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7.
Remove tube from colorimeter. Add 8
drops of Ammonia Nitrogen Reagent #1
(V-4797). Cap and mix.
8.
Use 1.0 mL pipet (0354) to add 1.0 mL
of *Ammonia Nitrogen Reagent #2
(V-4798).
9.
Cap and invert to mix. Wait 5 minutes for
full color development. Wipe tube dry.
10. Insert tube into chamber. Close lid.
Select Scan Sample. Record result as
Ammonia-nitrogen.
NOTE: The meter will remember the last scanned blank reading. It is not necessary to scan a blank each time
the test is performed. To use the previous blank reading, instead of scanning a new one, scroll to Scan Sample
and proceed. For the most accurate results, the meter should be blanked before each test and the same tube
should be used for the blank and the reacted sample.
NOTE: For the best possible results, carry a reagent blank through the procedure. After scanning the
blank, perform the test procedure on clear, colorless, distilled or deioized water. Subtract results of regent
blank from all subsequent test results.
CALCULATIONS
To express results as Unionized Ammonia (NH
Unionized Ammonia (NH
To express results as Ionized Ammonia (NH
Ionized Ammonia (NH
Ammonia in water occurs in two forms: toxic unionized ammonia (NH
toxic ionized form, ammonium ion (NH
measures both forms as ammonia-nitrogen (NH
concentration in water. The actual proportion of each compound depends on temperature,
salinity, and pH. A greater concentration of unionized ammonia is present when the pH value and
salinity increase.
):
3
) = ppm Ammonia Nitrogen (NH
3
+
):
4
+
) = ppm Ammonia Nitrogen (NH
4
+
). This test method
4
008 Ammonia-N HR
0.99
ppm
Scan Blank
Scan Sample
12:00:00
-N) x 1.2
3
-N) x 1.3
3
-N) to give the total ammonia-nitrogen
4
5
05:00
001/500
) and the relatively non-
3
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