BestScope BS-7020 Instruction Manual

Inverted fluorescence microscope

Advertisement

Quick Links

Inverted Fluorescence Microscope
BS-7020
Instruction Manual
To ensure correct operation, please read this manual thoroughly before using the
microscope and keep it near the product for easy reference.

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the BS-7020 and is the answer not in the manual?

Questions and answers

Summary of Contents for BestScope BS-7020

  • Page 1 Inverted Fluorescence Microscope BS-7020 Instruction Manual To ensure correct operation, please read this manual thoroughly before using the microscope and keep it near the product for easy reference.
  • Page 2: Table Of Contents

    BestScope International Limited BS-7020 CONTENTS User notice ........................3 I. Nomenclature ......................6 2. Installation ......................... 6 2-1 Installation Diagram ..................7 2-2 Installing steps ....................8 2-2-1 Installing and replacing the lamp ............8 2-2-2 Installing the lamp house ............... 9 2-2-3 Mounting the objectives ................
  • Page 3 BestScope International Limited BS-7020 5-2-2 Centering the light annulus ..............25 6. Microscope photography and video ................ 26 6-1 Microscope video.................... 26 6-1-1 Selecting the light path ................. 26 6-1-2 Installing the video set ................. 26 6-1-3 Focus ..................... 26 6-2 Microscope photography ................
  • Page 4: User Notice

    BS-7020 User notice 1. Application BS-7020 Inverted Biological microscope is the dedicated microscope for biological and medical areas, applied in the field of microscopic measurement, health agencies, laboratories, research institutes and universities and other units for biology, genetics, immunology, chemistry, environment protection, oceanography, pharmacology, bacterial observation, education and professional studies.
  • Page 5 BestScope International Limited BS-7020 5. Before replacing the lamp bulb or fuse, turn the main switch① to the “O”(off) position, then cut off the power. If the lamp is on, or soon after it has been turned off, it is hot and will cause serious burns, please do the replacement after it cool down completely.
  • Page 6 BestScope International Limited BS-7020 4. Safety Symbols Symbol Explanation Indicate that the surface becomes hot, and should not be touched with bare hands. Before use, carefully read the instruction manual. improper use could result in personal injury to the user and/or damage to the equipment.
  • Page 7: Nomenclature

    Protection barrier Mechanical ruler Auxiliary stage Lamp House Objective 4x, 10x, 20x, 40x Main frame Reflected Fluorescent Mirror Block INVERTED BIOLOGICAL MICROSCOPE 35/54 mm Petri dish holder Main switch Terasaki holder Light intensity control knob BS-7020 Inverted Fluorescence Microscope Fig.2...
  • Page 8: Installation

    BestScope International Limited BS-7020 2. Installation 2-1 Installation Diagram The following figure shows the installation sequence of the components. The number in the figure shows the installation sequence. Before installing, be sure every component is clean, do not score any parts or glass surface.
  • Page 9: Installing Steps

    BestScope International Limited BS-7020 2-2 Installing steps 2-2-1 Installing and replacing the lamp (Fig.4)  Please use the specified halogen Lamp 6V30W. 1. Hold to the bulb ①after you wrap it with gauze or other protection materials, then depress the plugs② into the jack④ on the lamp house, ensure the filament and the bolt③...
  • Page 10: Installing The Lamp House

    BestScope International Limited BS-7020 2-2-2 Installing the lamp house (Fig.5) Keep the BNC connector plugs③ and the lamp house pin② in line, and keep the bolt① and the condenser jack④ in line, too. Then push the lamp house into the illumination unit gently until they are against each other.
  • Page 11: Installing The Stage Lengthen Splint And The Mechanical Ruler

    BestScope International Limited BS-7020 2-2-4 Installing the stage lengthen splint and the mechanical ruler (Fig. 8) ◎ Stage lengthen splint can be installed at either side of the stage to enlarge the work surface. But you can’t install the mechanical ruler together at the same side.
  • Page 12: Installing The Color Filters

    BestScope International Limited BS-7020 Fig.10 2-2-7 Installing the color filters ( Fig.11 ) Let the filters cool down sufficiently before replacing INVERTED BIOLOGICAL MICROSCOPE them. Take out the filter holder① and insert the required filters②. Push the filter down to the bottom as shown in Fig.11 ③...
  • Page 13 BestScope International Limited BS-7020 ④ ② ③ ● Be sure to use a 100W HBO spherical mercury burner. ● Be sure to mount positive pole(the wide head) before the other , or the damage to the burner may occur. ● Never subject the burner to excessive force when mounting the Mercury Burner.
  • Page 14: Connecting The Power Cord

    BestScope International Limited BS-7020 2.2.10 Attaching the protection barrier, stage ② ③ center plate and Fluorescence mirror switching lever (Fig.16) Extend The light shielding plate into the lower part of the platform, then tighten the two clamping screws②. 2. Fit the stage center plate into the opening on the stage.
  • Page 15: Controls

    BestScope International Limited BS-7020 3. Controls Aperture iris diaphragm lever Filter holder Phase contrast slider Light Annulus Centering Bolt Optical path switching knob Fine focusing knob Coarse focusing knob Tension adjustment collar Fig.20...
  • Page 16: Adjustment &Operation

    BestScope International Limited BS-7020 4. Adjustment & Operation 4-1 Transmitted Light Adjustment 4-1-1 Turning on the lamp (Fig.21) Connect the power supply, turn on the main switch①(shown in Fig.21)which on the bottom side of the base to “-”(on). 4-1-2 Adjusting the brightness (Fig.21) Turning the brightness adjustment knob②...
  • Page 17: Using Color Filter

    BestScope International Limited BS-7020 4-1-4 Using color filters (Fig.23) ◎ using appropriate filters according to the purposes allows you to observe and photograph specimens more effectively. Particularly, the use of the LED filter is recommended in observation and photomicrography because it renders more neutral colors.
  • Page 18: Using The Aperyure Diaphragm

    BestScope International Limited BS-7020 Fig.24) 4-1-5 Using the aperture diaphragm ( ◎ the aperture iris diaphragm determines the numerical aperture of the illumination system in bright field observation.Only when the numerical aperture of the objective and the illumination system being matching, you can obtain the higher image resolution and contrast, and the increased depth of focus, too.
  • Page 19: Stage

    BestScope International Limited BS-7020 4-2 Stage 4-2-1 Placing the specimen (Fig.26& Fig.27) Put the specimen in the center of the stage. ★ to obtain the best image effect, please select the containers, such as culture dish and culture bottle, with the bottom thickness of 1.2mm, and the same thickness is also required...
  • Page 20: The Viewing Tube

    BestScope International Limited BS-7020 4-3 The viewing tube 4-3-1 Adjusting the diopter (Fig.28) Look into the right ocular by your right eye, then revolving the coarse focus knob to focus on the specimen. Then use your left eye to look into the left ocular.
  • Page 21: Selecting The Light Path

    BestScope International Limited BS-7020 4-3-3 Selecting the light path ◎Used for digital photography, CCD and 135 camera. ◎ Revolve two knobs in fig.30 respectively to select the light path you need. ◎ when in the binocular observation, turn the knob to the gap position until you heard a “clicked”.
  • Page 22: Adjustment Of Power Supply Unit

    BestScope International Limited BS-7020 4-4 Adjustment of Power Supply Unit (Fig.31) 4-4-1 Power supplier connection Set the main switch of the power supply unit to“—”(ON). The arc will stabilize in 5 to 10 minutes after ignition. ① ② ⚫ Some mercury burners may not ignite the first time the power is turned on due to variance in ③...
  • Page 23: Centering The Mercury Burner

    BestScope International Limited BS-7020 4-4-3 Centering Mercury Burner (Fig.32-33) ◎ before proceeding to center the burner, wait for the arc image to stabilize. to protect against glare during image centering, it should be viewed across the protection barrier. 1. Remove the cap (or objective) from a revolving nosepiece position and engage this position in the light path.
  • Page 24 BestScope International Limited BS-7020 4-4-4 Centering the Field Iris Diaphragm ② (Fig.34-35) 1. Engage the 10X objective in the light path, push in the fluorescent mirror switching lever① to engage the B-excitation mirror into the light path. And set the filter slider the central open position.
  • Page 25: Phase Contrast Observation

    BestScope International Limited BS-7020 5. Phase Contrast Observation 5-1 Name of components 5-1-1 Phase contrast objective (Fig.36) ◎phase contrast objective 10x, 20x, 40x; ◎installation type: refer to 2-2-3: replace the normal objective with phase contrast objective on nosepiece Fig.36 5-1-2 Phase contrast slider (Fig.37) ◎...
  • Page 26: Centering The Light Annulus

    BestScope International Limited BS-7020 Fig.38 (Fig.39-40) 5-2-2 Centering the light annulus Usually you needn’t the operation of centering. If necessary, please accord to the following steps: 1. Place a specimen on the stage and bring it into focus. 2. Replace the eyepiece in the sleeve with the centering telescope.
  • Page 27: Microscope Photography And Video

    BestScope International Limited BS-7020 ★ The centering procedure may have to be repeated in order to get the best possible contrast if a specimen slide or the bottom surface of a culture vessel is not flat. Center the light annulus using objectives in the order of lower to higher magnifications.
  • Page 28: Microscope Photography

    BestScope International Limited BS-7020 6-2 Microscope photography 6-2-1 selecting the light path ★ Just used in the trinocular observation Refer to section 6-1-1 for operation information. More details about light path selection can be obtained in section 4-3-3. 6-2-2 Installing the photography set (Fig.43) 1.
  • Page 29: Digital Photography

    BestScope International Limited BS-7020 6-3 Digital photography 6-3-1 Selecting the light path ★ Just used in the trinocular observation Refer to section 6-1-1 for operation information. More details about light path selection can be obtained in section 4-3-4. Digital Camera Fig.44...
  • Page 30: Specifications

    BestScope International Limited BS-7020 7. Specifications 7-1 Main specifications Optical system Infinite Optical System Seidentopf binocular viewing head Inclined at 45°; Division ratio: 80% of Viewing Tube Binocular Viewing and 20% of Video Viewing & Micrography Eyepiece Wide Field Eyepiece 10X, Linear Field of View : Ф20 mm...
  • Page 31: Objective Specifications

    BestScope International Limited BS-7020 7-2 Objective Specifications numerical working Conjugate Parfocal Coverslip Category aperture distance distance distance magnification thickness (N.A) (mm) (mm) (mm) Infinite Long Working ∞ Distance Plan 0.10 1.2 mm Achromatic Objective ∞ 0.25 1.2mm Infinite Long Working ∞...
  • Page 32: Troubleshooting

    BestScope International Limited BS-7020 8. TROUBLE SHOOTING Under certain conditions, performance of the microscope may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact us for assistance.
  • Page 33 BestScope International Limited light annulus Adjust the light annulus so that condenser does not match the it matches the phase annulus of phase annulus of the objective the objective The light annulus and phase Center them correctly annulus are not centered.
  • Page 34: Product Standard

    BestScope International Limited 5.The main switch can be The mercury burner is not Mount a mercury burner set to on but burner does mounted. not ignite The auto ignition system is Repeated ON-OFF is possible in malfunctioning. this case. Ⅳ. Observation tube 1.

Table of Contents