Hanna Instruments HI84502 Instruction Manual
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Hanna Instruments HI84502 Instruction Manual

Titrable total acidity mini titrator & ph meter for wine analysis
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HI84502
Titrable Total Acidity
Mini Titrator & pH Meter
for Wine Analysis

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Summary of Contents for Hanna Instruments HI84502

  • Page 1 HI84502 Titrable Total Acidity Mini Titrator & pH Meter for Wine Analysis...
  • Page 2 If you need additional technical information, do not hesitate to e‑mail us at tech@hannainst.com or view our worldwide contact list at www.hannainst.com. All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

  • Page 3: Table Of Contents

    TABLE OF CONTENTS 1. Preliminary Examination ..................... 4 2. Specifications ........................5 3. General Description & Intended Use ..................6 4. Principle of Operation ......................8 5. Functional Description ......................9 6. Titrator Startup ......................... 12 7. Setup Menu ........................13 8.

  • Page 4: Preliminary Examination

    1. PRELIMINARY EXAMINATION Remove the instrument and accessories from the packaging and examine it carefully. For further assistance, please contact your local Hanna Instruments Office or email us at tech@hannainst.com. Each HI84502 mini titrator is supplied with: • HI1048B/50 pH electrode •...

  • Page 5: Specifications

    0 to 50 °C (32 to 122 °F); max 95% RH non-condensing Power Supply 12 Vdc power adapter Dimensions 235 x 200 x 150 mm (9.2 x 7.9 x 5.9”) Weight 1.9 kg (67.0 oz.) Required Reagents HI84502-50 Titrant HI84502-55 Calibration standard...

  • Page 6: General Description & Intended Use

    Hanna Instruments’ years of experience as manufacturer of analytical instruments. The instrument incorporates a simple and reliable dosing pump which ensures high dosing reproducibility. Pump calibrations, performed with the provided Hanna Instruments reagents, assure the accuracy of the instrument. The instrument comes with a preprogrammed method for Total Titratable Acidity measurements in wine.
  • Page 7 Total Acidity directly affects the color and flavor of wine and, depending on the style of the wine, is sought in a perfect balance with the sweet and bitter sensations of other components. Too much acidity makes wine tart and sharp; too little makes wines flat, flabby and uninteresting. Proper acidity in wine is what makes it refreshing and an ideal accompaniment to food.

  • Page 8: Principle Of Operation

    4. PRINCIPLE OF OPERATION The determination of total acids in wine is made according to a neutralization reaction, that is the reaction between the acids found in wine and a base. Titratable acidity is measured on a degassed sample at the endpoint of 8.20. The results are expressed as g/L tartaric acid.

  • Page 9: Functional Description

    5. FUNCTIONAL DESCRIPTION Front View 1. Dosing pump valve 2. Aspiration tube 3. Titrant bottle 4. Dispensing tube 5. Syringe 6. Temperature probe 7. pH electrode 8. Electrode holder 9. Beaker Top View 1. Titrant bottle 2. Dispensing tube 3. Electrode holder 4.
  • Page 10 Rear View 1. Power switch 2. USB connector (PC interface) 3. USB connector (storage interface) 4. Power adapter 5. BNC electrode connector 6. Reference electrode connector 7. Temperature probe connector Keypad Description The keypad contains 8 direct keys and 3 functional keys with the following functions: Press the functional key to select the virtual option displayed above it on the LCD.
  • Page 11 Display Indicators During the instrument’s operation information is displayed on the LCD. 1. Current time and instrument mode information (pH meter or Titrator) 2. pH electrode condition 3. Instrument status 4. Virtual option keys 5. Stirrer and reading status 6. Main reading information 7.

  • Page 12: Titrator Startup

    6. TITRATOR STARTUP This is a general outline of the steps required to perform a titration. The following topics are expanded upon in each section that follows. • Place the instrument on a flat table. Do not place the instrument in direct sun light. •...

  • Page 13: Setup Menu

    7. SETUP MENU The titrator’s setup menu may be accessed from the main screen (meter or titrator) by pressing the MENU key, then Setup. A list of setup parameters will be displayed with currently configured setting. While in the setup menu it is possible to modify the instrument’s operation parameters. The ARROW keys permit the user to scroll the setup parameters.
  • Page 14 pH Endpoint 7.00 or 8.20 Press the displayed virtual option key to change the option. pH Resolution 0.1 or 0.01 Press the displayed virtual option key to change the option. Temperature Unit °C, °F or K Press the virtual option key to change the option. Time Press the Modify key to change the time format.
  • Page 15 Date Press the Modify key to change the date format. Press Format to cycle between the available date formats. Press to highlight the value to be modified. Use the ARROW keys to change the value. Press Accept to confirm the new value or ESC to return to the setup. Key Beep Select Enable to activate or Disable to deactivate the Key Beep function.
  • Page 16 LCD Contrast This option is used to set the display’s contrast. Press Modify to change the display’s contrast. The default value is 8. Use the ARROW keys or to increase/decrease the value. Press Accept to confirm the value or ESC to return to the setup menu. LCD Backlight Press Modify to change the backlight level.
  • Page 17 Meter Information Press Select to view the firmware version, language version, mV factory calibration date and time, temperature factory calibration date and time, method version. Press ESC to return to the setup menu. Restore Factory Settings Press Select to restore the factory settings. Press Yes to confirm the restore process or No to return without restoring.

  • Page 18: Guide To Display Codes

    8. GUIDE TO DISPLAY CODES This screen appears when the instrument is turned on during the initialization process. Titration screen display. Titration screen when a titration is in progress. Prime burette screen. Prime burette screen when the dosing system is running. This error message appears when the pump is not working properly.

  • Page 19: Pump Calibration Messages

    8.1. PUMP CALIBRATION MESSAGES Pump calibration is initiated by pressing the Start key. This screen appears while pump calibration is in progress. Press ESC or Stop key to return to the Pump Calibration screen. This error message appears during pump calibration when the endpoint can not be reached and the maximum amount of titrant is exceeded.

  • Page 20: Ph Calibration Messages

    8.2. pH CALIBRATION MESSAGES pH calibration mode. When the reading has stabilized press Confirm to accept the calibration or Clear to restore the default calibration. The “Wrong Buffer” message is displayed when the pH value is outside of the acceptable range. Clean the electrode by following the Cleaning Procedure and/or check the buffer concentration before continuing the pH calibration.

  • Page 21: Titration Messages

    8.3. TITRATION MESSAGES This screen is displayed when the instrument is in titration mode. Press Start to begin a titration, Meter to enter pH meter mode or Prime to enter into the prime function. The titration result, expressed as concentration of tartaric acid in g/L (ppt), is displayed automatically at the end of the titration.

  • Page 22: Electrode Preparation

    9. ELECTRODE PREPARATION Preparation Procedure Remove the electrode protective cap. Do not be alarmed if any salt deposits are present. This is normal with electrodes and they will disappear when rinsed with distilled/deionized water. During transport tiny bubbles of air may have formed inside the glass bulb. The electrode cannot function properly under these conditions.

  • Page 23: Electrode Calibration Procedure

    7.01 pH calibration point is recommended, or else use 8.20 pH. Note: The HI84502 will not accept other pH buffers for calibration. • Pour small quantities of selected buffer solutions into clean beakers. For accurate calibration use two beakers for each buffer solution, the first one for rinsing the electrode and the second one for calibration.
  • Page 24 One-Point Calibration • The default buffer selected will be 7.01 if endpoint was set to 7.00 pH or else 8.20 pH will be selected. If necessary, press the ARROW keys in order to select a different buffer value. • The (unstable measurement) symbol will be shown on the display until the reading becomes stable.
  • Page 25 • Press Confirm to confirm the calibration. • The calibrated value will be shown on the display and the third expected buffer value will be automatically selected. • After the second calibration point has been confirmed, press ESC to exit without performing the third calibration point.
  • Page 26 • If the computed slope isn’t within the preset limits, the meter will display the message “Wrong Slope”. If the slope is too high the symbol will be displayed. If the slope is too low the symbol will be displayed. •...

  • Page 27: Ph Buffer Temperature Dependence

    11. pH BUFFER TEMPERATURE DEPENDENCE Temperature has an effect on pH. The calibration buffer solutions are affected by temperature changes to a lesser degree than normal solutions. During calibration the instrument will automatically calibrate to the pH value corresponding to the measured or set temperature. TEMP pH BUFFERS °C...

  • Page 28: Dosing Pump Installation

    12. DOSING PUMP INSTALLATION To install the dosing pump follow the procedure below: 1. Extend the plunger on the 5 mL syringe to its maximum volume. 2. Unscrew the syringe-fixing nut and remove the o-ring. Front view 3. Place the syringe in the dedicated spot on the top of the meter.

  • Page 29: Dosing Pump Prime Procedure

    13. DOSING PUMP PRIME PROCEDURE Prime cycle should be performed: • if there is no titrant in the tip • whenever the dosing system tubes are replaced • whenever a new bottle of titrant is used • before starting a pump calibration •...
  • Page 30 • The number of syringe rinses can be set between 1 and 5 (at least three rinses are recommended to ensure that the air bubbles are completely removed). • To pause the prime process press the Pause key; to continue press the Continue key. To stop the prime process press the Stop key.

  • Page 31: Pump Calibration Procedure

    • Verify the electrode has been calibrated in 8.20 pH buffer or in 7.01 pH buffer. Sample preparation: Use a clean tip and the 2000 µL automatic pipette to precisely add the appropriate amount of HI84502-55 Calibration Standard to a clean beaker as indicated below: Low Range – 4 mL High Range –...
  • Page 32 • Place the stir bar in the beaker and put the beaker in the mini titrator top. • Place the probe holder on the top of the beaker and secure it by turning clockwise. • Rinse the pH electrode with deionized water and immerse into the sample until the PTFE reference junction is completely submerged.
  • Page 33 If an erroneous situation is encountered during the calibration, an error message is displayed and the calibration can be restarted by pressing Restart. Prepare a new standard, rinse electrode, temperature probe and dosing tip and try again. If the calibration doesn’t complete and the max titrant volume of titrant is reached, an error message will be displayed.

  • Page 34: Titration Procedure

    15. TITRATION PROCEDURE • For best accuracy, before taking any measurement, ensure that the pump is calibrated on the selected range following the PUMP CALIBRATION PROCEDURE description. Note: Verify that the instrument has been calibrated (pH and pump) before performing any titrations.
  • Page 35 • Place the probe holder on the top of the beaker and secure it by turning clockwise. • Rinse the pH electrode with deionized water and immerse into the sample until the PTFE reference junction is completely submerged. Be sure that the tip of the electrode is not hitting the stir bar. •...
  • Page 36 • At the end of the titration the concentration is displayed in g/L as tartaric acid. The titration curve can be viewed by pressing Plot ON. Press Plot OFF to exit this mode. • Press LOG to record the concentration value into the instrument’s memory. A message will be displayed for a few seconds indicating the amount of free log space.

  • Page 37: Tips For An Accurate Measurement

    • This error message appears when the pump is not working properly. Check the tubing, valve and syringe. Press Restart to try again. 15.1. TIPS FOR AN ACCURATE MEASUREMENT The instructions listed below should be followed carefully to ensure measurements are conducted with the highest possible accuracy and precision.
  • Page 38 Press Delete to delete the selected log from the memory. Press Del.All to delete all records. Press Info to see detailed information about the highlighted record. The selected record data and the titration curve data file name are displayed. When a USB storage device is connected, the Export key is displayed. It saves the titration curve data as a text file on the storage device using the displayed file name.

  • Page 39: Titrator Glp Information

    If the titrator log is empty, the message “No records available!” will be displayed. 15.3. TITRATOR GLP INFORMATION Press MENU then GLP. From this screen it is possible to select the Electrode or the Pump GLP. Press Pump to view the pump’s last calibration time, date and slope. If a calibration hasn’t been performed, the message “Not Calibrated”...

  • Page 40: Ph Measurement

    16. pH MEASUREMENT HI84502 can be used as a pH meter for direct measurements. Verify that the instrument has been calibrated before taking pH measurements. Set the instrument to pH meter. From titrator mode press Meter until pH units are displayed.

  • Page 41: View / Delete Recorded Ph Data

    • If the pH reading is less than -2.00 pH or greater than 16.00 pH, the closest full-scale value will be displayed blinking. During pH measurements with stirrer on, the stirrer icon will be displayed. In case of a stirrer malfunction, the stirrer will stop and the stirrer icon will start blinking.
  • Page 42 If the saved mV/pH measurements are out of range, the “<” or “>” symbols are displayed in front of the reading. Use the ARROW keys to scroll the list of records. Press Delete to delete the selected log from the memory. Press Del.All to delete all the records.

  • Page 43: Ph Meter Glp Information

    16.2. pH METER GLP INFORMATION The pH meter GLP screens displays the last pH calibration data. To view this information, press MENU key then GLP. Press Electrode to view information regarding electrode calibration. GLP contains a set of information regarding electrode calibration. The following items are included in electrode GLP: the time and date of the last calibration, offset, slope, electrode condition, calibration timeout and the calibration buffers.

  • Page 44: Pc Interface & Data Transfer

    17. PC INTERFACE & DATA TRANSFER Data stored on the meter with the LOG function during pH/mV measurement and titrations can be transferred from the meter to a USB stick using the Export function from the log recall menu. Two text files are transferred on the USB stick.

  • Page 45: Electrode Conditioning & Maintenance

    18. ELECTRODE CONDITIONING & MAINTENANCE Reference fill Hole Reference Wire Potection Sleeve Sensing Wire Reference Junction Platinum Ring Glass Bulb Glass Body ORP Electrode Preparation Procedure Remove the protective cap of the pH electrode (HI1048B/50). Do not be alarmed if salt deposits are present.
  • Page 46 Refill the reference chamber with fresh electrolyte (HI7082). Allow the electrode to stand upright for 1 hour. Follow the Storage Procedure above. pH Cleaning Procedure • Wine deposits: Soak in Hanna Instruments HI70635 cleaning solution for 15 minutes • Wine stains: Soak in Hanna Instruments...

  • Page 47: Troubleshooting Guide

    19. TROUBLESHOOTING GUIDE SYMPTOMS PROBLEM SOLUTION Slow response/excessive drift. Dirty pH electrode. Soak the electrode tip in HI7061 cleaning solution for 30 minutes. Refill with fresh fill solution. Reading fluctuates up and Clogged/dirty junction. Soak the electrode tip in HI7061 down (noise).
  • Page 48 25.0 g/L blinking. range. At startup the meter displays One of the keys is stuck. Check the keyboard or contact the the Hanna Instruments logo vendor. permanently. “Error xx” message is Internal error. Power off the meter and then power displayed.

  • Page 49: Accessories

    20. ACCESSORIES REAGENTS HI84502-50 Titrant solution, 230 mL HI84500-55 Calibration standard, 120 mL pH CALIBRATION SOLUTIONS HI7004M pH 4.01 buffer solution, 230 mL HI7007M pH 7.01 buffer solution, 230 mL HI70082M pH 8.20 buffer solution, 230 mL HI7010M pH 10.01 buffer solution, 230 mL...

  • Page 50: Certification

    If service is required, contact your local Hanna Instruments Office. If under warranty, report the model number, date of purchase, serial number (see engraved on the back of the instrument) and the nature of the problem.
  • Page 51 Hanna Instruments reserves the right to modify the design, construction or appearance of its products without advance notice.
  • Page 52 World Headquarters Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA www.hannainst.com MANMAN84502 Printed in ROMANIA...

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