Spiral Plate Counting - UVP ColonyDoc-It Instruction Manual

ColonyDoc-It Imaging Station

Spiral Plate Counting

To perform a spiral plate count
click onto the Spiral checkbox
and then select Start Colony
Count.
Next, select from the Automated
Count, Manual Count or User
Defined Template Count.
NOTE: If using the software for
the first time, no templates are
created and the User Defined
Template Count option appears
grey. To create a template, first
proceed through the Manual
Count functions to store settings.
Additional instruction on
performing an Automated Count,
Manual Count, or Template
Count is listed in detail in this
manual.
After performing a count, a
Spiral Plate Analysis window
will appear along with a green
grid over the counted image.
To change the overlay grid size,
move the Overlay Size slider bar
to the left (to decrease the grid
size) or to the right (to increase
the grid size).
To calculate the SPLC/mL, type
in or use the up or down arrows
in the Total volume deposited
box provided. The calculated
amount will appear immediately
below the Calculate SPLC/mL
button.
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First click
onto Spiral
Second click onto
Start Colony Count