Nano Temper Prometheus Panta User Manual page 18

Figure 3: Capillary tray layout and loading for the Prometheus Panta instrument. The removable capillary frame can hold up to
48 capillaries, which are numbered from 1 to 48, starting in the front closest to the user. When filling the capillaries, it is
important that the capillaries are filled completely, and that they are centered on the capillary tray. Once all capillaries are
loaded onto the capillary tray, place the magnetic lid to fix the capillaries.
Alternatively, samples can be loaded into capillary chips. The capillaries on Prometheus nanoDSF Grade
24-Capillary Chips are spaced to enable easy and fast sample loading from 384-well microtiter plates. The
Capillary Chip Filling Station (Cat# NT-AT100) is recommended for manual loading of capillary chips from
microtiter plates since it places the sample at the optimal angle for loading (see Figure 4 B). Correct
positioning of the capillaries on the tray is ensured by the capillary chip format.
Place the chip onto the thermal element with the capillaries facing upward (see Figure 4 A). Use the
number  imprinted on the chip for orientation, for example to load your sample number 1. Use the
symbols next to the thermal element for correct chip placement. It is recommended to place the capillary
chip with the imprinted number  toward the user, since the capillary closest to the user will be
designated "capillary 1" by the software.
Next, place the lid slowly and evenly on top of the capillary chip (see Figure 4 A). The weight of the lid
holds the capillaries in place. Slight movements of the lid (and the capillary chip) inside of the guiding
structures are normal and will not affect the measurement.
CAUTION: Careless, hasty or diagonal placement of the lid can lead to damage of capillaries or the
thermal element. Keep hands away from the instrument when closing the sample drawer. Especially, be
careful not to get caught between capillary lid and instrument, since this can lead to injury and
instrument damage. If necessary, push the 'Cancel' button on the touch display of the instrument to stop
the drawer from closing.
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