Plt And Ret: Sf Cube Cell Analysis Technology; Derivation Of Rbc - Related Parameters - scil Element HT5+ User Manual

Each pulse is amplified and compared to the internal reference voltage channel, which only
accepts the pulses of certain amplitude. If the pulse generated is above the RBC/PLT lower
threshold, it is counted as a RBC/PLT.
Compared with the common impedance method, the sheath flow impedance method is
featured by higher efficiency, better signal quality, more accurate analysis results and lower
consumption of reagents.

3.4.2 PLT and RET: SF Cube Cell Analysis technology

The RET channel also adopts the SF Cube Cell Analysis technology. The general
measurement principle in the RET channel is similar to that of WBC channel, only that in the
RET channel, the RBC are not lysed, but are spherized by RET diluent. Then the nucleic acid
of the spherized RBC, the PLT and the RET are stained by fluorescent dyes.
Therefore the analyzer provides a PLT count by combining the large-size platelet count result
in SF Cube channel and platelet count result in impedance channel.

3.4.3 Derivation of RBC - related parameters

MCV:
Based on the RBC histogram, the analyzer calculates the mean cell volume and expresses
the result in fL.
HCT, MCH and MCHC:
The analyzer calculates the HCT (%), MCH (pg) and MCHC (g/L) as follows:
������ × ������
������ =
10
������
������ =
������
Vers.: 20230710ENG
Page 22