Nikon DIAPHOT 300 Instructions Manual page 13

Inverted microscope
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10. Place a specimen on the stage.
If the specimen is on a slide glass, be
sure to mount it with its cover glass
facing down.
(See page 27.) Move
the stage to position the desired section
of the specimen into the viewfield.
Figure 2-10
1 1. Focus.
Rotate the refocusing stopper on the
left-hand focusing knob counter¬
clockwise to unlock the stopper.
Rotate the coarse/fine focusing knob to
focus on the specimen.
NOTE: Never turn the left-hand and
right-hand knobs in the
opposite directions at the same
time, as damage may result.
12. Center the condenser.
Narrow down the field aperture
diaphragm until the diaphragm edge is
viewed through eyepieces.
Rotate the condenser focus knob to
focus the image of the field aperture
diaphragm on the specimen surface.
Adjust the condenser centering screws
so that the center of the field aperture
diaphragm image matches the center of
the viewfield.
NOTE: If the SLWD condenser is used,
this centering is unnecessary.
(Fully open the field aperture
diaphragm.)
Figure 2-12
Figure 2-1 1
7

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