Nano Temper Prometheus Series User Manual
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Nano Temper Prometheus Series User Manual

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Prometheus Series Instruments

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Summary of Contents for Nano Temper Prometheus Series

  • Page 1 User Manual Prometheus Series Instruments...

  • Page 2: Table Of Contents

    1.6. Sample Consumption .....................11 1.7. Capillary Format .....................12 1.8. Dedicated Control and Analysis Software ...............12 nanoDSF Technology ....................13 Using the Prometheus Series Instruments..............14 3.1. General Usage .......................14 3.2. Sample Loading .....................14 3.2.1. Sample Loading for the Prometheus NT.48 ............15 3.2.2.

  • Page 3: Safety Considerations

    Safety Considerations To ensure operation safety, this instrument must be operated correctly. Carefully read the following explanations to fully understand all safety precautions in this manual before operating the instrument. Please take a moment to understand what the signal words WARNING!, CAUTION and NOTE mean in this manual.
  • Page 4 Only use the provided cables and plugs. Failure to comply may result in a risk of electric shock and fire. CAUTION The Prometheus Series instrument has to be installed in a way that does not hinder access to the external power supply and its power plug.

  • Page 5: Regulatory Statement

    CAUTION Use the instrument only at noncondensing conditions (0–80 % humidity, 15-30 °C). At very high humidity levels, even normal operating temperatures may result in condensation and corrosion. CAUTION Turn off the instrument when not in use. CAUTION Do not use ethanol or other types of organic solvents to clean the instrument as they may remove the instrument paint.

  • Page 6: Technical Specifications

    Input of external power supply: 90–264 VAC ± 10 % 47–63 Hz, 230 VA max Output of external power supply: 24 VDC, 10 A max Electrical input to Prometheus Series instrument: 24 VDC, 10 A Environmental: Operating temperature 15 – 30 °C (indoor use only) Humidity 0–80 %, noncondensing...

  • Page 7: Connections

    Connections All ingoing and outgoing connections can be found on the rear panel of the instrument. Name Function Ethernet Socket for connecting to the PC/laptop via an Ethernet cable. Turning the switch to position “I” switches on the instrument. On/Off Button DC Input Connector to the external power supply.

  • Page 8: Preface

    Preface This manual is a guide for using Prometheus Series instruments and performing nanoDSF measurements. It instructs first-time users on how to use the instrument, and serves as a reference for experienced users. Before using the Prometheus Series instrument, please read this instruction manual carefully, and make sure that the contents are fully understood.

  • Page 9: Limited Warranty

    Limited Warranty Products sold by NanoTemper Technologies, unless otherwise specified, are warrantied to be free of defects in materials and workmanship for a period of one year from the date of shipment. If any defects in the product are found during this warranty period, NanoTemper Technologies will repair or replace the defective part(s) or product free of charge.

  • Page 10: Prometheus Series Instruments

    The Prometheus Series comprises two instruments. The Prometheus NT.48 is filled with up to 48 single capillaries for most flexible assay design and easy handling. The Prometheus NT.Plex employs 24-Capillary chips, which enables manual or automated sample loading. It can be coupled with robotic and liquid handling platforms for unattended operation and complete automation.

  • Page 11: Application Range

    1.2. Application Range The Prometheus instruments use nanoDSF technology to detect changes in the fluorescence of the amino acids tryptophan and tyrosine over a wide range of temperatures (see section 1.4). The instruments are used to induce thermal unfolding of proteins and to determine thermal unfolding transition temperatures.

  • Page 12: Capillary Format

    1.7. Capillary Format The capillary format of the Prometheus instruments is cost-effective, easy to handle and offers maximal flexibility in the experiment scale, while offering a detection precision that is superior to other approaches. The number of samples measured in one run can be any number from one to 48 capillaries in a Prometheus NT.48 instrument, or one to 24 capillaries in a Prometheus NT.Plex instrument.

  • Page 13: Nanodsf Technology

    While thermal unfolding experiments use a temperature ramp to monitor protein conformational changes, chemical unfolding experiments use chaotropes such as urea to unfold proteins. Both types of experiments are easily done with the Prometheus Series instruments. Figure 2: Schematic representation of protein unfolding.

  • Page 14: Using The Prometheus Series Instruments

    Figure 3 (top right panel). Once capillaries are filled, place them onto the capillary tray and fix the capillaries by placing the lid onto the tray. Differences in sample loading between different Prometheus Series instruments are described in the following sections. Note: NanoTemper Technologies offers two different types of capillaries, nanoDSF Grade Standard Capillaries and nanoDSF Grade High Sensitivity Capillaries.

  • Page 15: Sample Loading For The Prometheus Nt.48

    3.2.1. Sample Loading for the Prometheus NT.48 The Prometheus NT.48 capillary tray provides 48 numbered slots for single capillaries, offering maximum flexibility in assay design. It is important that the capillaries are positioned in the center of the tray. It is also important that the meniscus of the sample does not approach the heated capillary tray surface, since this will lead to rapid evaporation (Figure 3).

  • Page 16: Automated Sample Loading For The Prometheus Nt.plex

    Figure 4 B). Correct positioning of the capillaries on the tray is ensured by the capillary chip format. Place the chip onto the thermal element with the capillaries facing upward (see Figure 4 A).  Use the number imprinted on the chip for orientation, for example to load your sample number 1.
  • Page 17 Contact NanoTemper Technologies GmbH Floessergasse 4 81369 Munich Germany Phone: +49 (0)89 4522895 0 Fax: +49 (0)89 4522895 60 info@nanotemper-technologies.com http://www.nanotemper-technologies.com NanoTemper®, Prometheus and nanoDSF registered trademarks. V13_2017-0339-133322...

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