Hanna Instruments HI84529 Instruction Manual
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Hanna Instruments HI84529 Instruction Manual

Titratable acidity mini titrator & ph meter for dairy products
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HI84529
Titratable Acidity
Mini Titrator & pH Meter
for Dairy Products

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Summary of Contents for Hanna Instruments HI84529

  • Page 1 HI84529 Titratable Acidity Mini Titrator & pH Meter for Dairy Products...
  • Page 2 If you need additional technical information, do not hesitate to e‑mail us at tech@hannainst.com or view our worldwide contact list at www.hannainst.com. All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

  • Page 3: Table Of Contents

    TABLE OF CONTENTS 1. Preliminary Examination ..................... 4 2. Specifications ........................5 3. General Description & Intended Use ..................7 4. Principle of Operation ......................9 5. Functional Description ....................... 10 6. Titrator Startup ......................... 13 7. Setup Menu ........................14 8.

  • Page 4: Preliminary Examination

    1. PRELIMINARY EXAMINATION Remove the instrument and accessories from the packaging and examine it carefully. For further assistance, please contact your local Hanna Instruments Office or email us at tech@hannainst.com. Each HI84529 mini titrator is supplied with: • FC260B pH electrode •...

  • Page 5: Specifications

    2. SPECIFICATIONS Low Range %I.a.: 0.01 to 0.20 °SH: 0.4 to 8.9 °D: 1.0 to 20.0 °TH: 1.1 to 22.2 Range High Range %I.a.: 0.1 to 2.0 °SH: 4.4 to 88.9 °D: 10 to 200 °TH: 11.1 to 222.2 %I.a.: 0.01 (Low Range) / 0.1 (High Range) °SH: 0.1 Resolution °D: 0.1 (Low Range) / 1 (High Range)
  • Page 6 Power Supply 12 Vdc power adapter Dimensions 235 x 200 x 150 mm (9.2 x 7.9 x 5.9”) Weight 1.9 kg (67.0 oz.) Required Reagents HI84529-50 Titrant Low Range 20 HI84529-51 Titrant High Range 20 HI84529-52 Titrant Low Range 50 HI84529-55...

  • Page 7: General Description & Intended Use

    The instrument benefits from Hanna Instruments’ many years of experience as a manufacturer of quality analytical instrumentation. A clear and well-designed user interface makes the instrument intuitive and simple to use.
  • Page 8 Low 20 / High 20 to titrate 20 mL or 20 g samples that are diluted with twice its volume of deionized a distilled water. The HI84529 uses methods based on AOAC International and Standard Methods for the Examination of Dairy Products. Both of these methods...

  • Page 9: Principle Of Operation

    This endpoint for this reaction can be determined visually using a color indicator (i.e. phenolphthalein), however this endpoint becomes very objective in opaque and colored samples. The HI84529 removes this issue by titrating to a fixed pH endpoint that can be set by the user. The...

  • Page 10: Functional Description

    5. FUNCTIONAL DESCRIPTION Front View 1. Dosing pump valve 2. Aspiration tube 3. Syringe 4. Titrant bottle 5. Dispensing tube 6. Reference electrode 7. Temperature probe 8. pH electrode 9. Electrode holder Top View 1. Titrant bottle 2. Dispensing tube 3.
  • Page 11 Rear View 1. Power switch 2. USB connector (PC interface) 3. USB connector (storage interface) 4. Power adapter 5. BNC electrode connector 6. Reference electrode connector 7. Temperature probe connector Keypad Description The keypad contains 8 direct keys and 3 functional keys with the following functions: Press the functional key to select the virtual option displayed above it on the LCD.
  • Page 12 Display Indicators During the instrument’s operation a set of information are displayed on the LCD. 1. Current time and instrument mode information (pH meter or Titrator) 2. pH electrode condition 3. Instrument status 4. Virtual option keys 5. Stirrer and reading status 6.

  • Page 13: Titrator Startup

    6. TITRATOR STARTUP This is a general outline of the steps required to perform a titration. The following topics are expanded upon in each section that follows. • Place the instrument on a flat table. Do not place the instrument in direct sun light. •...

  • Page 14: Setup Menu

    7. SETUP MENU The titrator’s setup menu may be accessed from the main screen (meter or titrator) by pressing the MENU key, then Setup. A list of setup parameters will be displayed with currently configured setting. While in the Setup menu, it is possible to modify the instrument’s operation parameters. The ARROW keys permit the user to scroll the setup parameters.
  • Page 15 Calibration Timeout Disabled or 1 to 7 days This option is used to set the number of days before the pH calibration expired warning message is displayed. Press Modify to access the calibration timeout screen. Use the ARROW keys to select the value. Press Select to confirm or ESC to return to the setup menu without saving the changes.
  • Page 16 Date Press the Modify key to change the date and date format. Press Format to cycle between the available date formats. Press to highlight the value to be modified. Use the ARROW keys to change the value. Press Accept to confirm the new value or ESC to return to the setup. Key Beep Select Enable to activate or Disable to deactivate the Key Beep function.
  • Page 17 Use the ARROW keys or to increase/decrease the value. Press Accept to confirm the value or ESC to return to the setup menu. LCD Backlight Press Modify to change the backlight value. The default value is 3. Use the ARROW keys or to increase/decrease the backlight level.
  • Page 18 Tutorial Enable or Disable the Tutorial. This helpful tool offers additional information during calibration and titration. Meter Information Press Select to view the firmware version, language version, mV factory calibration date and time and temperature factory calibration date and time. Press ESC to return to the setup menu.

  • Page 19: Guide To Display Codes

    8. GUIDE TO DISPLAY CODES This screen appears when the instrument is turned on during the initialization process. Titration screen display. Titration screen when a titration is in progress. Prime burette screen. Prime burette screen when the dosing system is running. This error message appears when the pump is not working properly.

  • Page 20: Pump Calibration Messages

    8.1. PUMP CALIBRATION MESSAGES Pump calibration is initiated by pressing the Start key. This screen appears while pump calibration is in progress. Press ESC or Stop key to return to the Pump Calibration screen. This error message appears during pump calibration when the endpoint can not be reached and the maximum amount of titrant is exceeded.

  • Page 21: Ph Calibration Messages

    8.2. pH CALIBRATION MESSAGES pH calibration mode. When the reading has stabilized, press Confirm to accept the calibration or Clear to restore the default calibration. The “Wrong Buffer” message is displayed when the pH value is outside of the acceptable range. Clean the electrode by following the Cleaning Procedure and/or check the buffer concentration before continuing the pH calibration.

  • Page 22: Titration Messages

    8.3. TITRATION MESSAGES This screen is displayed when the instrument is in titration mode. Press Start to begin a titration, Meter to enter pH meter mode or Prime to enter into the prime function. The titration result, expressed as concentration in selected unit, is displayed automatically at the end of the titration.

  • Page 23: Electrode Preparation

    9. ELECTRODE PREPARATION Remove the electrode protective cap. Do not be alarmed if any salt deposits are present. This is normal with electrodes and they will disappear when rinsed with distilled/deionized water. During transport, tiny bubbles of air may have formed inside the glass bulb. The electrode cannot function properly under these conditions.
  • Page 24 Measurement Place pH electrode and reference electrode into electrode holder and connect the electrodes to the instrument. Rinse the pH and reference electrodes with distilled or deionized water. Immerse the pH and reference electrodes 1.5 cm (0.6”) in the sample and stir gently for a few seconds. For a faster response and to avoid cross-contamination of the samples, rinse the electrodes with a few drops of the solution to be tested, before taking measurements.

  • Page 25: Electrode Calibration Procedure

    8.30 and 10.01 pH. For one-point calibration any of the four buffers may be used, 8.30 pH is recommended. Note: The HI84529 will not accept other pH buffers for calibration. • Pour small quantities of selected buffer solutions into clean beakers. For accurate calibration use two beakers for each buffer solution, the first one for rinsing the electrode and the second one for calibration.
  • Page 26 • The (unstable measurement) symbol will be shown on the display until the reading becomes stable. • When the reading is stable and close to the selected buffer, the (unstable measurement) symbol will disappear and the Confirm key will become active. •...
  • Page 27 • After the second calibration point has been confirmed, press ESC to exit without performing the third calibration point. Three-Point Calibration • Remove the electrode holder with electrodes from the top of the beaker. • Place the third beaker with calibration buffer in the beaker holder. Rinse the electrodes in a beaker containing the third buffer rinsing solution.
  • Page 28 • If the computed slope isn’t within the preset limits, the meter will display the message “Wrong Slope”. If the slope is too high, the symbol will be displayed. If the slope is too low, the symbol will be displayed. •...

  • Page 29: Ph Buffer Temperature Dependence

    11. pH BUFFER TEMPERATURE DEPENDENCE Temperature has an effect on pH. The calibration buffer solutions are affected by temperature changes to a lesser degree than normal solutions. During calibration, the instrument will automatically calibrate to the pH value corresponding to the measured or set temperature. TEMP pH BUFFERS ºC...

  • Page 30: Dosing Pump Installation

    12. DOSING PUMP INSTALLATION To install the dosing pump follow the procedure below: 1. Extend the plunger on the 5 mL syringe to its maximum volume. 2. Unscrew the syringe-fixing nut and remove the o-ring. Front view 3. Place the syringe in the dedicated spot on the top of the meter.

  • Page 31: Dosing Pump Prime Procedure

    13. DOSING PUMP PRIME PROCEDURE Prime cycle should be performed: • if there is no titrant in the tip • whenever the dosing system tubes are replaced • whenever a new bottle of titrant is used • before starting a pump calibration •...
  • Page 32 • The number of syringe rinses can be set between 1 and 5 (at least three rinses are recommended to ensure that the air bubbles are completely removed). • To pause the prime process press the Pause key; to continue press the Continue key. To stop the prime process press the Stop key.

  • Page 33: Pump Calibration Procedure

    1.1 to 22.2 11.1 to 222.2 • Verify the electrode has been calibrated in 8.30 pH buffer. • Ensure the pump is primed with the correct titrant for the selected range (HI84529-50 Low Range 20 Titrant or HI84529-52 Low Range 50 Titrant, HI84529-51 High Range 20 Titrant).
  • Page 34 • Press Start, wait for the syringe to refill. • Place the stir bar in the beaker and put the beaker in the mini titrator top. • Place the probe holder on the top of the beaker and secure it by turning clockwise. •...
  • Page 35 Notes: If temperature probe is not connected, Manual Temperature Compensation is used and MTC appears on the right side of the screen. If Automatic Temperature Compensation is in use, the ATC appears on the right side of the screen. If an erroneous situation is encountered during the calibration, an error message is displayed and the calibration can be restarted by pressing Restart.

  • Page 36: Titration Procedure

    • Select the corresponding measurement range: Low 20, Low 50 or High 20. • Select the corresponding titration type: by volume or by mass. • Ensure the pump is primed with the correct titrant for the selected range (HI84529-50 Low Range 20 Titrant or...
  • Page 37 • For 20 g and 20 mL samples add deionized or distilled water to the 60 mL mark. • Place the stir bar in the beaker and put the beaker in the mini titrator top. • Place the probe holder on the top of the beaker and secure it by turning clockwise. •...
  • Page 38 • The titration curve can be visualized during a titration by pressing Plot ON. Press Plot OFF or ESC to exit this mode. • At the end of the titration, the concentration is displayed in the selected unit. The titration curve can be viewed by pressing Plot ON.

  • Page 39: Tips For An Accurate Measurement

    • This screen appears when the stirrer is not working properly. Check the stir bar and beaker content. Press Restart to try again. • This error message appears when the pump is not working properly. Check the tubing, valve and syringe.
  • Page 40 If the saved concentration was out of range, the “<” or “>” symbols are displayed in front of the reading. Press Delete to delete the selected record from the memory. Press Del.All to delete all records. Press Info to see detailed information about the highlighted record. The selected record data and the titration curve data file name are displayed.

  • Page 41: Titrator Glp Information

    If Delete or Del.All is pressed ,the instrument will ask for confirmation. Press Yes to delete the record or No to return to the previous screen. Deleting a single record will renumber the list of records. If the titrator log is empty, the message “No records available!” will be displayed. 15.3.

  • Page 42: Ph Measurement

    16. pH MEASUREMENT HI84529 can be used as a pH meter for direct measurements. Verify that the instrument has been calibrated before taking pH measurements. Set the instrument to pH meter. From titrator mode press Meter until pH units are displayed.

  • Page 43: View / Delete Recorded Ph Data

    If measurements are taken successively in different samples, it is recommended to rinse the electrodes thoroughly with deionized or distilled water and then with some of the next sample, to prevent cross-contamination. The pH reading is affected by temperature. In order to have accurate pH measurements, the temperature effect must be compensated for.

  • Page 44: Ph Meter Glp Information

    Use ARROW keys when is displayed to scroll between the records. Press ESC to return to the previous screen. If Delete or Del.All is pressed, the instrument will ask for confirmation. Press Yes to delete the record or No to return to the previous screen without deleting. Deleting a single record will renumber the list of records.

  • Page 45: Pc Interface & Data Transfer

    17. PC INTERFACE & DATA TRANSFER Data stored on the meter with the LOG function during pH/mV measurement and titrations can be transferred from the meter to a USB stick using the Export function from the log recall menu. Two text files are transferred on the USB stick.

  • Page 46: Electrode Conditioning & Maintenance

    • General: Soak in Hanna Instruments HI7061 HI8061 General cleaning solution for approximately ½ hour. • Milk deposits: Soak in Hanna Instruments HI70640 Cleaning solution for milk deposits for approximately ½ hour (pH half cell only). • Protein: Soak in Hanna Instruments...

  • Page 47: Troubleshooting Guide

    19. TROUBLESHOOTING GUIDE SYMPTOMS PROBLEM SOLUTION Slow response/excessive Dirty pH or Soak the electrode tip HI70640 solution drift. reference electrode. for 30 minutes and follow the cleaning procedure. Refill reference electrode with new fill solution. Reading fluctuates up and Clogged/dirty junction. Clean the pH electrode.
  • Page 48 SYMPTOMS PROBLEM SOLUTION At startup, the meter One of the keys is Check the keyboard or contact the vendor. displays the Hanna stuck. Instruments logo permanently. “Error xx” message is Internal error. Power off the meter and then power it on displayed.

  • Page 49: Accessories

    20. ACCESSORIES REAGENTS HI84529-50 Titrant solution for Low Range 20, 120 mL HI84529-51 Titrant solution for High Range 20 , 120 mL HI84529-52 Titrant solution for Low Range 50, 120 mL HI84529-55 Pump calibration standard, 230 mL pH CALIBRATION SOLUTIONS HI7004M pH 4.01 buffer solution, 230 mL...

  • Page 50: Certification

    If service is required, contact your local Hanna Instruments Office. If under warranty, report the model number, date of purchase, serial number (see engraved on the back of the instrument) and the nature of the problem.
  • Page 51 Hanna Instruments reserves the right to modify the design, construction or appearance of its products without advance notice.
  • Page 52 World Headquarters Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA www.hannainst.com MAN84529 Printed in ROMANIA...

This manual is also suitable for:

Hi84529u-01

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