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Inverted Fluorescent Microscope
BS-7000B
Instruction Manual
This manual is written for Inverted Fluorescence Microscope BS-7000B. For safety and keeping
the best performance, making you familiar with the instrument entirely, it is strongly
recommended that you read this manual carefully before using the microscope.
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Table of Contents
Related Manuals for BestScope BS-7000B
Summary of Contents for BestScope BS-7000B
- Page 1 BS-7000B Instruction Manual This manual is written for Inverted Fluorescence Microscope BS-7000B. For safety and keeping the best performance, making you familiar with the instrument entirely, it is strongly recommended that you read this manual carefully before using the microscope.
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Page 2: Table Of Contents
BestScope International Limited User Notices..........................2 1. Components Name........................ 4 2. Installation..........................5 2.1 Installing diagram......................5 2.2 Installment steps......................6 3. Adjustment and operation....................11 3.1 Lamp adjustment for fluorescence observation............11 3.2 Reflected observing illumination adjustment............17 4. Microscope photography and video.................. 19 4.1 Microscope video...................... -
Page 3: User Notices
BestScope International Limited User Notices Safety Note 1. The epi-fluorescent attachment is a precise instrument. Open the box carefully, and avoid dropping the accessories to ground and causing damage to them. 2. Do keep the instrument out of direct sunlight, high temperature or humidity, dusty and vibrations. - Page 4 BestScope International Limited Maintenance and Storage Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze slightly moistened with a mixture of ether (70%) and alcohol (30%). Since solvents such as ether and alcohol are highly flammable, they must be handled carefully.
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Page 5: Components Name
BestScope International Limited 1. Components Name Trinocular tube head Phase contrast slider Transmitted illumination Eyepiec Illumination bracket Condenser Protection barrier Stage Stage inserted plate Lamp house Nosepiece Objectives 。 4× 。 10× 。 20× 。 40× Body Reflected fluorescence units... -
Page 6: Installation
BestScope International Limited 2. Installation 2.1 Installing diagram The following Figure shows the installation sequence of the components. The number in the Figure shows the installation steps. *Before installing, be sure every components is clean, do not score any parts or glass surface. -
Page 7: Installment Steps
BestScope International Limited 2.2 Installment steps ① 2.2.1 Installing trinocular head(Fig 1) Loosen the setscrew ③ on the microscope body② and insert the trinocular Viewing Head ① into the body correctly, tight the setscrew ③. ③ ② Fig 1 2.2.2 Installing eyepiece(Fig 2) Insert the eyepiece④... - Page 8 BestScope International Limited 2.2.4 Installing lamp house (Fig4, Fig5) Insert the plug A on the lamp house into hole A of power cord, then insert the plug B on the lamp house into the B hole of the condenser till there are against.
- Page 9 BestScope International Limited 2.2.6 Installing the objective (Fig8, Fig9) 1. Turning the coarse focusing knob① like the figure shows till the nosepiece get to its lowest position. ★For ensuring the safety of the instrument during transportation, the nosepiece has been adjusted to the lowest position and the tension adjustment collar②...
- Page 10 BestScope International Limited ● Be sure to use a 100W HBO ultrahigh pressure spherical ② ③ mercury lamp. ● Be sure to mount positive pole(the wide head) before the other , or the damage to the lamp may occur. ● Never subject the lamp to excessive force when mounting the Mercury lamp.
- Page 11 BestScope International Limited 2.2.10 Installing the stage lengthen splint and ① the mechanical ruler (Fig 14, 15) ◎ Stage lengthen splint(auxiliary stage) can be installed in either side of the stage to enlarge the work surface. But you can’t install the mechanical ruler together at same side.
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Page 12: Adjustment And Operation
BestScope International Limited 3. Adjustment and operation 3.1 Lamp adjustment for fluorescence observation 3.1.1 Connecting power Set the main switch of the power supply unit to “I” (ON). It will stabilize in 5 to 10 minutes after ignition. ● Some mercury lamps may not ignite the first time the power is turned on due to variance in production. - Page 13 BestScope International Limited 3.1.4 Adjusting the interpupillar distance (Fig3, When observing with two eyes, hold on the left and right prism holder, turn around the axis, adjusting the interpupillary distance until the left and right fields of view coincide completely.
- Page 14 BestScope International Limited 3.1.7 Centering the mercury lamp (Fig.6-8) ◎Before proceeding to center the mercury lamp, turn on the power supplier, wait for the brightness of the lamp to stabilize. When centering the mercury lamp, to protect users’ eyes, please use the excitation light protective shield (protection barrier).
- Page 15 BestScope International Limited Note: ● When the hour counter indicates “100.0”, set the main switch to “o” (OFF) for safety, wait for more than 10 minutes, then replace the mercury lamp after making sure that the lamp housing has cooled down.
- Page 16 BestScope International Limited Centering the mirror reflected image (Fig.7) ★ The mirror reflected image has been centered before leaving the factory. Please do not adjust the knob⑦ if not necessary. Only when the lamp has been centered precisely, can the knob⑦ be adjusted.
- Page 17 BestScope International Limited 3.1.9 Using color filters (Fig. 11) ◎ Select the appropriate color filters according your need, it became more effective to observe or photography the specimen. Especially, we suggest using the LBD color filter, which can compensate more neutral colors.
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Page 18: Reflected Observing Illumination Adjustment
BestScope International Limited 3.2 Transmitted illumination adjustment 3.2.1 Turn on power, adjusting brightness(Fig 13) Connect the power, turn on the main switch① (shown on the Fig.13) which on the bottom side of the base to ② “ － ”(on). Turning the brightness adjustment knob clockwise②, the voltage raise, and the brightness... - Page 19 BestScope International Limited Using the CT to look the light ring’s image① and the phase contrast ring’s image②, if the light ring’s image is not sharp, please shifting the CT’s ocular until you can see a clear image of the light ring②.
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Page 20: Microscope Photography And Video
BestScope International Limited 4. Microscope photography and video 4.1 Microscope video 4.1.1 Selecting the light path (Fig.1) ★ just used in the trinocular observation 1. Pulling out the light path selector lever, until you heard the “clicked”. ★ In the dark specimen observation, you can make the focus by both eyes at first, then change the light path. -
Page 21: Microscope Photography
BestScope International Limited 4.2 Microscope photography 4.2.1 Selecting the light path ★ just used in the trinocular observation The operation diagram is shown in the details reference is in 4.1.1. 4.2.2 Installing the photography set (Fig.3) Loosen the locking bolt① on the trinocular viewing tube, and take out the dust cap②. -
Page 22: Outfit
BestScope International Limited 5. Outfit 5.1 Specification Optical system Infinite optical system BS-7000B Excitation units Excitation Dichroic mirror Barrier filter ● Blue Excitation BP460～490 DM500 BA520 Reflected light source ● Green Excitation BP510～550 DM570 BA590 ○ BP330～385 Ultraviolet Excitation DM400 BA420 ○... -
Page 23: Objective Specifications
BestScope International Limited 5.2 Objective Specifications NUMERICAL WORKING CONJUGATE FOCUS COVER SLIP Type MAGNIFICATION APERTURE DISTANCE DISTANCE DISTANCE THICKNESS (N.A) (mm) (mm) (mm) Infinite Long Working 25.2 ∞ ─ Distance Plan Achromatic ∞ 1.2mm Objective Infinite Long Working 0.25 ∞... -
Page 24: Troubleshooting
BestScope International Limited 6. Troubleshooting Under certain condition, some no-fault factors will bring a reversible influence to the instrument’s performance. If the problem is happened, please take proper measures according to the follow table. If you can’t solve the trouble by the supplied methods, please contact with the sales department of our company. - Page 25 BestScope International Limited The objective used is not fit to the Please use the compatible objective phase contrast observation When looking at the edge of the Moving the culture dish until you culture dish, the phase contrast ring obtain phase contrast effect. You also...
- Page 26 BestScope International Limited PROBLEM REASON PAGE SOLUTION II. Mechanical Part: 1. The coarse focus The tension adjustment collar is Loose properly knob is hard to run too tight 2. The image can’t stay The tension adjustment collar is on the focal when...
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