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Related Manuals for Nano Temper Prometheus Panta
Summary of Contents for Nano Temper Prometheus Panta
- Page 1 Prometheus Panta User manual...
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Page 3: Table Of Contents
Prometheus Panta instrument ......................16 Temperature Range ........................16 Capillary Format ......................... 16 Using Prometheus Panta ........................17 General Usage ..........................17 Sample Loading .......................... 17 6.2.1. Sample Loading for the Prometheus Panta ................17 Patents and intellectual property ...................... 20 3/22... - Page 4 Transport and disposal ........................21 Repackaging for transport ......................21 Waste disposal ..........................21 System disposal .......................... 21 4/22...
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Page 5: About This User Manual
1. About this user manual This manual is a guide for using Prometheus Panta instrument. It instructs first-time users on how to use the instrument and serves as a reference for experienced users. Before using the Prometheus Panta instrument, please read this instruction manual carefully, and make sure that the contents are fully understood. -
Page 6: Safety Information
2. Safety information To ensure operation safety, this system must be operated correctly. Carefully read this chapter to fully understand all necessary safety precautions before operating the system. Symbols and descriptions This section describes the safety symbols and descriptions used in this manual, as well as the labels on the system. -
Page 7: Use And Misuse
Do not modify the system in any way. Do not use it for anything other than its intended purpose. Safety instructions CAUTION The Prometheus Panta has to be installed in a way that does not hinder access to the external power supply and its power plug. - Page 8 CAUTION The display pane is made of glass. Broken glass can injure your hands or fingers. CAUTION The sharp edges of the Prometheus Panta thermal element pose a risk of injury to hands and fingers. CAUTION The instrument contains a temperature regulator to control the sample temperature. Some accessible parts of the instrument can reach temperatures of up to 60 °C.
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Page 9: The Prometheus Panta System
3. The Prometheus Panta system General 3.1.1. Intended use The Prometheus Panta system provides fast and sensitive determination and quantification of conformational and colloidal protein stability. For this purpose, it performs nanoDSF (nano-differential scanning fluorimetry), backreflection, and DLS (dynamic light scattering) measurements in glass capillaries. -
Page 10: Technical Information
DLS Laser Wavelength 405 nm ± 5 nm Power 100 mW max. Prometheus Panta Laser classification The device is LASER PRODUCT CLASS 1 Temperature control Temperature control range 15 °C – 95 °C (at 25 °C) Optional High Temperature Upgrade 15 °C –... -
Page 11: Connections For Input And Output
Ethernet connection to host PC Ethernet (PC) Ethernet (LAN) DC Input On/Off Button Capillary Tray Gate Figure 2: Connections on the Prometheus Panta device. Type Function Ethernet To connect the system to the Control PC/LAN via Ethernet cable DC Input... -
Page 12: Legal
Legal 1. NanoTemper Technologies shall not be held liable, either directly or indirectly, for any consequential damage incurred as a result of product use. 2. Prohibitions on the use of NanoTemper Technologies software: o Copying software for purposes other than backup o Transfer or licensing of the right to use software to a third party o Disclosure of confidential information regarding software o Modification of software... -
Page 13: Limited Warranty
Limited warranty Products sold by NanoTemper Technologies, unless otherwise specified, are warrantied to be free of defects in materials and workmanship for a period of one year from the date of shipment. If any defects in the product are identified during this warranty period, NanoTemper Technologies will repair or replace the defective part(s) or product free of charge. -
Page 14: Prometheus Panta Setup
Connect the Prometheus Panta system to power by plugging in the 24V power supply. Connect the Prometheus Panta system to the control PC using the Ethernet connection at the back of the instrument. The system starts upon switching the power switch. -
Page 15: Software Updates
In case of broken capillaries on the capillary tray, use a paintbrush or tissue to carefully remove pieces of glass and to avoid scratching the tray. Other surfaces of the capillary tray can be cleaned in a similar fashion as the outside of the device. Software updates Software updates of the embedded system can only be performed by instructed NanoTemper Technologies personnel and is part of regular maintenance visits. -
Page 16: Prometheus Panta Instrument
5. Prometheus Panta instrument The Prometheus Panta instrument uses nanoDSF technology to detect changes in the fluorescence of the amino acids tryptophan and tyrosine over a wide range of temperatures. The instrument is used to induce thermal unfolding of proteins and to determine thermal unfolding transition temperatures. -
Page 17: Using Prometheus Panta
6. Using Prometheus Panta General Usage Start the Prometheus Panta instrument using the power switch at the back left of the instrument, turn on the control computer and start the software (PR.Panta Control). It will take approximately one minute for the Prometheus instrument to complete hardware initialization. - Page 18 Figure 3: Capillary tray layout and loading for the Prometheus Panta instrument. The removable capillary frame can hold up to 48 capillaries, which are numbered from 1 to 48, starting in the front closest to the user. When filling the capillaries, it is important that the capillaries are filled completely, and that they are centered on the capillary tray.
- Page 19 Figure 4: Manual sample loading of capillary chips. (A) When placed correctly, the plastic chip structure frames the thermal element and the capillaries rest on top of the thermal element. The metal lid is then placed slowly and evenly on top of the ...
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Page 20: Patents And Intellectual Property
7. Patents and intellectual property Prometheus and the nanoDSF technology are patent protected, especially by the following patents: US10488326B2, US10545081B2, US10618051B2, US10900879B2, EP2848310B1, EP3150988B1, including their application and registration in additional countries and jurisdictions. 20/22... - Page 21 8. Transport and disposal Repackaging for transport The Prometheus Panta instrument should be repacked only by trained NanoTemper Technologies personnel to ensure safety and stability during transport. Please store the instrument box for that purpose. If the instrument box was discarded NanoTemper Technologies can provide replacement at the cost of shipment.
- Page 22 Contact NanoTemper Technologies GmbH Global headquarters: Floessergasse 4 81369 Munich Germany Phone: +49 (0)89 4522895 0 Fax: +49 (0)89 4522895 60 info@nanotempertech.com nanotempertech.com NanoTemper®, Prometheus® and nanoDSF® are registered trademarks. V02_20210420 22/22...
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