument Personal injury could be incurred if the instrument tips over. When installing the instrument, secure it properly to prevent tipover. (Section 1.1.1(3)) SAFETY - 11...

  • Page 21: Warning Labels

    SAFETY SUMMARY Warning Labels The warning labels indicated below are attached to the F-4700 fluorescence spectrophotometer. SAFETY - 12...

  • Page 22: Table Of Contents

    CONTENTS FOREWORD PREFACE ............................... 1 ABOUT THIS MANUAL ........................... 1 IMPORTANT ........................IMPORTANT-1 Information for Users on WEEE (only for EU Countries) ......... IMPORTANT-1 Warranty on Product ....................IMPORTANT-2 Service Life of This Instrument ................. IMPORTANT-4 Installation, Relocation and After-sale Technical Service ........IMPORTANT-4 Technical Seminars and Training Courses for Users..........
  • Page 23 1.5.2 Cord Connection ...................... 1-13 1.5.3 Printer Connection ....................1-14 Installation of FL Solutions Program (Windows 7) ..............1-15 1.6.1 Specifications of Personal Computer ............... 1-15 1.6.2 Setup of FL Solutions Program ................1-16 1.6.3 Reinstallation of This Program ................. 1-18 1.6.4 Setup of USB Driver ....................
  • Page 24 Usage Time of Xe Lamp ....................... 3-19 Cleaning of Lens in Sample Compartment ................3-20 Cleaning of Cell Holder in Sample Compartment ..............3-21 Troubleshooting ........................3-24 3.6.1 Troubleshooting Procedure ..................3-24 3.6.2 Error Message List ....................3-26 REPLACEMENT PARTS......................... 4-1 Consumables and Spare Parts .....................

  • Page 25: Installation (For Customer's Reference)

    1. INSTALLATION (For customer’s reference) The customer should not attempt to install the spectrophotometer at delivery. To enable safe and accurate use of the instrument, the installation will be carried out by our service agent or technicians who have been trained and approved by us. Prior to installation, the customer must prepare the installation requirements for the instrument with reference to this section.
  • Page 26 Antiseismic Provision Two threaded holes are provided in the base at the rear of the instrument to enable securing it. Use these holes for securing the instrument if necessary. (The threaded holes are for 4 mm screws, and the spacing between them is 100 mm.) Fig.

  • Page 27: Environment Requirements

    1.1.2 Environment Requirements Operating Temperature Range: 15 to 35 C To allow measurement in the most stable conditions, it is recommended to install the instrument in a room air-conditioned at 20 to 25 C. Operating Humidity Range: 20 to 80% Storage Temperature Range: -20 to 60 C Ambient Gas The room should be free from acidic, alkaline and other gases that may significantly corrode...

  • Page 28: Power Requirement

    NOTICE: The optical system is very delicate. The control system incorporates parts constructing a high-density electronic circuit that functions as a computer. Careful consideration should thus be given to the aforementioned items. 1.1.3 Power Requirement Power Supply Voltage Rated voltage: 100, 115, 220, 230 or 240 V AC Allowable fluctuation is within 10% of the rated voltage.
  • Page 29 WARNING Electric Shock due to Improper Grounding  Be sure to use the grounded 3P power cord supplied with the instrument. The use of a power cord other than the specified one could result in an electric shock hazard.  Connect the 3P power cord to a grounded 3P power outlet.

  • Page 30: Unpacking

    This instrument weighs as much as 41 kg. When carrying it, exercise care not to drop it and incur injury. Two persons should hold the left and right sides of the instrument securely when moving it. Spectrophotometer Data processor main unit (personal computer) Fig. 1-5 The F-4700 Fluorescence Spectrophotometer 1 - 6...

  • Page 31: Check Of Delivered Items

    1.3 Check of Delivered Items Upon completion of unpacking, check the delivered contents against the packing list in the presence of the customer. If any item is missing or damaged or if you have any question, then contact the dealer from whom you purchased the instrument or our nearest sales representative.
  • Page 32 Knobs Fuse holder Power connector Fig. 1-6 Fuse Holder Location 1 - 8...

  • Page 33: Assembly

    1.5 Assembly WARNING Contact with the power supply voltage could cause an electric shock, resulting in fatal or serious injury. Before starting assembly, make sure that the power switch of the spectrophotometer main unit is OFF and the power cord is disconnected. 1.5.1 Attachment of Xenon Lamp Detach the cover from the light source compartment on the left side of the instrument.
  • Page 34 WARNING Explosion of the xenon lamp could cause serious injury. High-pressure gas of about 1 MPa (at room temperature) is sealed in the xenon lamp. Be careful not to apply pressure to the lamp bulb (glass part) when replacing the lamp. Also be sure to wear proper protective gear such as safety goggles, thick long sleeves, and gloves when handling the lamp.
  • Page 35 ③ Grasp top of lamp holder then lift up and pull it out. Fig. 1-9 Removal of Lamp Holder (2) Mount the xenon lamp in the lamp holder according to Figs. 1-10 and 1-11. Lamp holder Electrodes Xenon lamp Nuts supplied with xenon lamp Fig.
  • Page 36 Cathode cord Anode cord Anode (+) side Cathode (-) side (with mark) Mount lamp so that projection is located as shown in figure. Fig. 1-11 Mounting of Xenon Lamp Secure the anode and cathode nuts so they will not loosen. Attach the lamp holder to the instrument by reversing the foregoing steps (2) to (4).

  • Page 37: Cord Connection

    1.5.2 Cord Connection Connect cords as indicated in Fig. 1-12. WARNING Contact with the power supply voltage could cause an electric shock, resulting in fatal or serious injury. Before starting cord connection, make sure that the power switch of the spectrophotometer main unit is OFF. To AC power supply Printer cable Printer...

  • Page 38: Printer Connection

    1.5.3 Printer Connection Be sure to use a printer that is compatible with each OS. The method of connection varies with the printer model. Make connection with reference to the instruction manual provided with your printer. NOTE: A printer and printer cable must be prepared on separate order. 1 - 14...

  • Page 39: Installation Of Fl Solutions Program (Windows 7)

    1.6 Installation of FL Solutions Program (Windows 10) 1.6.1 Specifications of Personal Computer For running the FL Solutions 4.2 program, the following specifications must be met. Microsoft ® Windows 10 Professional (64bit) CPU that allows the above OS to run in your computer Main memory : Minimum 4 GB Hard disk...

  • Page 40: Setup Of Fl Solutions Program

    1.6.2 Setup of FL Solutions Program (for Windows 10) For installing this program, implement the following procedure. Set the accompanying CD in the CD-ROM drive.  A window as shown in Fig. 1-13 will appear. If not, click [Start]-[Computer] and select the CD-ROM drive.
  • Page 41 Click Next.  A window as shown in Fig. 1-16 will appear. Fig. 1-15 Select the installation destination and click Next. Installation starts.  Upon completion of installation, a window as shown in Fig. 1-17 appears. Fig. 1-16 For placing the shortcut of this program on the desktop, put the check mark in this check box.

  • Page 42: Reinstallation Of This Program

    1.6.7 Reinstallation of This Program (for Windows 10) Before reinstallation, the existing program needs to be deleted. Carry out the following procedure for deleting the program. Select [Start]-[Setting]-[Apps& features] and a window as shown in Fig. 1-18 will appear. Select “FL Solutions 4.2” and click “Uninstall”.

  • Page 43: Setup Of Usb Driver

    After system setup for FL Solutions, the USB driver needs to be set up for communication with the Model F-4700. Connect a USB communication cable and turn on the power switch of F-4700 Click the Windows start button and activate [setting] Go to the system, then click [connected devices].
  • Page 44 The dialog “Update Driver Software” appears. Click “Browse my computer for driver software”. Fig. 1-21 The dialog “Update Driver Software” appears. Click Browse. Fig. 1-22 Specify the directory in which FL Solutions have been set up. * Directory Windows (64bit) C: \FL Solutions\Drivers\USBx64 Click OK.
  • Page 45 Location：” and click Next. Fig. 1-24 The Windows security message appears. Check “Always trust software from “Hitachi High-Tech Science Corporation”” and click “Install”. Fig. 1-25 The installation of the driver software begins. After completion of installation, “Windows has successfully updated your driver software”...

  • Page 46: Startup Of Fl Solutions Program

    1.7 Startup of FL Solutions Program To start up the FL Solutions program, carry out the following procedure. Make sure the standard- equipped communication cable (USB cable, sold separately) is connected between the fluorescence spectrophotometer and the personal computer (PC). Turn on the PC power supply and wait until Windows 7 starts.
  • Page 47 (2) The other method begins with clicking the start button at the bottom left corner of desktop. Next, point to All Programs (P) and Hitachi FL Spectrophotometer, and then select FL Solutions 4.2. Fig. 1-29 The computer now recognizes the fluorescence...
  • Page 48  Wavelength drive system communication port or its power is not turned on,  Xenon lamp turn-on the monitor window (“F-4700 Fluorescence Spectrophotometer on USB” indicated on its title If an abnormality is found in the bar) is not presented. In this status, functions for self-diagnosis, the relevant error other than measurement are usable.
  • Page 49 After the Model name is selected, a message as in Fig.1-33 will appear. Click the OK button. Fig. 1-33 A [Setup WL Calibration data] window as in Fig. 1-34 will now appear. Enter the values shown on the wavelength calibration value label (⑨ in Fig.
  • Page 50 On completion of initialization, the window shown in Fig. 1-36 is displayed. When “Ready” appears, measurement can be started. Fig. 1-36 NOTE: A sample should be set after “Ready” appears. If the lid of sample compartment is opened or closed in the “Initializing” or “Parameter” status, zero point may not be set exactly.

  • Page 51: Termination Of Fl Solutions Program

    1.8 Termination of FL Solutions Program After completion of measurement, shut down the instrument in the following procedure. From the File menu, select the Exit command. A window appears as shown below. Fig. 1-39 For termination without turning off the light source lamp, select “Close the monitor window, but keep the lamp operating?”.

  • Page 52: Checkup After Installation And Assembly

    1.9 Checkup After Installation and Assembly Installation and assembly of the instrument have been completed according to the foregoing explanation. Now check the following items once again. Is there anything abnormal at the installation site? Has the xenon lamp been mounted correctly? Check the polarity in particular ((+) side should face up).

  • Page 53: Adjustment Of Light Source Position

    1.10 Adjustment of Light Source Position Carry out positional adjustment of the xenon lamp with reference to section 3.1 herein. First conduct a coarse adjustment visually, and then make a fine adjustment utilizing distilled water as a sample. 1.11 Check of Performance Refer to section 3.2 herein for a performance check.

  • Page 54: Functions

    2. FUNCTIONS 2.1 Instrument Configuration This instrument consists of a spectrophotometer main unit and a data processor. Figure 2-1 illustrates the configuration. Data processor (PC) Mouse Spectrophotometer Keyboard main unit Fig. 2-1 The F-4700 FL Spectrophotometer 2 - 1...

  • Page 55: Names And Functions Of Components In Spectrophotometer Main Unit

    2.2 Names and Functions of Components in Spectrophotometer Main Unit A description of the component names and functions follows. Sample compartment cover grip RUN indicator lamp Xe LAMP ignition indicator lamp Fig. 2-2 Front of Spectrophotometer  Sample compartment cover grip Pull up on this grip to open the sample compartment cover.
  • Page 56 Light source air-cooling louvers Xe lamp positioning screws Round lid for accessory Light source compartment cover Hole for admitting light for wavelength accuracy check Fig. 2-3 Left Side of Spectrophotometer  Light source compartment cover Remove this cover for replacing the Xe lamp. A cutoff switch is equipped for preventing ignition of the lamp when the cover is removed.
  • Page 57 Power fuse holder POWER switch Grounding terminal Power connector Fig. 2-4 Power Supply Section of Spectrophotometer  POWER switch Power switch for the spectrophotometer main unit. Power is supplied to the main unit by turning the switch ON. (I) mark indicates ON, and (O) indicates OFF. ...
  • Page 58 Retaining screw USB cable External signal terminal cover Fig. 2-5 Right Side of Spectrophotometer  External signal terminal cover Remove this cover and you can see various electrical signal connectors. External devices can be connected here for use. The cover is retained by one screw; remove the screw and cover for using the terminals.
  • Page 59 Communication External start D/A output port connector signal port Fig. 2-6 External Signal Terminal Section  Communication connector (PC) Connector for communication with a personal computer. Be sure to connect here the dedicated USB cable supplied with the instrument.  D/A output port (D/A) Connector for an analog output interface.
  • Page 60  EX OUT Terminal for sending timing signal to externally connected device when the spectrophotometer starts measurement. Output is provided only in wavelength scan, time scan, quantitative, fluorescence polarization, and intracellular calcium measurement modes.  BUSY Synchronization contact for sending operating condition of spectrophotometer to external device. At measurement : Closed Other than above :...
  • Page 61 Excitation side lens Sample cell holder Emission side lens Retaining screw Fig. 2-8 Sample Compartment Interior  Sample cell holder Set a fluorescence cell in the holder for measurement. The cell holder is fixed by one screw, and is readily detachable. ...

  • Page 62: Operating Principle

    2.3 Operating Principle 2.3.1 Signal Processing and Control System Figure 2-8 illustrates the signal processing and control system. The light emitted from the Xe lamp enters the excitation side monochromator, and part of the light from this monochromator is directed to the monitor detector by the beam splitter.

  • Page 63: Optical System

    2.3.2 Optical System The optical system of the F-4700 is shown in Fig. 2-10. The light emitted from the Xe lamp is condensed on entrance slit S1 of the excitation side monochromator via lenses L1 and L2. The light (excitation light) separated by EX side diffraction grating enters exit slit S2.

  • Page 64: Measurement Functions

     Quantitative calculation  Three-dimensional measurement  Three-dimensional time scan measurement 2.4.2 Automatic Calibration and Self-diagnosis Functions The following calibration and diagnosis functions are available in the F-4700.  Memory (RAM) check  Wavelength drive mechanism check  Chopper drive mechanism check ...

  • Page 65: Specifications

    2.5 Specifications Table 2-1 lists the specifications of the F-4700. Table 2-1 Specifications Light source 150 W Xe lamp, with self-deozonating lamp house Monochromator Provided on both excitation and emission sides Large-bore aberration-free concave diffraction grating of 900 lines/mm Blaze wavelength; EX side 300 nm, EM side 400 nm...

  • Page 66: Maintenance And Inspection

    3. MAINTENANCE AND INSPECTION The fluorescence spectrophotometer requires maintenance in order to keep it in proper running condition. This chapter explains the procedures for positional adjustment of the light source, check of performance, maintenance & inspection, and troubleshooting. 3.1 Positional Adjustment of Light Source Carry out the following adjustment when the Xe lamp has been replaced, when the lamp holder has been detached, or when the lamp positioning screw has been manipulated.
  • Page 67 A window as in Fig. 3-1 will open. Set the EX wavelength at 546 nm. Fig. 3-1 Place a sheet of white paper in front of the EX light exit lens, and adjust while watching the light beam shape. Excitation light (Excitation) exit window White...
  • Page 68 Using the supplied stubby screwdriver, turn any one of VERTICAL ADJ., HORIZONTAL ADJ. and FOCUS on the left side of the main unit and a green light will appear. Adjust so that the light beam shape on the excitation light exit window will resemble the shape of the EX lens light beam shown in Fig.

  • Page 69: Adjustment Of Xe Lamp Position Using Distilled Water (Fine Adjustment)

    Adjust with HORIZONTAL ADJ. so the light at the cell inlet will come to the center of the cell holder window. Cell inlet Cell holder Light beam Cell holder window Fig. 3-5 This completes the coarse adjustment of the Xe lamp position. Now proceed to fine adjustment of the lamp position.
  • Page 70 Fill the supplied quartz fluorescence cell with distilled water and set it in the cell holder in the sample compartment. After the above setting, click OK button. Click (start measurement) button on the measurement toolbar. The time scan measurement will start. Now adjust VERTICAL ADJ. and FOCUS on the left side of the main unit, using the supplied stubby screwdriver, so the values on the graph will become maximum.

  • Page 71: Check Of Performance

    3.2 Check of Performance 3.2.1 Check of EM Wavelength Accuracy Utilizing the Hg bright lines included in a fluorescent lamp located in the room where the instrument is installed, measure the spectrum in the vicinity of the bright lines and check the difference between the wavelength of bright lines and the wavelength indication.
  • Page 72 Unplug the power cord from the main unit connector (see Fig. 3-6). The light source compartment cover cannot be removed with the cord inserted in the connector. Fig. 3-6 Detachment of Power Cord Remove the two screws holding the light source compartment cover on the left side of the main unit (①, ②...
  • Page 73 Be sure to put on a safety mask, thick long sleeves, gloves, etc. prior to the removal procedure. Turn by hand and remove the high voltage terminal terminal screw on the anode cord side (③ in Fig. 3-8). An insulating stopper is attached to this terminal screw so it will not drop off and be lost. Turn by hand and remove the high voltage terminal terminal screw on the cathode cord side (④...
  • Page 74 Hold the lamp holder as shown in Fig. 3-10, and while grasping the screw part of the holder, lift the whole unit a little upward and detach it. Be careful when removing so the cathode side of the lamp will not hit the lower base. Fig.
  • Page 75 (12) Insert the power cord into the connector on the main unit. Then start up the spectrophotometer with reference to startup of the FL Solutions 2.1 program in section 1.10. (13) Set the supplied white paper for WL check at a slant in the cell holder of the sample compartment (Fig.
  • Page 76 (15) Click the (Pre Scan) button on the measurement toolbar. A pre-scan will thus be executed. (16) Click the (start measurement) button on the measurement toolbar. (17) When a spectrum is acquired, carry out peak detection and determine the peak wavelength. Make sure it is within the specification.

  • Page 77: Check Of Ex Wavelength Accuracy

    3.2.2 Check of EX Wavelength Accuracy Take the following procedure for the check. (analytical method) button on the measurement toolbar and set “wavelength scan” for Click the measuring mode under the [General] tab on the Method window. Then select the [Instrument] tab and set the conditions given below.
  • Page 78 Close the FL Solutions program (see section 1.11). Turn OFF the POWER switch of the spectrophotometer main unit. Unplug the power cord from the main unit connector. Detach the cover from the light source compartment and close the lid over the hole at the reverse of the light source compartment cover.

  • Page 79: Check Of Sensitivity

    3.2.3 Check of Sensitivity Check the signal-to-noise ratio on a Raman spectrum of water (Raman scattering) under fixed measurement conditions. For this sensitivity check, be sure to use a clean fluorescence cell containing fresh distilled water. The procedure is as follows. To ensure stability of the Xe lamp, wait at least 30 minutes after turning on the lamp.
  • Page 80 [Reference] Auto parameter setting (fixed)  <Sensitivity> Wavelength scan (acquisition of signal intensity)  EX and EM Slits : Both 10 nm  EX WL 350 nm  EM WL Raman peak wavelength  Response  Scan speed 60 nm/min ...
  • Page 81 Time Scan  EX and EM Slits : Both 10 nm  EX WL 350 nm  EM WL 420 nm  Response  Scan Time 601 s  PMT Voltage 700 V Calculation method   Calculation of the noise level (N) with the peak to peak definition Determine the peak position and photometric value (“S”...
  • Page 82 <Calculation method>  Height of Raman peak (S)  Noise level (N) 10 s 10 s 10 s … N = average value of N Peak wavelength Baseline Fig. 3-14 Check of Sensitivity Specifications: S/N 15000 or more (RMS) [BG] S/N 1000 or more (RMS) S/N 300 or more (Peak to Peak) 3 - 17...

  • Page 83: Stability

    3.2.4 Stability Determine the variation with time of emission intensity (including scattered light) under fixed measurement conditions. Conduct the measurement simultaneously with that in 3.2.3. The results will be indicated as in Fig. 3-13. Auto parameter setting (fixed)  Time scan mode ...

  • Page 84: Usage Time Of Xe Lamp

    3.3 Usage Time of Xe Lamp The cumulative ON time of the Xe lamp in the instrument can be monitored. For this purpose, select [Lamp Usage] under [Utility] on the menu bar. Fig. 3-15 Usage Time of Lamp Refer to section 4.3 for lamp exchange. When the lamp has been exchanged, click Reset Xe Lamp. When the window as shown in Fig.
  • Page 85 3 - 20...

  • Page 86: Cleaning Of Lens In Sample Compartment

    3.4 Cleaning of Lens in Sample Compartment Two types of quartz lenses are utilized in the sample compartment; excitation and emission side lenses (Fig. 3-18). Dust adhering to the lenses can be removed by wiping them carefully with a cleaning cloth moistened with a 1:1 mixture of ethanol and ethyl ether (also wear clean gloves for this purpose).

  • Page 87: Cleaning Of Cell Holder In Sample Compartment

    3.5 Cleaning of Cell Holder in Sample Compartment If sample is spilt in the sample compartment, clean the inside of the compartment if necessary by removing the cell holder and cell holder base. Following is the procedure for this. Open the top cover of the sample compartment. Figure 3-19 shows a top view of the sample compartment interior.
  • Page 88 Pull out the cell holder base toward you by grasping it as shown in Fig. 3-20. Fig. 3-20 To remove the lower base, detach the three lower base retaining screws. Avoid loosening the other screws (two screws nearest you). Lower base retaining screws Guide pin Lower base...
  • Page 89 Grasp the lower base as shown in Fig. 3-22. Insert your right hand through the round hole in the lower base, lift up slightly and detach the lower base from the two guide pins (the base fits rather snugly onto the guide pins). When detached from the pins, pull toward you to remove the base. Fig.

  • Page 90: Troubleshooting

    3.6 Troubleshooting Upon turning on the POWER switch of the spectrophotometer, the computer carries out a self-diagnosis automatically. If an abnormality is found in the instrument, an error message corresponding to the content of the abnormality will appear. Table 3-2 lists the error messages apt to appear. Be sure to observe the following caution when checking and/or remedying a trouble.
  • Page 91 (cont’d) Symptom Cause Judgment Method Remedy Optical signal not Error in parameter setting Check opening/ closing of Set the correct emitted shutter plus measurement parameters. parameters. Xe lamp not positioned Check with reference to Readjust the lamp correctly Fig. 3-4. position.

  • Page 92: Error Message List

    3.6.2 Error Message List Table 3-2 indicates the error information that may appear on the screen of the PC. If any of the errors shown here appear, take a remedy as indicated in the table. Table 3-2 Error Message List Error Message Probable Cause Remedy...

  • Page 93: Replacement Parts

    Several consumables are used in the spectrophotometer. To minimize its shutdown time, it is recommended to prepare an adequate number of those parts. The parts given below are consumables. Contact your nearest Hitachi High-Technologies Corporation sales representative or service office of Hitachi High-Technologies Corporation sales representative before consuming all of them.

  • Page 94: Limited-Life Parts

    It is recommended to replace the part with a new one before it reaches the end of its life with reference to the standard value for replacement. When ordering the part, contact your nearest Hitachi High-Tech Science Corporation sales representative or service office of Hitachi High-Technologies Corporation sales representative.

  • Page 95: Replacement Of Xenon Lamp

    4.3 Replacement of Xenon Lamp Replace the xenon lamp with a new one before its cumulative turn-on time exceeds the limit for use. When the cumulative turn-on time of the lamp reaches the useful limit, vapor and dispersed particles from the electrodes will adhere to the lamp wall and darken it, causing a decrease in heat dissipation.
  • Page 96 Observing the above cautious instructions, replace the xenon lamp in the following procedure. Disconnect the power cord from the connector block of the main unit. (Refer to Fig. 4-2.) The light source compartment cover cannot be removed with the cord connected to the connector block.
  • Page 97 Detach the light source compartment cover by pulling it up. Before proceeding to lamp replacement, wear proper protective gear such as safety mask, thick long sleeves and gloves. Remove the high voltage terminal screw at the anode cord side by turning it by hand. (Refer to ③ in Fig.
  • Page 98 Loosen the lamp holder by turning the lamp holder retaining screw counterclockwise by hand. (Refer to ⑤ in Fig. 4-5.) Fig. 4-5 Removal of Lamp Holder (2) While holding the lamp holder as shown in Fig. 4-5, pull it out by lifting up its screw part. In so doing, take care that the cathode side of the lamp does not hit against the base at the bottom.
  • Page 99 (10) Place the removed lamp holder on a flat and clean table, and proceed to detaching the xenon lamp. Fig. 4-7 Remove the nut from the cathode side of the xenon lamp while holding its metal part (refer to ⑦ in (11) Fig.
  • Page 100 Remove the nut from the anode side of the xenon lamp while holding its metal part (refer to ⑧ in (12) Fig. 4-9) by hand. Fig. 4-9 (13) Prepare a new xenon lamp. Before handling the xenon lamp, carefully read the instructions contained in its case. Do not touch the quartz glass part of the lamp with bare hands.
  • Page 101 Remove the nut from each electrode of the xenon lamp. (Refer to ⑨ in Fig. 4-10). (14) Fig. 4-10 Insert the anode-side electrode (marked with “+”) into the “+”-side electrode support metal of the (15) lamp holder. WARNING Be sure to mount the xenon lamp in the specified direction. If the mounting direction (polarity) is wrong, the cathode will be consumed significantly to disable turn-on of the lamp.
  • Page 102 (16) Tighten the nut while holding the metal part of the anode-side electrode of the xenon lamp (refer to ⑩ in Fig. 4-11) by hand. WARNING If the nut on the lamp base and wiring part is loose, the contact resistance between them will increase due to poor contacting.
  • Page 103 (17) Connect the cathode cord to the cathode-side electrode of the xenon lamp. In so doing, tighten the nut while holding the metal part of the cathode-side electrode (refer to ⑫ in Fig. 4-13) by hand without fail. Otherwise the xenon lamp could be adversely affected by intense mechanical strain imposed on its glass part.
  • Page 104 (18) Hold the retaining screw of the lamp holder tightly and set the holder into the lamp source housing slowly from above. In so doing, be careful not to hit the cathode side of the lamp shown in Fig. 4- 14 against the base at the bottom (refer to ⑬...
  • Page 105 Also set the lamp holder so that lamp holder retaining screw will not be skew. Fig. 4-16 (19) Fasten the anode cord to the HV-side terminal block and the cathode cord to the COM-side terminal block with the terminal screws. (Refer to ⑭ in Fig. 4-17.) Fig.
  • Page 106 (20) Put back the light source compartment cover in place, and fix it with the two retaining screws. (Refer to ⑮ in Fig. 4-18.) Fig. 4-18 (21) The replacement of Xe lamp has now been completed. Then carry out positional adjustment of the light source described in 3.1.

  • Page 107: Disposal Of Xenon Lamp

    4.4 Disposal of Xenon Lamp To request for disposal of the used xenon lamp, contact your nearest Hitachi High-Technologies Corporation sales representative or service office of Hitachi High-Technologies Corporation sales representative. When disposing of the lamp on your own, take the following procedure.

  • Page 108: Replacement Of Power Fuse

    If the power fuse is blown for any reason, then replace it with a new one in the procedure given below. If this trouble recurs, the instrument may be defective. Contact your nearest Hitachi High-Technologies Corporation sales representative or service office of Hitachi High-Technologies Corporation sales representative.
  • Page 109 The fuse holder is located above the power connector at the left side of the main unit. Press its knob at one side with a blade-edged screwdriver or the like. The holder will protrude somewhat. Then press the knob at the other side in the same way. The whole fuse holder will slightly protrude. You can now pull out the holder by hand.
  • Page 110 Fig. 4-22 4 - 18...

  • Page 111: Index

    INDEX A/D converter ............................. 2-9 Acquisition of noise level .......................... 3-15 Acquisition of signal intensity ........................3-15 Adjustment of light source position ......................1-30 Anode cord ..........................1-10, 3-8, 4-5 Antiseismic provision ..........................1-2 Auto pre-scan ............................3-26 Automatic Calibration and Self-diagnosis Functions ................2-11 Beam splitter ..............................
  • Page 112 D/A output port ............................2-6 Delivered Items ............................1-7 Diffraction grating ..........................2-10, 2-12 Drift ................................3-18 Emission side lens ..........................2-8, 3-20 Excitation side lens ........................... 2-8, 3-20 Emission ..............................3-2 Entrance slit ............................. 2-10 Error information ............................3-26 Exit slit ..............................
  • Page 113 Initialization .............................. 1-24 Installation ..........................1-1, 1-15, 1-29 Instrument configuration ..........................2-1 Lamp holder ..........................1-10, 3-6, 4-5 Lamp usage ............................. 3-19 Light source air-cooling louvers ......................... 2-3 Light source compartment cover......................2-3, 3-6 Limited-life parts ............................4-2 Long-life xenon lamp ..........................4-1 Maintenance and inspection ........................
  • Page 114 POWER switch....................1-7, 2-2, 2-4, 3-13, 3-25, 4-3 Photometric system ..........................2-12 Photomultiplier ............................2-9 Photomultiplier voltage ..........................3-4 Power connector ..........................1-7, 2-4 Power supply voltage ........................ 1-4, 1-7, 2-12 Pre-scan ..............................3-11 Quantitative calculation ..........................2-11 RUN indicator lamp ............................ 2-2 Raman spectrum ..........................
  • Page 115 Time lag fuse .............................. 4-1 Troubleshooting ............................3-24 USB cable ............................1-13, 2-5 USB communication ..........................2-12 USB driver ..............................1-19 Usage time of Xe lamp ..........................3-19 VERTICAL ............................2-3, 3-3 Wavelength accuracy ..........................2-12 Wavelength drive mechanism ........................2-11 Wavelength motor ............................

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