Related Manuals for Shel lab SCO6AD-2
Summary of Contents for Shel lab SCO6AD-2
- Page 1 HIGH-HEAT DECONTAMINATION CO INCUBATOR 220 – 240 Voltage Installation - Operation Manual SCO6AD-2...
- Page 2 Warning: This product contains chemicals, including triglycidyl isocyanurate, known to the State of California to cause cancer as well as birth defects or other reproductive harm. For more information, go to www.P65Warnings.ca.gov. ¡Advertencia! Este producto contiene sustancias químicas, incluido el triglicidil isocianurato, que el estado de California sabe que causa cáncer, así...
- Page 3 HIGH-HEAT DECONTAMINATION CO INCUBATOR Part Number (Manual): 4861713 Revision: September 23, 2019 SHEL LAB is a brand of Sheldon Manufacturing, INC, an ISO 9001 certified manufacturer. Safety Certifications These units are CUE listed by TÜV SÜD as air jacketed CO...
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Page 4: Table Of Contents
TABLE OF CONTENTS INTRODUCTION ................................. 7 Read this Manual ..................................7 Safety Considerations and Requirements ........................7 Contacting Assistance ................................8 Manufacturing Warranty ..............................8 Engineering Improvements..............................8 Gas Supply ..................................9 Reference Sensor Devices ..............................10 RECEIVING YOUR UNIT ............................11 Inspect the Shipment ................................ - Page 5 Calibrate the CO Display ..............................52 UNIT SPECIFICATIONS ............................57 Weight ......................................57 Dimensions .....................................57 Capacity ....................................57 Chamber CO Performance .............................. 58 Chamber Temperature ..............................58 Power ...................................... 58 PARTS AND CONSUMABLES ..........................59 Components..................................59 Ordering ....................................60 Accessories ................................... 60 P a g e...
- Page 6 TABLE OF CONTENTS P a g e...
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Page 7: Introduction
INTRODUCTION Thank you for purchasing a SHEL LAB product. We know you have many choices in today’s competitive marketplace when it comes to constant temperature equipment. We appreciate you choosing ours. We stand behind our products and will be here for you if you need us. -
Page 8: Contacting Assistance
Therefore, some changes, modifications, and improvements may not be covered in this manual. If your unit’s operating characteristics or appearance differs from those described in this manual, please contact your SHEL LAB dealer or customer service representative for assistance. -
Page 9: Co 2 Gas Supply
INTRODUCTION Note: A CO gas regulator must be purchased separately from the incubator. UPPLY Supply Required! The incubator must be connected to a carbon dioxide gas supply system in order to establish and maintain a CO -enriched incubation chamber atmosphere. The supply can be a building CO gas system or a supply cylinder (tank). -
Page 10: Reference Sensor Devices
INTRODUCTION EFERENCE ENSOR EVICES Must be purchased separately Reference sensor devices or a combined device are required for calibrating the incubator temperature and CO displays. Reference devices must meet the following standards: Accurate to at least 0.1°C • Temperature Accurate to at least 0.1% CO •... -
Page 11: Receiving Your Unit
RECEIVING YOUR UNIT NSPECT THE HIPMENT When a unit leaves the factory, safe delivery becomes the responsibility of the carrier. • Damage sustained during transit is not covered by the manufacturing defect warranty. • Save the shipping carton until you are certain that the unit and its accessories function •... -
Page 12: Orientation Image
RECEIVING YOUR UNIT RIENTATION MAGE SCO6AD-2 Alarm Dry Contact, Sample Port, USB-Style Serial Port Back of Unit Fuse in Power Inlet 2nd Fuse in Holder In Ports Incubation Chamber Control Panel Side Access Port with Copper Filter (Side of Unit) -
Page 13: Recording Data Plate Information
RECEIVING YOUR UNIT ECORDING LATE NFORMATION The data plate contains the incubator model number and serial number. Tech Support will need this information during any support call. Record it below for future reference. The data plate is located on the right side of the unit, above the copper-filtered access port •... - Page 14 RECEIVING YOUR UNIT 14 | P a g e...
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Page 15: Installation
INSTALLATION NSTALLATION HECKLIST For installing the unit in a workspace. Pre-Installation Check that the required ambient conditions and ventilation spacing for the incubator are met, page 16. Unit dimensions may be found on page 52 • Check for performance-disrupting heat and cold sources in the environment, page 16 ... -
Page 16: Required Ambient Conditions
INSTALLATION EQUIRED MBIENT ONDITIONS These units are intended for use indoors, at room temperatures between 15°C and 30°C (59°F and 86°F), at no greater than 80% Relative Humidity (at 25°C / 77°F). Operating outside these conditions may adversely affect its incubator temperature stability and effective operating range. EQUIRED LEARANCES These clearances are required to provide air flows sufficient for ventilation and cooling. -
Page 17: Power Source Requirements
INSTALLATION OWER OURCE EQUIREMENTS When selecting a location for the unit, verify each of the following requirements is satisfied. Power Source The power source for the unit must match the voltage and match or exceed the ampere requirements listed on the unit data plate. These units are intended for 220 – 240V 50/60 Hz applications at 6 amps. -
Page 18: Lifting And Handling
INSTALLATION IFTING AND ANDLING The unit is heavy. Use appropriate lifting devices that are sufficiently rated for these loads. Follow these guidelines when lifting the unit. Lift the unit only from its bottom surface. • Doors, handles, and knobs are not adequate for lifting or stabilization. •... -
Page 19: Installation - Clean And Disinfect
INSTALLATION NSTALLATION LEAN AND ISINFECT Cleaning and disinfecting the incubation chamber and shelving components now reduces the risk of contamination. The chamber was cleaned and disinfected at the factory, however, the unit may have been exposed to contaminants during shipping. Remove all protective wrappings from shelving components prior to cleaning. - Page 20 INSTALLATION ONNECT TO THE UPPLY Note: See page 9 for CO supply requirements. to Chamber Optional Tubing Kit with Inline HEPA Filter Connects to 3/16” (4.76mm) ID Tubing Single Source Dual Source “IN” “IN” Connect the supply source to the incubator. Optional: If connecting a second supply cylinder, do so now.
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Page 21: Access Port Stopper
INSTALLATION CCESS TOPPER Verify the port stopper is installed in the access port on the right side of the unit. • The stopper must be installed on the outside of the unit. • The unit will not meet its temperature performance specifications or maintain a CO setpoint concentration without the stopper installed. - Page 22 INSTALLATION 22 | P a g e...
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Page 23: Graphic Symbols
GRAPHIC SYMBOLS Your incubator comes provided with graphic symbols on its exterior surfaces. These identify hazards and the function of the adjustable components, as well as important notes in the user manual. Symbol Definition Consult the user manual. Consulter le manuel d'utilisation Temperature display Indique l'affichage de la température Over Temperature Limit system... - Page 24 GRAPHIC SYMBOLS 24 | P a g e...
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Page 25: Control Panel Overview
CONTROL PANEL OVERVIEW Control Panel Power Switch Power is supplied when the switch is in the ( I ) on position. Over Temperature Limit This graduated dial sets the Over Temperature Limit system heating cut off point. The OTL system helps prevents unchecked heating of the incubation chamber in the event the main temperature control system fails. - Page 26 CONTROL PANEL OVERVIEW Display Labeled Set CO this display shows the concentration of CO as a percentage of the incubation chamber atmosphere. The display has a range of OFF (0%) to 20% and an accuracy of 0.1%. The display shows “LO” until the CO sensor registers a concentration in the chamber greater than 0%.
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Page 27: Operation
OPERATION HEORY OF PERATION The SCO6AD-2 CO incubator provides a constant-temperature CO -enriched environment suitable for mammalian cell sample cultivation and other incubation applications. The unit features an automated high-heat, chamber decontamination cycle that does not require the removal of the CO sensor or other electronic components. - Page 28 OPERATION The processor employs proportional-integral-derivative analytic feedback-loop functions when measuring and controlling the CO concentration. Injection lengths are proportional to the difference between the measured concentration and the gas setpoint. The frequency of injections is derived from the rate of change in the difference. Integrator feedback slows the rate of injection as the concentration approaches the setpoint, which helps prevent overshoots.
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Page 29: Put The Incubator Into Operation
OPERATION UT THE NCUBATOR INTO PERATION Carry out the following steps and procedures to put the unit into operation after installing it in a new workspace environment. Attach the power cord that came with the unit to the power inlet receptacle on the side of the incubator. - Page 30 OPERATION Put the Incubator into Operation (Continued) Allow the unit to run 8 hours to heat soak and achieve a stable chamber temperature. The manufacturer recommends allowing the unit to run • overnight. Set the Over Temperature Limit. See page 37. The incubator must be heated and stable at your •...
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Page 31: Humidifying The Incubator
OPERATION UMIDIFYING THE NCUBATOR The incubation chamber must be humidified to achieve its stated temperature uniformity specification. Humidification raises the relative humidity of the chamber to 95%, helping to slow the drying of samples in breathable sample containers such as Petri plates. Installation Secure the copper, antimicrobial token in the pan using the pan clip. -
Page 32: Set The Temperature Setpoint
OPERATION ET THE EMPERATURE ETPOINT 1. Set OTL control to its maximum setting, if not already set to max. Turning the OTL all the way to the right (clockwise) prevents the heating • cutoff system from interfering with this procedure. Jump to the Temperature Setpoint Adjustment mode ⁰C ⁰C... -
Page 33: Muting The Audible Temperature Alarm
OPERATION Note: The Temperature Deviation Alarm is disabled during high-heat decontamination cycles. UTING THE UDIBLE EMPERATURE LARM Audible and visual deviation alarms activate if the incubation chamber temperature deviates by 1°C above or below the temperature setpoint. The low deviation audible alarm has a delay of 15 minutes to prevent the low audible from sounding each time the doors are opened, causing a short- lived drop in temperature. -
Page 34: Start A Flow Of Co
OPERATION TART A LOW OF The gas supply must be connected to the incubator per the instructions in the Installation chapter. Gas Cylinder(s) Open the cylinder valve(s) to supply CO to the regulator. Set cylinder regulator flow setting(s). Single source: Set the regulator to deliver 15 - 20 Pounds per Square Inch (psi) to •... -
Page 35: Set The Co
OPERATION ET THE ETPOINT The incubator comes from the factory with the CO setpoint set to Off. Set the setpoint percentage to the gas concentration required by your study or production protocol. Jump to the CO Setpoint Adjustment mode Set CO Set CO Setpoint Adjustment Mode Initial Setpoint... -
Page 36: Muting The Audible Co Alarm
OPERATION UTING THE UDIBLE LARM A visual deviation indicator alarm illuminates if the chamber CO level deviates 1% above or below the CO setpoint. An audible alarm sounds immediately for a high deviation. The low deviation audible alarm sounds only after the visual low indicator light has been continually illuminated for 15 minutes. -
Page 37: Set The Over Temperature Limit
OPERATION Note: Test the OTL system at least once per year for functionality. ET THE EMPERATURE IMIT This procedure sets the OTL heating cutoff point approximately 1⁰C above the current incubation chamber temperature. The unit must be operating stabilized at your application temperature for at least 1 hour prior to setting the mechanical OTL cutoff system. -
Page 38: Decontamination Cycle
OPERATION Note: The incubator was run through a decontamination cycle at the factory. However, the manufacturer cannot guarantee the unit was not been exposed to contaminants during shipping. ECONTAMINATION YCLE The incubator will not inject CO gas during the cycle. The CO sensor is protected from exposure to high temperatures does not need to be removed. - Page 39 OPERATION Decontamination Cycle Perform the following steps to launch the cycle, then restore the incubator to normal operation mode upon completion. 1. Take the incubator out of normal operation mode. a. Press and hold the Operation Selector Switch until the amber Decon Cycle indicator begins flashing. The green Normal Operation indicator will extinguish •...
- Page 40 OPERATION Decontamination Cycle (Continued) The Cooldown stage begins when the 2-hour Soak timer reaches 0 (zero). The amber Decon Cycle and green Normal • Operation indicators flash alternatingly during the Cooldown. The CO shows “CdN”, indicating that the cycle has •...
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Page 41: Aborting The Decontamination Auto Cycle
OPERATION BORTING THE ECONTAMINATION YCLE This procedure forces an active decontamination cycle to enter cooldown mode. Allow the incubator to cool to 50°C before opening the chamber door. 1. Abort the Cycle. a. Press and hold both the Decontamination Cycle and Operation Selector switches. -
Page 42: Load The Incubator
OPERATION OAD THE NCUBATOR The chamber must be operating stabilized at your application temperature prior to loading samples. Place items on the shelves inside the incubation chamber as evenly spaced as possible. Proper spacing allows for maximum air circulation and a high degree of temperature uniformity. Leave 1 inch (25mm) between sample containers and the chamber walls. - Page 43 OPERATION Optional Outputs The SCO6AD may be ordered from the factory as a special quote (SQ) unit equipped with a pair of 4 to 20 milliamp analog outputs describing the current chamber temperature and CO levels. The outputs are transmitted through two jack ports on the right side of the incubator. The port connects to a standard audio jack and cable (male mono phono plug, ¼...
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Page 44: Condensation And The Dew Point
OPERATION ONDENSATION AND THE OINT Relative humidity inside the incubator chamber should never be allowed to exceed 95%. Exceeding this threshold will likely result in condensation, possible leaks around the incubator, and may cause corrosion damage if allowed to continue for any significant length of time. Condensation takes place whenever the humidity level in the incubator chamber reaches the dew point. -
Page 45: User Maintenance
USER MAINTENANCE Warning: Disconnect the unit from its power supply prior to performing maintenance or services. Avertissement: Débranchez cet appareil de son alimentation électrique avant d'effectuer la maintenance ou les services. LEANING AND ISINFECTING If a hazardous material or substance has spilled in the unit chamber, immediately initiate your site Hazardous Material Spill Containment protocol. -
Page 46: Minimizing Contamination Exposure
USER MAINTENANCE Disinfecting For maximum effectiveness, disinfection procedures are typically performed after cleaning. Keep the following points in mind when disinfecting the unit. Turn off and disconnect the unit to safeguard against electrical hazards. • Disinfect the unit chamber using commercially available disinfectants that are non-corrosive, •... -
Page 47: Gas Lines And Hepa Filters
USER MAINTENANCE HEPA F INES AND ILTERS The manufacturer recommends replacing in-line gas HEPA filters once per year or “IN” when a filter is noticeably discolored. HEPA filters are directional and must be installed facing in the correct direction. • The word “IN”... -
Page 48: Calibrate The Temperature Display
USER MAINTENANCE ALIBRATE THE EMPERATURE DISPLAY Note: This procedure requires a temperature reference device. Please see the Reference Sensor Device entry on page 10 for the device requirements. Temperature calibrations are performed to match the incubator temperature display to the actual air temperature inside the incubation chamber. - Page 49 USER MAINTENANCE Temperature Stabilization The incubator air temperature must be stable in order to perform an accurate calibration. Allow the incubator to operate undisturbed with the chamber door shut for at least 8 hours • when first putting the unit into operation in a new environment. To be considered stabilized, the incubator chamber must operate at your calibration •...
- Page 50 USER MAINTENANCE Temperature Calibration Continued Place the display in its temperature calibration mode. a. Press and hold both the UP and DOWN temperature arrow buttons simultaneously for approximately 5 seconds. b. Release the buttons when the temperature display shows the letters “C O”. The display will begin flashing the current temperature display value.
- Page 51 USER MAINTENANCE Compare the reference device reading with the chamber Reference Device temperature display again. If the reference device and the chamber temperature • display readings are the same or the difference falls within the range of your protocol, the incubator is now calibrated for temperature.
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Page 52: Calibrate The Co Display
USER MAINTENANCE ALIBRATE THE ISPLAY Note: This procedure requires a gas reference device. Please see the Reference Sensor Devices entry on page 10 for the device requirements. Gas calibrations are performed to match the incubator CO display to the actual CO concentration inside the incubation chamber. - Page 53 USER MAINTENANCE Gas Stabilization The CO concentration in the incubation chamber must be given time to stabilize in order to perform an accurate calibration. The unit must be thermally stable, having operated at your application temperature for at • least 8 hours prior to starting the gas calibration. After turning on a flow of CO to the chamber and setting the CO setpoint, allow the...
- Page 54 USER MAINTENANCE Calibration Continued Place the incubator CO display in its calibration mode. a. Press and hold both the UP and DOWN temperature arrow buttons simultaneously for approximately 5 seconds. b. Release the buttons when the temperature display shows the letters “C O”. The display will then begin flashing the current CO display value.
- Page 55 USER MAINTENANCE Calibration Continued Compare the reference device reading with the incubator CO Reference Device display again. If the reference device and the incubator display readings • are the same or the difference falls within the range of your protocol, the incubator is now calibrated for CO -OR- ...
- Page 56 USER MAINTENANCE 56 | P a g e...
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Page 57: Unit Specifications
UNIT SPECIFICATIONS The SCO6AD-2 High-Heat Auto-Decontamination Incubator is a 220 – 240 voltage unit. Please refer to the unit data plate for individual electrical specifications. Technical data specified applies to units with standard equipment at an ambient temperature of 25°C and at nominal voltage. -
Page 58: Chamber Co Performance
0 – 20% Less than 5 minutes HAMBER EMPERATURE Range Uniformity Stability ± 0.25ºC at 37ºC ± 0.1°C @ 37°C Ambient +5ºC to 50ºC OWER Model AC Voltage Amperage Frequency SCO6AD-2 220 – 240 50/60 Hz 58 | P a g e... -
Page 59: Parts And Consumables
PARTS AND CONSUMABLES OMPONENTS Description Part Number Description Part Number Access Port Stopper Humidification Pan 7750565 995-00015 Brass Tubing Fitting Copper Token, (Gas Tubing Kit Humidification Pan Component) 5800529 3100520 Power Cord 250 volt, 10 Amp, 2.5m Viewing Door Gasket (8ft), EU1-16P, CEE7/7 3450644... -
Page 60: Ordering
PARTS AND CONSUMABLES RDERING If you have the Part Number for an item, you may order it directly from Sheldon Manufacturing by calling 1-800-322-4897 extension 3. If you are not certain that you have the correct Part Number, or if you need that specific item, please contact Sheldon Technical Support for help at 1-800-322-4897 extension 4 or (503) 640-3000. - Page 61 PARTS AND CONSUMABLES 61 | P a g e...
- Page 62 P.O. Box 627 Cornelius, OR 97113 support@sheldonmfg.com sheldonmanufacturing.com 1-800-322-4897 (503) 640-3000 FAX: 503 640-1366...
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