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Hitachi F-7100 Instruction Manual
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Hitachi F-7100 Instruction Manual

Hitachi F-7100 Instruction Manual

Fluorescence spectrophotometer
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INSTRUCTION MANUAL
5J1-9021-000 Ver.01 February. 2016
HITACHI F-7100
FLUORESCENCE
SPECTROPHOTOMETER
(SEARVICE MANUAL)

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  • Page 1 INSTRUCTION MANUAL 5J1-9021-000 Ver.01 February. 2016 HITACHI F-7100 FLUORESCENCE SPECTROPHOTOMETER (SEARVICE MANUAL)
  • Page 2 Hitachi High-Tech Science Corporation. 3. Hitachi High-Tech Science Corporation assumes no liability for any direct, indirect, or consequential damages arising from use not described in this manual.
  • Page 3 FOREWORD  This instruction manual describes the handling of F-7100 for its users.  The F-7100 fluorescence spectrophotometer is intended for fluorescence analysis, and should not be used for other purposes.  This instrument is aimed for personnel trained in basic chemical analysis.
  • Page 4 Note that samples that may have been infected with bacteria or viruses are not applicable to the instrument. The F-7100 is designed for use by personnel trained in and acquainted with chemical analyses. Be aware that appropriate handling of analytical equipment, reagents, or samples is essential for obtaining proper results and preventing safety-hazardous situations.
  • Page 5 IMPORTANT Precautions on CE Conformity Marking In consideration of use in the European countries, this instrument bears the CE mark indicating the conformity to the requirements mentioned below. (1) Electromagnetic Compatibility Requirement This instrument is designed to satisfy the European Norm EN61326-1 (2013) for the CE conformity marking through conformity to the EMC Directive 2004/108/EC.
  • Page 6 (2) In other Countries outside the EU If you wish to discard this product, please contact your local authorities and ask for the correct method of disposal. Instruction Manual for F-7100 Cautions The following is a statement of notice about EMC for Korea.
  • Page 7 Warranty on Product The Hitachi F-7100 Fluorescence Spectrophotometer is warranted to be free from defects in material or workmanship under normal use within the product specifications indicated in this manual and under conditions given below. This warranty is void if the instrument is not used in accordance with the instruction manual.
  • Page 8 (f) Failure due to improper handling or maintenance by the user. (g) Failure due to maintenance or repair by a service agent not approved or authorized by us. (h) Failure due to relocation or transport after the initial installation. Failure due to disassembly, modification or relocation not approved by Failure due to acts of God, including fire, earthquake, storm, flood, lightning, social disturbance, riot, crime, terrorism, war (declared or undeclared), radioactive pollution, contamination with harmful...
  • Page 9 (8 h/day, 20 /month)) For using the instrument beyond the useful service life, it shall be checked for safety by Hitachi High-Technologies Corporation sales representative or service office of Hitachi High-Technologies Corporation sales representative. (This safety check will be available on a chargeable basis.) If use of the instrument is continued without receiving the safety check, the instrument might become faulty and cause a danger.
  • Page 10 (c) The maintenance and consumables of the instrument can be supplied within the useful service life of the instrument (7 years). Even after the period of useful service life, the parts and units can be supplied (within 10 years after the date of initial use) so far as they are obtainable. However, this measure will not lead to an extension of the 7-year useful service life which is assured by the manufacturer.
  • Page 11 SAFETY SUMMARY Precautions on Safety Before using the F-7100 fluorescence spectrophotometer, be sure to read through and understand the safety instructions given below. The hazard warnings that appear on the warning labels on the product or in the manual have one of the following alert headings consisting of an alert symbol and signal word DANGER, WARNING or CAUTION.
  • Page 12  Do not perform any operation or action other than described in this manual. When in doubt, please contact your nearest Hitachi High-Technologies Corporation sales representative or service office of Hitachi High-Technologies Corporation sales representative.
  • Page 13 Hitachi High-Technologies Corporation sales representative or service office of Hitachi High-Technologies Corporation sales representative. SAFETY - 3...
  • Page 14 If any components are missing or damaged or if you have any question, consult the installation engineer, or the dealer from whom you purchased the instrument, or your nearest Hitachi High-Technologies Corporation sales representative or service office of Hitachi High-Technologies Corporation sales representative.
  • Page 15 For other than the replacement procedures instructed in this manual, contact your nearest Hitachi High-Technologies Corporation sales representative or service office of Hitachi High-Technologies Corporation sales representative.  For reducing a risk of trouble occurrence due to physical deterioration, it...
  • Page 16 SAFETY SUMMARY WARNING Indications Electric Shock upon Contact with Instrument Interior  An electric shock caused by contact with the power supply (30 kV) of the xenon lamp could result in fatal or serious injury. Be sure to turn off the power switch before removing the light source cover for replacing the lamp.
  • Page 17 SAFETY SUMMARY WARNING Indications (Continued) Injury due to Xenon Lamp Explosion [During use] When the cumulative turn-on time of the xenon lamp reaches the limit for use, vapor and dispersed particles from the electrodes will adhere to the lamp wall and darken it, causing a decrease in heat dissipation.
  • Page 18 SAFETY SUMMARY WARNING Indications (Continued) (5) Be sure to wear proper protective gear such as safety goggles, safety mask, thick long sleeves, and gloves when handling the xenon lamp. Do not touch the quartz glass part of the lamp with bare hands.
  • Page 19 SAFETY SUMMARY WARNING Indications (Continued) [At disposal] For disposal of the used xenon lamp, wrap it in a thick cloth (such as triple-folded cotton cloth) completely and crush its glass part with a hammer or the like. Then discard the lamp properly as a hazardous waste item. (Section 6) SAFETY - 9...
  • Page 20 SAFETY SUMMARY CAUTION Indications Touching Hot Part Could Result in Burns The xenon lamp and its housing become very hot during operation and remain hot for a while after operation. Touching the lamp or housing in this condition could result in severe burns. Before proceeding to lamp replacement, turn off the power switch and wait for at least one hour until the lamp and its housing cool down sufficiently.
  • Page 21 SAFETY SUMMARY CAUTION Indications (Continued) Prevent Tipover of Instrument Personal injury could be incurred if the instrument tips over. When installing the instrument, secure it properly to prevent tipover. (Section 5) SAFETY - 11...
  • Page 22 SAFETY SUMMARY Warning Labels The warning labels indicated below are attached to the F-7100 fluorescence spectrophotometer. SAFETY - 12...
  • Page 23 Contents PREFACE HOW TO USE THIS SERVICE MANUAL 1 Outline 1.1 Signal Process System and Control System 1.2 Optical System 1.3 Initialize Flow Chart 1.3.1 Photometer Initialize Flow Chart 1.3.2 Wavelength Initialize Flow Chart (EX、EM) 1.3.3 Slit Initialize Flow Chart （EX,EM） 1.3.4 Shutter Initialize Flow Chart Features...
  • Page 24 3.Explanation of circuit action 3.1 F-7100 circuit structure 3.2 Circuit function of FCPU circuit board that classified by module 3.3 Circuit function of FIO circuit board that classified by module 3.4 Main CPU Controls function 3.5 Sub CPU Controls function 4.
  • Page 25 6. Maintenance 6.1 Positional Adjustment of Light Source 6.1.1 Xe Lamp Visual Adjustment (coarse adjustment) 6.1.2 Adjustment of Xe Lamp Position Using Distilled Water (fine adjustment) 6.2 Check of Performance 6.2.1 Check of EM Wavelength Accuracy 6-12 6.2.2 Check of EX Wavelength Accuracy 6-14 6.2.3 Check of Sensitivity 6-17...
  • Page 26 7-15 7.3.1 FIO 7-16 7.3.2 OYA 15V into 100V DC-DC Convert module 7-16 7.3.3 OYB 24V into 15V DC-DC Convert module 7-17 7.3.4 OU8 24V into 5V DC-DC Convert module 7-17 7.3.5 OY7 Xe Lamp ON/OFF Control module 7-18 7.3.6 OY8 Wavelength Scan motor, Shutter motor Drv module...
  • Page 27 1 Outline Signal Process System and Control System Figure1-1 shows signal process system and control system of F-7100. The light emitted from the Xenon lamp enters the excitation monochromator, is divided by the beam splitter, and one beam enters the monitor detector.
  • Page 28 For example, in phosphorescence mode, the chopper turns to chop the excited light. (Excited light is made into a pulse.) Excitation concave Emission concave grating G1 grating G2 Chopper (Shutter) Monitor Sample cell Photomultiplier tube Xe lamp Fig.1－2 Optical System of Model F-7100 Fluorescence Spectrophotometer...
  • Page 29 1.3 Initialize Flow Chart 1.3.1 Photometer Initialize Flow Chart ＳＴＡＲＴ Ｎ ROM Check Error Ｙ Communications with Photometer impossible Ｎ RAM Check Error (One or more errors) Ｙ Wavelength Ｎ Error Initialize Performs for both EX, EM simultaneously Ｙ Slit Ｎ...
  • Page 30 1.3.2 Wavelength Initialize Flow Chart (EX、EM) ＳＴＡＲＴ 546nm position Ｎ Error detection Ｙ Set to 220nm(EX) Set to 500nm(EM) ＥＮＤ Fig.1-4 1.3.3 Slit Initialize Flow Chart (EX、EM) ＳＴＡＲＴ PI position Ｎ Error detection Ｙ Move to 1.0nm ＥＮＤ Fig.1-5 1.3.4 Shutter Initialize Flow Chart ＳＴＡＲＴ...
  • Page 31 Features 1.4.1 Comparison Table 1-1 Comparison Table Item F-7100 F-7000 F-2700 Stand-alone control Not available Not available Large LCD & Soft button PC control Standard Standard Available (optional software) Sensitivity 20000 RMS 15000 RMS 10000 RMS (BG method) Sensitivity 1200 RMS...
  • Page 32 Cut-off filters 295, 320, 370, 395, 295, 320, 370, 295, 320, 370, 395, 420nm 395, 420nm 420nm (optional filter set) (optional filter set) (optional filter set) Automatic Filter Automatic Filter option option PC connectivity Size:(W) x (D) X (H) cm 62 x 52 x 30 62 x 52 x 30 60 x 50.3 x 34.3...
  • Page 33 1.5 Motor Control 1.5.1 Slit 1. Composition and specification 24° 30° 18.4 Shading position and PI detection position 18.4 0.139mm/1nm Fig.1-7 Band pass EX： 1, 2.5, 5, 10, 20nm、and Zero（Shutter） EM： 1, 2.5, 5, 10, 20nm、and Zero（Shutter） Note： The EX and EM side has the reverse position of PI detection. 2.
  • Page 34 3c. Position adjustment for each slit The pulse that is 96pulse × moving destination slit numbers will output by CW/CCW. When moving in the CCW, the width of Slit spreads. ＜EX＞ When moving in the CCW, 24Pulse backlash correction is performed. ＜EM＞...
  • Page 35 1.5.2 Wavelength Drive 1. Composition and Specification PC2(High-speed scan detection） PC1(Low-speed scan detection） Grating WL motor Fig.1-8 Wavelength Drive（EX、EM） Cam： 0.2nm/nm、4,800Pulse (One rotation) Gear ratio： 1/6 0 ～200nm： 355Pulse 200～900nm： 3500Pulse 0 order light 546nm Fig.1-9 Wavelength Cam...
  • Page 36 2. Motor specification Model： PK264M-01B-X(Oriental Motor) X： Special specification for the drive wire. Rotation inertia moment： 120gfcm Rated voltage： 5.7V Rated current： 1A/phase Full Step： 0.9° Excitation maximum geostationary torque： 0.39N･m 3. Motor control specification Pulse output: CW / CCW two-way When wavelength is moving, pulse output is 5000/1000pps When measurement being done, pulse output is 1.25～5000pps 2500/5000pps: Slow up / Slow down...
  • Page 37 4. Slow up/Slow down It accelerates up to 5000pps(or 2500pps) with 20Pulse(4nm). Similarly, it decelerates with 20Pulse, and then it stops. 5,000 Pulse rate 20Pulse Pulse Fig.1-10 Table 1-3. Slow up table for 5000pps Start Pulse Rate End Pulse Rate Increase Pulse rate Pulse Number 1000...
  • Page 38 (ⅱ) When WL is less than 20nm in the CCW direction (short WL side), it shifts up to the position which is 4nm far away from target WL and stops with 1000Pulse. 20Pulse backlash correction will be performed at the stop position in CW direction with 500pps.
  • Page 39 WL shift in the range of 200nm is ordinary WL shift From 200nm it outputs 355+20Pulse in the CCW direction (short WL side) with 200pps. From the stop position, 20pulse backlash correction is done in the CW direction with 200pps. 4b.
  • Page 40 4c. 3-D Scan (ⅰ) When scanning speed is 12000nm/min or less, WL will shift to the EM start WL position after EM Scan has finished (Backlash correction is performed) It shifts to the next EX WL and EM WL scans. Wavelength EM WL Start WL...
  • Page 41 4d. 3-D Time Scan 3-D Time Scan is a repetition of WL Scanning. When scan speed is 60,000 or 30,000 nm/min, backlash correction is not performed. (refer to ①＆② of 3-D scan). After the last measurement is finished, WL will move back to the start WL. (Backlash correction) 4e.
  • Page 42 1.5.3 Chopper 1. Composition and specification The PI detection position is Beam penetration center position. Beam street position Light Beam 10.5° Relation between Beam and Apache Motor Pulse （ Phosphorescence 0.5ms 0.5ms 0.5ms Phosphorescence Quantities of light change 0.4ms 1.5ms 0.4ms 0.75ms Transmit...
  • Page 43 2. Motor specification Model：PK244-01B-X(Oriental Motor) Rated voltage：4V Rated current：1.2A/Phase Full Step：0.9°/Pulse (400Pulse/ One rotation) Excitation maximum geostationary torque：0.26N･m Rotation inertia moment：54gfcm Motor Control specification The shutter automatically operates when pulse is 400pps. The chopper is slow up when pulse is 1,000pps、2,000pps、4,000pps.（and chopper） Table 1-5.
  • Page 44 6. Zero Adjustment、and shutter close 50Pulse is output in the direction of CCW with 400pps and it stops. （It inclines by 45 degrees and it stops.） After Zero Adjustment ends, it moves to the initialization operation doing and the beam penetration position.
  • Page 45 1.6 USB Communication control 1.6.1 Composition USB1.1 Full speed (12MBPS) Fig 1-20 USB Communication control part composition 1.7 ACC port Specification 1.7.1 Composition of ACC port Fig 1-21 Accessories Connected port 1-19...
  • Page 46 1.7.2 Accessories Communication port1 20bit DAC F-7000 サイドパネル F-7000/7100 Side panel +5V(0.3A) ダウンロード Dawn load Mode モード指定信号 Set signal (オープン) (open) Fig 1-22 1.7.3 Main CPU program download function of Accessories Communication port1 It doesn't respond excluding download of Program. At download of Main CPU Program Dawn load Mode Set signal (open)
  • Page 47 1.8 Armature voltage control of Photomultiplier 1.8.1 Composition Output voltage is monitored by ADC built into SH2, and the PWM control data is proofread. Fig.1-24 Lighting Check Xe lamp and control of turning off 1.9.1 Composition Fig.1-25 1-21...
  • Page 48 2 Specifications 2.1 General Specifications Table 2-1 General Specifications Large size aberration corrected concave grating; 900line/mm Monochromator Eagle mount：F2.2 Blaze Wavelength：300mm(EX)、400mm(EM) Wavelength range Both Excitation, Emission 200-750nm, 0-order light (EX、EM) And enable to expand 900nm for option detector. Resolution 1.0nm±25% (435.8,546.1nm) Wavelength is accuracy Within±1.0nm (at 435.8nm) 30, 60, 240, 1200, 2400, 12000, 30000, 60000nm/min...
  • Page 49 3. Explanation of circuit action 3.1 F-7100 circuit structure F-7100 Power supply Xe lamp power supply XB4-150HX2 24V power supply JWS-100 Input fuse Line filter P･C RS232C Door SW Chopper Sub CPU program motor wavele wavele (Appropriation cable) ngth ngth...
  • Page 50 3.2 Circuit function of FCPU circuit board that classified by module Fig.3-2 Circuit function of FIO circuit board that classified by module Fig.3-3...
  • Page 51 3.4 Main CPU Controls function （１） Communication control PC to USB Main CPU receives measurement command from PC (USB Ver.1.1), and send measurement data. （２） ACC control ACC port１：motor control ACC ACC port２：Intelligent ACC ACC communication port １：20bit DA converter （３）...
  • Page 52 Sub CPU Controls function （１） EX・EM wavelength and shutter motor controls Invented motor control IC for wavelength scan speed to high, therefore it is in controls high speed of motor control from SUB CPU command communication. Motor control IC：MC68HC908QY 0Y5IC1～IC3 （２）...
  • Page 53 4 Troubleshooting Troubleshooting Table Table 4-1 Troubleshooting table Symptom Possible Cause Checking way Repair Power fuse blown Replace fuse with a new Check fuse Light Source cover is Light Source cover is open. Check Light Source cover close. When MAIN switch is turned Wait until lamp Lamp is not cooled on, discharge flashes, but...
  • Page 54 4.2 Error Information Table 4-2 shows Error Information presented while the screen is displayed. If such an error appears, follow ‘Repair “ in the Table. Error Information Table 4-2 Message Presented Possible Cause Repair Error is ROM Replace FCPU board with a ROM Status Error! new one.
  • Page 55 5. Part replacement Composition of Main body Fig . 5-1 shows the composition of F-7100 main unit. ① ⑲ ⑱ ⑰ ⑯ ⑮ ② ⑭ ③ ④ ⑤ ⑥ ⑦ Fig . 5-1 ⑧ ⑨ ⑩ ⑪ ⑫ ⑬ ① Xe lamp power supply ⑪...
  • Page 56 5.3 Disassembly 5.3.1 Removing main body cover WARNING Contact with the power supply voltage (100V) may cause an electric shock, resulting in fatal or serious injury. Before removing the main cover, make sure that the POWER switch of the spectrophotometer main unit is turned off. WARNING Contact with Xenon lamp (30kV) may cause an electric shock, resulting in fatal or serious injury.
  • Page 57 This procedure is same as F-7000. (1) Remove the light source cover of the left side of the main unit Remove fixing screws of the cover Fig . 5-2 Power cover (2) Firstly, remove the sample compartment referring to Fig . 5-3. (Remove the screws with ○...
  • Page 58 5.3.2 Replacement of Photomultiplier (1)Remove six screws with ○ marks of Fig .5-7, then gently remove the monochromator cover. Fig . 5-7 The main cover was removed (1) Remove two fixing screws of the photo-multiplier cover, then remove the cover show in Fig .5-8.
  • Page 59 5.3.4 Replacement of FCPU circuit board Important Precautions on FCPU circuit board Replacement When FCPU circuit board is replaced, be sure to install the same serial number into the new board. Before connecting with PC, be sure to install the serial number into FCPU circuit board. (Please refer to 6.5 of Chapter 6 for the details.) (1)Remove one fixing screw of the connector cover on the light side of main unit with ○...
  • Page 60 5.3.5 The Replacement of Photo-interrupter The following two ways of operation on the replacement of Photo-interrupter are available. One is that removed photo-interrupter directly from the mounting plate, the other is that removed photo-interrupter after the adjusting bolt and the mounting plate being removed. (1) How to remove photo-interrupter directly from the mounting plate Referring to Fig.5-15a and Fig.5-15b, remove photo-interrupter.
  • Page 61 How to remove the mounting plate Referring Fig.5-15c and Fig.5-15d, remove photo-interrupter after removing two adjusting bolts and the mounting plate. Two adjusting bolts Mounting plate Photo-interrupter Fig.5-15c mounting plate（WL Cam） Mounting plate Two adjusting bolts Photo-interrupter Fig.5-15d mounting plate（WL Motor） Note：...
  • Page 62 5.3.6 Removing light source unit (Lamp house) Important Precautions on removal of light source unit The position of the light source unit is optically adjusted and fixed. Before removing it, please note that a ruled line for the location of the light source unit should be drawn on the base.
  • Page 63 (2) Loose two screws with ○ marks as shown in Fig .5-18 and remove the wavelength drive unit. WL drive unit Fig .5-18 Wavelength drive unit (2） 5.3.8 Removing monitor detector (1) Loose two screws with ○ marks as shown in Fig .5-19 and remove monitor detector with mounting plate.
  • Page 64 5.3.9 Removing Slit unit 〈Excitation side〉 (1) Referring to 5.3.8 remove monitor detector. (2) Remove four screws with ○ marks as shown in Fig. 5-20, then remove EX mirror unit. EX mirror unit Fig .5-20 excitation slit(1） (3) Remove two screws with ○ marks as shown in Fig . 5-21 and remove slit unit. Slit unit Fig .5-21 excitation slit(2）...
  • Page 65 5.3.10 Removing chopper (1) Remove two screws with ○ marks as shown in Fig .5-22 and remove chopper. Chopper Fig .5-22 Chopper 5-11...
  • Page 66 5.4 Optical axis check The image of beam in sample compartment and monochromator is shown below. 5.4.1 EX slit These images of incident and exit beams are shown Fig .5-23. (Check these images after remove the chopper and EX mirror.) Beam EX incident slit Beam...
  • Page 67 5.4.4 EM slit Check fluorescence beam of Rhodamine B solution on the slit. Using 10mm cell and 0 order light to confirm it. Beam EX exit slit Fig .5-26 EM slit image 5.4.5 Check points of Optical axis EX Slit (5.4.1) Grating (5.4.2) Sample compartment(5.4.3) EX lens EM slit (5.4.4)
  • Page 68 6.Maintenance The fluorescence spectrophotometer requires maintenance in order to keep it in proper running condition. This chapter explains the procedures for positional adjustment of the light source, check of performance, maintenance & inspection, and troubleshooting. 6.1 Positional Adjustment of Light Source Carry out the following adjustment when the Xe lamp has been replaced, when the lamp holder has been detached, or when the lamp positioning screw has been manipulated.
  • Page 69 A window as in Fig. 6-1 will open. Set the EX wavelength at 546 nm. Fig. 6-1 Place a sheet of white paper in front of the EX light exit lens, and adjust while watching the light beam shape. Excitation light (Excitation) exit window White...
  • Page 70 Using the supplied stubby screwdriver, turn any one of VERTICAL ADJ., HORIZONTAL ADJ. and FOCUS on the left side of the main unit and a green light will appear. Adjust so that the light beam shape on the excitation light exit window will resemble the shape of the EX lens light beam shown in Fig.
  • Page 71 Adjust with HORIZONTAL ADJ. so the light at the cell inlet will come to the center of the cell holder window. Cell inlet Cell holder Light beam Cell holder window Fig. 6-5 This completes the coarse adjustment of the Xe lamp position. Now proceed to fine adjustment of the lamp position.
  • Page 72 Fill the supplied quartz fluorescence cell with distilled water and set it in the cell holder in the sample compartment. After the above setting, click OK button. Click (start measurement) button on the measurement toolbar. The time scan measurement will start. Now adjust VERTICAL ADJ. and FOCUS on the left side of the main unit, using the supplied stubby screwdriver, so the values on the graph will become maximum.
  • Page 73 6.2 Check of Performance 6.2.1 Check of EM Wavelength Accuracy Utilizing the Hg bright lines included in a fluorescent lamp located in the room where the instrument is installed, measure the spectrum in the vicinity of the bright lines and check the difference between the wavelength of bright lines and the wavelength indication.
  • Page 74 Unplug the power cord from the main unit connector (see Fig. 6-6). The light source compartment cover cannot be removed with the cord inserted in the connector. Fig. 6-6 Detachment of Power Cord Remove the two screws holding the light source compartment cover on the left side of the main unit (①, ②...
  • Page 75 Be sure to put on a safety mask, thick long sleeves, gloves, etc. prior to the removal procedure. Turn by hand and remove the high voltage terminal terminal screw on the anode cord side (③ in Fig. 6-8). An insulating stopper is attached to this terminal screw so it will not drop off and be lost. Turn by hand and remove the high voltage terminal terminal screw on the cathode cord side (④...
  • Page 76 Hold the lamp holder as shown in Fig. 6-10, and while grasping the screw part of the holder, lift the whole unit a little upward and detach it. Be careful when removing so the cathode side of the lamp will not hit the lower base. Fig.
  • Page 77 (12) Insert the power cord into the connector on the main unit. Then start up the spectrophotometer with reference to startup of the FL Solutions 2.1 program. (13) Set the supplied white paper for WL check at a slant in the cell holder of the sample compartment (Fig.
  • Page 78 (15) Click the (Pre Scan) button on the measurement toolbar. A pre-scan will thus be executed. (16) Click the (start measurement) button on the measurement toolbar. (17) When a spectrum is acquired, carry out peak detection and determine the peak wavelength. Make sure it is within the specification.
  • Page 79 6 .2.2 Check of EX Wavelength Accuracy Take the following procedure for the check. Click the (analytical method) button on the measurement toolbar and set “wavelength scan” for measuring mode under the [General] tab on the Method window. Then select the [Instrument] tab and set the conditions given below.
  • Page 80 Close the FL Solutions 2.1 program. Turn OFF the POWER switch of the spectrophotometer main unit. Unplug the power cord from the main unit connector. Detach the cover from the light source compartment and close the lid over the hole at the reverse of the light source compartment cover.
  • Page 81 6.2.3 Check of Sensitivity Check the signal-to-noise ratio on a Raman spectrum of water (Raman scattering) under fixed measurement conditions. For this sensitivity check, be sure to use a clean fluorescence cell containing fresh distilled water. The procedure is as follows. To ensure stability of the Xe lamp, wait at least 30 minutes after turning on the lamp.
  • Page 82 [Reference] Auto parameter setting (fixed)  <Sensitivity> Wavelength scan (acquisition of signal intensity)  EX and EM Slits : Both 5 nm  EX WL 350 nm  EM WL Raman peak wavelength  Response  Scan speed 60 nm/min ...
  • Page 83 Time Scan  EX and EM Slits : Both 10 nm  EX WL 350 nm  EM WL 420 nm  Response  Scan Time 601 s  PMT Voltage 700 V Calculation method   Calculation of the noise level (N) with the peak to peak definition Determine the peak position and photometric value (“S”...
  • Page 84 <Calculation method>  Height of Raman peak (S)  Noise level (N) 10 s 10 s 10 s N = average value of N … Peak wavelength Baseline Fig. 6-14 Check of Sensitivity Specifications: S/N 20000 or more (RMS) [BG] S/N 1200 or more (RMS) S/N 360 or more (Peak to Peak) 6 - 17...
  • Page 85 6.2.4 Stability Determine the variation with time of emission intensity (including scattered light) under fixed measurement conditions. Conduct the measurement simultaneously with that in 6.2.3. The results will be indicated as in Fig. 3-13. Auto parameter setting (fixed)  Time scan mode ...
  • Page 86 6.3 Calibration The calibration data is created and saved as a file not into the main unit but PC for F-7100 fluorescence spectrophotometer. The following files that were created and saved are available. 1） WL Calibration data, Setup Gate Delay Time 2）...
  • Page 87 6.3.1 Input of WL Calibration data If the used PC wasn’t directly delivered from Hitachi factory, be sure to set up WL Calibration data according to the following procedures. （1） Install FL Solutions. （2） After turning on the main power switch of F-7100, FL Solutions is started.
  • Page 88 （6） Upon clicking the OK button the following input window of WL Calibration data will be displayed. Fig .6-19 (6) Input the calibration data (EX wavelength calibration data and EM wavelength calibration data ) and click the OK button, the calibration data can be saved in the Serial No,env. file of calibration folder.
  • Page 89 6.4 Service engineers mode（Function only for Service engineers） Service engineer mode is installed in FL solutions 2.1 for F-7100. Because this mode is function only for service engineers, and not being opened to customers, care should be taken when handling it.
  • Page 90 (2) To delete the past wavelength calibration data, please delete Serial No. env file in the calibration folder. Delete Fig .6-21 Then, the function of Service engineer mode becomes executable. Please inform from pull-down menu of utility menu. When executing, please input the password "123456". When this function come to end, please delete the Service.ini file in the service folder so that the customer cannot use it.
  • Page 91 The following operations that EX Auto WL Calibration and EM Auto WL Calibration being done by Service engineer mode are available. 《Preparation》 ・ Hg Pen lei lamp that service engineers prepared. ・ diffuser （P/N5J1-1990 ）that is the standard attached to F-7100. 1. Wavelength Calibration Procedure （1） Remove the light source cover.
  • Page 92 (7) After the following window of wavelength calibration that EX and EM are both 0nm is displayed, please click the OK button. Fig .6-24 (8) Select 「EX Auto WL Calibration」 from 「Utility」.(Input the password and execute) Fig .6-25 (9) Upon completing the operation, the calibration data indicates. Please fill the data into wavelength calibration data label.
  • Page 93 〈reference: Method of calculating wavelength calibration data〉 When Auto WL Calibration is done, the correction data is calculated and set as follows. ① Measure each wavelength of 253.7, 435.8, 546.1nm. (Each WL range is 240～270、420～450、530～560nm) ② Calculate the difference in the three points. ③...
  • Page 94 6.4.3 Gate Delay Time Gate Delay Time in each Chopping Speed is changed through his setting. But, if this value is not correctly set, the phosphorescence spectrum becomes abnormal. Usually it is not necessary to change, please keep up it as the default value (1) Select 「Gate Delay Time」...
  • Page 95 6.5 Setting Serial Number（Setup of FCPU Board） The manufacture number (serial number) of F-7000 is preliminarily setting as a code number of the FCPU Board in the spectrophotometer. This manufacture number (serial number) is setup with Rotary Switch of the FCPU board. This number is read out on the PC side when initializing it, and each calibration data of the same serial number in which the file is preserved in PC is transmitted to the spectrophotometer.
  • Page 96 Program Download Firmware is F-7000 and F-7100 commonness. F-7000/7100 is controlled ｂｙ FL Solutions program at the PC and Control program at the F-7000/7100 main unit. When control program at the main unit is updated, it is necessary to update the content of Main CPU (SH2) and Sub CPU (SH2) in FCPU board and download by ＰＣ...
  • Page 97 図 7-1 Fig.6-31 (2) When the following screen (Fig.6-32) displays, please select “Main CPU” and “COM Port” that will be used, and click the NEXT button. Fig.6-32 (3)When the following screen (Fig.6-31) displays, connect the cable（P/N5J1-2350）according to the screen guidance. Fig.6-33 (4)Turn off the F-7000/7100 main power switch.
  • Page 98 （5）Remove the connector cover fixing screw on right side of the spectrophotometer. Connector cover Screw Fig.6-34 (6)Use download cable for main CPU (P/N5J1-2350) to connect PC with F-7000/7100 (D/A connector). RS-232C D/A connector Fig.6-35 (7)Turn on the F-7000/7100 main power switch. (8)The download is begun as soon as the EXECUTE button (Fig .6-36) is clicked.
  • Page 99 (9) When download is completed normally, the following window (Fig . 6-37) appears. Turn OFF the F-7000/7100 main power switch, and remove the download cable. The main CPU download has finished. Fig.6-37.Download End 6.6.2 Download Procedure for the Sub CPU Firmware It continues to the above-mentioned procedure, should be download Sub CPU.
  • Page 100 (3)Turn OFF the F-7000/7100 main power switch. (4)Remove two CPU Board fixing screws, and pull out the CPU board. Fig.6-40 Screw CPU Board Fig.6-41 (5) Connect PC with ZJ18 connector by the download cable of Sub CPU (P/N5J1-2351). RS-232C Fig.6-42 ZJ18 Connector (6) Turn ON the F-7000/7100 main power switch.
  • Page 101 7 Ｃｉｒｃｕｉｔ Ｄｉａｇｒａｍ Total Wiring Diagram FCPU Circuit Diagram 7.2.1 FCPU（1/2）module 7.2.2 FCPU (2/2) module 7.2.3 OA1 Main CPU module 7.2.4 OA2 Sub CPU module 7.2.5 OUE RS232Cdrv (acc DAC, Main CPU d1) module 7.2.6 OY1 AMP ADC module 7.2.7 OY2 Communication between CPU module 7.2.8 OY3 Slit motor control module...
  • Page 103 7.2 FCPU Circuit Diagram 7.2.1 FCPU (1/2) module FCPU (1/2) module...
  • Page 105 7.2.2 FCPU (2/2) module FCPU (2/2) module...
  • Page 106 7.2.3 OA1 Main CPU module OA1 Main CPU module...
  • Page 107 7.2.4 OA2 Sub CPU module OA2 Sub CPU module...
  • Page 108 7.2.5 OUE RS232Cdrv (acc DAC, Main CPU d1) module OUE RS232Cdrv (acc DAC, Main CPU d1) module...
  • Page 110 7.2.6 OY1 AMP ADC module OY1 AMP ADC module...
  • Page 111 7.2.7 OY2 Communication Between CPU module OY2 Communication Between CPU module...
  • Page 112 7.2.8 OY3 Slit motor control module 7.2.9 OY4 Acc motor control module OY3 Slit motor control module OY4 Acc motor control module...
  • Page 113 7.2.10 OY5 Wavelength scan motor, shutter motor control module OY5 Wavelength scan motor, shutter motor control module 7-10...
  • Page 114 7.2.11 OY6 Production number setting module OY6 Production number setting module 7-11...
  • Page 115 7.2.12 OY9 IO Interface module OY9 IO Interface module 7-12...
  • Page 116 7.2.13 1UE RS232Cdr (ACCPORT2) module 1UE RS232Cdr (ACCPORT2) module 7-13...
  • Page 117 7.2.14 2UE RS232Cdrv (Sub CPU) module 2UE RS232Cdrv (Sub CPU) module 7-14...
  • Page 118 7.3 FIO Circuit Diagram 7.3.1 FIO 7-15...
  • Page 119 7.3.2 OYA 15V into 100V DC-DC Convert module 7.3.3 OYB 24V into 15V DC-DC Convert module OYA 15V into 100V DC-DC Convert module OYB 24V into 15V DC-DC Convert module 7-16...
  • Page 120 7.3.4 OU8 24V into 5V DC-DC Convert module 7.3.5 OY7 Xe Lamp ON/OFF Control module OU8 24V into 5V DC-DC Convert module OY7 Xe Lamp ON/OFF Control module 7-17...
  • Page 121 7.3.6 OY8 Wavelength Scan motor , Shutter motor Drv module OY8 Wavelength Scan motor, Shutter motor Drv module 7-18...