Hanna Instruments HI 84533 Instruction Manual
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Hanna Instruments HI 84533 Instruction Manual

Formol number minititrator for fruit juices and wines analysis
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Instruction Manual
HI 84533
FORMOL NUMBER
MINITITRATOR
for Fruit Juices and Wines analysis
w w w. h a n n a i n s t . c o m
1

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  • Page 1 Instruction Manual HI 84533 FORMOL NUMBER MINITITRATOR for Fruit Juices and Wines analysis w w w. h a n n a i n s t . c o m...

  • Page 2: Table Of Contents

    ELECTRODE CONDITIONING AND MAINTENANCE ..............41 ACCESSORIES ........................42 WARRANTY ........................43 All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

  • Page 3: Preliminary Examination

    Pump calibrations, performed with the provided Hanna reagents, assure the accuracy of the instrument. The instrument comes with a preprogrammed method for Formol measurements in fruit juices and wines. The HI 84533 performs the analysis and all the necessary calculations, with a simple and effective user interface.
  • Page 4 8.1 and 8.2 pH), in order to ensure it stops at set point pH value, the titration is performed using a low concentrated titrant. The HI 84533 is a potentiometric titrator that use a pH electrode for endpoint. The volume of the titrant dispensing, necessary to reach the endpoint, is used to calculate the formol number. The...

  • Page 5: Specifications

    SPECIFICATIONS Titrator Range Low Range: meq/L 2.14 to 28.57 as N meq% 0.21 to 2.85 as N mg/L 30.0 to 400.0 as N High Range: meq/L 21.7 to 71.4 as N meq% 2.14 to 7.14 as N mg/L 300 to 1000 as N Resolution Low Range: 0.01 meq/L;...

  • Page 6: Principle Of Operation

    A potentiometric acid-base titration employs a pH electrode to establish the endpoint of the titration. The HI 84533 minititrator is designed to determine the formol number of the wines and fruit juices. This instrument uses two titrant solutions in acidity neutralization steps (for shorting of the titration time) and one low concentration titrant solution in the step of the formol number determination.

  • Page 7: Functional Description

    FUNCTIONAL DESCRIPTION OVERHEAD VIEW 1) Titrant bottle 2) Liquid Crystal Display (LCD) 3) Keypad 4) Electrode holder 5) Dispensing tube FRONT VIEW REAR VIEW 6) Temperature probe 7) pH Electrode 8) Beaker 9) Dosing pump 10) Power switch 11) Power adapter 12) USB connector (PC interface) 13) USB connector (Storage interface) 14) Temperature connector...
  • Page 8 KEYPAD FUNCTION ESC - used to leave the current screen and to return either to the previous screen or to the main screen. In Setup, exits a parameter without changing the value. - used to modify the parameters’ values, to scroll the information displayed while viewing a help screen or to move between the options from the instrument’s Setup CAL - used to access the Electrode and Pump calibration options HELP - used to access/exit the instrument’s contextual help...

  • Page 9: Titrator Startup

    5) Stirrer and reading status 6) Main reading information 7) pH temperature compensation mode (Manual or Automatic) 8) Temperature reading DOSING PUMP The dosing pump is based on a valve that automatically moves the titrant between the titrant bottle and syringe when filling the syringe and between the syringe and sample when dispensing.

  • Page 10: Setup Menu

    SETUP MENU The titrator’s setup menu may be accessed from the main screen (meter or titrator) by pressing the MENU key, then Setup. A list of setup parameters will be displayed with currently configured setting. While in the setup menu it is possible to modify the instrument’s operation parameters. The ARROW keys permit the user to scroll the setup parameters.
  • Page 11 Temperature Unit °C, °F or K. Press the virtual option key to change the option. Time Press the Modify key to change the time and time format. Press Format to switch between 12 hour (am/pm) and 24 hour mode. Press to highlight the value to be modified.
  • Page 12 Error Beep Select Enable to activate or Disable to deactivate the Error Beep function. If enabled, a beep will be heard when an error condition occurs. Decimal Separator This option allows the user to select the symbol used for a decimal separator. LCD Contrast This option is used to set the display’s contrast.
  • Page 13 pH endpoint Press Modify to change the pH endpoint value. The default value is 8.3 pH. Use the ARROW keys to select the value. Press Accept to confirm or ESC to return to the setup menu without saving the changes. Language Press the corresponding virtual option key to change the language.

  • Page 14: Guide To Display Codes

    Restore Factory Settings Press Select to restore the factory settings. Press Yes to confirm the restore process or No to return without restoring. Press ESC to return to the setup menu. GUIDE TO DISPLAY CODES This screen appears when the instrument is turned on during the initialization process.
  • Page 15 This error message appears when the pump is not working properly. Check the tubing, valve and syringe. Press Restart to try again. This screen appears when the titrator is in calibration mode. Press Pump to calibrate Pump. Press Electrode to calibrate pH electrode. PUMP CALIBRATION MESSAGES Pump calibration is initiated by pressing the Start key.
  • Page 16 This screen appears when the stirrer is not working properly. Check the stir bar and beaker content. Press Restart to try again. This error message appears when the pump is not working properly. Check the tubing, valve and syringe. Press Restart to try again. pH CALIBRATION MESSAGES pH calibration mode.
  • Page 17 This screen is displayed when the instrument in manual preparation phase and is waiting to adjust the pH adding drops of HI 84533-62. When the manual pH adjustment is finished please continue to start the automatically fine adjustment. This screen is displayed when the instrument is automatically adjusting the pH to the endpoint value.

  • Page 18: Electrode Preparation

    This error message appears when the input reading (pH or temperature) exceeds the input limits. If the pH or temperature values are blinking they are out of range. This screen appears when the sample concentration is out of range. This screen appears when the stirrer is not working properly.

  • Page 19: Electrode Calibration Procedure

    4.01, 7.01, 8.20 and 10.01 pH. For a single point calibration any of the four buffers may be used, 8.20 pH is recommended. Note: The HI 84533 will not accept other pH buffers for calibration. • Pour small quantities of selected buffer solutions into clean beakers. For accurate calibration use two beakers for each buffer solution, the first one for rinsing the electrode and the second one for calibration.
  • Page 20 Point 1 calibration • The default buffer selected will be 7.01 if endpoint was set to 7.00 pH or else 8.20 pH will be selected. If necessary, press the ARROW keys in order to select a different buffer value. • The (unstable measurement) symbol will be shown on the display until the reading becomes stable.
  • Page 21 • The (unstable measurement) symbol will be shown on the display until the reading becomes stable. • When the reading is stable and close to the selected buffer, the (unstable measurement) symbol will disappear and the Confirm key will become active. •...
  • Page 22 • If the measured offset isn’t within the preset limits (±45 mV), the meter will display the message “Buffer Contaminated” alternatively with ”Electrode Dirty/Broken”. • If the computed slope isn’t within the preset limits, the meter will display the message “Wrong Slope”.

  • Page 23: Ph Buffer Temperature Dependence

    Note: • To clear a previous calibration and to return to the default value, press Clear at any time after entering calibration mode. If Clear is invoked during the first calibration point the instrument returns to the measurement mode. • The Clear key is displayed only if a previous calibration exists. pH BUFFER TEMPERATURE DEPENDENCE Temperature has an effect on pH.

  • Page 24: Dosing Pump Installation

    DOSING PUMP INSTALLATION To install the dosing pump follow the procedure below: • Extend the plunger on the 5 mL syringe to its maximum volume. • Place the syringe in the dedicated spot on the top of the meter (1). •...
  • Page 25 Note: The aspiration tube must be inserted in the titrant bottle. The dosing tip must be placed over a rinse beaker. • To prime the burette, select Prime option from Titration mode. • Adjust the rinses number by pressing the keys and press Start.

  • Page 26: Pump Calibration Procedure

    • Verify the electrode has been calibrated in 8.20 pH buffer. Sample preparation: Use a clean tip and 2000 µL automatic pipette to precisely add the appropriate amount of HI 84533-55 Calibration Standard to a clean beaker as indicated below: Low Range - 2 mL...
  • Page 27 • Insert the dosing tip into the titrant tube sleeve. IT IS CRITICAL THAT THE TIP BE IMMERSED APPROXIMATELY 0.25 CM (0.1") INTO THE SOLUTION BEING TITRATED. • Press Continue to begin the calibration and Stop to abort it. • At the end of the calibration, ”Calibration Completed“ appears on display.

  • Page 28: Titration Procedure

    TITRATION PROCEDURE • For best accuracy, before taking any measurement, ensure that the pump is calibrated on the selected range following the “Pump Calibration Procedure” (see page 26). Note: Verify that the instrument has been calibrated (pH and pump) before performing any titrations.
  • Page 29 • Press Continue to begin the titration and Stop to abort it. • The instrument will start the manual pretitration procedure to adjust the sample pH to set value. • Add drop by drop from HI 84533-62 waiting to have stable pH between drops until the “Rough pH adjustment ended”...
  • Page 30 • Press Continue and the syringe will be refilled for final titration. • Add 4 mL of HI 84533-61 Formol Base reagent to sample. • Insert the dosing tip into the titrant tube sleeve. IT IS CRITICAL THAT THE TIP BE IMMERSED APPROXIMATELY 0.25 CM (0.1") INTO THE SOLUTION BEING TITRATED.
  • Page 31 • Press LOG to record the concentration value into the instrument’s memory. A message will be displayed for a few seconds indicating the amount of free log space. Up to 200 log samples can be recorded in the instrument’s memory. •...
  • Page 32 VIEW/DELETE TITRATOR RECORDED DATA Press MENU then Recall to access the Titrator logs. When an external USB storage device is connected, the Export key is displayed. It saves the meter and titrator logs in two text format files on the storage device. Press Meter or Titrator to view the respective logs.
  • Page 33 Press Plot to visualize the titration curve or ESC to return to the previous screen. On the titration curve, the endpoint volume and pH are displayed. The titration data (Total Titrant Volume on the x- axis and pH on the y-axis) can be scanned through with the dotted line by using the ARROW keys. To zoom on the titration curve press Zoom.

  • Page 34: Ph Measurement

    If a calibration hasn’t been performed, the message “Not Calibrated” will be displayed. pH MEASUREMENT The HI 84533 can be used as a pH meter for direct measurements. Verify that the instrument has been calibrated before taking pH measurements. Set the instrument to pH meter.
  • Page 35 In order to take pH measurements, follow the next steps: • Submerge the pH electrode 2 cm (0.8’’) and the temperature probe into the sample to be tested and stir gently. Make sure the PTFE junction is completely submersed. Allow time for the electrode to stabilize.
  • Page 36 VIEW/DELETE RECORDED pH DATA To view or delete previously logged pH records, press MENU then Recall to access the pH logs. When an external USB storage device is connected, the Export key is displayed. It saves the meter and titrator logs in two text format files on the storage device. Press Meter or Titrator to view the respective logs.
  • Page 37 Deleting a single record will renumber the list of records. If the pH log is empty, the message “No records available!” will be displayed. pH METER GLP INFORMATION The pH meter GLP screens displays the last pH calibration data. To view this information, press MENU key then GLP. Press Electrode to view information regarding electrode calibration.

  • Page 38: Pc Interface And Data Transfer

    PC INTERFACE AND DATA TRANSFER Data stored on the meter with the LOG function during pH/mV measurement and titrations can be transferred from the meter to a USB stick using the Export function from the log recall menu. Two text files are transferred on the USB stick. These files can be used for further analysis on a PC. The logged data can also be transferred from the instrument to the PC using a USB cable.

  • Page 39: Troubleshooting Guide

    TROUBLESHOOTING GUIDE e h t c e l o r t p i t p s e / e s c x e s s e e v i e l c g n i t u l n o i y t r i c e l o r t...
  • Page 40 o r B n e k e l e o r t c . e d c e h l c / k n a e e h t e l e o r t c . e d r e t f a r t i n o i t e h t...

  • Page 41: Electrode Conditioning And Maintenance

    ELECTRODE CONDITIONING AND MAINTENANCE STORAGE PROCEDURE To assure a quick response time, the glass bulb should be kept moist and not allowed to dry out. Replace the solution in the protective cap with a few drops of HI 70300 or HI 80300 Storage Solution.

  • Page 42: Accessories

    ACCESSORIES REAGENTS HI 84533-50 Titrant Solution (230 mL) HI 84533-55 Calibration Standard (120 mL) HI 84533-60 Additional Reagent (30 mL) HI 84533-61 Formol base Reagent (230 mL) HI 84533-62 Adjustment Reagent (30 mL) pH CALIBRATION SOLUTIONS HI 7004M Buffer solution pH 4.01 (230 mL) HI 7007M Buffer solution pH 7.01 (230 mL)

  • Page 43: Warranty

    WARRANTY HI 84533 is guaranteed for two years against defects in workmanship and materials when used for it’s intended purpose and maintained according to instructions. Electrodes and probes are guaranteed for six months. This warranty is limited to repair or replacement free of charge.
  • Page 44 Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA Technical Support for Customers Tel. (800) 426 6287 Fax (401) 765 7575 E-mail tech@hannainst.com www.hannainst.com Local Sales and Customer Service Office MAN84533 10/13 Printed in ROMANIA...

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