cytiva Amersham ImageQuant 500 Operating Instructions Manual
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cytiva Amersham ImageQuant 500 Operating Instructions Manual

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Amersham ImageQuant 500
Operating Instructions
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Summary of Contents for cytiva Amersham ImageQuant 500

  • Page 1 Amersham ImageQuant 500 Operating Instructions Original instructions cytiva.com...

  • Page 2: Table Of Contents

    Software keypad ............................. 102 Shutdown ................................103 System settings ....................104 Introduction to the Settings window ....................105 Save location ..............................107 Date and time ..............................110 Network ................................111 Maintenance ..............................112 Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 3 Regulations for North America ....................... 138 9.4.6 Regulatory statements ..........................139 9.4.7 Declaration of Hazardous Substances (DoHS) ................140 9.4.8 Other regulations and standards ......................142 Health and Safety Declaration Form ...................... 143 Index ........................... 145 Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 4: Introduction

    This chapter contains information about this manual and associated user documentation, important user information and intended use of the product. In this chapter Section See page Important user information About this manual Associated documentation Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 5: Important User Information

    • You have read and understood the safety instructions outlined in this Operating Instruction • You should be acquainted with the use of general laboratory equipment and with handling of biological materials • The instrument is installed according to the instructions in this Operating Instruction Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 6: About This Manual

    Notes and tips Note: A note is used to indicate information that is important for trouble-free and optimal use of the product. Tip: A tip contains useful information that can improve or optimize your procedures. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 7: Associated Documentation

    Associated documentation Introduction This section describes the user documentation delivered with the product, and how to find related literature that can be downloaded or ordered from Cytiva. User documentation for ImageQuant The user documentation is listed in the table below.

  • Page 8: Safety Instructions

    Labels and symbols Emergency procedures Important WARNING Before installing, operating or maintaining the product, all users must read and understand the entire contents of this chapter to become aware of the hazards involved. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 9: Safety Precautions

    It is important not to proceed until all stated conditions are met and clearly understood. NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 10 Do not damage the power supply cord by bending, twisting, heating or allowing them to become pinned under the equipment. Using damaged power cords could result in fire or electric shock. If the power supply cords are damaged, contact your local Cytiva representative for replacements. WARNING Access to power switch and power cord with plug.
  • Page 11 Installing and moving the instrument WARNING Use a proper power cord that complies with the local laws and regulations, and is delivered by Cytiva. Do not change power cord to inappropriate one. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 12 In other countries, use the proper power cord delivered separately from the product package by Cytiva. Do not change AC power cord to inappropriate one. WARNING Connect the power supply directly to a grounded wall power outlet.
  • Page 13 CAUTION Do not place heavy objects on the equipment. These may fall and cause injury. CAUTION Do not turn the power off during image capture and saving. This may damage the system files. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 14 Do not attempt to modify the equipment, or fire and electric shock could result. WARNING Do not use excessive amounts of liquids for cleaning the ImageQuant 500, this may result in product malfunction or electric shock. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 15 Do not press too hard onto the surface of the touch screen. This may cause the surface to break and lead to injury. NOTICE Do not use abrasive cleaning materials, such as a scouring pad, for cleaning. This may scratch the surface. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 16: Labels And Symbols

    • Frequency (Hz) Max. Power • Supply Voltage (VAC • Max. Power (W) Alternating current. Protection Degree of protection provided by the enclosure. Class Manufacturing Year (YYYY) and month (MM) of manufacture. Year and Month Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 17 2 Safety instructions 2.2 Labels and symbols Position of labels and symbols Part Description USB label System label FCC and ICES-003 label Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 18: Emergency Procedures

    Turn off the power by pushing the Power switch to the O position on the bottom right hand side of the instrument. Disconnect the power cord from the wall socket. Power failure The following table describes the consequences of a power failure. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 19 • The Power indicator switches off. Computer • The Power indicator on the Instrument control panel displays blue. • The run is interrupted immediately. • If a run was in process, no image data will be saved. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 20: System Description

    About this chapter This chapter contains information on the ImageQuant 500 hardware components,accessories, start screen, and a feature overview. In this chapter Section See page Feature overview Hardware Start screen and capture tab Accessories Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 21: Feature Overview

    • Fluorescence imaging using blue and UV Epi light sources • Photos of stained gels or membranes obtained by white light illumination • Intuitive design with easy to use image analyzing tools Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 22: Hardware

    Password protected USB flash drives are not supported. Body Instrument dark box Door Instrument door Power switch Toggle switch: I Power ON O Power OFF Ethernet port Connector for network cable (Ethernet) Power connector Connector for AC power cord Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 23 • Combined Blue (460 nm) and UV (365 nm) Epi light • White (470 to 635 nm) Epi light Sample chamber Dark compartment where the tray is placed Tray guide Supports and positions the tray Door Instrument door Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 24: Start Screen And Capture Tab

    For more information about the exposure modes, see Section 5.4.1 Chemiluminescence capture, on page 52 Section 5.4.2 Fluorescence capture, on page Capture tab: choose imaging method and start the capture. Library tab: image file handling. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 25 Button for Semi-auto. A short pre-exposure is performed which results in an image. This allows you to select the area of the sample image to focus on. The software automatically calculates the optimal exposure time for the specific area. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 26 87 for more information on how to set the time. Tap the Set exposure time button for fluorescence or chemiluminescence samples (with or without colorimetric markers) to set the exposure time. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 27 The Incremental radio button allows the capture of sequential images. The Set interval time button is used to set the number of repetitions and interval times between exposures to capture sequential images for chemiluminescence samples. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 28: Accessories

    White insert for gels or membranes with colorimetric marker 29005069 and stains Orange filter (560LP) for detection of fluorescence above 560 29005068 Accessories case 29005070 AC power cord (For North America) 80648033 AC power cord (For Europe) 19244801 Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 29: Installation

    This chapter contains information on how to unpack, install, and transport the ImageQuant 500 system. In this chapter Section See page Site requirements Unpacking Instrument set up Connection to network or USB flash drive Moving the ImageQuant 500 system Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 30: Site Requirements

    • At least 20 cm of free space in front of the machine and 10 cm on all other sides • Out of direct sunlight Humidity Operation: 15% to 70%, non-condensing Transportation/Storage: 5% to 95%, non-condensing Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 31: Unpacking

    • Check that all equipment is in the crate according to the packing list. • Check the equipment for damage and document it carefully if found, before starting installation. If any equipment is missing or damage is found, contact your Cytiva representative immediately. Unpacking instruction...
  • Page 32 Open the top of the box and remove the Operating Instructions and the upper padding. Remove the upper packaging of the box from the bottom packaging, then lift the inner box out of the bottom padding and packaging. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 33 Cut the polypropylene bands of the inner box. Remove the top packaging of the box, the accessories box, and the packaging surrounding the instrument. Unpack the accessories box. Continue to the next section to set up ImageQuant 500. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 34: Instrument Set Up

    10 cm on all other sides away from walls or other equipment to ensure adequate cooling. Follow the instructions, to set up ImageQuant 500. Step Action Lift ImageQuant 500 out of the bottom packaging and place it on a workbench. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 35 Romania), use the proper power cord included in the product package. In other countries, use the proper power cord delivered separately from the product package by Cytiva. Do not change AC power cord to an inappropriate one. WARNING Connect the power supply directly to a grounded wall power outlet.
  • Page 36 Ready, see image below. Result: Start-up is now finished. Set the date and time by tapping on Settings and choosing Date & Time. For detailed information, go to Section 6.3 Date and time, on page 110. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 37 4 Installation 4.3 Instrument set up Step Action If desired, go to Section 4.4 Connection to network or USB flash drive, on page to install a network. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 38: Connection To Network Or Usb Flash Drive

    If a Static IP network is used the network details have to be entered manually. The following subsections give the information necessary to connect to a DHCP network and a Static IP network. Open the Network window Follow the instructions below, to open the Network window: Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 39 Connect to a Static IP network on page Connect to a DHCP network Follow the instructions below, to connect to a DHCP network: Step Action Connect the network cable to the Ethernet port on ImageQuant 500. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 40 Connect to a Static IP network Follow the instructions below, to connect to a Static IP network: Step Action Connect the network cable to the Ethernet port on ImageQuant 500. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 41 Section 6.4 Network, on page 111 Keypad on page respectively. Tap the Save button to save the changes. Result: The connection to the Static IP network is established within a couple of seconds. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 42: Moving The Imagequant 500 System

    For transport over longer distances, pack the instrument securely in a protective box. Use the original packing material if possible. Contact Cytiva for information on moving the system over longer distances. Safety precautions WARNING When moving the instrument for maintenance or other purposes, disconnect all cables from wall sockets and separate equipment so that the cables do not pull on the instrument or equipment.
  • Page 43 (Recommended) Test the system performance. Performance tests Performance tests are recommended after moving and re-installing ImageQuant 500 system to confirm that the system is working correctly. Performance tests are described in the ImageQuant 500 User Manual. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 44: Operation

    This chapter describes how to start and operate the ImageQuant 500 system. In this chapter Section See page Start the system Operation overview Place the sample on a tray Capture an image View the images Save the image File handling Software keypad Shutdown Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 45: Start The System

    ImageQuant 500 self-diagnostic starts and a screen with the message 500 initializing... appears. Wait until the start screen is displayed and the message in the lower left corner reads Ready, see image below. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 46 5.1 Start the system Step Action Result: Start-up is now finished. Note: The self diagnostics will finish within 5 minutes, with the exception of connection to a network environment which may take longer. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 47: Operation Overview

    Operation overview Operation flow chart The general flow of operation is as follows: Place a sample on the tray Place the tray in the ImageQuant 500 Capture an image View and save the image Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 48: Place The Sample On A Tray

    DNA stains such as EtBr and SYBR Green. Note: With regards to third party patents related to sample preparation etcetera, we recommend that you consult a lawyer or patent attorney for obtaining appropriate licenses. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 49 Place the gel within the outlines on the DNA tray. Take care not to cover the surrounding area which is used for automatic filter detection and for setting the auto contrast. Open the door of ImageQuant 500. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 50 Place the tray in ImageQuant 500. Use the tray guide to ensure correct placement. The image below shows the protein tray placed in ImageQuant 500. If capturing an image of a fluorescent sample, insert the orange filter. Close the door of ImageQuant 500. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 51: Capture An Image

    • Chemiluminescence (with or without colorimetric marker) • Fluorescence • Colorimetric In this section Section See page 5.4.1 Chemiluminescence capture 5.4.2 Fluorescence capture 5.4.3 Colorimetric capture 5.4.4 Set the exposure and interval time Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 52: Chemiluminescence Capture

    Do not open the door during exposure as this will stop the exposure and the data will not be saved. Chemiluminescence with automatic exposure time Follow the instructions below to perform chemiluminescence capture using automatic exposure time. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 53 5.4.1 Chemiluminescence capture Step Action In the Capture tab, select the Auto button (set as default). If your sample contains a colorimetric marker, then check the Colorimetric marker box. Tap Start exposure for Chemiluminescence. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 54 Selecting Cancel will cancel the exposure and the Capture view will appear. When the exposure is finished the image view appears displaying the image. ImageQuant 500 creates a folder containing image files that can be saved. For details see File formats, on page Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 55 • Select +Marker to see a composite color image of the sample and colorimetric marker images. Tap Save to save the images. For other options see Options after image capture, on page Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 56 Follow the instructions below to perform chemiluminescence capture using semi- automatic exposure time. Step Action In the Capture tab select the Semi-auto button. If your sample contains a colorimetric marker, then check the Colorimetric marker box. Tap Next for Chemiluminescence. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 57 Result: When the pre-exposure is finished a low resolution preview image is displayed. Use the zoom tools and arrows to get an optimal view of the area of interest. Choose to zoom in. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 58 Selecting Cancel cancels the exposure and the Capture view appears. Select: Option 1: Choose , and select a point of interest in the image. Result: The selected point is highlighted with an X. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 59 The selected area is highlighted with a square. Use the right hand side arrow buttons to move the highlighted point or area. Tap Start exposure and wait for the exposure to finish. Result: Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 60 When the exposure is complete, image view appears and the images are displayed. ImageQuant 500 creates a folder containing the created image files which can be saved. For details see File formats, on page Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 61 A selected tab is highlighted with blue text and frame. • Select Sample to see the sample without colorimetric marker. • Select +Marker to see a composite color image of the sample and colorimetric marker images. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 62 Follow the instructions below to perform chemiluminescence capture using manually set exposure time. Step Action In the Capture tab, select the Manual radio button. If your sample contains a colorimetric marker, then check the Colorimetric marker check box. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 63 5.4.1 Chemiluminescence capture Step Action Tap Set exposure time for chemiluminescence. Result: The Set exposure time view opens. Note: Selecting Cancel cancels the exposure and the Capture view appears. Enter a new time. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 64 Tap Start exposure and wait for the exposure to finish. Result: When the exposure is finished the image view appears displaying the image. ImageQuant 500 creates a folder containing image files that can be saved. For details see File formats, on page Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 65 A selected tab is highlighted with blue text and frame. • Select Sample to see the sample without colorimetric marker. • Select +Marker to see a composite color image of the sample and colorimetric marker images. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 66 (colorimetric marker), and one combined color image of the chemiluminescence and white light capture. Chemiluminescence with incremental exposure Follow the instructions below to perform chemiluminescence capture using incremental exposure time. Step Action In the Capture tab, tap the Incremental button. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 67 Step Action Tap the Set interval time button for chemiluminescence. Result: The Set interval time view opens. Set the number of repetitive exposures in the Repeat field using the plus and minus buttons. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 68 Tap Start exposure and images are captured repeatedly for the set time period (or until saturated). Result: The number of images matching the selected repetitions are displayed as they are exposed. A progress bar indicates the time remaining for imaging. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 69 Use the Select button to select the images you wish to save. Then select the Save button. Note: If more than one image is selected, then the Library view will open after saving the images. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 70 • To modify the display settings or view details of the image, select the image tools to the right of the image. See for further instructions. Image tools on page 91 • To discard an image without saving, select Cancel and confirm in the following dialog. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 71: Fluorescence Capture

    The intensity of the image is inadequate after manually. automatic exposure or a known exposure time is required: Fluorescence with automatic exposure Follow the instructions below to perform fluorescence capture using automatic exposure time. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 72 Check the Automatic filter detection disabled box, if the fluorescence sample is larger than the image area (10×15 cm) or if the automatic filter detection fails. Tap the Start exposure button for fluorescence. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 73 When the exposure is complete, image view appears and the image are displayed. ImageQuant 500 creates a folder containing the image files that can be saved. For details, see File formats on page Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 74 In the Capture tab, select the Semi-auto. Check the Automatic filter detection disabled box, if the fluorescence sample is larger than the image area (10×15 cm) or if the automatic filter detection fails. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 75 5 Operation 5.4 Capture an image 5.4.2 Fluorescence capture Step Action Tap the Next button for fluorescence. When the pre-exposure is finished, a low-resolution preview image is displayed. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 76 Use the zoom tools and arrows to get an optimal view of the area of interest. Choose to zoom in. Choose to zoom out. Note: Selecting Cancel will cancel the exposure and the Capture view will appear. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 77 , and select a point of interest in the image. Result: The selected point is highlighted with an X. Choose , and then select two diagonal corners of an area of interest in the image. Result: Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 78 Step Action The selected area is highlighted with a square. Use the right-hand side arrow buttons to move the highlighted point or area. Tap Start exposure and wait for the exposure to finish. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 79 Tap the Save button to save the images. For other options after capturing, Options after image capture on page Fluorescence with manual exposure Follow the instructions below to perform fluorescence capture using manually set exposure time. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 80 Check the Automatic filter detection disabled box, if the fluorescence sample is larger than the image area (10×15 cm) or if the automatic filter detection fails. Tap the Set exposure time for fluorescence. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 81 The Set exposure time view opens. Note: Selecting Cancel will cancel the exposure and the Capture view will appear. Enter the new time. Tap Start exposure and wait for the exposure to finish. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 82 • To save the image, select Save. This will allow images to be reopened later with the image tools. See Section 5.6 Save the image, on page 94 Section 5.7 File handling, on page 97 for further instructions. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 83 • To modify the display settings or view details of the image, select the image tools to the right of the image. See Image tools on page 91, for further instructions. • To discard the image without saving, select Cancel and confirm the following dialog. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 84: Colorimetric Capture

    The images are by default created with automatic exposure time. Automatic colorimetric capture Follow the instructions below to perform colorimetric capture using automatic exposure time. Step Action In the Capture tab, select the Auto button (set as default). Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 85 The exposure begins following a pre-exposure. A progress bar appears on the screen indicating the time remaining of the imaging process, the exposure selected, and the actual exposure time. Note: Selecting Cancel cancels the exposure and the Capture view appears. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 86 • To modify the display settings or view details of the image, select the image tools to Image tools, on page 91 the right of the image. See for further instructions. • To discard the image without saving select Cancel and confirm the following dialog. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 87: Set The Exposure And Interval Time

    Tap Minutes, Seconds, or 1/10 sec to select the unit of time to change. Increase or decrease the exposure time by tapping Tap Reset to set the exposure time to zero. Tap Start exposure to start image capture. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 88 Tap Minutes, Seconds, or 1/10 sec to select the unit of time to change. Increase or decrease the exposure time by tapping Tap Reset to set the exposure time to zero. Tap Start exposure to start image capture. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 89: View The Images

    When the image capture is complete, the images are displayed on the screen. The content shown varies with the capture methods and exposure options. The following illustration and table describe the image result view. Part Function Leaves the image view screen without saving the image. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 90 Displays the Images tools menu. For detailed information about the Images tools menu, go to the Tools menu, see Image tools on page Shows which image tool is selected. In this example the Display tool is selected. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 91 Intensity button: tap on this button to select the intensity tool. Display button: tap on this button to select the display tool. The following table describes each tool in the image tools menu in detail. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 92 The vertical axis represents the number of pixels at a given intensity. The blue and red lines indicate the min/max range of the gray scale in the displayed image. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 93 The Invert button inverts the displayed image, that is, it changes a white background with black bands to a black background with white bands. Note: The Display tool is not available for incremental imaging. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 94: Save The Image

    5 Operation 5.6 Save the image Save the image Save window Part Function Cancel button: Tap on this button to return to the result window without saving. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 95 If the instrument flash drive runs full, the oldest images will be removed automatically when a new image is captured. File formats The image files are stored in the formats described in the table below. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 96 The individual tif images (sample image and marker image) can be used for accurate molecular weight calibration using appropriate analysis software, such as ImageQuant TL. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 97: File Handling

    Copy/Move button: tap on this button to copy or move files. Rename button: tap on this button to rename files. Delete button: tap on this button to delete files. Select all button: tap on this button to select all files. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 98 To delete files from the device: Step Action Tap Delete. Result: The following window opens: Tap Yes to delete the files. Copy or move files To copy or move files: Step Action Tap on Copy/Move. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 99 Use the keypad to change the name of the file. Tap on Done to save the changes. Open files Follow the steps below to open files. Step Action Tap the file to be opened. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 100 Follow the steps below to set up and access web tool. Step Action Open a web browser of choice on the computer. Type in the system's IP address in the browser address field and press enter. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 101 Tap Copy to copy the files. Note: The copied files are downloaded in a zip-file with a name that is automatically generated. The download location and procedure will vary with the chosen web browser's download settings. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 102: Software Keypad

    Changes to the numeric and symbol keypad. Note: Tap on the 'abc' or the 'ABC' button to return to the alphabetic keypad. Cancel button Returns to previous window without saving the changes. 'ABC' button Changes to the uppercase keypad. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 103: Shutdown

    If the ImageQuant 500 instrument is used around the clock, restart the system at least once a day to remove temporary files that may take up unnecessary memory space. To switch off the instrument, push the power switch to the O position. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 104: System Settings

    About this chapter This chapter contains information on the ImageQuant 500 software function, interface and settings. In this chapter Section See page Introduction to the Settings window Save location Date and time Network Maintenance Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 105: Introduction To The Settings Window

    The following instruction provides the necessary information to open the settings window. In the Capture window tap on the Settings icon. Settings window Description Part Function Date & Time button Changes the date and/or time. Network button Changes the network settings. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 106 6 System settings 6.1 Introduction to the Settings window Description Part Function Save locations button Adds, removes, or edits locations and saves images. Maintenance button Updates software or exports a log file. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 107: Save Location

    ImageQuant 500. Step Action Tap on Save locations to open the Save locations window. Result: The following window appears: Tap Add new in the Save locations window. Result: The following window appears: Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 108 Follow the steps below to edit a save location. Step Action Tap on Save locations to open the Save locations window. Tap on Edit. If desired, edit the name for the save location in the Name: field. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 109 For information on how to configure a network, go to Section 4.4 Connection to network or USB flash drive, on page Tap Ok to save the changes. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 110: Date And Time

    Save the changes to Date & time Tap on the Ok button to save the changes to the Date & time. Tap on the Cancel button to leave the Date & time window without saving the changes. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 111: Network

    Change the IP address, the Subnet mask, Router, or Workgroup by tapping the corresponding field. Enter the new address by using the keypad. Tap Done to save the changes, or Cancel to go back to the Network window. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 112: Maintenance

    Update software Follow the steps below to update the software. Step Action Connect the USB flash drive provided by Cytiva containing the update file. Tap the Update software button. Result: The following window appears: Tap Yes to update the software.
  • Page 113 Export log Follow the steps below to export a log. Step Action Tap Export log. Result: A text file is created containing the export log file. Tap Ok to return to the Settings window. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 114: Maintenance

    ImageQuant 500. To ensure that the system works according to specifications, planned maintenance should be performed annually by a Cytvia service engineer, or service personnel authorized by Cytiva. A list of planned maintenance actions is presented in this chapter. NOTICE...

  • Page 115: Safety Precautions

    For continued protection from fire hazard, replace only with same type and rating of fuse. WARNING If a fuse requires repeated replacement, do not continue to use the instrument. Contact an authorized service engineer. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 116: Cleaning Before Planned Service

    Health and safety declaration forms Health and safety declaration forms are available for copying or printing in the Reference information chapter of this manual, or on digital media supplied with the user documentation. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 117: Cleaning The Exterior

    To clean the interior of the instrument, use a non-fluorescent cleaning agent for biochemical use. If using a neutral domestic detergent, the detergent residue may fluoresce when an image is captured. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 118: Cleaning Sample Trays And Filter

    • Mild detergent • Isopropanol • 6% H After cleaning, rinse the tray in water and allow to air dry. To clean the filter use a soft sponge soaked in a non-fluorescent neutral detergent. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 119: Regular Inspections

    • The power cord and supply plug do not become overheated. • The power cord is not damaged in any way. • The ventilation holes in the equipment are free from dust and dirt. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 120: Troubleshooting

    Suggestions of possible solutions are provided. In this chapter Section See page Report an error Problems with the captured image Strange sounds and odors Problems with ImageQuant 500 Problems with ImageQuant 500 Control Software Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 121: Report An Error

    2. Turn off the power to ImageQuant 500 and turn it back on again after about ten seconds. 3. If the error persists and cannot be resolved with this troubleshooting guide, contact your Cytiva representative. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 122: Problems With The Captured Image

    One of the UV LEDs is Turn the power off and contact your broken. Cytiva representative Unevenness is seen on the image The sample tray is not Place the tray correctly at the correctly positioned appropriate tray position Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 123: Strange Sounds And Odors

    The camera emits smoke ImageQuant 500 Turn off the ImageQuant 500 power switch generates a strange odor ImageQuant 500 immediately and contact your Cytiva failure ImageQuant 500 emits representative smoke Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 124: Problems With Imagequant 500

    The hinges are Contact your Cytiva damaged. representative Interlock failure Turn off the power immediately The light source is lit even when the and contact your Cytiva ImageQuant 500 door is opened representative Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 125: Problems With Imagequant 500 Control Software

    Clean the tray fluorescent substances inserted The sample may cover parts Move or change the sample size of the tray that is needed for so it does not cover relevant filter detection parts Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 126: Reference Information

    This chapter lists the technical specifications of ImageQuant 500. The chapter also includes ordering information, and Health and Safety Declaration form for service. In this chapter Section See page Specifications Environmental conditions Recycling information Regulatory information Health and Safety Declaration Form Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 127: Specifications

    105 X 105 mm Greyscale 65 536 levels (16 bit) Exposure time 1/10 seconds to 1 hour Dynamic range 4.8 orders of magnitude Image output Grey scale 16 bit (tif) Color image (jpg) Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 128: Environmental Conditions

    Temperature: 18°C to 28°C (with temperature fluctuation below 10°C humidity conditions per hour or lower) Humidity: 15% to 70% RH (no dew condensation) Note: When the above conditions cannot be satisfied, take appropriate action. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 129 Overvoltage category Transient overvoltage category II Rated pollution applied Pollution Degree 2 Noise 70 dB(A) or lower Fast Lmax ≤60dB(A), and average Leq ≤ 54dB(A) Degrees of protection IP21 provided by enclosure Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 130: Recycling Information

    Decontaminate the ImageQuant 500 Cabinet and system cabinet following appropriate procedures depending on what type of environment the unit was located in. Follow local and/or national/federal requirements for disposal of the ImageQuant 500 Cabinet and the system cabinet. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 131 9.3 Recycling information Step Action Dispose of electronic components as specified by local regulations depending on material used in the construction of the components. Follow local and/or national/federal requirements for disposal of the electronic components. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 132: Regulatory Information

    European Union and European Economic Area 9.4.3 Great Britain 9.4.4 Eurasian Economic Union Евразийский экономический союз 9.4.5 Regulations for North America 9.4.6 Regulatory statements 9.4.7 Declaration of Hazardous Substances (DoHS) 9.4.8 Other regulations and standards Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 133: Contact Information

    The table below summarizes the required manufacturing information. Requirement Information Name and address of manufacturer Cytiva Sweden AB Björkgatan 30 SE 751 84 Uppsala Sweden Telephone number of manufacturer + 46 771 400 600 Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 134: European Union And European Economic Area

    • used according to the Operating Instructions or user manuals, and • used in the same state as it was delivered, except for alterations described in the Operating Instructions or user manuals. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 135: Great Britain

    • Used according to the Operating Instructions or user manuals, and • Used in the same state as it was delivered, except for alterations described in the Operating Instructions or user manuals. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 136: Eurasian Economic Union

    Telephone: +7 499 609 15 50 E-mail: rucis@cytiva.com Информация о производителе и импортере В следующей таблице приводится сводная информация о производителе и импортере, согласно требованиям Технических регламентов Таможенного союза и (или) Евразийского экономического союза. Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 137 Member States of the Customs Union of the Eurasian Economic Union Данный знак о Евразийском соответствии указывает, что изделие одобрено для использования на рынках государств-членов Таможенного союза Евразийского экономического союза Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 138: Regulations For North America

    Note: The user is cautioned that any changes or modifications not expressly approved by Cytiva could void the user’s authority to operate the equipment. This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC Rules.

  • Page 139: Regulatory Statements

    When used in a residential environment, there is a concern of radio interference. 유의사항 A급 기기 (업무용 방송통신 기자재) 이 기기는 업무용환경에서 사용할 목적으로 적합성평가를 받 은 기기 로서 가정용 환경에서 사용하는 경우 전파간섭의 우려가 있습 니다. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 140: Declaration Of Hazardous Substances (Dohs)

    This product must not be disposed of as unsorted municipal waste, and must be collected separately and handled properly after decommissioning. 有害物质的名称及含量 Name and Concentration of Hazardous Substances 产品中有害物质的名称及含量 Table of Hazardous Substances’ Name and Concentration Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 141 Indicates that this hazardous substance contained in at least one of the homogeneous materials used for this part is above the limit requirement in GB/T 26572 • Data listed in the table represents best information available at the time of publication. Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 142: Other Regulations And Standards

    The safety of the LED light sources in this equipment has been assessed by risk analysis in accordance with EN 61010-1. ImageQuant 500 is equipped with three LED light sources: Wavelength Blue Epi light 460 nm UV Epi light 365 nm White Epi light 470 to 635 nm Amersham ImageQuant 500 Operating Instructions 29655392 AA...

  • Page 143: Health And Safety Declaration Form

    Service Ticket #: To make the mutual protection and safety of Cytiva service personnel and our customers, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the servicing of your equipment, complete this checklist and present it to the Service Engineer upon arrival.
  • Page 144 To make sure the mutual protection and safety of Cytiva personnel, our customers, transportation personnel and our environment, all equipment must be clean and free of any hazardous contaminants before shipping to Cytiva. To avoid delays in the processing of your equipment, complete this checklist and include it with your return.

  • Page 145: Index

    File formats, 95 File name, 95 Filter, 28 Place the sample, 48 Fluorescence, 71, 74, 79 Product return or servicing, 144 automatic exposure, 71 Purpose of this manual, 6 manual exposure, 79 semi-automatic exposure, 74 Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 146 Keypad, 102 Save window, 94 Specifications, 127 Tray, 28 DNA tray, 28 Protein tray, 28 Typographical conventions, 6 UK, 135 conformity, 135 UKCA, 135 marking, 135 Unpacking, 31 Web tool, 100 White insert, 28 Amersham ImageQuant 500 Operating Instructions 29655392 AA...
  • Page 147 Page intentionally left blank...
  • Page 148 A copy of this Standard Software End-User License Agreement is available on request. All goods and services are sold subject to the terms and conditions of sale of the supplying company operating within the Cytiva business. A copy of those terms and conditions is available on request. Contact your local Cytiva representative for the most current information.

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