Prometheus Panta Instrument; Temperature Range; Capillary Format - Nano Temper Prometheus Panta User Manual

5. Prometheus Panta instrument

The Prometheus Panta instrument uses nanoDSF technology to detect changes in the fluorescence of the
amino acids tryptophan and tyrosine over a wide range of temperatures. The instrument is used to
induce thermal unfolding of proteins and to determine thermal unfolding transition temperatures.
Furthermore, thermal protein refolding can be monitored and refolding transition temperatures can be
determined.
Secondly, the Prometheus Panta instrument is equipped with backreflection optics to investigate
aggregation and precipitation in a sample upon heating and to determine aggregation onset
temperatures.
Thirdly, the Prometheus Panta instrument is equipped with DLS (dynamic light scattering) optics to
investigate the particle size and particle size distributions at constant temperature. Furthermore, particle
sizes and size distributions can be measured during thermal unfolding in parallel to nanoDSF and
backreflection to determine onset temperatures of size changes.

Temperature Range

The thermal element inside Prometheus instruments can be precisely adjusted from 15 °C to 95 °C.
Additionally, the instruments can be equipped with a High Temperature Upgrade allowing a maximum
temperature of 110 °C.
Note: If experiments exceed a temperature of 95 °C, or measurement times of 3 hours, capillaries must be
sealed using NanoTemper Technologies Capillary Sealing Paste (PR-P001) and Capillary Sealing Applicators
(PR-P002).
Note: For instruments equipped with a High Temperature Upgrade: When running experiments with a
maximum temperature of 110 °C, the minimum ambient temperature is 20 °C to ensure the instrument
reaches its target temperature.
Note: For measurement temperatures below 20°C, the ambient temperature should be
instrument reaches target temperature.

Capillary Format

The capillary format of the Prometheus Panta is cost-effective, easy to handle and offers maximal
flexibility in the experiment scale, while offering a detection precision that is superior to other
approaches. The number of samples measured in one run can be any number from one to 48 capillaries
or one to 24 capillaries when using capillary chips. Prometheus systems should only be used in
conjunction with high quality glass capillaries or capillary chips provided by NanoTemper Technologies.
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25°C to ensure the